BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,001	A→G	noncoding (36/132 nt)	CXP41_RS06835 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,001	0	A	G	99.6%	743.2 / NA	274	noncoding (36/132 nt)	CXP41_RS06835	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (148/125); minor base C (0/1); total (148/126)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 274 total aligned reads displayed.

TGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCG  >  NZ_CP025268/1292866‑1293133

tGCGGCTTCGTATGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                  <  2:1010872/140‑1 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                  <  2:3556515/140‑1 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCCGCACCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                  >  2:1125302/1‑140 (MQ=255)

tGCGGATTAGTTTGAGATTTCCGGTGTTTTTGAAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                  <  2:1453151/140‑1 (MQ=14)

 gCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                                                 <  1:295447/140‑1 (MQ=255)

 gCGGATGCGTTTGAGGATTCGGGGGTTTTTGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                                                 <  2:4417807/140‑1 (MQ=14)

  cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                                >  2:4614662/1‑140 (MQ=255)

  cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                                >  2:2009188/1‑140 (MQ=255)

  cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                                >  2:2117691/1‑140 (MQ=255)

      ttCGTTTGAGAATTCCGGGGCTTTTGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtc                                                                                                                            <  2:2848167/140‑1 (MQ=255)

          tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                                        >  1:1239298/2‑140 (MQ=255)

          tctGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                                        <  2:3439431/138‑1 (MQ=255)

           ttGAGAATTCCGGTGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATc                                                                                                                       <  2:2205035/140‑1 (MQ=255)

               gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                                   <  1:959066/140‑1 (MQ=255)

               gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                                   <  1:2725069/140‑1 (MQ=255)

               gaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                                   <  1:694688/140‑1 (MQ=255)

                    ccGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                              >  2:2359849/1‑140 (MQ=9)

                    ccGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                              <  1:4865400/140‑1 (MQ=37)

                    ccGGGGCCTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCGGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCTGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                              >  2:4044604/1‑140 (MQ=255)

                     cGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                                             <  1:4388563/140‑1 (MQ=37)

                     cGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                                             <  1:2492418/140‑1 (MQ=37)

                        ggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                                        >  1:4337254/1‑126 (MQ=25)

                         gattttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                         <  1:2672508/138‑1 (MQ=18)

                         gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                         <  1:883047/140‑1 (MQ=18)

                         gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                         <  1:1169177/140‑1 (MQ=18)

                           ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                       <  2:3723182/140‑1 (MQ=17)

                           tttaggaaGTGATGGGGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                       <  2:256348/134‑1 (MQ=11)

                             ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                     >  1:4085931/1‑140 (MQ=18)

                             ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                     >  1:3934815/1‑140 (MQ=17)

                                   gTGATGGTGGTGGGGGAAGCACTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                               <  1:412682/140‑1 (MQ=255)

                                     gATGGTGGTGGGGGAAGGATTAGTAAGCTCTACGTGCATCACGCTTGGGGTCGTTGCCTGTGGCAACGCTCTCTCGCTGACGCGCGAGTGGAACCTTAGTCTAAGGTGCTCATCCTTCCCCGCGTGGTCAGAATATTaaa                                                                                             >  2:4424274/1‑140 (MQ=2)

                                               ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGTGGCAACGCTCTCTCGCTGACGCTCGATCCGAACCTTAGTCGAAGGTTCTCATCCTTCCCCGCATGGGCAGAACATTTAATTGCGGATTc                                                                                   >  2:4455392/1‑140 (MQ=11)

                                                    aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                              >  2:762267/1‑140 (MQ=11)

                                                    aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                              >  2:868005/1‑140 (MQ=11)

                                                    aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                              >  1:2413653/1‑140 (MQ=255)

                                                    aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                              >  1:1264710/1‑140 (MQ=11)

                                                    aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGGCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                              >  1:772177/1‑140 (MQ=11)

                                                     ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGGGGATTCGTgggg                                                                             >  1:218465/1‑140 (MQ=11)

                                                     ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTggg                                                                             <  2:1813685/140‑1 (MQ=11)

                                                     ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTggg                                                                             <  1:2359849/140‑1 (MQ=11)

                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                            <  1:1557890/140‑1 (MQ=11)

                                                              aGCGCTGCGCGCTTCGCCCTTCGTGTCGTTACCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                                    >  1:3063377/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCTCGCATGGGCAGAATATTTAATCGCGGATTCGTTGGGAAGTTCAgggg                                                                  >  2:941716/1‑139 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  1:1065279/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  1:1415939/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  1:1125102/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  2:4884370/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  2:2698561/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  2:2331901/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  1:317868/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  1:808496/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  1:3697304/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  1:3356691/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  2:1643194/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                  >  2:1335786/1‑140 (MQ=14)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGCTGGGAAGTGCAgggg                                                                  >  2:3767863/1‑139 (MQ=11)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTACCCGCATGTGCAGAATATTTAATTGCGGATTCGTTGTGTAGTTGAgggg                                                                  >  2:5075719/1‑139 (MQ=11)

