BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,469,308	A→G	317 (TAA→TAG) N7D (AAC→GAC)	paaX → paaY →	phenylacetic acid degradation operon negative regulatory protein PaaX phenylacetic acid degradation protein PaaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,469,308	0	A	G	99.5%	703.5 / NA	215	317 (TAA→TAG) N7D (AAC→GAC)	paaX paaY	phenylacetic acid degradation operon negative regulatory protein PaaX phenylacetic acid degradation protein PaaY
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (73/141); minor base T (1/0); total (74/141)
Fisher's exact test for biased strand distribution p-value = 3.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 215 total aligned reads displayed.

CCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGT  >  NZ_CP025268/1469174‑1469444

ccTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGt                                                                                                                                     <  1:2149916/140‑1 (MQ=255)

ccTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGt                                                                                                                                     <  2:2815228/140‑1 (MQ=255)

ccTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGt                                                                                                                                     <  2:3412807/140‑1 (MQ=255)

ccTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGt                                                                                                                                     <  2:2857754/140‑1 (MQ=255)

ccTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATACTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGt                                                                                                                                     <  1:4392556/140‑1 (MQ=255)

ccTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCCGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGTCTTGAATATTGAACAGGAGGAGTTATGCCAATTTATCAGATAGACGGt                                                                                                                                     <  2:3747329/140‑1 (MQ=255)

ccTGCTGCTTTAGCGTACGTTAGTGAAAGAGGCGAAACCTCGGTCGGTGAACTGCCTGCGCCGCGAAGCCTGTATTTTCGACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACggc                                                                                                                                     <  2:4220682/140‑2 (MQ=255)

ccTGCGGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAATCTCGGTCGGGGAACGGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGTCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGt                                                                                                                                     <  2:3535732/140‑1 (MQ=255)

      gctTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACt                                                                                                                               <  1:1077221/140‑1 (MQ=255)

         ttAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCg                                                                                                                            >  2:1502368/1‑140 (MQ=255)

           aacgtgcgttACGGAAATAGGTGTAACCTCGGGCGGTGAACTGCCTGCGCCGTGAAGCCTGTATTTTCTACGTTTTAGCGGCTTGAATATTGAACGGGAGGCGTTATGCCAATTTATCAGATAGACTGTCTGACTCCGGt                                                                                                                          <  2:2238378/138‑1 (MQ=255)

           aGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGt                                                                                                                          <  1:4674423/140‑1 (MQ=255)

           aGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGt                                                                                                                          <  1:1195043/140‑1 (MQ=255)

           aGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGt                                                                                                                          <  1:2455964/140‑1 (MQ=255)

           aGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGCGAAGCCTGTAGTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGt                                                                                                                          <  1:4753210/140‑1 (MQ=255)

               ttcgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTtgtg                                                                                                                      >  2:459702/1‑140 (MQ=255)

               ttcgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTtgtg                                                                                                                      >  2:857477/1‑140 (MQ=255)

                tcgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGc                                                                                                                     <  2:4394410/140‑1 (MQ=255)

                tcgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGc                                                                                                                     <  1:3402990/140‑1 (MQ=255)

                 cgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGcc                                                                                                                    >  1:1935104/1‑140 (MQ=255)

                 cgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGcc                                                                                                                    >  1:1607329/1‑140 (MQ=255)

                 cgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGcc                                                                                                                    >  2:283603/1‑140 (MQ=255)

                 cgttAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGcc                                                                                                                    >  1:1709305/1‑140 (MQ=255)

                 cgtgAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGGCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAAGATATGAGATAGACGGTCTGACTCCGGTTGTGcc                                                                                                                    >  2:4238507/1‑140 (MQ=255)

                      gTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCagaag                                                                                                               <  2:4293378/140‑1 (MQ=255)

                       tGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCagaaga                                                                                                              >  2:124027/1‑140 (MQ=255)

                         aaaaaGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAagaga                                                                                                            >  1:428165/1‑140 (MQ=255)

                                tGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTAAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGt                                                                                                     <  2:2665669/140‑1 (MQ=255)

                                 gtaaCCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATAGTGACCAGGAGGCGTTATGCCAATTTATCAGATAGCCGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTc                                                                                                    <  2:2113130/138‑1 (MQ=255)

