Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,797,084	T→C	*155* (TAA→TAG)	nlpC ←	NlpC/P60 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,797,084	0	T	C	100.0%	293.7 / NA	92	*155* (TAA→TAG)	nlpC	NlpC/P60 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (54/38);  total (54/38)

GTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGT  >  NZ_CP025268/1796958‑1797219                                                                                                                               |                                                                                                                                        gTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCg                                                                                                                            >  1:2139180/1‑140 (MQ=255)  tCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGc                                                                                                                           <  1:3933653/140‑1 (MQ=255)                       aTTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGAATCGTCTCGCCTGCCACCCATTTTTCTACCa                                                                                                      >  1:3528131/1‑140 (MQ=255)                         tataAACGAATGATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAAt                                                                                                    <  1:1239613/140‑1 (MQ=255)                         tataAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAAt                                                                                                    <  1:1460459/140‑1 (MQ=255)                            aaaCGAATAATCCATTTCCGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCAGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGACATTTTTCTGCCAATAGa                                                                                                 >  2:2187713/1‑140 (MQ=255)                            aaaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTCAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCCATAGa                                                                                                 >  2:2186066/1‑140 (MQ=255)                            aaaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGa                                                                                                 >  2:1083498/1‑140 (MQ=255)                            aaaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGa                                                                                                 >  1:2006460/1‑140 (MQ=255)                             aaCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGAc                                                                                                >  2:1576549/1‑140 (MQ=255)                              aCGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCTTCGTCCAGTTGTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACa                                                                                               <  1:1714008/140‑1 (MQ=255)                                gAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACAtt                                                                                             <  1:4304141/140‑1 (MQ=255)                                 aataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAATTTTTTCTGCCAATTGAAATTa                                                                                            >  1:3806980/1‑140 (MQ=255)                                 aataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTa                                                                                            >  2:786760/1‑140 (MQ=255)                                  ataatCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTAt                                                                                           <  1:618451/140‑1 (MQ=255)                                          tttACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAg                                                                                   <  1:4803088/140‑1 (MQ=255)                                           ttACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAAGGGACTGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAgc                                                                                  >  2:378488/1‑139 (MQ=255)                                             aCGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGaa                                                                                <  1:5038714/140‑1 (MQ=255)                                                gAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGCCGGCGACGTATCATCGCCGGACATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGCAATCTTTCTGCCAATAGACATTATCAAGTGAGGAACGc                                                                             >  2:713914/1‑140 (MQ=255)                                                 aaTTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAACTTTTTCTGCCAATAGACATTATCCCGTGAGGAACGCa                                                                            >  1:4625997/1‑140 (MQ=255)                                                  aTTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATAAAGTGAGGAACGCAt                                                                           <  1:653318/140‑1 (MQ=255)                                                      ttGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACt                                                                       <  2:2633688/140‑1 (MQ=255)                                                         tCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCTTCCATTTTTCGACATACTAGATTCGTCTCTCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGTAACGCATCACTccc                                                                    >  2:627622/1‑140 (MQ=255)                                                         tCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTccc                                                                    <  1:3433738/140‑1 (MQ=255)                                                         tCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTccc                                                                    <  1:3433760/140‑1 (MQ=255)                                                         tCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTccc                                                                    <  1:4534857/140‑1 (MQ=255)                                                             cAAAATACTACTGTATTCAGGAGTAAATGTACGGCGATGTATCATCGCCGTCCCTTTTTCGACATACTAGATTCGTCTCTCCTGCCAGAAATTTTTCTTCCAATAGACAATATCAAGTGAGGAACGCCTCACTCCCATGc                                                                >  1:1005642/1‑140 (MQ=255)                                                             cAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGc                                                                <  1:1176020/140‑1 (MQ=255)                                                                  tactacTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTa                                                           <  1:5077605/140‑1 (MQ=255)                                                                  tactacTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTGTCGGCATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTa                                                           <  1:3624810/140‑1 (MQ=255)                                                                     tactGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGTCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAATGCATCACTCCCTTGCTGGTggag                                                        >  2:3021699/1‑140 (MQ=255)                                                                       ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCa                                                                         >  2:841242/1‑121 (MQ=255)                                                                       ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCa                                                                         <  1:841242/121‑1 (MQ=255)                                                                       ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCa                                                                         <  1:841241/121‑1 (MQ=255)                                                                       ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCa                                                                         >  2:841241/1‑121 (MQ=255)                                                                       ctGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGc                                                      <  1:3707005/140‑1 (MQ=255)                                                                         gTATTCAGTAGTAAATGGACGCCGATGTATCATCGCCGTCCAGTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGt                                                    <  1:3159247/140‑1 (MQ=255)                                                                          tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTg                                                   >  2:3776704/1‑140 (MQ=255)                                                                          tATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTg                                                   >  2:2713293/1‑140 (MQ=255)                                                                            ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGa                                                 <  1:330098/140‑1 (MQ=255)                                                                            ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGa                                                 >  2:4458796/1‑140 (MQ=255)                                                                            ttCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGa                                                 >  2:3431886/1‑140 (MQ=255)                                                                               aGGAGTAAATGGTCGGCGATGTATCATCGCCGTCAATTTTTCGACGAACGAGAGGCGTCTAGGCTGCCAGAAATTGTTCTGCCAATAGACATTATCAAGTGAAGAACGCATCACTACCCTGCTGGTAGAGGCGTGGATaa                                              >  2:5038453/1‑140 (MQ=255)                                                                               aGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATaa                                              >  2:804064/1‑140 (MQ=255)                                                                               aGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATaa                                              >  2:1583120/1‑140 (MQ=255)                                                                               aGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATaa                                              >  2:111170/1‑140 (MQ=255)                                                                                 gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGATGCGTGGATAAAt                                            >  2:2441921/1‑140 (MQ=255)                                                                                 gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAt                                            >  2:4060306/1‑140 (MQ=255)                                                                                 gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAt                                            <  1:4882227/140‑1 (MQ=255)                                                                                 gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAt                                            >  2:4955023/1‑140 (MQ=255)                                                                                 gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAAt                                            >  2:1846579/1‑140 (MQ=255)                                                                                 gAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTAGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCACTTGCTTGTAGAGGCGTGGATAAAt                                            >  2:2485076/1‑140 (MQ=255)                                                                                    tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGCCATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTgg                                         >  2:4441055/1‑140 (MQ=255)                                                                                    tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAg                                                                                   >  2:4868934/1‑98 (MQ=255)                                                                                    tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAg                                                                                   <  1:4868934/98‑1 (MQ=255)                                                                                    tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACt                                                                       >  2:1848331/1‑110 (MQ=255)                                                                                    tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACt                                                                       <  1:1848331/110‑1 (MQ=255)                                                                                    tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTgg                                         >  2:746478/1‑140 (MQ=255)                                                                                    tAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTgg                                         <  1:326568/140‑1 (MQ=255)                                                                                     aaaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGCACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgt                                        <  1:4995552/140‑1 (MQ=255)                                                                                      aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCTCTTGCTGGTAGAGGCGTGGATAAATTGgtt                                       >  2:4736098/1‑140 (MQ=255)                                                                                      aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgtt                                       >  2:95464/1‑140 (MQ=255)                                                                                      aaTGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgtt                                       >  2:3165769/1‑140 (MQ=255)                                                                                       aTGAACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGgttg                                      <  1:1749464/140‑1 (MQ=255)                                                                                          gaaggCGATGTATCATCGCCGTCCATATTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGttgttg                                   <  1:4319547/137‑1 (MQ=255)                                                                                          gACGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGttgttg                                   <  2:2006460/140‑1 (MQ=255)                                                                                          gACGGCGATGTATCATCGCCGTACATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATCGACATTATAAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGttgttg                                   <  1:1810274/140‑1 (MQ=255)                                                                                           aCGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGGTGTTgg                                  >  2:3562011/1‑140 (MQ=255)                                                                                            cGGTGATGTATTATCGCCGTCCATTCTTCGACATCCTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGt                                 <  1:3853330/140‑1 (MQ=255)                                                                                            cGGCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGt                                 <  1:839273/140‑1 (MQ=255)                                                                                             ggCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTa                                <  1:2443493/140‑1 (MQ=255)                                                                                              gCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTAt                               >  2:4654403/1‑140 (MQ=255)                                                                                              gCGATGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGGTGATAAATTGGTTGTTGGTAt                               >  2:305557/1‑140 (MQ=255)                                                                                                  tGTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTa                           <  1:141470/140‑1 (MQ=255)                                                                                                  tGTATCATCGCCGTCCATTTTTCGACATACTAGATGCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCAATCCCTTGCAGGTAGAGGCGTGGATAAATTAGTTGTTGGTGTTGTa                           >  2:1269700/1‑140 (MQ=255)                                                                                                   gTATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGCGGAACGCATCACTGCCTTGCTGGTAGCGGCGTGGATAAATTGGTTGTTGGTATCGTaa                          >  2:4215819/1‑140 (MQ=255)                                                                                                    tATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaaa                         <  1:654331/140‑1 (MQ=255)                                                                                                    tATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaaa                         >  2:1921707/1‑140 (MQ=255)                                                                                                    tATCATCGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTaaa                         <  1:4155806/140‑1 (MQ=255)                                                                                                       catcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAAAGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATAc                      >  2:2354443/1‑140 (MQ=255)                                                                                                         tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGTACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt                    >  2:4149169/1‑140 (MQ=255)                                                                                                         tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATGACTCCCTTGCTGGTAGGGGCGTGGATCAGGTGGTTGTTGGTATCGTagagaact                    >  2:3915992/1‑133 (MQ=255)                                                                                                         tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTTGTATCGTAAATACCt                    >  2:418596/1‑140 (MQ=255)                                                                                                         tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt                    >  2:3444474/1‑140 (MQ=255)                                                                                                         tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt                    >  2:3444569/1‑140 (MQ=255)                                                                                                         tcGCCGTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCt                    >  2:1630954/1‑140 (MQ=255)                                                                                                              gTCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGGAATTTTTCTGCCAATAGACAGTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTATAGGCGTGGATAAATTGGTTGTTGGTATGGTAAATACCTACATg               >  2:1863847/1‑140 (MQ=255)                                                                                                               tCCATTTTTCGAGATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACGTACATGc              >  2:5039510/1‑140 (MQ=255)                                                                                                               tCCATTTTTCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGc              >  2:445578/1‑140 (MQ=255)                                                                                                                       tCGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCAtt      <  1:263911/140‑1 (MQ=255)                                                                                                                       ccGACATACTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGCACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCAtt      <  1:263717/139‑1 (MQ=255)                                                                                                                           cATACTAGATTCGTCTCGCCTGCCAGCAATTTTTCTGCCAGTAGACACTATCCAGAGAGGGACGCATCCCTCACTTGCTGGTGGAGGCGGGGATAACTTGGTTGTTGGTATGGGAAATACCGCCATGAAAACCAtttcaa  >  2:3815778/1‑137 (MQ=255)                                                                                                                               |                                                                                                                                        GTCCTTTTTTTAGATTATCCTGATTATAAACGAATAATCCATTTACGGAATTTTTGTCTGCAAAATACTACTGTATTCAGGAGTAAATGGACGGCGATGTATCATCGCCGTCCATTTTTCGACATATTAGATTCGTCTCGCCTGCCAGAAATTTTTCTGCCAATAGACATTATCAAGTGAGGAACGCATCACTCCCTTGCTGGTAGAGGCGTGGATAAATTGGTTGTTGGTATCGTAAATACCTACATGCAAACCATTTTGT  >  NZ_CP025268/1796958‑1797219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