Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,643,849 T→C *166* (TAA→TAG)  rzpQ ← DUF2514 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,643,8490TC98.9% 294.2 / NA 91*166* (TAA→TAG) rzpQDUF2514 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (67/23);  minor base G (1/0);  total (68/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAATAAAACGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGTGAGCATTC  >  NZ_CP025268/1643718‑1643985
                                                                                                                                   |                                                                                                                                        
taataaAACGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTg                                                                                                                                  >  1:4259207/1‑140 (MQ=255)
taataaAACGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTg                                                                                                                                  >  2:214617/1‑140 (MQ=255)
taataaAACGTGGCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGGGTCCTTTTTATTTAAAGTTTACATTCCGGGATGTAAATGTTCGGAGTTAAGGTTACCGGACATTTg                                                                                                                                  >  2:2895086/1‑140 (MQ=255)
 aataaAACGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGa                                                                                                                                 <  1:3148624/140‑1 (MQ=255)
     aaaCGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAGATGTTCCGATTTAATATTACCCTACATTTGATggt                                                                                                                             >  2:4131876/1‑138 (MQ=255)
     aaaCGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCt                                                                                                                             >  1:3416870/1‑140 (MQ=255)
          tgtCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTa                                                                                                                        >  2:594660/1‑140 (MQ=255)
          tgtCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTa                                                                                                                        <  1:278750/140‑1 (MQ=255)
           gtCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTGTTATTTAAATTTTACATGCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTAt                                                                                                                       <  1:2495574/140‑1 (MQ=255)
            tCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGAGATGTAAATGTTCCGATTTAATATTACCCTACATTTGTTGCTTTTTAtt                                                                                                                      >  2:4080736/1‑139 (MQ=255)
             cATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATGTAATATTACCCTACATTTGATGCTTTTTAtct                                                                                                                     >  1:429972/1‑140 (MQ=255)
                tCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCtt                                                                                                                  >  1:3719473/1‑140 (MQ=255)
                    atgaaGACCTTTTATCTTACCGTTAAGATTTCAGGAATTTTGGCTCATGGTAGAATCCTTTTTGTTTAAATTTTCGATTCCCCGATGTAAATGTTCCGATTTAATATGAGCCTACATTTGGTGCTTTTTATCTCTTaaac                                                                                                              >  2:3248517/4‑139 (MQ=255)
                    aTTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAg                                                                                                              >  1:314732/1‑140 (MQ=255)
                    aTTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAg                                                                                                              <  1:4902944/140‑1 (MQ=255)
                    aTTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAg                                                                                                              <  1:2476073/140‑1 (MQ=255)
                    aTTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAg                                                                                                              <  1:2474172/140‑1 (MQ=255)
                    aTTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGACCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTATTATTACCCTACATTTGATGCTTTTTATCTCTTAAAg                                                                                                              >  2:3779124/1‑140 (MQ=255)
                      tAAGACCTTTTATCTTACCCTTAAGATGTCAGGAATTTTGGCTCATTGTAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTGTTTATCTCTTAAAGAt                                                                                                            >  2:112352/1‑140 (MQ=255)
                         gACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCa                                                                                                         >  1:2691675/1‑140 (MQ=255)
                              tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGCTGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGAt                                                                                                    <  1:4038114/140‑1 (MQ=255)
                              tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGAt                                                                                                    >  1:4826554/1‑140 (MQ=255)
                              tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGAt                                                                                                    >  1:2446965/1‑140 (MQ=255)
                              tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGAt                                                                                                    >  2:3087142/1‑140 (MQ=255)
                              tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGAt                                                                                                    <  2:1096485/140‑1 (MQ=255)
                              tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGAt                                                                                                    >  2:382241/1‑140 (MQ=255)
                              tttATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGAt                                                                                                    >  1:4081477/1‑140 (MQ=255)
                              tttATCTTACCCTTAAGATGTCAGGAATTTTGGCTGATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGGGATGTACATGGTCCGATTTAATATGACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATacag                                                                                                    >  2:1635917/1‑137 (MQ=255)
                              tttATCTTACCCTTAAGATCTCAGGAACTTTCGCTCATGGAAGAGTCCATTTTATTTAGATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTCTCTCTTAAAGATTCATAGAt                                                                                                    >  2:392472/1‑140 (MQ=255)
