BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,603,437	T→C	531 (TAA→TAG)	lsrK ←	autoinducer‑2 kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,603,437	0	T	C	99.5%	664.2 / NA	199	531 (TAA→TAG)	lsrK	autoinducer‑2 kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (131/66); minor base A (1/0); total (133/66)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 199 total aligned reads displayed.

TGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGC  >  NZ_CP025268/1603302‑1603569

tGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACtata                                                                                                                                  >  1:765670/1‑140 (MQ=255)

         ttcttgggATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCTGCTCTAAAAGAAGGGAAATAAGAATGACTATACTCAAAAACTAACATCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGgcg                                                                                                                         <  2:146077/133‑1 (MQ=255)

         tACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGgcg                                                                                                                         >  1:79670/1‑140 (MQ=255)

           cTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCt                                                                                                                       <  1:1653561/140‑1 (MQ=255)

           cTGGTGATGGAACGATGAATCTGCAAGCTGACTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCt                                                                                                                       <  2:1506831/140‑1 (MQ=255)

            tGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGTAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCtt                                                                                                                      >  2:3043894/1‑140 (MQ=255)

            tGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCtt                                                                                                                      >  1:832716/1‑140 (MQ=255)

            tGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCtt                                                                                                                      >  2:332432/1‑140 (MQ=255)

            tGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCtt                                                                                                                      >  2:2768517/1‑140 (MQ=255)

              gTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTc                                                                                                                    >  2:4333971/1‑140 (MQ=255)

              gTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTc                                                                                                                    >  2:24846/1‑140 (MQ=255)

                 aTGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCAt                                                                                                                 >  1:5014651/1‑140 (MQ=255)

                 aTGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCAt                                                                                                                 >  1:2858865/1‑140 (MQ=255)

                 aTGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCAt                                                                                                                 >  2:2845956/1‑140 (MQ=255)

                  tGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATa                                                                                                                <  1:198703/140‑1 (MQ=255)

                   tgAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGGAATAAGAATAACGATAATCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATaa                                                                                                               >  2:2558200/2‑140 (MQ=255)

                   ggAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAGTAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATaa                                                                                                               >  1:87799/1‑140 (MQ=255)

                   ggAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTACCAGCCACGGTCATCATGATGTGGCTGTCAATGAAAATATAACCCAGGCGCTTTCCATaa                                                                                                               >  2:4910924/1‑140 (MQ=255)

                   ggAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATaa                                                                                                               >  2:2624834/1‑140 (MQ=255)

                   ggAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATaa                                                                                                               >  2:1482135/1‑140 (MQ=255)

                        gATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacgacg                                                                                                          >  1:289276/1‑140 (MQ=255)

                        gATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacgacg                                                                                                          >  1:4328899/1‑140 (MQ=255)

                        gATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAacgacg                                                                                                          >  2:4397344/1‑140 (MQ=255)

                            aaTCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                      <  1:258105/140‑1 (MQ=255)

                            aaTCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                      >  1:2356951/1‑140 (MQ=255)

                            aaTCTGCAAGCTGGCTGTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGAcgtcgt                                                                                                      <  1:1721909/140‑1 (MQ=255)

                              tCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCa                                                                                                    >  1:1001120/1‑140 (MQ=255)

                                tGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGt                                                                                                  <  1:2701705/140‑1 (MQ=255)

                                tGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGt                                                                                                  >  2:62489/1‑140 (MQ=255)

                                tGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACACCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGt                                                                                                  <  2:2683986/140‑1 (MQ=255)

                                          tatttAACAAGCCAGCTCTAAAAGAAGGAAAATAAGAATAACTACACTCAAAAACTAACAGCCACGTTCATCATGATGTGGCTGTCAATGAAACTATAACCTAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCaa                                                                                        <  2:90763/138‑1 (MQ=255)

                                            tttAACAAGCCACCTCTCAAAGAACGGAAATAAGCATAACTATACCCAAACACTAACAGCCACGCTCATCATGATGTGGCTGTCAATGAACCTCTAACCCAGGCGCTTTCCATAACGACGTCCTCAGTCCATGATCAAcc                                                                                      <  2:4620202/140‑1 (MQ=255)

