BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,808,384	A→G	pseudogene (474/1900 nt)	espL1 →	type III secretion system effector EspL1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,808,384	0	A	G	100.0%	953.9 / NA	284	pseudogene (474/1900 nt)	espL1	type III secretion system effector EspL1
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (214/70); total (214/70)

Only 100 of 284 total aligned reads displayed.

ACCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAATGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACA  >  NZ_CP025268/1808251‑1808519

aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCagg                                                                                                                                   >  2:2860639/1‑138 (MQ=255)

aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                   >  1:133192/1‑140 (MQ=255)

aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                   >  2:4343793/1‑140 (MQ=255)

aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                   >  1:4756936/1‑140 (MQ=255)

aCCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGAGCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATg                                                                                                                                   >  1:700540/1‑140 (MQ=255)

        cGAAGCATACTGGATTATATTGAACAAGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTTTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTgg                                                                                                                           >  2:4637218/1‑139 (MQ=255)

            gCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAAtc                                                                                                                       >  1:3942776/1‑140 (MQ=255)

             cATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAAtct                                                                                                                      <  1:2040396/140‑1 (MQ=255)

                     aTTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGa                                                                                                              >  1:1972693/1‑140 (MQ=255)

                       tataTTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACg                                                                                                            >  2:256531/1‑140 (MQ=255)

                       tataTTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTGTTTTGCAATCTCCTTCTGACg                                                                                                            >  2:262675/1‑140 (MQ=255)

                         tatTGAACGGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGGGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCGTTCTGACGCt                                                                                                          >  2:4368710/1‑140 (MQ=255)

                         tatTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCt                                                                                                          >  1:2362184/1‑140 (MQ=255)

                         tatTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCt                                                                                                          >  2:3775033/1‑140 (MQ=255)

                              aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                     >  2:584765/1‑140 (MQ=255)

                              aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                     >  2:4193994/1‑140 (MQ=255)

                              aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                     >  1:1700764/1‑140 (MQ=255)

                              aacaggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttt                                                                                                     >  1:4392256/1‑140 (MQ=255)

                                caggaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTAtttttt                                                                                                   >  1:336250/1‑140 (MQ=255)

                                   gaacagTTAACTTCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                                >  2:5052332/1‑140 (MQ=255)

                                   gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTGAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                                >  2:4074758/1‑140 (MQ=255)

                                   gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                                >  1:2189734/1‑140 (MQ=255)

                                   gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                                >  2:2806084/1‑140 (MQ=255)

                                   gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                                >  2:3256256/1‑140 (MQ=255)

                                   gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                                >  1:3120925/1‑140 (MQ=255)

                                   gaacagTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGATAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTc                                                                                                >  2:3765293/1‑140 (MQ=255)

                                       agTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCa                                                                                            <  1:3455823/140‑1 (MQ=255)

                                        gTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCAt                                                                                           >  1:1588434/1‑140 (MQ=255)

                                        gTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCAt                                                                                           >  1:3464023/1‑140 (MQ=255)

                                        gTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAAAGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGccct                                                                                           >  2:3013865/1‑138 (MQ=255)

                                            aCTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGt                                                                                       <  1:1056041/140‑1 (MQ=255)

                                             ctgctgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAACAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTc                                                                                      >  2:3985690/1‑140 (MQ=255)

                                             ctgctgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTc                                                                                      >  1:4869177/1‑140 (MQ=255)

                                             ctgctgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTc                                                                                      >  1:1453831/1‑140 (MQ=255)

                                              tgctgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCa                                                                                     >  2:1222875/1‑140 (MQ=255)

                                              tgctgAAGATCGTAACAATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGGGCATGTTTTTTGCAATCTCCTTCTGACGCTCTTTTTGGTCGCCATCTGGCa                                                                                     >  2:3136986/1‑140 (MQ=255)

                                               gctgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCAt                                                                                    <  2:4391413/140‑1 (MQ=255)

                                                 tgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTg                                                                                  >  1:3347807/1‑140 (MQ=255)

                                                 tgAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTg                                                                                  >  1:2709710/1‑140 (MQ=255)

                                                   aaGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCa                                                                                                                          >  2:2493301/1‑98 (MQ=255)

                                                   aaGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCa                                                                                >  2:3041242/1‑140 (MQ=255)

                                                      aTCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATa                                                                             <  1:573589/140‑1 (MQ=255)

                                                        cGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACg                                                                           >  2:202490/1‑140 (MQ=255)

                                                          tAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGcc                                                                         >  2:3648255/1‑140 (MQ=255)

                                                             ccATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCtt                                                                      >  1:1472355/1‑140 (MQ=255)

                                                                ttGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGAt                                                                   <  2:2186544/140‑1 (MQ=255)

                                                                 tGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGAtt                                                                  >  1:1852842/1‑140 (MQ=255)

                                                                 tGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGAtt                                                                  >  1:772114/1‑140 (MQ=255)

                                                                 tGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGAtt                                                                  >  1:3051783/1‑140 (MQ=255)

                                                                 tGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGAtt                                                                  >  2:308110/1‑140 (MQ=255)

                                                                   tATGAAACTGTCTAAAAAAATCCATAGAGACAAAAACTATACAAACAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATATCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCa                                                                >  2:648863/1‑140 (MQ=255)

                                                                     tGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCAtt                                                              >  1:1921472/1‑140 (MQ=255)

                                                                        aaCTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTgag                                                           >  2:2157515/1‑140 (MQ=255)