                                     tagccTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTGAATTTTACATTCCGCGATGTAAATGGTCCGATTTAATATTAGCCTACATTTGATGCTGTTTATCTCTTAAAGATTCATAGATCTGTTgg                                                                                             >  2:1885580/4‑139 (MQ=255)
                                      aCCCTTAAGATTTCAGGAATTTTTGCTCATGGAAGAGTCGTTTTTATTTAAATTTTACATTCCGAGATGTAAATGTTACGATTTAGTATTACCCTACATTTGATGCTTTTTATATCTTAAAGATTCATAGATATGTTGAc                                                                                            >  2:3869759/1‑140 (MQ=255)
                                      aCCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGAc                                                                                            >  2:4676433/1‑140 (MQ=255)
                                      aCCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGAc                                                                                            >  1:3150144/1‑140 (MQ=255)
                                       cccTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACa                                                                                           >  1:4400679/1‑140 (MQ=255)
                                       cccTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACa                                                                                           >  1:4907690/1‑140 (MQ=255)
                                           tAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTc                                                                                       <  1:1255454/140‑1 (MQ=255)
                                            aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTTTTAAAGATTCATAGATCTGTTGACAAGTCa                                                                                      >  2:870115/1‑140 (MQ=255)
                                            aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCa                                                                                      >  1:2119919/1‑140 (MQ=255)
                                            aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCa                                                                                      >  1:5043866/1‑140 (MQ=255)
                                            aaGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCa                                                                                      >  1:1902999/1‑140 (MQ=255)
                                             aGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCAc                                                                                     <  1:1297929/140‑1 (MQ=255)
                                             aGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCAc                                                                                     <  2:5057895/140‑1 (MQ=255)
                                                   cAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGc                                                                               >  1:1517502/1‑140 (MQ=255)
                                                   cAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGc                                                                               >  1:1517119/1‑140 (MQ=255)
                                                   cAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGc                                                                               >  1:202365/1‑140 (MQ=255)
                                                    aGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTTACTCCTGCg                                                                              >  2:1075854/1‑140 (MQ=255)
                                                                tCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCg                                                                  <  1:2880313/140‑1 (MQ=255)
                                                                   tGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCa                                                               >  2:3669955/1‑140 (MQ=255)
                                                                   tGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCa                                                               >  2:3670259/1‑140 (MQ=255)
                                                                   tGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCa                                                               >  2:3670413/1‑140 (MQ=255)
                                                                         agTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAAtt                                                         >  1:2099188/1‑140 (MQ=255)
                                                                         agTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAAtt                                                         >  1:4491679/1‑140 (MQ=255)
                                                                           tCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTc                                                       >  1:4988427/1‑140 (MQ=255)
                                                                               ttttATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCa                                                   <  1:637822/140‑1 (MQ=255)
                                                                               tttaATTTCAATTTTACATTCCCCGATGTAAATGTTCCGATTTAATATTACCCTACATTTAATGCTTTTTATCACTTAAAGATTCAAAGACCTGTTGACAACTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCa                                                   <  2:149403/140‑1 (MQ=255)
                                                                                tttatttaAATTTTACATTCCGCGATGTAGATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATGGATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCAt                                                  >  2:2278979/1‑140 (MQ=255)
                                                                                tttatttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCAt                                                  >  1:1596185/1‑140 (MQ=255)
                                                                                tttatttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCAt                                                  >  2:3489067/1‑140 (MQ=255)
                                                                                tttatttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCAt                                                  >  1:4182707/1‑140 (MQ=255)
                                                                                tttatttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCAt                                                  >  2:3749595/1‑140 (MQ=255)
                                                                                  tatttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATaa                                                >  1:3305613/1‑140 (MQ=255)
                                                                                    tttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAg                                              >  1:910055/1‑140 (MQ=255)