                                                        gCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGa                                                                          <  2:4642021/140‑1 (MQ=255)

                                                         ctctAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGAt                                                                         >  2:4179144/1‑140 (MQ=255)

                                                         ctctAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGAt                                                                         >  1:1251552/1‑140 (MQ=255)

                                                              aaaGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATGACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGa                                                                    >  1:4506975/1‑140 (MQ=255)

                                                              aaaGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGa                                                                    >  1:1775387/1‑140 (MQ=255)

                                                                  aagGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATaaa                                                                <  2:1110884/140‑1 (MQ=255)

                                                                  aagGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATaaa                                                                <  2:4834607/140‑1 (MQ=255)

                                                                   agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAc                                                               >  1:4176265/1‑140 (MQ=255)

                                                                   agGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAc                                                               >  1:1461934/1‑140 (MQ=255)

                                                                   agGGAAATAAGAATAACTAAAATCAAAAACTAACAGCCACGGTAATCATGATGTGGCTGTCAATGAAACGATAACCCAGGCGCATTCCAAAACGAAGTCGTCAATCCATAATCAACCAGCCCCAGCTGATACTGATAAAc                                                               >  2:3670913/1‑140 (MQ=255)

                                                                    gggAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAct                                                              >  1:2038563/1‑140 (MQ=255)

                                                                    gggAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAAct                                                              >  1:2608083/1‑140 (MQ=255)

                                                                      gAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCCTGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgc                                                            <  2:2610372/140‑1 (MQ=255)

                                                                      gAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgc                                                            <  2:3721146/140‑1 (MQ=255)

                                                                         aTAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAActgcctg                                                         >  1:1936157/1‑140 (MQ=255)

                                                                                 aaCTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTAt                                                 <  1:3325960/140‑1 (MQ=255)

                                                                                     agaatCAAAAACTAACAGCCACGCTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATcgcg                                             <  2:4261996/136‑1 (MQ=255)

                                                                                      taCTCAAAAACTCACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCCGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGt                                            <  2:565067/140‑1 (MQ=255)

                                                                                       aCTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTg                                           <  1:3984459/140‑1 (MQ=255)

                                                                                        cTCAAAAACTAACAGCGACGGTCATCATGATGTGGCTGTCAATGAAACTATAATCCACGCGCTTACCATAGAGACGTCGTCAGTCGATGATCAACCAGCTCCAGCTGATCCTGATGAACTGCCTGCCACTGATCGGGTgg                                          >  2:798090/1‑139 (MQ=255)

                                                                                        cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGa                                          >  2:3589014/1‑140 (MQ=255)

                                                                                        cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGa                                          >  1:37928/1‑140 (MQ=255)

                                                                                        cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGa                                          >  1:3317122/1‑140 (MQ=255)

                                                                                        cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGa                                          >  1:5073852/1‑140 (MQ=255)

                                                                                        cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGa                                          >  1:3038641/1‑140 (MQ=255)

                                                                                        cTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGa                                          >  1:3559017/1‑140 (MQ=255)

                                                                                           aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCATCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATc                                       >  1:3021556/1‑140 (MQ=255)

                                                                                           aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCGCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTCAATc                                       >  1:4648023/1‑140 (MQ=255)

                                                                                           aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATc                                       >  1:2506487/1‑140 (MQ=255)

                                                                                           aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATc                                       >  2:2649507/1‑140 (MQ=255)

                                                                                           aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATc                                       >  1:2442522/1‑140 (MQ=255)

                                                                                           aaaaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATc                                       >  1:566693/1‑140 (MQ=255)

                                                                                             aaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCt                                     >  2:524806/1‑140 (MQ=255)

                                                                                             aaaCTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACCATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCt                                     >  2:4672606/1‑140 (MQ=255)

                                                                                                 tAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATa                                 <  2:2277960/140‑1 (MQ=255)

                                                                                                 tAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATa                                 <  2:2464584/140‑1 (MQ=255)

