BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,160	G→A	noncoding (55/132 nt)	CXP41_RS06840 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,160	0	G	A	88.0%	375.2 / 22.3	161	noncoding (55/132 nt)	CXP41_RS06840	RtT sRNA
Reads supporting (aligned to +/- strand): ref base G (8/11); new base A (100/39); total (111/50)
Fisher's exact test for biased strand distribution p-value = 1.57e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01

Only 100 of 161 total aligned reads displayed.

CAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGA  >  NZ_CP025268/1293031‑1293296

cAGAATACTTGATTGGGGATTCGTTTGAGGGTTTAGGGAGTGTTGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAgtc                                                                                                                                <  2:2647890/140‑3 (MQ=11)

                                   gggACTTTTGGAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                             <  2:1069497/140‑1 (MQ=11)

                                        tttagtaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                        <  2:3288111/134‑1 (MQ=11)

                                        tttaggaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                        <  1:4294587/134‑1 (MQ=1)

                                                gTGATGGTGGTGGGGGAAGGATTACTCATCGCTTCGCGCTTCGCCCTGCGGCTCGTTGCCTGCTGCCGCGGCCTCCCGCTGGCGCGCGAGTCGACCCGTGGTTGAAGCTTCTCTTACTTCCCCGCATGGGCAGAATAttt                                                                                >  2:4103767/1‑140 (MQ=2)

                                                                      tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGt                                                          >  1:1237758/1‑140 (MQ=9)

                                                                      tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGt                                                          >  1:950608/1‑140 (MQ=14)

                                                                        cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                        >  2:4482799/1‑140 (MQ=17)

                                                                        cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                        >  1:980375/1‑140 (MQ=16)

                                                                        cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                        >  1:3431307/1‑140 (MQ=12)

                                                                        cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTc                                                        >  2:3108872/1‑140 (MQ=12)

                                                                           aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                                     <  1:1604351/140‑1 (MQ=255)

                                                                             cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCTGATTCGCTTGAGAGTTCAGGGa                                                   >  1:2226658/1‑140 (MQ=255)

                                                                             cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTTTAGAGTTCAGGGa                                                   >  1:1751756/1‑140 (MQ=11)

                                                                             cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                   >  2:3270273/1‑140 (MQ=255)

                                                                             cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                   >  2:3472/1‑140 (MQ=255)

                                                                             cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                   >  1:4735096/1‑140 (MQ=255)

                                                                             cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                   >  1:1665459/1‑140 (MQ=255)

                                                                             cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                   >  1:4953563/1‑140 (MQ=255)

                                                                             cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTTCGGATCCGCTTGACAGTTCggggc                                                   >  2:2122346/1‑139 (MQ=11)

                                                                             cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCGCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGCTTGAGAGTTCAGGGa                                                   >  2:3832312/1‑140 (MQ=255)

                                                                             cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAACTTAGTCGAAGCTTCCCACCCTTCCCCGCATGGGGAGAATATTGAATTGCGGATTCGCCTGAGAGTTCAGGGa                                                   >  2:2623/1‑140 (MQ=11)

                                                                             cgcTGCGCGCTTCGCCCCTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                                   <  2:1972099/140‑1 (MQ=255)

                                                                                  cgcgcTTCGCCCTTCGGGTCGTTGCCTGCTGCCACGCTCTCTCGCTGGCGCCCGCGGCGGACCTTTGTGGAGGCTTCTCATCCTTCCCCGCATGGGTAGAATATTTGATTGCGGATTCGTTTGAGAGTTCAGGGACtttt                                              >  2:2932168/1‑140 (MQ=11)

                                                                                  cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCTCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                              >  2:4664443/1‑140 (MQ=255)

                                                                                  cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGGGAGTTCAGGGGCtttt                                              >  2:1730905/1‑140 (MQ=11)

                                                                                  cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGTACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGACAGAATATTTGATTGCTGATTCGCTTGAGAGTTCAGGGACtttt                                              >  2:3051735/1‑140 (MQ=11)

                                                                                  cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGACCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                              >  1:3447872/1‑140 (MQ=255)

                                                                                  cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGCTAGTTCAGGGACtttt                                              >  2:910403/1‑140 (MQ=11)

                                                                                  cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                              >  1:4168029/1‑140 (MQ=255)

                                                                                  cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                              >  1:2343125/1‑140 (MQ=255)

                                                                                  cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                              >  1:4698234/1‑140 (MQ=255)

