BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,394,060	T→C	pseudogene (313/966 nt)	ycjG →	L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,394,060	0	T	C	99.7%	955.7 / NA	286	pseudogene (313/966 nt)	ycjG	L‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (133/151); minor base A (0/1); total (134/152)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 287 total aligned reads displayed.

GCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACTGAAAG  >  NZ_CP025268/1393925‑1394195

gcgcAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATc                                                                                                                                     >  2:5103132/1‑140 (MQ=255)

gcgcAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATc                                                                                                                                     <  2:3123809/140‑1 (MQ=255)

 cgcAAATTATGAGCGTCGTGCCGCAACTAGAGTAAGGGCTGAGACGGGAGGTGTTTCAGTAAATTCTCTCTGGCGGCGCAGCACGTAATGCGGTGGAGTGTGCATTGTGGGATTTGGCCGCGCGAGGATAGCAGGAAtgg                                                                                                                                    >  2:1310522/1‑138 (MQ=255)

 cgcAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCg                                                                                                                                    >  2:2969593/1‑140 (MQ=255)

     aaTTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCgctgg                                                                                                                                <  1:430255/140‑1 (MQ=255)

     aaTTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCgctgg                                                                                                                                <  1:2397681/140‑1 (MQ=255)

     aaTTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCgctgg                                                                                                                                <  2:3356018/140‑1 (MQ=255)

     aaTTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCgctgg                                                                                                                                <  1:2110866/140‑1 (MQ=255)

               gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCACAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  2:1105748/140‑1 (MQ=255)

               gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  2:1648008/140‑1 (MQ=255)

               gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  1:1599620/140‑1 (MQ=255)

               gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  2:3866020/140‑1 (MQ=255)

               gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  1:2446447/140‑1 (MQ=255)

               gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  2:2134631/140‑1 (MQ=255)

               gtcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  2:3717095/140‑1 (MQ=255)

               gtcgtGCCCCAACTGGAGAAAGGGCTTACAAGGGCGAAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGGGCAACGACAGCGGCAATCGCTGGCTGATTTGAt                                                                                                                      <  2:2602812/140‑1 (MQ=255)

               gccgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAACAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGAt                                                                                                                      <  1:4092022/138‑1 (MQ=255)

                tcgtGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATc                                                                                                                     >  1:1940328/1‑140 (MQ=255)

                        cAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAAc                                                                                                             >  2:120571/1‑140 (MQ=255)

                         aaCTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACg                                                                                                            <  2:1253520/140‑1 (MQ=255)

                            aagagAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGAGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCtt                                                                                                         <  1:4496421/139‑1 (MQ=255)

                                  aaGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                                   <  1:982016/140‑1 (MQ=255)

                                  aaGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                                   <  1:3479031/140‑1 (MQ=255)

                                  aaGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGAGTTGATCGGCATAACGCTTCCCgag                                                                                                   <  1:805750/140‑1 (MQ=255)

                                  aaGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACACCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgag                                                                                                   <  2:3774363/140‑1 (MQ=255)

                                   aGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCgaga                                                                                                  >  1:3540384/1‑140 (MQ=255)

                                   aGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGCCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGCCATAACGCTTCCCgcga                                                                                                  >  2:2030824/1‑140 (MQ=255)

                                     ggCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACa                                                                                                <  1:3707128/140‑1 (MQ=255)

                                     ggCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACa                                                                                                >  1:2947163/1‑140 (MQ=255)

                                      gCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAg                                                                                               >  2:2378040/1‑140 (MQ=255)

                                       cTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGt                                                                                              <  2:4688271/140‑1 (MQ=255)

                                       cTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGt                                                                                              <  1:4801631/140‑1 (MQ=255)

                                        tGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCACGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGtt                                                                                             >  1:2834083/1‑140 (MQ=255)

                                          acacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                                           <  1:3805137/140‑1 (MQ=255)

                                          acacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                                           >  1:964915/1‑140 (MQ=255)

                                          acacGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTAt                                                                                           <  2:3495870/140‑1 (MQ=255)

                                                gaggagTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGc                                                                                     >  1:1364898/1‑140 (MQ=255)

                                                 aggagTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGca                                                                                    >  2:1520990/1‑140 (MQ=255)