                                                                                     ttaAATTTTACATTCCGCGCTGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGt                                                                                        >  2:3827524/1‑97 (MQ=255)
                                                                                     ttaAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGt                                                                                        <  1:3827524/97‑1 (MQ=255)
                                                                                          ttttACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCCTAAAGCTGAGc                                        <  2:2057635/140‑1 (MQ=255)
                                                                                             tACATTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCCCTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTc                                     <  2:1563817/140‑1 (MQ=255)
                                                                                                aTTCCGCGATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTctgc                                  <  2:2121830/140‑1 (MQ=255)
                                                                                                    cgcgATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCa                              <  1:2074711/140‑1 (MQ=255)
                                                                                                    cgcgATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCa                              >  2:2682278/1‑140 (MQ=255)
                                                                                                    cgcgATGTAAATGGTCCGATTTAATATTATCCTACCTTTGATGCTTGTTATCTCTTAAAGAGTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGGTAGCAATGGGATGGTAAAGCTGATCTTCTGCTGCa                              >  2:1133633/1‑140 (MQ=255)
                                                                                                      cgATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAat                            >  1:216742/1‑140 (MQ=255)
                                                                                                      cgATGTAAATGTTCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAat                            <  2:138729/140‑1 (MQ=255)
                                                                                                                 ttCCGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCa                 >  2:576402/1‑140 (MQ=255)
                                                                                                                 ttCCGATTTAATATTACCCTACATTTGATGCGTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCCCTCCTGCGATGTACCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCa                 <  2:4115584/140‑1 (MQ=255)
                                                                                                                   ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACCAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg               >  1:3739001/1‑140 (MQ=255)
                                                                                                                   ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCTATATCTCCGAGCATg               >  2:1400108/1‑140 (MQ=255)
                                                                                                                   ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCACTATCTCCGAGCATg               >  2:4462478/1‑140 (MQ=255)
                                                                                                                   ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg               >  2:505194/1‑140 (MQ=255)
                                                                                                                   ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg               >  1:1097724/1‑140 (MQ=255)
                                                                                                                   ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg               >  1:2122937/1‑140 (MQ=255)
                                                                                                                   ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg               >  2:3427087/1‑140 (MQ=255)
                                                                                                                   ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg               >  2:1247546/1‑140 (MQ=255)
                                                                                                                   ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATg               >  1:4377116/1‑140 (MQ=255)
                                                                                                                   ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCATTCGTCAGCAATTTCAACATAAAGCGGAGCTTGTGCTGCAATATCTCCGAGCATg               >  2:2306365/1‑140 (MQ=255)
                                                                                                                   ccGATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTAACTCATGGGATGTAGCGTTCGTCAGCAATTTCAGCATATAGTTGAGCTTCTGCTGCAATATCTCCGAGCATg               >  2:4543072/1‑140 (MQ=255)
                                                                                                                     gATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGtt             >  2:408466/1‑140 (MQ=255)
                                                                                                                     gATTTAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGtt             >  1:4031938/1‑140 (MQ=255)
                                                                                                                         tAATATTACCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGTg         <  2:1994790/140‑1 (MQ=255)
                                                                                                                              ttACCCTACATTCGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGTGAGCAt    <  2:574868/140‑1 (MQ=255)
                                                                                                                                aCCCTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGTGAGCATTc  >  1:2441388/1‑140 (MQ=255)
                                                                                                                                aCCCTACATTTGATGCATTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTATGCTGCAATATCTCAGAGCATGTTGGTGAGCATTc  >  2:4272551/1‑140 (MQ=255)
                                                                                                                                   |                                                                                                                                        
TAATAAAACGTGTCATTCTGATTAAGACCTTTTATCTTACCCTTAAGATTTCAGGAATTTTGGCTCATGGAAGAGTCCTTTTTATTTAAATTTTACATTCCGCGATGTAAATGTTCCGATTTAATATTACCTTACATTTGATGCTTTTTATCTCTTAAAGATTCATAGATCTGTTGACAAGTCACTCCTGCGATGTAGCGTTCGTCAGCAATTTCAGCATAAAGCTGAGCTTCTGCTGCAATATCTCCGAGCATGTTGGTGAGCATTC  >  NZ_CP025268/1643718‑1643985

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