BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,394,317	T→C	pseudogene (570/966 nt)	ycjG →	L‑Ala‑D/L‑Glu epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,394,317	0	T	C	99.3%	959.0 / NA	290	pseudogene (570/966 nt)	ycjG	L‑Ala‑D/L‑Glu epimerase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (167/119); total (169/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

AATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGAA  >  NZ_CP025268/1394184‑1394451

    aCTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTgg                                                                                                                              <  2:474831/140‑1 (MQ=255)

    aCTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTgg                                                                                                                              <  1:692468/140‑1 (MQ=255)

    aCTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTgg                                                                                                                              <  1:963739/140‑1 (MQ=255)

    aCTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTgg                                                                                                                              <  1:995586/140‑1 (MQ=255)

      tGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCg                                                                                                                            <  1:2456484/140‑1 (MQ=255)

      tGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCg                                                                                                                            <  1:4543242/140‑1 (MQ=255)

                    gATAACCATCTTATCAGTGAGCGGATGGTGGAAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGc                                                                                                              <  1:4264918/140‑1 (MQ=255)

                     aTAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCg                                                                                                             <  2:536662/140‑1 (MQ=255)

                     aTAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCg                                                                                                             <  2:2695158/140‑1 (MQ=255)

                     aTAACCATCTTATCAGGGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCg                                                                                                             <  2:2645967/140‑1 (MQ=255)

                       aaCCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGAt                                                                                                           <  1:1771761/140‑1 (MQ=255)

                         caaTCTTATCCATCAGCGGTTGGAGCCAATACACACAGCTGTAACAGATGCGACGCTGATCGTTAATACAAATGAATCCTGGCGTGCAGAAGGATTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGAGATGc                                                                                                         <  2:2531134/138‑1 (MQ=255)

                          cATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCt                                                                                                        <  1:2900750/140‑1 (MQ=255)

                              ttATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGaa                                                                                                    >  1:2032846/1‑140 (MQ=255)

                              ttATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGaa                                                                                                    >  1:4457438/1‑140 (MQ=255)

                              ttATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGaa                                                                                                    >  1:1532331/1‑140 (MQ=255)

                                aTCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGaaca                                                                                                  >  2:1532202/1‑140 (MQ=255)

                                   aGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAAcc                                                                                               <  1:2356705/140‑1 (MQ=255)

                                   aGTGAGCGGATGGTGGCAATTCGCACAGCTCTGCCCGATGCGAGGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAAcc                                                                                               >  2:4663930/1‑140 (MQ=255)

                                    gTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCg                                                                                              >  1:2510468/1‑140 (MQ=255)

                                      gAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCt                                                                                            >  2:2125970/1‑140 (MQ=255)

                                      gAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCt                                                                                            >  2:4120871/1‑140 (MQ=255)

                                      gAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCt                                                                                            <  1:2641862/140‑1 (MQ=255)

                                       aGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCtt                                                                                           >  2:1295681/1‑140 (MQ=255)

                                       aGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCtt                                                                                           >  1:1064884/1‑140 (MQ=255)

                                       aGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCtt                                                                                           >  2:352171/1‑140 (MQ=255)

                                       aGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCtt                                                                                           >  1:3902983/1‑140 (MQ=255)

                                       aGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCtt                                                                                           >  2:716132/1‑140 (MQ=255)

                                       aGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCtt                                                                                           >  1:2180117/1‑140 (MQ=255)

                                       aGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCtt                                                                                           >  1:1932004/1‑140 (MQ=255)

                                       aGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCtt                                                                                           >  1:1645110/1‑140 (MQ=255)

                                        gCGGATTGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          <  2:2272827/140‑1 (MQ=255)

                                        gCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          <  1:5038470/140‑1 (MQ=255)

                                        gCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          <  1:2087699/140‑1 (MQ=255)

                                        gCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          <  2:1933362/140‑1 (MQ=255)

                                        gCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          <  2:2944072/140‑1 (MQ=255)

                                        gCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTc                                                                                          <  2:3889707/140‑1 (MQ=255)

                                        gCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCATTGCGATGCTTGAACAACCGCTTc                                                                                          <  2:35909/140‑1 (MQ=255)

                                         cGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGATGGCGGCGCGTTGCCAGCTATTGGCGGAGTTAGGCGTTGCGATGCTTGAACAACCGCTTcc                                                                                         >  1:3548400/1‑140 (MQ=255)

                                           gATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTg                                                                                       >  2:3259938/1‑140 (MQ=255)

                                             tggtggCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTgcg                                                                                     <  2:2916649/140‑1 (MQ=255)

                                             tggtggCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTgcg                                                                                     <  1:3096034/140‑1 (MQ=255)

                                              ggtggCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTgcgc                                                                                    >  1:2065648/1‑140 (MQ=255)

                                              ggtggCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTgcgc                                                                                    >  1:2767421/1‑140 (MQ=255)

                                               gtggCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCa                                                                                   >  1:922676/1‑140 (MQ=255)

                                                 ggCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAgg                                                                                 >  2:276913/1‑140 (MQ=255)

                                                  gCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGa                                                                                <  2:2321185/140‑1 (MQ=255)

                                                   cAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGAc                                                                               >  1:2995280/1‑140 (MQ=255)

                                                      ttCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGAGGCTTGAACAACCGCTTCCTGCGCAGGACGAt                                                                            >  1:1728257/1‑140 (MQ=255)

                                                       tCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATg                                                                           <  1:4409675/140‑1 (MQ=255)

                                                       tCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATg                                                                           <  1:2590106/140‑1 (MQ=255)

                                                          cacaGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATgcgg                                                                        >  2:3345289/1‑140 (MQ=255)

                                                          cacaGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATgcgg                                                                        >  1:2395670/1‑140 (MQ=255)