                                                 aggagTTACAAAAAATGCACCCTGCCGGGCCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGAGTTGATCGGCATAACGCTTCCCGCGACAGTTATCACTGca                                                                                    <  2:4876956/140‑1 (MQ=255)

                                                  ggagTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGcac                                                                                   >  1:445487/1‑140 (MQ=255)

                                                   gagTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGGTTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGcaca                                                                                  <  1:3024402/140‑1 (MQ=255)

                                                    agTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAg                                                                                 >  1:3234676/1‑140 (MQ=255)

                                                    agTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGATGGATTGTGCATTGTGGGATGTTGCCGCGAGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCACGAGACAGTTATCAccccccag                                                                                 >  2:2827594/1‑133 (MQ=255)

                                                     gTTGCAAAAAATTCTCCCTCGCGGCGCAACACGTAATGCGCTGCATTGTGCGGTGTGGTATCTTGTGGAGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGa                                                                                <  2:2539498/140‑1 (MQ=255)

                                                      ttGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGAc                                                                               >  1:418235/1‑140 (MQ=255)

                                                        gCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACgg                                                                             >  2:4243732/1‑140 (MQ=255)

                                                             acaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTc                                                                        <  2:2219703/138‑1 (MQ=255)

                                                             aaaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTc                                                                        <  2:947290/140‑1 (MQ=255)

                                                             aaaTTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTc                                                                        <  1:2167639/140‑1 (MQ=255)

                                                                  ctcCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                                   <  1:733110/140‑1 (MQ=255)

                                                                  ctcCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                                   <  1:5013528/140‑1 (MQ=255)

                                                                  ctcCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                                   <  2:4756323/140‑1 (MQ=255)

                                                                  ctcCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                                   <  1:4362909/140‑1 (MQ=255)

                                                                  ctcCATGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCgg                                                                   <  2:88685/140‑1 (MQ=255)

                                                                    cccTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGAAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTa                                                                 <  1:4633557/140‑1 (MQ=255)

                                                                    cccTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCGGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGGTATCACTGCACAGACGGTTGTCATCGGTa                                                                 <  1:4860830/140‑1 (MQ=255)

                                                                     ccTTCCGGCGCACCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTAc                                                                <  2:1088680/140‑1 (MQ=255)

                                                                       tcccGGCGCACCACGTCATGCGCTGGACTGTGCATTGTGGCATCAGGCCGCGCGAACACAGCAGCAATCGCTGGCTGCTTTGATCGGCATAACGGTTCCCGAGACAGTGATACCTGCACAGACGGTTGTCATCGGTACGc                                                              <  2:1236123/138‑1 (MQ=255)

                                                                       tGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGc                                                              <  2:1587048/140‑1 (MQ=255)

                                                                             cgcAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATc                                                        <  2:3984148/140‑1 (MQ=255)

                                                                               cagctCGGAAGGCGCTGGATTGTGCCTTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGGTTTCATCGGCATAACGCTTCCGGAGACAGTTATCACTGCACAGACGGTTGTCATCGATACGCCTGATCAg                                                      <  2:4567231/140‑1 (MQ=255)

                                                                               cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  1:1532166/140‑1 (MQ=255)

                                                                               cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  2:4918052/140‑1 (MQ=255)

                                                                               cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  1:1076405/140‑1 (MQ=255)

                                                                               cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  1:1167415/140‑1 (MQ=255)

                                                                               cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  2:3095588/140‑1 (MQ=255)

                                                                               cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  2:2458557/140‑1 (MQ=255)

                                                                               cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGATTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  1:3943521/140‑1 (MQ=255)

                                                                               cagcaCGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACACCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAg                                                      <  2:1811746/140‑1 (MQ=255)

                                                                               cagcaCGGCATGCACTGGACTGTGCATTGTGGTCCTTGGCCGCGCGCAGACACCAGCAATCGCTGGCTGATTTGATCGCCATACCGCTTCCCGCGACAGTTATCACTGCCCAGACGGTTGTCATCGGTAACCCTGATcct                                                      <  2:2573184/140‑3 (MQ=255)

                                                                                    cGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGc                                                 <  2:2140828/140‑1 (MQ=255)

