Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,576,151	T→G	I238I (ATA→ATC)	gadB ←	glutamate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,576,151	0	T	G	98.7%	168.9 / NA	78	I238I (ATA→ATC)	gadB	glutamate decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base G (40/37);  minor base C (0/1);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

GCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAATCA  >  NZ_CP025268/1576072‑1576284                                                                                |                                                                                                                                      gCAGGCTGGAGTCCCAGACGATCTCCGGGGCGACGATCGTCGCCAGCAAGCCCCCGCTGGCAGCGTCGATGTGCATGTCCGTGTCCATGCCGGTATCGGCCTAGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAAc                                                                           <  2:1672807/140‑1 (MQ=11)               cAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAgt                                                             <  2:1731108/140‑1 (MQ=17)               cAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCCGTTGTGGGAACTCATAGTTACCAgt                                                             <  2:4566210/140‑1 (MQ=12)                aGACGATATCCGGGGCGACCAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAgtg                                                            <  1:3749230/140‑1 (MQ=18)                     atatCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGt                                                       <  2:1271343/140‑1 (MQ=18)                      tatCCGGGGCGACGAACGGTTCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGAGGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTc                                                      >  1:1874015/1‑140 (MQ=12)                      tatCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTc                                                      >  1:123125/1‑140 (MQ=18)                      tatCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTc                                                      >  1:1594280/1‑140 (MQ=18)                      tatCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGGAGGTc                                                      >  1:1594165/1‑140 (MQ=12)                      tatCCGGGGCGACGAACGGGGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTGGATACCGGTATCGGCCTGGAATGTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTc                                                      >  2:4258125/1‑140 (MQ=255)                       atCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCGGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCa                                                     >  1:891745/1‑140 (MQ=12)                            gggCGACGAACGGTGCCGGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCg                                                <  2:2422519/140‑1 (MQ=17)                            gggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTGACGCCg                                                >  2:3533890/1‑140 (MQ=12)                            gggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCg                                                <  1:2742257/140‑1 (MQ=18)                            gggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCg                                                <  2:3318508/140‑1 (MQ=18)                            gggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCg                                                <  2:4519220/140‑1 (MQ=18)                            gggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCg                                                <  2:3706537/140‑1 (MQ=18)                             ggCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGa                                               >  2:2189315/1‑140 (MQ=18)                              gCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTCTCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGaa                                              >  2:3827154/1‑140 (MQ=12)                                gacgaACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAg                                            >  1:270584/1‑140 (MQ=18)                                gacgaACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAg                                            >  2:2197218/1‑140 (MQ=18)                                 acgaACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGt                                           <  1:3399256/140‑1 (MQ=17)                                    aaCGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCgg                                        <  2:2503364/140‑1 (MQ=18)                                    aaCGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCgg                                        <  1:2524319/140‑1 (MQ=18)                                     aCGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGc                                       <  2:1089818/140‑1 (MQ=18)                                       ggTGCCAGGAAGCCAGCGCTGGCAGCGTCGATGGGCGTGTCGATGTCGATACCGGTATCGGGCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTGATAGTTACCAGTGTAGGTTACGCCGAAAGTCggtgg                                     >  2:4249951/1‑137 (MQ=11)                                           ccAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACg                                 >  1:3593893/1‑140 (MQ=18)                                               gAAGCCAACGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGa                             <  2:4237160/140‑1 (MQ=18)                                                aaGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGAt                            <  2:185078/140‑1 (MQ=17)                                                 aGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATATATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATg                           >  2:3061279/1‑140 (MQ=16)                                                   ccACCGCTGGCAGGGGCGATGTGAATGTCGATGTAGATACCGGTCGCGGCCAGGAATTTATCAAGCGCATCGTGCAGGGGTTGTGGGAACTCTTCGTTACCAGTGTAGGTCACGCCGAACGTCGGCACAACGcaacgggg                         >  2:2427591/1‑133 (MQ=255)                                                   ccACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGTTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                         >  2:1027778/1‑140 (MQ=12)                                                   ccACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                         >  2:2025509/1‑140 (MQ=17)                                                   ccACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                         >  1:4443302/1‑140 (MQ=18)                                                   ccACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                         >  2:3204689/1‑140 (MQ=18)                                                   ccACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                         >  1:786095/1‑140 (MQ=18)                                                   ccACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                         >  1:1680586/1‑140 (MQ=18)                                                   ccACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                         >  1:4443265/1‑140 (MQ=18)                                                   ccACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                         >  1:38411/1‑140 (MQ=18)                                                   ccACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                         >  1:3559370/1‑140 (MQ=18)                                                   ccACCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgt                         >  1:3558416/1‑140 (MQ=18)                                                     aCCGCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATGGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGTAACTCATAGTTAACAGTGTAGGTCACGACGAAAGTGGGCACTACGCCGAtgttgt                       >  2:2406674/1‑140 (MQ=11)                                                     aCCGCTAGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGgtgc                       <  2:4937892/140‑2 (MQ=16)                                                      ccGCTGGCAGCGTCGATGTGCTTGTCGATGTCGATTCCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTAGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGtt                      <  2:581542/140‑1 (MQ=255)                                                        gCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGtttt                    <  2:4691463/140‑1 (MQ=18)                                                        gCTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGtttt                    <  1:4023139/140‑1 (MQ=18)                                                         cTGGCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTc                   >  1:2974137/1‑140 (MQ=18)                                                            gCAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAACGTCGGCACCACGCCGATGGTGTTTTCGTc                >  2:1538943/1‑140 (MQ=16)                                                             cAGCGTCGATGTGTATGTCGATGTCGATACCGGTATCGGCCTGGACTTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACCCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:4415226/140‑1 (MQ=255)                                                             cAGCGTCGATGTGGATGTCGATGTCGATACCGGTATCGGCCTGGATTTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATACTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:4415265/140‑1 (MQ=255)                                                             cAGCGTCGATGTGCCTGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               >  2:2401915/1‑140 (MQ=18)                                                             cAGCGTCGATGTGCATGTCGATGTCGATCCCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:1122207/140‑1 (MQ=12)                                                             cAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:38411/140‑1 (MQ=18)                                                             cAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:36182/140‑1 (MQ=18)                                                             cAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:4964457/140‑1 (MQ=18)                                                             cAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:1815581/140‑1 (MQ=18)                                                             cAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:5019316/140‑1 (MQ=18)                                                             cAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               >  2:3299702/1‑140 (MQ=14)                                                             cAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:2662136/140‑1 (MQ=18)                                                             cAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:2662028/140‑1 (MQ=18)                                                             cAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:2261647/140‑1 (MQ=18)                                                             cAGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:1506115/140‑1 (MQ=18)                                                             cAGCGTCGATGTGCATGTCGATGTAGATACAGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTca               <  2:399596/140‑1 (MQ=12)                                                              aGCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTcac              <  1:1836497/140‑1 (MQ=18)                                                               gCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTcaca             <  2:318222/140‑1 (MQ=18)                                                               gCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTcaca             <  1:4275989/140‑1 (MQ=18)                                                               gCGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCAAGCGCATCGTCCAGCGGTTGTGGGAACTCCTAGTTACCAGCGTAGGTCACGCCGAAAGTCCGCACCACGCCGATGGTGTTTTCGTcaca             <  2:3872531/140‑1 (MQ=11)                                                                cGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATGCAGCGCATCGTGCAGCGGTTGTGGGCACTCATAGTTACCAGTGTAGGTGACGCCGAAAGTCGGCACCACGCCGACGGTGTTTTCGTCACAg            >  1:4290164/1‑140 (MQ=11)                                                                cGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAg            >  1:2928975/1‑140 (MQ=18)                                                                cGTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAg            >  1:2928873/1‑140 (MQ=18)                                                                 gTCGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAgg           >  1:1771175/1‑140 (MQ=18)                                                                   cGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCt         >  2:2017705/1‑140 (MQ=18)                                                                   cGATGTGCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCt         >  1:3068184/1‑140 (MQ=18)                                                                        tgCATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAAt    >  1:1658132/1‑140 (MQ=18)                                                                          cATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAATCa  >  1:1130472/1‑140 (MQ=18)                                                                          cATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAATCa  >  1:3025246/1‑140 (MQ=18)                                                                          cATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAATCa  <  2:3113857/140‑1 (MQ=18)                                                                          cATGTCGATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAATCa  >  1:1130559/1‑140 (MQ=18)                                                                                |                                                                                                                                      GCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCTATGTCGATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTCATAGTTACCAGTGTAGGTCACGCCGAAAGTCGGCACCACGCCGATGGTGTTTTCGTCACAGGCTTCAATCA  >  NZ_CP025268/1576072‑1576284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