                                                                        aaCTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTgag                                                           >  2:3093473/1‑140 (MQ=255)

                                                                        aaCTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATAAGCCCTTGATTCATTgag                                                           >  1:4975810/1‑140 (MQ=255)

                                                                          cTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAAGCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTATGTCGCCATCTGTCATTACAATACGCCCTTGATTCATTGAGaa                                                         >  1:4645921/1‑140 (MQ=255)

                                                                           tGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGaaa                                                        >  2:4948718/1‑140 (MQ=255)

                                                                             tCTAAAAAAATCCATAGAGAAAAAACCTATACACCAACCAGTAAATCTCAAGGGTAGTGCATGTTTTTTGCAATCTCCTTTTGACGCGATTTATTGTTGCCATCTGTCATTGCAAAACGACCTTGATTCATTGAGAAATg                                                      >  2:737285/1‑140 (MQ=255)

                                                                             tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                      >  2:2665466/1‑140 (MQ=255)

                                                                             tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                      >  1:2087475/1‑140 (MQ=255)

                                                                             tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                      >  2:1942371/1‑140 (MQ=255)

                                                                             tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                      >  1:3852235/1‑140 (MQ=255)

                                                                             tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                      >  1:2613041/1‑140 (MQ=255)

                                                                             tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                      >  1:2687331/1‑140 (MQ=255)

                                                                             tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                      >  2:4271799/1‑140 (MQ=255)

                                                                             tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                      >  1:5061540/1‑140 (MQ=255)

                                                                             tCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATg                                                      >  2:4856969/1‑140 (MQ=255)

                                                                               tAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGa                                                    >  2:1719928/1‑140 (MQ=255)

                                                                               tAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGGCATTGCAATACGCCCTTGATTCATTGAGAAATGGa                                                    <  2:5033918/140‑1 (MQ=255)

                                                                                aaaaaaaTCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGaa                                                   <  2:169136/140‑1 (MQ=255)

                                                                                    aaaTCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAgg                                               <  2:5101366/140‑1 (MQ=255)

                                                                                    aaaTCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTGCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAgg                                               <  2:974413/140‑1 (MQ=255)

                                                                                       tCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCaa                                            >  2:455975/1‑140 (MQ=255)

                                                                                           tAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTc                                        >  1:1282561/1‑140 (MQ=255)

                                                                                              agaAAAAAACTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAAc                                     <  1:2562765/140‑1 (MQ=255)

                                                                                                  aaaaaCTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGTCGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGa                                 >  1:1520500/1‑140 (MQ=255)

                                                                                                  aaaaaCTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGa                                 <  2:1371268/140‑1 (MQ=255)

                                                                                                   aaaaCTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGAt                                >  1:3846347/1‑140 (MQ=255)

                                                                                                       cTATACACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTaaa                            <  2:1798674/140‑1 (MQ=255)

                                                                                                          taCACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACTCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACAt                         >  1:3189107/1‑140 (MQ=255)

                                                                                                          taCACCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGAGTAAACAt                         >  1:4124422/1‑140 (MQ=255)

                                                                                                            cacCCAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTa                       <  1:44760/140‑1 (MQ=255)

                                                                                                                cAACAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcc                   <  2:1466556/140‑1 (MQ=255)

                                                                                                                 aaCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcct                  >  1:3722426/1‑140 (MQ=255)

                                                                                                                 aaCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcct                  >  1:1003273/1‑140 (MQ=255)

                                                                                                                 aaCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcct                  <  2:3573549/140‑1 (MQ=255)

                                                                                                                 aaCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGAATCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTActcct                  >  2:4092300/1‑140 (MQ=255)

                                                                                                                  aCAGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGCACTGGAAAAGGCAAAGTCAACCTGATTAAACATTActcctc                 >  1:4731836/1‑140 (MQ=255)

                                                                                                                    aGTAAATCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCg               >  2:767510/1‑140 (MQ=255)

                                                                                                                     gTAAACCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGt              <  2:3953997/140‑1 (MQ=255)

                                                                                                                       aaaTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCGTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTg            >  1:2857868/1‑140 (MQ=255)

                                                                                                                         aTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGaa          >  2:1406761/1‑140 (MQ=255)

                                                                                                                         aTCTCAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGaa          >  2:2046875/1‑140 (MQ=255)

                                                                                                                          tctcAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAAt         <  2:3937891/140‑1 (MQ=255)

                                                                                                                           ctcAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATc        >  2:2935000/1‑140 (MQ=255)

                                                                                                                           ctcAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATc        >  2:1533333/1‑140 (MQ=255)

                                                                                                                           ctcAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATc        >  2:332777/1‑140 (MQ=255)

                                                                                                                             cAATGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCa      <  2:3850052/140‑1 (MQ=255)

                                                                                                                              aaTGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCAt     >  2:2765433/1‑140 (MQ=255)

                                                                                                                                tGGTAGTGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATAc   >  1:3229529/1‑140 (MQ=255)

ACCATTTGCGAAGCATACTGGATTATATTGAACAGGAACAGTTAACTGCTGAAGATCGTAACCATTGTATGAAACTGTCTAAAAAAATCCATAGAGAAAAAAACTATACACCCAACAGTAAATCTCAATGGTAATGCATGTTTTTTGCAATCTCCTTCTGACGCTATTTTTTGTCGCCATCTGTCATTGCAATACGCCCTTGATTCATTGAGAAATGGAAAAGGCAAAGTCAACCTGATTAAACATTACTCCTCCGTTGAATCCATACA  >  NZ_CP025268/1808251‑1808519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`