                                                          cacaGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATgcgg                                                                        >  1:4024016/1‑140 (MQ=255)

                                                            caGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATgcggcg                                                                      <  1:1017642/140‑1 (MQ=255)

                                                            caGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATgcggcg                                                                      <  1:2691412/140‑1 (MQ=255)

                                                              gCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCAATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTGCCTGCGCAGGACGATGCGGCGCt                                                                    >  2:3024090/1‑140 (MQ=255)

                                                               cTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTg                                                                   >  1:1863097/1‑140 (MQ=255)

                                                                  tgCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGgag                                                                >  1:363377/1‑140 (MQ=255)

                                                                    cccGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCTGAAGGGTGGGGGGCGCGTTGCCAGCGATTGGCGGAGTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGAGGATGCGGCGCTGGAGaa                                                              >  2:1060838/1‑140 (MQ=255)

                                                                       gATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAAttt                                                           >  1:3274971/1‑140 (MQ=255)

                                                                         tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTa                                                         >  1:4929420/1‑140 (MQ=255)

                                                                         tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTa                                                         >  2:3090572/1‑140 (MQ=255)

                                                                         tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTa                                                         >  2:3276843/1‑140 (MQ=255)

                                                                         tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTa                                                         >  1:4835638/1‑140 (MQ=255)

                                                                         tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTa                                                         >  2:4328949/1‑140 (MQ=255)

                                                                         tGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTa                                                         >  1:1243594/1‑140 (MQ=255)

                                                                         tGCGACGCTGATAGTTGATGCAAATGAATCCTGGCGTCCAGAAGGGTTGGCGGCGCGGTGCCACCGAGTGGCGGATTGAGTCGTTGTTATGCATGACCATCCGCTTCCTGCGCAGGACGACGCGGCTCTGGAGAGTTTTa                                                         >  2:4177151/1‑140 (MQ=255)

                                                                              cGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCAt                                                    >  1:3004204/1‑140 (MQ=255)

                                                                              cGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCAt                                                    >  1:496709/1‑140 (MQ=255)

                                                                              cGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCAt                                                    >  1:1391797/1‑140 (MQ=255)

                                                                                 tGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCg                                                 <  1:1242879/140‑1 (MQ=255)

                                                                                        tGATGCTAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGa                                          >  2:4523835/1‑140 (MQ=255)

                                                                                             cAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTtgt                                     >  2:492250/1‑140 (MQ=255)

                                                                                              aaaTGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTtgtg                                    <  2:2180117/140‑1 (MQ=255)

                                                                                               aaTGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGc                                   >  1:3291693/1‑140 (MQ=255)

                                                                                                aTGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCt                                  >  1:5528/1‑140 (MQ=255)

                                                                                                   aaTCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCtgat                               <  2:1645110/140‑1 (MQ=255)

                                                                                                      ccTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGaa                            <  2:4203883/140‑1 (MQ=255)

                                                                                                      ccTGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGaa                            <  1:4294255/140‑1 (MQ=255)

                                                                                                        tGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAg                          <  2:1243439/140‑1 (MQ=255)

                                                                                                        tGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAg                          <  2:1243594/140‑1 (MQ=255)

                                                                                                        tGGCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAg                          <  1:3492693/140‑1 (MQ=255)

                                                                                                          gCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGtt                        >  1:3542218/1‑140 (MQ=255)

                                                                                                          gCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGtt                        >  1:1545006/1‑140 (MQ=255)

                                                                                                          gCGTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGtt                        >  1:223778/1‑140 (MQ=255)

                                                                                                            gTGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGt                      >  1:467232/1‑140 (MQ=255)

                                                                                                             tGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTc                     <  1:321734/140‑1 (MQ=255)

                                                                                                             tGCAGAAGGGTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTc                     >  2:132590/1‑140 (MQ=255)

                                                                                                             tGCAGAAGGGTTGGCGGCGCGTTGACAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTc                     <  1:2137965/140‑1 (MQ=255)

                                                                                                                     ggTTGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGt             <  2:2029594/140‑1 (MQ=255)

                                                                                                                       ttGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAg           <  1:4731616/140‑1 (MQ=255)

                                                                                                                       ttGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAg           >  2:3716762/1‑140 (MQ=255)

                                                                                                                       ttGGCGGCGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAg           >  1:3787362/1‑140 (MQ=255)

                                                                                                                         ggcggcGCGTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGAGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCa         >  2:1784258/1‑140 (MQ=255)

                                                                                                                               gcgTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGa   <  1:1287320/140‑1 (MQ=255)

                                                                                                                               gcgTTGCCAGCTATTGGCGGATTTAGGCGCTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGCTGAAAGTTGTCATACTCGTAGCAATTTGa   >  2:4087813/1‑140 (MQ=255)

                                                                                                                                ctttGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACCACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGaa  <  2:2875301/138‑1 (MQ=255)

                                                                                                                                cgTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGaa  <  1:4190787/140‑1 (MQ=255)

                                                                                                                                cgTTGCCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGaa  <  1:1147635/140‑1 (MQ=255)

AATTACTGAAAGTGAAGCTGGATAACCATCTTATCAGTGAGCGGATGGTGGCAATTCGCACAGCTGTGCCCGATGCGACGCTGATCGTTGATGCAAATGAATCCTGGCGTGCAGAAGGGTTGGCGGCGCGTTGTCAGCTATTGGCGGATTTAGGCGTTGCGATGCTTGAACAACCGCTTCCTGCGCAGGACGATGCGGCGCTGGAGAATTTTATTCATCCGTTGCCGATTTGTGCTGATGAAAGTTGTCATACTCGTAGCAATTTGAA  >  NZ_CP025268/1394184‑1394451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`