BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	14,302	T→C	V45A (GTG→GCG)	dnaJ →	molecular chaperone DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	14,302	0	T	C	99.6%	842.3 / NA	257	V45A (GTG→GCG)	dnaJ	molecular chaperone DnaJ
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (135/119); total (136/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

ATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACT  >  NZ_CP025268/14169‑14436

aTGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAtt                                                                                                                                  >  1:4861184/1‑140 (MQ=255)

    cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                              <  2:3852252/140‑1 (MQ=255)

    cTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTaaa                                                                                                                              <  1:2088269/140‑1 (MQ=255)

    cTAAGCAAGATTATTACGACATTTTAGGCGTTTACCAACCAGCGGAAGAACGTGAAATCAGAAAGGCCTAAAAACGCCTGGCCATGAAATCCCAACCGGAACGTAACCAGAGTGCAAAAGAGGCCGAGGCGTAATTTaaa                                                                                                                              <  1:506668/140‑1 (MQ=255)

         cAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGAt                                                                                                                         >  1:3197928/1‑140 (MQ=255)

               tattaCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGa                                                                                                                   <  1:1892427/140‑1 (MQ=255)

                  taCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGc                                                                                                                <  2:3659087/140‑1 (MQ=255)

                      agaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTAt                                                                                                            <  2:3516051/140‑1 (MQ=255)

                      agaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTAt                                                                                                            >  1:3709770/1‑140 (MQ=255)

                      agaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTAt                                                                                                            <  1:1773198/140‑1 (MQ=255)

                       gaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                           <  1:1122482/140‑1 (MQ=255)

                       gaTGTTAGTCGTTTCTAAAACAGCGGAAGAGCGTGAAACCAGAAAGGCGTACAAACGCCTGGCCATGAAATACCACCTGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                           <  2:3587075/140‑1 (MQ=255)

                       gaTGTTAGGCGTTACGAAAACAGCGGAAGAGCGTGAAATGAGAAAGGCATACAAACCCCTGGCCATGAAATACCACCCCGACCGTAACCAGCGTGACACAGAGGCCGAGGCGAAATTTAAAGGGATCAAGGAAGCTTATg                                                                                                           <  2:2720512/140‑1 (MQ=255)

                        aTTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGa                                                                                                          >  2:3885390/1‑140 (MQ=255)

                                  tttCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGAcc                                                                                                >  1:671474/1‑140 (MQ=255)

                                             gCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaa                                                                                     >  1:539903/1‑140 (MQ=255)

                                              cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                    >  1:1766665/1‑140 (MQ=255)

                                              cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                    >  1:4462624/1‑140 (MQ=255)

                                              cGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCaaaaa                                                                                    >  1:335353/1‑140 (MQ=255)

                                              cGGAAGAGCGTGAAATCAGAAAGGCAGACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAACTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGCCCGACTCGCaaaaa                                                                                    >  2:3225032/1‑140 (MQ=255)

                                                   gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCCCCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                               <  2:2060034/140‑1 (MQ=255)

                                                   gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAACCGTGc                                                                               <  2:3071150/140‑1 (MQ=255)

                                                   gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                               <  1:3490946/140‑1 (MQ=255)

                                                   gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                               <  1:1096328/140‑1 (MQ=255)

                                                   gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                               <  1:2111832/140‑1 (MQ=255)

                                                   gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                               <  1:1684254/140‑1 (MQ=255)

                                                   gagCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGAACAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGc                                                                               <  2:1033895/140‑1 (MQ=255)

                                                      cGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGc                                                                            >  1:103385/1‑140 (MQ=255)

                                                       gtaaaaTCAGAACGGCCAACAAACGCCTGGCAATGAAATACACCCCGGACCGTAACCAGGGCGAAAAAGAGGCAGAGGCGAAATTTAAAGAGATCACGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCa                                                                           <  2:2644777/137‑1 (MQ=255)

                                                       gTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCa                                                                           <  1:2901884/140‑1 (MQ=255)

                                                        tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGCGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                          <  1:3307398/140‑1 (MQ=255)

                                                        tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                          >  1:1666267/1‑140 (MQ=255)

                                                        tGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCAt                                                                          <  1:1913174/140‑1 (MQ=255)

                                                          aaaGCAGAAAGGTCTACACACTCCTGGCCATGAAATACCCCCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTCAAAGAGATCGAGGAAGCTTATGAAGTTCTGACCGACGCGCAAAAACGTGCGGCATAc                                                                        <  2:407163/140‑1 (MQ=255)

