Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 159,854 G→A F20F (TTC→TTT)  gluQRS ← tRNA glutamyl‑Q(34) synthetase GluQRS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268159,8540GA99.4% 1270.7 / NA 340F20F (TTC→TTT) gluQRStRNA glutamyl‑Q(34) synthetase GluQRS
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base A (132/205);  total (134/206)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 340 total aligned reads displayed.

TCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCA  >  NZ_CP025268/159720‑159986
                                                                                                                                      |                                                                                                                                    
tCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATg                                                                                                                                 >  1:2999047/1‑140 (MQ=255)
tCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATg                                                                                                                                 >  1:2186486/1‑140 (MQ=255)
   aGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAg                                                                                                                              <  2:2237593/140‑1 (MQ=255)
    gCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGc                                                                                                                             >  2:2155220/1‑140 (MQ=255)
    ccTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGc                                                                                                                             <  2:4703415/139‑1 (MQ=255)
      tGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTc                                                                                                                           <  1:432176/140‑1 (MQ=255)
        gcgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGc                                                                                                                         <  2:1705734/140‑1 (MQ=255)
        gcgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGc                                                                                                                         <  2:1859663/140‑1 (MQ=255)
        gcacAGGTTTGTTTCAGCGGCACCGGGGGCTTCACGAGGCGGGTCGATCTCTTCTATACGTACCAGCCGGCGACCTTGCCGGGCGCGAGCCTGCAACTAGCTGCCGAGCGCGGCGATCAACGAGCCAAAATGAAGCTCGc                                                                                                                         <  2:599892/137‑1 (MQ=255)
         cgcAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGCTATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGcc                                                                                                                        >  2:2759973/1‑140 (MQ=255)
            aGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGa                                                                                                                     <  2:3250584/140‑1 (MQ=255)
               aTAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAgg                                                                                                                  <  2:4642400/140‑1 (MQ=255)
                  gTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGgaga                                                                                                               <  1:1428137/140‑1 (MQ=255)
                        gCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGc                                                                                                         >  1:1964841/1‑140 (MQ=255)
                        gCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGc                                                                                                         >  1:965803/1‑140 (MQ=255)
                          ggCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGa                                                                                                       >  2:3089123/1‑140 (MQ=255)
                           gCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGaa                                                                                                      <  1:3468966/140‑1 (MQ=255)
                             cccGGGAACTTCACGAGGGGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:4802130/139‑1 (MQ=255)
                             cccGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:1220422/139‑1 (MQ=255)
                             cccGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:3981754/139‑1 (MQ=255)
                             cccGGGAACCTCACCAGTCGGGTAGCTATCTTCTATGCGTCCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGCGGGGGCGAAGc                                                                                                    <  2:244814/139‑1 (MQ=255)
                             actgggAGCTTCACGAGGCGGGTGGGTATCTTCTAGGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCGGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:1775728/137‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:3949540/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:2094833/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:5071937/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:3307527/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:3544368/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  2:2661776/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:3394199/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:3105081/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:2155220/140‑1 (MQ=255)
                             aCCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    >  1:1061875/1‑140 (MQ=255)
                             aCCGGGAAATTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:756933/140‑1 (MQ=255)
                             aCCCGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGc                                                                                                    <  1:2367430/140‑1 (MQ=255)
                               ggggAACTTCACGAGGCGCGTCCATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGACGAGCGCGGCGACCAGAGAGCCAAACTGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  <  2:4434568/139‑1 (MQ=255)
                               cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  <  2:2311143/140‑1 (MQ=255)
                               cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  <  1:4141763/140‑1 (MQ=255)
                               cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  <  2:1001074/140‑1 (MQ=255)
                               cGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCgg                                                                                                  <  1:1172941/140‑1 (MQ=255)