                                                                                  cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTTTATTTCGGATTCGTTTGAGAGTTCAGAGACtttt                                              >  2:1377472/1‑140 (MQ=11)

                                                                                  cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTCCTCATCCTTCCCCGCAACGGCAGCATATTTGATTGCGGATTTGCTTGAGGGTTCAGGGACcttt                                              >  2:4759570/1‑140 (MQ=11)

                                                                                  cgcgcTTCGCCCTTCGGGCCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTCGATTGCGGATTCGCTTGGGAGTTCAGGGGCtttt                                              >  2:2330621/1‑140 (MQ=11)

                                                                                   gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTg                                             <  2:3581839/140‑1 (MQ=255)

                                                                                     gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                           <  2:3705406/140‑1 (MQ=255)

                                                                                     gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                           <  1:3015703/140‑1 (MQ=255)

                                                                                     gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                           <  1:870087/140‑1 (MQ=255)

                                                                                     gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                           <  2:3542494/140‑1 (MQ=255)

                                                                                     gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                           <  2:3858229/140‑1 (MQ=255)

                                                                                       ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGCATATTTGATTGCGGATTCGCTTGTGAGTTCAGGGACTTTTGAAAg                                         >  1:1204865/1‑140 (MQ=255)

                                                                                       ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                         >  1:687649/1‑140 (MQ=255)

                                                                                         cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCCAGACGAACCTTAGTCGACGCATCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCGTGTGAGTTCAGGGACTTTTGAAAGTg                                       >  1:3050048‑M2/1‑139 (MQ=255)

                                                                                          ggccgtCGGGTCGTTGCGTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGa                                      <  1:655373/135‑1 (MQ=11)

                                                                                          gagcgtCGGGTCGTTGCCTGCGGGAACGCGATCTCGCTGATGCTCGAGCCGAACCTTAGTCGAAGCTTATAATCATTCCCCGCATGGGCAGAATAGTTGATTGCGGATGCGCTTGAGAGTTCAGGGACTGTTGAAAGTGa                                      <  2:2254732/135‑1 (MQ=11)

                                                                                          gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTTAGAGTTCAGGGACTTTTGAAAGTga                                      >  1:3514964‑M2/1‑138 (MQ=255)

                                                                                          gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                      >  2:4186223‑M2/1‑138 (MQ=255)

                                                                                          gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTga                                      >  1:391193‑M2/1‑138 (MQ=255)

                                                                                           cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgat                                     >  1:3347690‑M2/1‑137 (MQ=255)

                                                                                           cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgat                                     >  1:14498‑M2/1‑137 (MQ=255)

                                                                                            ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGGCTTTTGAAAGTgatg                                    <  1:363459‑M2/140‑5 (MQ=255)

                                                                                            ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                    >  1:2394635‑M2/1‑136 (MQ=255)

                                                                                            ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatg                                    >  1:2614738‑M2/1‑136 (MQ=255)

                                                                                              ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggt                                  <  2:2026488‑M2/140‑7 (MQ=255)

                                                                                               tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtg                                 <  1:3125115‑M2/140‑8 (MQ=255)

                                                                                               tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtg                                 <  1:5050794‑M2/140‑8 (MQ=255)

                                                                                               tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGACTCGCTTGGCAGTTCAGGGACTTTCGAAAGTgatggtg                                 <  2:4427170‑M2/140‑8 (MQ=255)

                                                                                               tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCCATCCTTAGTCGAAGCTTCTCATCCTTACCGGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtg                                 >  2:2136444‑M2/1‑133 (MQ=255)

                                                                                                ggggtCGGTGCCTGCGGCAACGGTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCACGCATGGGCAGAATATTTAATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtgg                                >  2:2825153‑M2/2‑132 (MQ=255)

                                                                                                cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtgg                                >  2:1799412‑M2/1‑132 (MQ=255)

                                                                                                 gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                               >  2:2127265‑M2/1‑131 (MQ=255)

                                                                                                 gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggt                               <  1:2109811‑M2/140‑10 (MQ=255)

                                                                                                   gTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgg                             <  2:2022540‑M2/140‑12 (MQ=255)

                                                                                                    tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCTGAGCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTCGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggg                            >  2:4671642‑M2/1‑128 (MQ=255)