                                 gAACCCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGGTATGCCAATTTATCAGATAGGCGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTc                                                                                                    <  2:3353689/140‑1 (MQ=255)

                                 gAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTc                                                                                                    <  2:2850722/140‑1 (MQ=255)

                                   aaCCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCa                                                                                                  >  2:5085108/1‑140 (MQ=255)

                                        cggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGa                                                                                             <  1:4080973/140‑1 (MQ=255)

                                         ggtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGAc                                                                                            >  1:3827220/1‑140 (MQ=255)

                                          gtcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACa                                                                                           <  2:233926/140‑1 (MQ=255)

                                           tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                          <  1:3940525/140‑1 (MQ=255)

                                           tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                          <  1:3510472/140‑1 (MQ=255)

                                           tcggtGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAg                                                                                          <  2:402301/140‑1 (MQ=255)

                                           tcggtGAACTGCCTGCGCCGGGAACCCTGTATTTTCGACGTTTTGTCGGCTTGAATAATGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGCCTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCAGACAg                                                                                          <  2:1825722/140‑1 (MQ=255)

                                                     gcctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGAt                                                                                >  2:1845967/1‑140 (MQ=255)

                                                     gcctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGAt                                                                                >  1:1063727/1‑140 (MQ=255)

                                                     gcctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGAt                                                                                >  1:3011653/1‑140 (MQ=255)

                                                     gcctgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCAGGATGTGCCAGAAGAGAGGTTTGTCCATCCGACAGCGGTATTGAt                                                                                >  2:479750/1‑140 (MQ=255)

                                                        tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGCGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             <  2:1171623/140‑1 (MQ=255)

                                                        tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             >  1:4538594/1‑140 (MQ=255)

                                                        tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             <  2:1308811/140‑1 (MQ=255)

                                                        tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             <  1:4557995/140‑1 (MQ=255)

                                                        tgcGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCgg                                                                             <  2:3123495/140‑1 (MQ=255)

                                                         gcgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGc                                                                            >  2:4340916/1‑140 (MQ=255)

                                                          cgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCg                                                                           <  1:661727/140‑1 (MQ=255)

                                                          cgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCg                                                                           <  2:2184083/140‑1 (MQ=255)

                                                          cgcCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCg                                                                           <  1:1858982/140‑1 (MQ=255)

                                                             cGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATg                                                                        <  1:1299319/140‑1 (MQ=255)

                                                                gAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTa                                                                     >  1:3906710/1‑140 (MQ=255)

                                                                gAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTa                                                                     >  2:5024086/1‑140 (MQ=255)

                                                                  aGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTAtt                                                                   <  2:3675302/140‑1 (MQ=255)

                                                                    ccTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATtct                                                                 <  2:27697/140‑1 (MQ=255)

                                                                         aTTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCa                                                            <  1:5054943/140‑1 (MQ=255)

                                                                          ttttCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCACTTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCaa                                                           <  1:3187858/140‑1 (MQ=255)

                                                                           tctctaCGTTTTGGCGGCTTGAATATTGTGCAGGAGGGGGTATGCCCATTTATCAGATGGCCGGTCTGGCTTCGGTGGAGCCAGAAGGGTGTTGTGTCCAGCCGACAGCGGTATCGACCGGCCATGTTATTTTCGGCAAg                                                          <  2:4059066/135‑1 (MQ=255)

                                                                            ttCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAgg                                                         <  2:191991/140‑1 (MQ=255)

                                                                            ttCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAgg                                                         <  1:1568276/140‑1 (MQ=255)

                                                                             tCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAggg                                                        >  1:766702/1‑140 (MQ=255)

                                                                               aaCGTTTTGGCGGCTTGACTATTGAACAGGAGGCGTTATGCGAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCg                                                      >  1:531336/1‑140 (MQ=255)

                                                                                aCGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGt                                                     <  1:4134555/140‑1 (MQ=255)

                                                                                  gTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGttt                                                   >  2:1374056/1‑140 (MQ=255)

                                                                                    tttGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTGTTGATCGGCGATGTTATTCTCTGCAAGGGCGTTTAc                                                 >  1:1449585/1‑140 (MQ=255)

