Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,272,069	A→G	*419* (TAA→TAG)	hemA →	glutamyl‑tRNA reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,272,069	0	A	G	100.0%	273.0 / NA	83	*419* (TAA→TAG)	hemA	glutamyl‑tRNA reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (34/49);  total (34/49)

AGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGG  >  NZ_CP025268/1271934‑1272106                                                                                                                                         |                                      aGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGt                                   <  2:1391750/140‑1 (MQ=255)     aTTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTAcat                               <  1:3372945/140‑1 (MQ=255)     aTTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTAcat                               >  1:1643254/1‑140 (MQ=255)       tATGCAGGATCTTGCATGGAAACTGACGAACCGCTTGAGCCATGCTCCAAC‑GAAATCACTTCAACAGGCCTCCCGTGACGGGTATAACTAACGCCTGAATATGCTGCGCGACAGCCTCGGTCTGGAGTAGCAGTAcagca                             >  2:2644055/1‑140 (MQ=255)       tATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGCTAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTAcatca                             >  1:3631323/1‑140 (MQ=255)       tATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCCCGGGCTGGAGTAGCAGTAcatca                             >  1:2344133/1‑140 (MQ=255)       tATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCAGAATATTCTGCGCGACAGCCTCGGGCTGGAGCAGCAGTAcatca                             >  2:2589064/1‑140 (MQ=255)          gCAGGATCTGTCATGGGAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAGCGACTTCACCAGGCCGCCCGCGACGGGGCTAACGAACGCCTGAATATCCTGCGCGACAGCCTCGGGCAGGAGTAGCAGTAcatcatgt                          >  2:4276834/1‑138 (MQ=255)          gCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAttt                          <  2:490079/140‑1 (MQ=255)          gCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAttt                          <  1:2239312/140‑1 (MQ=255)          gCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGGACATCAttt                          >  2:4276889/1‑140 (MQ=255)              gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCGAAC‑CAAATCACTGCAACAGGCCGCCCGTGAGGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGCAGCCGTACATCATTTTCtt                      >  2:445021/1‑140 (MQ=255)              gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCGCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGGAGTACATCGTTTGCtt                      >  2:3677437/1‑140 (MQ=255)              gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtt                      <  1:3175927/140‑1 (MQ=255)              gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtt                      >  1:2154163/1‑140 (MQ=255)              gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtt                      >  1:1742094/1‑140 (MQ=255)              gATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAAGCACTTCAACAGACCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtt                      >  2:4791818/1‑140 (MQ=255)                tCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtttt                    >  2:1040625/1‑140 (MQ=255)                tCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtttt                    >  1:2016235/1‑140 (MQ=255)                tCTGGCATGGAAACTGACGAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCtttt                    >  1:2812203/1‑140 (MQ=255)                 cTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttt                   <  2:1822172/140‑1 (MQ=255)                 cTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttt                   <  2:1822622/140‑1 (MQ=255)                 cTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttt                   >  2:2963891/1‑140 (MQ=255)                   ggCCTGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCCCTTCAACAGGCCGCACGTGACGGGGATAACGAACGCCTCAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACACCATCTTCttttttt                 >  2:2790409/1‑140 (MQ=255)                   ggCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttttt                 >  1:3003731/1‑140 (MQ=255)                   ggCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttttt                 >  2:2270177/1‑140 (MQ=255)                   ggCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttttt                 >  1:1838278/1‑140 (MQ=255)                   ggCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttttt                 <  1:242036/140‑1 (MQ=255)                   ggCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttttt                 >  1:234372/1‑140 (MQ=255)                   ggCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttttt                 >  1:4521840/1‑140 (MQ=255)                   ggCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCCTGACGGGGATAACGAATGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCttttttt                 >  1:2380171/1‑140 (MQ=255)                    gCATGGAAACTGACTAACCGCTTGATCCCTGCGCCACC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTa                <  2:2313186/140‑1 (MQ=255)                    gCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTa                <  1:2293831/140‑1 (MQ=255)                    gCATGGAAACCGGACAACCAGCTGACCCATGGGCCCACGGAACTCCCTCCACCAGGCGGCCGGGGCCGGGG‑TTACGCACGGCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTa                <  2:801530/140‑1 (MQ=255)                    gCATAGAAACAGACTTACCGCTTCATCCATGCGCCACC‑GAAACCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTCAACATTCCGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTa                <  2:3408843/140‑1 (MQ=255)                     cATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTAc               >  1:3228686/1‑140 (MQ=255)                     cATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTAc               <  1:4813637/140‑1 (MQ=255)                      