                                                                cgcTGCGCGCTTCGCCCTTCGGGCCGTTGCCTGCGGCAACGCTCGCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGGAAGTTCAGGGa                                                                  >  2:4924465/1‑140 (MQ=11)

                                                                 gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTGGAGCCGAAGCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTCGGGAAGTTCAGGGat                                                                 >  2:5089836/1‑139 (MQ=14)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGGAGAAGATTTACTTGCGGATCCGTGGGGAAGTTAAAGGACtttt                                                             >  2:1919431/1‑140 (MQ=14)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTACTAGCGGACTCGATGTGAAGTTCACGGACtttt                                                             >  2:3309418/1‑140 (MQ=11)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                             <  2:4575869/140‑1 (MQ=14)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCACCCTTCCCCGCATGGTGAGAATATTTAATAGCTGATTCGTTGGGAAGTTGAGGGACtttg                                                             >  2:1704144/1‑139 (MQ=14)

                                                                      gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                            >  1:455405/1‑140 (MQ=14)

                                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                          <  1:1483950/140‑1 (MQ=255)

                                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                          <  1:3417341/140‑1 (MQ=14)

                                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                          <  1:2898150/140‑1 (MQ=14)

                                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                                        >  1:480210/1‑140 (MQ=14)

                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGA                                                     <  1:3626119‑M1/140‑1 (MQ=255)

                                                                              cggcgccggTCGTTGCCTGCAGCAACGCTCTCGCGCGCGCGCTCGGGTCTATCTATGGGCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAt                                                    <  2:3211279/133‑1 (MQ=14)

                                                                               ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATG                                                   <  1:4451371‑M1/140‑1 (MQ=255)

                                                                                  tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTG                                                >  1:634162‑M1/1‑140 (MQ=255)

                                                                                   cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGG                                               <  2:2975192‑M1/140‑1 (MQ=255)

                                                                                     ggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTG                                             <  2:3260442‑M1/140‑1 (MQ=255)

                                                                                      gTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGG                                            >  1:1955938‑M1/1‑140 (MQ=255)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGG                                         >  1:269913‑M1/1‑140 (MQ=255)

                                                                                          ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                        <  1:338073‑M1/140‑1 (MQ=255)

                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTggg                                                                             <  1:2137978/102‑1 (MQ=11)

                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                                       >  1:2981925‑M1/1‑140 (MQ=255)

                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                                       <  1:379605‑M1/140‑1 (MQ=255)

                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                                       <  1:1658364‑M1/140‑1 (MQ=255)

                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTggg                                                                             >  2:2138723/1‑102 (MQ=11)

                                                                                             ccgcaggcaacgCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGG                                     >  1:381693‑M1/6‑140 (MQ=255)

                                                                                               tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAT                                   >  1:55724‑M1/1‑140 (MQ=255)

                                                                                               tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAT                                   >  1:1932389‑M1/1‑140 (MQ=255)

                                                                                                gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATT                                  >  2:832176‑M1/1‑140 (MQ=255)

                                                                                                gCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATT                                  >  2:265560‑M1/1‑140 (MQ=255)

                                                                                                  ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcg                                <  1:4834316‑M1/140‑3 (MQ=255)

                                                                                                    cAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaa                              >  1:1219059‑M1/1‑136 (MQ=255)

                                                                                                       cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaacct                           >  2:664430‑M1/1‑133 (MQ=255)

                                                                                                       cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaacct                           >  1:3590379‑M1/1‑133 (MQ=255)

                                                                                                                  tggcgccCGAGTCGAACCTTGGTCGAAGCTACTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGTGGGAAGGATTcgaaccttcgaagtcgat                <  2:1532922‑M1/137‑19 (MQ=255)

                                                                                                                  tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTggg                                                                             <  2:4660528/79‑1 (MQ=255)

                                                                                                                  tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTggg                                                                             >  1:4660528/1‑79 (MQ=255)

                                                                                                                      gCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATTGTGGTTGGGGAAGGATTcgaaccttcgaagtcgatgacg            >  2:601853‑M1/1‑118 (MQ=255)

                                                                                                                      gCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacg            >  2:4283070‑M1/1‑118 (MQ=255)

                                                                                                                        tCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggc          >  1:2831239‑M1/1‑116 (MQ=255)

                                                                                                                         cGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggca         <  1:3137430‑M1/140‑26 (MQ=255)

                                                                                                                              cGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggcagattt    <  1:2643031‑M1/140‑31 (MQ=255)

                                                                                                                                aaCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggcagatttac  <  1:1852385‑M1/140‑33 (MQ=255)

TGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCG  >  NZ_CP025268/1292866‑1293133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`