                                                           aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACTAGGAGGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTGAGGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                       <  1:3632682/140‑1 (MQ=255)

                                                           aaTCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACg                                                                       <  1:366268/140‑1 (MQ=255)

                                                             tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCCCCCCGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAACTTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                     <  1:290920/140‑1 (MQ=255)

                                                             tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGCTCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                     <  1:2715411/140‑1 (MQ=255)

                                                             tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                     <  1:2505827/140‑1 (MQ=255)

                                                             tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                     <  1:3382849/140‑1 (MQ=255)

                                                             tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                     <  1:178288/140‑1 (MQ=255)

                                                             tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                     <  1:3022181/140‑1 (MQ=255)

                                                             tCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGAt                                                                     <  1:386764/140‑1 (MQ=255)

                                                             tCAGAAACGCCTACACCCCCCTGGCCATGATATCCCACCCGGACCGTAACAAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGACCAAGGCCGCTTATGCAGTTCTGACCGACTCGCAAAATCGTGCGCCATACGAt                                                                     <  2:1790913/140‑1 (MQ=255)

                                                              cAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATc                                                                    <  1:1330460/140‑1 (MQ=255)

                                                                aaaaGGCCTACAAACGCATGGCCATGAAATACCACCCGGACCGTAACCCGGGTGACAAAAAGGCCGAGGCGAAATTTAAAGAGCTCAAGGAAGCTTATGAAGTTCTGTCCGACTCGCAAAAACGTGCGGCATACGATCAg                                                                  <  1:2186519/139‑1 (MQ=255)

                                                                 aaaGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGt                                                                 >  1:180083/1‑140 (MQ=255)

                                                                  aaGGCCTACAATCGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTa                                                                <  2:4643794/140‑1 (MQ=255)

                                                                  aaGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAGGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTa                                                                <  1:927369/140‑1 (MQ=255)

                                                                        tACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCa                                                          <  1:5100239/140‑1 (MQ=255)

                                                                         aCAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAt                                                         >  1:1866649/1‑140 (MQ=255)

                                                                           aaaCGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtgc                                                       <  2:915707/140‑1 (MQ=255)

                                                                            aaCGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtgct                                                      <  1:3511476/140‑1 (MQ=255)

                                                                                ccTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGt                                                  <  1:2130379/140‑1 (MQ=255)

                                                                                    gCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGa                                              >  2:4306792/1‑140 (MQ=255)

                                                                                    gCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGa                                              >  1:951447/1‑140 (MQ=255)

                                                                                     ccATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGCCCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAg                                             <  2:3850009/140‑1 (MQ=255)

                                                                                      cATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGc                                            <  1:4170615/140‑1 (MQ=255)

                                                                                        tGAAATACCACCCGGACCGTAACTAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCGGACTGACTTGCAAAAACGTGCGGCATACGATCAGTATGGTGATTCTGCGTTTGAGCaa                                          >  1:1266963/1‑140 (MQ=255)

                                                                                        tGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCaa                                          <  1:3588990/140‑1 (MQ=255)

                                                                                         gAAATACCCCACGGAACGTAAACAGAGTGACAAAGAGGCAGAGACGAAATTTAAGGAGATCAAGGAAGCTTATGAATTTCTTACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                         <  2:4713189/140‑1 (MQ=255)

                                                                                         gAAATACCACCCGTACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                         <  2:4380358/140‑1 (MQ=255)

                                                                                         gAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAg                                         <  2:936818/140‑1 (MQ=255)

                                                                                              accaccCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATGTAAAGAGATCAAGGAAGCGTATGAAGTACTGACCGACTCCCAAAAGCGTGCGGCATACGATCAGTATGGTCATGCAGCGTTTGAGCAAGGAGGc                                    >  2:5006632/1‑140 (MQ=255)

                                                                                                  cccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATgg                                >  1:3432901/1‑140 (MQ=255)

                                                                                                   ccGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATGTAAAGAGATCAAGGAAGCGTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTAGGGTCATGATGCGTCTGAGCAAGGTGGCATggg                               >  2:2452426/1‑140 (MQ=255)

                                                                                                      gACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcgg                            >  2:4164793/1‑140 (MQ=255)