                                                                                                  aaCAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGTTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATaa                                >  2:4251617/1‑140 (MQ=255)

                                                                                                   aCAGCCACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATaaa                               >  1:4801466/1‑140 (MQ=255)

                                                                                                       ccACGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTc                           >  2:3895523/1‑140 (MQ=255)

                                                                                                          cGGTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATg                        <  1:3709826/140‑1 (MQ=255)

                                                                                                           ggTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGc                       >  1:4712894/1‑140 (MQ=255)

                                                                                                           ggTCATCATGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGc                       >  1:1039375/1‑140 (MQ=255)

                                                                                                                tcatGATGTGGCTGTCAATGAACCTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                  <  1:3857021/140‑1 (MQ=255)

                                                                                                                tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                  <  1:4289106/140‑1 (MQ=255)

                                                                                                                tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                  <  1:632184/140‑1 (MQ=255)

                                                                                                                tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                  <  1:3108112/140‑1 (MQ=255)

                                                                                                                tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                  <  1:2160402/140‑1 (MQ=255)

                                                                                                                tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                  <  2:4762461/140‑1 (MQ=255)

                                                                                                                tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                  <  1:2200940/140‑1 (MQ=255)

                                                                                                                tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                  <  2:2037277/140‑1 (MQ=255)

                                                                                                                tcatGATGTGGCTGTCAATGAAACTATAACCCAGGCGCGTTCCATAACGACGTCGTCAGGCCATGATCAACCAGCCCCAGCTGACCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTc                  <  1:3862454/140‑1 (MQ=255)

                                                                                                                 catGATGTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATACACTGCCTGCCACTTCTCGCGTGAATCCTGATAAAGTTCATGCTTTTcc                 >  2:4441911/1‑140 (MQ=255)

                                                                                                                     atgTGGCTGTCAATGAACCCATAACCCAGGCGCTTTCCATAACGCCGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAACGTTCATGCTTTTCCCGGt             >  2:3409741/1‑140 (MQ=255)

                                                                                                                     atgTGGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGt             >  2:225850/1‑140 (MQ=255)

                                                                                                                       gtgGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCGGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCt           >  2:4703241/1‑140 (MQ=255)

                                                                                                                       gtgGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCt           >  2:69998/1‑140 (MQ=255)

                                                                                                                       gtgGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCt           >  1:2735278/1‑140 (MQ=255)

                                                                                                                       gtgGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCt           >  1:3000170/1‑140 (MQ=255)

                                                                                                                        tgGCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTg          <  1:2625130/140‑1 (MQ=255)

                                                                                                                         ggCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTgg         >  2:100696/1‑140 (MQ=255)

                                                                                                                          gCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCGGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTggg        >  2:2834562/1‑139 (MQ=255)

                                                                                                                          gCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCATCCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgt        <  1:2845956/140‑1 (MQ=255)

                                                                                                                          gCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgt        <  2:4847642/140‑1 (MQ=255)

                                                                                                                          gCTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgt        <  2:1821509/140‑1 (MQ=255)

                                                                                                                           cTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTGTCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtg       >  2:2927597/1‑140 (MQ=255)

                                                                                                                           cTGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtg       >  1:3879921/1‑140 (MQ=255)

                                                                                                                            tGTCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtgt      >  1:4421563/1‑140 (MQ=255)

                                                                                                                              tCAATGAAACTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGgtgtgt    >  1:190224/1‑140 (MQ=255)

TGAATATCGTACTGGTGATGGAACGATGAATCTGCAAGCTGGCTTTTAACAAGCCAGCTCTAAAAGAAGGGAAATAAGAATAACTATACTCAAAAACTAACAGCCACGGTCATCATGATGTGGCTGTCAATGAAATTATAACCCAGGCGCTTTCCATAACGACGTCGTCAGTCCATGATCAACCAGCCCCAGCTGATCCTGATAAACTGCCTGCCACTTATCGCGTGAATCCTGATAAAGTTCATGCTTTTCCGGGTCTGGTGTGTGC  >  NZ_CP025268/1603302‑1603569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`