                                gggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGc                                                                                                 <  2:136173/140‑1 (MQ=255)
                                gggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGc                                                                                                 <  2:54616/140‑1 (MQ=255)
                                 ggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGcc                                                                                                <  2:5010842/140‑1 (MQ=255)
                                 ggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGcc                                                                                                >  1:475134/1‑140 (MQ=255)
                                 ggAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGcc                                                                                                <  2:3455944/140‑1 (MQ=255)
                                      ttCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAatat                                                                                           >  1:1751101/1‑140 (MQ=255)
                                      ttCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAatat                                                                                           >  2:4260566/1‑140 (MQ=255)
                                         aCGAGGCGGGTCGATATCTTCTATGCGTACCGGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACt                                                                                        <  1:3861449/140‑1 (MQ=255)
                                         aCGAGGCGGGTCGATATCTTCTAGGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACt                                                                                        <  2:4054574/140‑1 (MQ=255)
                                            aGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACtgtg                                                                                     <  2:1519505/140‑1 (MQ=255)
                                            aGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACtgtg                                                                                     <  2:2767983/140‑1 (MQ=255)
                                             ggCGAGTCGATATCTTCTATGCTTACCAGCCAGCGACCTTGCCGTGCGCGCGCCTGCGAATAGCTCCCGAGCGCGGCGATCAGAGAGACAAAATGAACATCGCCGTAAGGAGAGGGGGCGAAGCTGCAAATATGAtgtga                                                                                    >  2:3589235/1‑139 (MQ=255)
                                              gCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACtgtgtg                                                                                   <  2:1915110/140‑1 (MQ=255)
                                                gggTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGtgtc                                                                                 >  1:681583/1‑140 (MQ=255)
                                                  gTCGATATCTTCTATGCGTACCAGCCAACGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCAAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCAAATATACTATGtgtctg                                                                               >  2:3737062/1‑140 (MQ=255)
                                                           ttctATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCtt                                                                      >  2:3185590/1‑140 (MQ=255)
                                                            tctATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCttt                                                                     >  2:2613216/1‑140 (MQ=255)
                                                            tctATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCttt                                                                     >  2:724180/1‑140 (MQ=255)
                                                            tctATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCttt                                                                     >  1:4380405/1‑140 (MQ=255)
                                                              tATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGa                                                                   >  2:2981520/1‑140 (MQ=255)
                                                                tGCGTACCAGCCAGCGACCTTGCCGGGCGCGCGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATGTACTGTGTGTCTGTCATCTCTTTGAAc                                                                 >  1:4093733/1‑140 (MQ=255)
                                                                tGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAAc                                                                 >  1:4945131/1‑140 (MQ=255)
                                                                tGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAAc                                                                 <  1:3708983/140‑1 (MQ=255)
                                                                 gCGTACCAGCCAGCGACCTTGCCGGGAGCGAGACTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACa                                                                <  2:332931/140‑1 (MQ=255)
                                                                 ccGTACCAGCCAGCGCCCTTGCAGGGCGAGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACa                                                                <  2:4364354/139‑1 (MQ=255)
                                                                  cGTACTAGCCCGCGCCCTTGCCGGGCGCCAGCCTGCAAATAGCTGCCGAGCGCGGGGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGCGAGGGCGCGAAGCGGCCAATATACTGTGTGCCTGTCATCTCTTTGAACaa                                                               >  2:2399842/1‑140 (MQ=255)
                                                                    tACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaa                                                             >  2:806643/1‑140 (MQ=255)
                                                                     aCCAGTCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                            >  2:1449534/1‑140 (MQ=255)
                                                                     aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                            >  1:3791451/1‑140 (MQ=255)
                                                                     aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                            >  1:4029614/1‑140 (MQ=255)
                                                                     aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                            >  1:4574459/1‑140 (MQ=255)