                                                                                                      gACCGTAACCAGGGTCACAAAGAGGCCGAGGCGACATTTAAAGATATCAAGGAACCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCATTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcgg                            <  2:616228/140‑1 (MQ=255)

                                                                                                       aCCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggc                           >  1:305347/1‑140 (MQ=255)

                                                                                                       aCCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggc                           >  1:473148/1‑140 (MQ=255)

                                                                                                       aCCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATAAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCAGGggcggc                           >  1:768270/1‑140 (MQ=255)

                                                                                                         cGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcgg                         <  1:728236/140‑1 (MQ=255)

                                                                                                         cGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcgg                         >  2:4502893/1‑140 (MQ=255)

                                                                                                          gTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcggc                        <  2:2946116/140‑1 (MQ=255)

                                                                                                          gTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGggcggcggc                        <  2:2191548/140‑1 (MQ=255)

                                                                                                           tAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGgcggcggcg                       >  1:4619676/1‑140 (MQ=255)

                                                                                                           tAACCAGGGGGACAAAGAGGCCGAGGCGAACTTTAAAGAGATCAAGGAAGCTTCTGAAGTGCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAGGCTGCGTTTGAGCAAGGGGGCATGGgcggcggcg                       >  2:858126/1‑140 (MQ=255)

                                                                                                                 gggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTg                 >  2:2077187/1‑140 (MQ=255)

                                                                                                                 gggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTg                 >  2:3750458/1‑140 (MQ=255)

                                                                                                                 gggTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTGTGAGCAAGGTGGCATGGGCGGCGGCGGTtgtg                 >  2:188931/1‑140 (MQ=255)

                                                                                                                 gggTGACAAAGAGGCCGAGGCGAAATTGAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTg                 >  2:3352142/1‑140 (MQ=255)

                                                                                                                    tGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcg              <  2:520276/140‑1 (MQ=255)

                                                                                                                      aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc            >  1:1494977/1‑140 (MQ=255)

                                                                                                                      aCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggc            >  2:4941092/1‑140 (MQ=255)

                                                                                                                       cagagagGAAGAGGCGAAATTTAAAGAGATCAAGTAGGCATATGACGTTCTGACCGAGTCGGAGAAACGTGCGGCAAACGATCAATATGGTCGTGCTGCGTTTGAGCAAGGTTACATGGGCGGCGGCAGGCGTggcggcg           >  2:1195269/4‑140 (MQ=255)

                                                                                                                       cAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCGGTAGGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggcg           >  1:3628298/1‑140 (MQ=255)

                                                                                                                       cAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCTTTTGAGCAAGGTGGCAAGGGCGGCGGCGGTTTTggcggcg           >  1:4675571/1‑140 (MQ=255)

                                                                                                                        aaaGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTggcggcgg          >  1:3757179/1‑140 (MQ=255)

                                                                                                                            agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa      >  1:1208377/1‑140 (MQ=255)

                                                                                                                            agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa      <  2:3097521/140‑1 (MQ=255)

                                                                                                                            agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa      >  1:634244/1‑140 (MQ=255)

                                                                                                                            agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa      >  2:2137704/1‑140 (MQ=255)

                                                                                                                            agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa      >  1:2648839/1‑140 (MQ=255)

                                                                                                                            agGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCCTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCa      <  1:4347846/140‑1 (MQ=255)

                                                                                                                              gacGAGGCGAAAATTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGGATGGTCATGCTGCGGTTGAGCAAGGGGGCATGGGGGGGGGCGGTTGTGGCGGCGGCGCAGa    >  2:1469897/3‑140 (MQ=255)

                                                                                                                              gCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGa    >  1:4579002/1‑140 (MQ=255)

                                                                                                                              gCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGa    >  1:2311535/1‑140 (MQ=255)

                                                                                                                              gCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGa    >  2:1382840/1‑140 (MQ=255)

                                                                                                                                cGAGGCGCAATTTAAAGAGATCAAGGACGCTTATGAAGTTCTGCCCGACTCGCAAAACCGTGCGGCATACGATCAGTATGGTCCTGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGGTTTGGCGGCGGCGAAGACt  >  2:1731913/1‑140 (MQ=255)

                                                                                                                                cGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACt  >  1:1549879/1‑140 (MQ=255)

ATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGTGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACT  >  NZ_CP025268/14169‑14436

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`