                                                                                                    tCGGTGCGTTCGGCAACGCTCTCTCGCTGACGCGCTGGTCGAAGCTTAGTCGGCGCTTCTCATCCTGCCCCGCGTGGGCAGGGTATTTGATTGGGGATTCGCTTGAGAGTTCGGGGACTTTTGAAAGTGATGGTGGTggg                            <  2:3942607/140‑1 (MQ=2)

                                                                                                      gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                          >  1:1834295‑M2/1‑126 (MQ=255)

                                                                                                      gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                          >  2:2860951‑M2/1‑126 (MQ=255)

                                                                                                      gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAGCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                          >  2:3326382‑M2/1‑126 (MQ=255)

                                                                                                      gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACGTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                          <  1:3576254‑M2/140‑15 (MQ=255)

                                                                                                      gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                          >  1:3695407‑M2/1‑126 (MQ=255)

                                                                                                      gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                          >  1:1155825‑M2/1‑126 (MQ=255)

                                                                                                      gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                          <  2:4332460‑M2/140‑15 (MQ=255)

                                                                                                      gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                          >  1:446430‑M2/1‑126 (MQ=255)

                                                                                                      gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                          >  1:186988‑M2/1‑126 (MQ=255)

                                                                                                      gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAAACTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCTGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtggggg                          >  1:2352969‑M2/1‑126 (MQ=255)

                                                                                                        tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaa                        <  1:2245833‑M2/140‑17 (MQ=255)

                                                                                                        tGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaa                        <  1:3162357‑M2/140‑17 (MQ=255)

                                                                                                         gCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaag                       >  1:2774977‑M2/1‑123 (MQ=255)

                                                                                                           cTGCGGCCACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGAAAGTgatggtggtgggggaagga                     >  2:2564081‑M2/1‑121 (MQ=255)

                                                                                                           cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                     >  1:2519263‑M2/1‑121 (MQ=255)

                                                                                                           cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                     >  1:525039‑M2/1‑121 (MQ=255)

                                                                                                           cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                     >  1:1944585‑M2/1‑121 (MQ=255)

                                                                                                           cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                     >  2:551592‑M2/1‑121 (MQ=255)

                                                                                                           cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCGTGGGGGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                     >  2:3323534‑M2/1‑121 (MQ=255)

                                                                                                           cTGCGGAAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaagga                     >  1:2674590‑M2/1‑121 (MQ=255)

                                                                                                           cTGCGGAAACGATCTATCGCTGCTGATCGAGCCGAAACTTGGGCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATGTTTGATTGCGGATTCGCTTGAGAGTTCAGGCACTTTTGAAAGTgatggtggtgggggaagga                     >  2:1466910‑M2/1‑121 (MQ=255)

                                                                                                            tGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCTGATTCGCCCGAGAGATGAGGCCCATTTACAAGTGAGGGTGGTGGTGGACGGag                    >  2:1574397/1‑139 (MQ=11)

                                                                                                            tGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtgggggggggaggat                    <  2:3086802‑M2/140‑21 (MQ=255)

                                                                                                                gCAGCGCTCTCTCGCTGCCGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTACCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGAAAGTgatggtggtgggggaaggattact                >  2:4242796‑M2/1‑116 (MQ=255)

                                                                                                                gCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGTGTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                >  1:807392‑M2/1‑116 (MQ=255)

                                                                                                                gCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATACTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattact                <  2:1143885‑M2/140‑25 (MQ=255)

                                                                                                                 cAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactc               <  1:3845337‑M2/140‑26 (MQ=255)

                                                                                                                        ctctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctg        >  1:4468030‑M2/1‑108 (MQ=255)

                                                                                                                         tctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgc       >  1:4614662‑M2/1‑107 (MQ=255)

                                                                                                                          ctcGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGTGCCTTTTGAGAGTgatggtggggggagaaggattactcagcgctgcg      >  1:4071300‑M2/1‑106 (MQ=255)

                                                                                                                           tcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCCTACCTTCGACGt     >  2:1254577/1‑140 (MQ=14)

                                                                                                                            cGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcg    >  1:1089451‑M2/1‑104 (MQ=255)

                                                                                                                              cTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTgatggtggtgggggaaggattactcagcgctgcgcgct  <  1:4203014‑M2/140‑39 (MQ=255)

                                                                                                                              cTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAGATTTGAGTGCGGATTCGCGTGAGCGTTCAGGGACTTTTGAAAGTgagggtggtgggggaaggagtacgcagcgctgagcggt  <  2:4993537‑M2/140‑39 (MQ=255)

CAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGA  >  NZ_CP025268/1293031‑1293296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`