                                                                     aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                            >  1:1616209/1‑140 (MQ=255)
                                                                     aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                            >  1:101704/1‑140 (MQ=255)
                                                                     aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                            >  1:1495855/1‑140 (MQ=255)
                                                                     aCCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACaaaaa                                                            >  1:482665/1‑140 (MQ=255)
                                                                       cagccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAAt                                                          <  1:240635/140‑1 (MQ=255)
                                                                        agccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATa                                                         >  2:1930218/1‑140 (MQ=255)
                                                                          ccagcGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGACAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGTGGCCAATATACTGTGTGTCTGTCATCTCTTTCAACAAAAAATAAg                                                       >  2:4407048/1‑140 (MQ=255)
                                                                              cGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCgg                                                   >  1:2738472/1‑140 (MQ=255)
                                                                                 ccTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAg                                                <  1:4930268/140‑1 (MQ=255)
                                                                                 ccTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGAGCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGACCAAAAAATAAGGCGGGAg                                                <  2:4159636/140‑1 (MQ=255)
                                                                                 ccTTGCCGGGCGCGAGCCTGCAAATAACAGCCGAGCGGGGCGAGCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGGGTGTCTGTCATCTCTGTGAACAAAAAATAAGGCGGGAg                                                <  2:244557/140‑1 (MQ=255)
                                                                                   ttGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCa                                              <  1:5094907/140‑1 (MQ=255)
                                                                                    tGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCAt                                             >  1:130084/1‑140 (MQ=255)
                                                                                          gcgcGAGCCTGCAAATAGCTGCCGAGCGCGGAGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCg                                       >  2:3887115/1‑140 (MQ=255)
                                                                                            gcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                     <  1:5079329/140‑1 (MQ=255)
                                                                                            gcgAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGcc                                     <  1:3600691/140‑1 (MQ=255)
                                                                                               aGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCtgt                                  >  1:26526/1‑140 (MQ=255)
                                                                                               aGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCtgt                                  >  1:3443830/1‑140 (MQ=255)
                                                                                                  ccccAAATAGCTCCCGCGCGCGGCGATCAGAGCGCAAACATGCAGCTCGCCGGAACGAGAGGGGCCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGt                               <  2:2891945/137‑1 (MQ=255)
                                                                                                   tGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTa                              >  2:382461/1‑140 (MQ=255)
                                                                                                     cgaaTAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTaaa                            <  2:137885/138‑1 (MQ=255)
                                                                                                     cAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTaaa                            <  1:2009835/140‑1 (MQ=255)
                                                                                                      aaaTAGCTGACGAGCGCGGCGATCAGAGAGCGAAAATGAAGCCGCCCGCAAGGTGAGGGGGCGATGCGGACAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATACGGCGGGAGGATTTCCCGCCTGTGGTAAAc                           <  2:1633426/140‑1 (MQ=255)
                                                                                                            cTGCCGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATg                     >  2:4772851/1‑140 (MQ=255)
                                                                                                               ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                  >  1:2317231/1‑140 (MQ=255)
                                                                                                               ccGAGCGCGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGaa                  <  1:2858419/140‑1 (MQ=255)
                                                                                                                   gcgcGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGc              <  2:4956271/140‑1 (MQ=255)
                                                                                                                   gcgcGGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGc              <  1:91131/140‑1 (MQ=255)
                                                                                                                    cgcgGCGATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCt             >  2:1149269/1‑140 (MQ=255)
                                                                                                                         cgATCAGAGAGCCAAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAAt        >  2:825672/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                    
TCCAGCTGGCGCAGGATAGTTTCTGCGGCACCGGGAACTTCACGAGGCGGGTCGATATCTTCTATGCGTACCAGCCAGCGACCTTGCCGGGCGCGAGCCTGCAAATAGCTGCCGAGCGCGGCGATCAGAGAGCCGAAATGAAGCTCGCCGGAAGGAGAGGGGGCGAAGCGGCCAATATACTGTGTGTCTGTCATCTCTTTGAACAAAAAATAAGGCGGGAGCATTTCCCGCCTGTGGTAAACGTGATGGAACGGCTGTAATTAGCCA  >  NZ_CP025268/159720‑159986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