                                                                                     ttGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACg                                                <  1:1267570/140‑1 (MQ=255)

                                                                                       ggcggcTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGtt                                              <  2:1228658/140‑1 (MQ=255)

                                                                                       ggcggcTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGAGCGGCGATGTTATTCTCGGCAAGGGCGTTTACGtt                                              <  2:2351247/140‑1 (MQ=255)

                                                                                        gcggcTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTg                                             <  1:2336911/140‑1 (MQ=255)

                                                                                          ggcTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTggg                                           <  2:1020819/140‑1 (MQ=255)

                                                                                            cTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGcc                                         <  2:2878808/140‑1 (MQ=255)

                                                                                                 atatTGAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATg                                    <  1:2489317/140‑1 (MQ=255)

                                                                                                      gAACAGGAGGCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGc                               >  1:2576795/1‑140 (MQ=255)

                                                                                                            gaggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGt                         <  2:1234512/140‑1 (MQ=255)

                                                                                                            gaggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGt                         >  1:55533/1‑140 (MQ=255)

                                                                                                            gaggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGt                         >  1:2020051/1‑140 (MQ=255)

                                                                                                            gaggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGt                         >  2:1994333/1‑140 (MQ=255)

                                                                                                              ggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAATAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTCTTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTgg                       >  1:3043772/1‑140 (MQ=255)

                                                                                                              ggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTgg                       >  2:647053/1‑140 (MQ=255)

                                                                                                              ggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTgg                       <  2:832661/140‑1 (MQ=255)

                                                                                                              ggCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTgg                       >  1:1668512/1‑140 (MQ=255)

                                                                                                               gCGTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTggg                      >  1:4497265/1‑139 (MQ=255)

                                                                                                                 gTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCGAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGa                    >  2:4668776/1‑140 (MQ=255)

                                                                                                                 gTTATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGa                    >  2:2601369/1‑140 (MQ=255)

                                                                                                                  ttctGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCACGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGAt                   <  2:4427969/137‑1 (MQ=255)

                                                                                                                   tATGCCAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGAtt                  <  1:3683087/140‑1 (MQ=255)

                                                                                                                       ccAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTgg              <  2:3490737/140‑1 (MQ=255)

                                                                                                                       ccAATTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTgg              <  1:961116/140‑1 (MQ=255)

                                                                                                                          aTTTATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCg           >  1:2733235/1‑140 (MQ=255)

                                                                                                                           tttATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTAGTCTCGGCAAGGGCGTGGACGTTGGGCCAAATGCCAGCCTGCGTTGCGATTTTGTTCgg          >  1:3142832/1‑139 (MQ=255)

                                                                                                                             tATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTAt        <  2:3470188/140‑1 (MQ=255)

                                                                                                                             tATCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTAt        <  1:4284150/140‑1 (MQ=255)

                                                                                                                              aTCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATc       <  1:3338438/140‑1 (MQ=255)

                                                                                                                               tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGCGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGCTGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg      <  2:4440446/140‑1 (MQ=255)

                                                                                                                               tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg      <  2:1063727/140‑1 (MQ=255)

                                                                                                                               tCAGATAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg      <  1:1395589/140‑1 (MQ=255)

                                                                                                                               tCAGATAGACGGCCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCg      <  1:1723444/140‑1 (MQ=255)

                                                                                                                                   aTAGACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCgtggt  >  1:598991/1‑140 (MQ=255)

CCTGCTGCTTTAGCGTTCGTTAGTGAAAAAGGTGAAACCTCGGTCGGTGAACTGCCTGCGCCGGGAAGCCTGTATTTTCAACGTTTTGGCGGCTTGAATATTGAACAGGAGGCGTTATGCCAATTTATCAGATAAACGGTCTGACTCCGGTTGTGCCAGAAGAGAGTTTTGTCCATCCGACAGCGGTATTGATCGGCGATGTTATTCTCGGCAAGGGCGTTTACGTTGGGCCAAATGCCAGCCTGCGTGGCGATTTTGGTCGTATCGTGGT  >  NZ_CP025268/1469174‑1469444

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`