                                                                                     gTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATGTGATCGGCATAAGGCTTCCCGAGACAGTTCTCACTGCACAGACGGTTGTCATCGGTACGCCTGAGCAGATGGcc                                                <  2:3348368/140‑1 (MQ=255)

                                                                                       aaTGCGCCGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCCTAACGCTTCCCGGGACAGTTATCACTGCCCCGAGGGTTGTCATCGGTACGCCTGATCAGGTGGCCaa                                              >  2:2811733/1‑140 (MQ=255)

                                                                                          gcgcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAg                                           <  2:1432551/140‑1 (MQ=255)

                                                                                            gcTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTg                                         >  2:4068409/1‑140 (MQ=255)

                                                                                               ggATTGTGCATTGTGGGATCTGGCCGCGCGAGGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGGTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCag                                      >  2:3882933/1‑139 (MQ=255)

                                                                                                gATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATc                                     >  1:1418254/1‑140 (MQ=255)

                                                                                                  ttGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCaa                                   <  2:4351123/140‑1 (MQ=255)

                                                                                                     tgCATTGTGGGCTCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAaaac                                >  2:3553695/1‑140 (MQ=255)

                                                                                                     tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAacac                                >  1:4056259/1‑140 (MQ=255)

                                                                                                     tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAacac                                >  2:4441012/1‑140 (MQ=255)

                                                                                                     tgCATTGTGGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATACTGCATCAacac                                >  2:5068958/1‑140 (MQ=255)

                                                                                                          tgtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCggg                           >  2:649178/1‑140 (MQ=255)

                                                                                                          tgtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTgg                           >  1:4771199/1‑140 (MQ=255)

                                                                                                          tgtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTgg                           >  1:1818016/1‑140 (MQ=255)

                                                                                                          tgtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTgg                           >  2:2087710/1‑140 (MQ=255)

                                                                                                          tgtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTgg                           >  1:2321185/1‑140 (MQ=255)

                                                                                                          tgtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTgg                           >  1:3469169/1‑140 (MQ=255)

                                                                                                           gtgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAAGACTCTggc                          >  2:709543/1‑140 (MQ=255)

                                                                                                            tgGGATCTGGCCGCGCGAAGACAGCAGCAATTGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCAGCGGTATGTCTGATTCGATGGCCGATAGTGCATCCACACTCTggca                         >  2:2084610/1‑140 (MQ=255)

                                                                                                            tgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggca                         >  2:195176/1‑140 (MQ=255)

                                                                                                            tgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggca                         >  1:1664218/1‑140 (MQ=255)

                                                                                                            tgGGATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggca                         >  1:3289439/1‑140 (MQ=255)

                                                                                                               gATCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTggcaggc                      >  2:321734/1‑140 (MQ=255)

                                                                                                                 tCTGGCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGgcaggcag                    >  2:3291278/1‑140 (MQ=255)

                                                                                                                    ggCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCTGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGGGCATCAACACTCTGGCAGGCAGgcg                 >  1:1509195/1‑140 (MQ=255)

                                                                                                                    ggCCGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGgcg                 >  1:1044215/1‑140 (MQ=255)

                                                                                                                      ccGCGCGAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCACCACTCTGGCAGGCAGGcgcg               >  1:3090549/1‑140 (MQ=255)

                                                                                                                        gcgcgAAGACAGCAGCAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGcgcgca             >  1:2475275/1‑138 (MQ=255)

                                                                                                                                   gcagcaATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACTGAAAg  >  1:4575583/1‑140 (MQ=255)

GCGCAAATTATGAGCGTCGTGCCGCAACTAGAGAAAGGGCTGACACGGGAGGAGTTGCAAAAAATTCTCCCTGCCGGCGCAGCACGTAATGCGCTGGATTGTGCATTGTGGGATCTGGCCGCGCGAAGACAGCAGTAATCGCTGGCTGATTTGATCGGCATAACGCTTCCCGAGACAGTTATCACTGCACAGACGGTTGTCATCGGTACGCCTGATCAGATGGCCAATAGTGCATCAACACTCTGGCAGGCAGGCGCGAAATTACTGAAAG  >  NZ_CP025268/1393925‑1394195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`