ctGGAAACTGACTGACCGCTTGCTCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGCCAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa              <  2:2360885/139‑1 (MQ=255)                      ctGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GCAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa              <  2:2361058/139‑1 (MQ=255)                      atcgAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa              <  2:2375267/137‑1 (MQ=255)                      aTGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa              <  1:4619332/140‑1 (MQ=255)                      aTGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa              <  1:4742034/140‑1 (MQ=255)                      aTGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa              <  1:1048970/140‑1 (MQ=255)                      aTGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa              <  1:4202716/140‑1 (MQ=255)                      aTGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa              <  1:3034707/140‑1 (MQ=255)                      aTGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACa              <  2:3575672/140‑1 (MQ=255)                       tGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAg             <  2:3956375/140‑1 (MQ=255)                        ggAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATACCGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAgg            >  1:3349702/1‑140 (MQ=255)                        ggAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAgg            >  1:3842100/1‑140 (MQ=255)                        ggAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTAAAgg            >  2:781583/1‑140 (MQ=255)                        gaaaaCTAACTAACCGCTTGATCCATGCGCCAAC‑GACATCACTGCAACAGGCAGCCCGTCACGGGGATCACGAACGACTGAATATTCTGCGCGAAAGCCTCGGGCTGGAGTAGAAGTACATCATTTTCTTTTTTTACCgg            <  2:362732/138‑1 (MQ=255)                         gAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGACCGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAggg           <  1:2678525/140‑1 (MQ=255)                         gAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAggg           <  1:3519486/140‑1 (MQ=255)                         gAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAggg           <  1:2678612/140‑1 (MQ=255)                         gAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAggg           <  1:1415412/140‑1 (MQ=255)                         gAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTGTTTACAggg           >  2:3512112/1‑140 (MQ=255)                         gAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTCTTTACAcgg           >  2:3513031/1‑140 (MQ=255)                         gAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGCGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAggg           <  2:3833156/140‑1 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  1:5095017/140‑1 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  2:744824/140‑1 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  2:710340/140‑1 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  2:3862966/140‑1 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  2:2493732/140‑1 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          >  1:1567061/1‑140 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          >  1:1567462/1‑140 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  1:2119690/140‑1 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  1:345805/140‑1 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  1:4186191/140‑1 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  1:751763/140‑1 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  2:2493444/140‑1 (MQ=255)                          aaaCTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  2:2494010/140‑1 (MQ=255)                          aaaCCGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTTAAAAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATAGACTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGt          <  2:1584734/140‑1 (MQ=255)                              tGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTgcat      >  1:1124833/1‑137 (MQ=255)                              tGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACGTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTgcat      >  1:2859476/1‑137 (MQ=255)                               gACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTgcatt     <  1:3675964/140‑5 (MQ=255)                                 cTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTgcattta   <  1:889553/140‑7 (MQ=255)                                 cTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTgcattta   <  1:4997374/140‑7 (MQ=255)                                 cTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTgcattta   <  2:1729118/140‑7 (MQ=255)                                 cTAACCGCGTGTTACATGCGCCAAC‑GAAATCACGTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGGAGCAGTACATCATTTTGTTCTTTCACAGAGTgcgtttg   <  2:2368482/140‑7 (MQ=255)                                  tAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTgcatttac  <  1:3194111/140‑8 (MQ=255)                                  tAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTgcatttac  <  2:573641/140‑8 (MQ=255)                                    aCCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAttt                          >  1:505796/1‑114 (MQ=255)                                    aCCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCAttt                          <  2:505796/114‑1 (MQ=255)                                                                                                                                         |                                      AGCCATTATGCAGGATCTGGCATGGAAACTGACTAACCGCTTGATCCATGCGCCAAC‑GAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAACAGTACATCATTTTCTTTTTTTACAGGGTGGAGGAGG  >  NZ_CP025268/1271934‑1272106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