BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	183,450	A→G	K386R (AAG→AGG)	cdaR →	DNA‑binding transcriptional regulator CdaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	183,450	0	A	G	100.0%	1034.4 / NA	317	K386R (AAG→AGG)	cdaR	DNA‑binding transcriptional regulator CdaR
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (186/131); total (186/131)

Only 100 of 317 total aligned reads displayed.

CAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCT  >  NZ_CP025268/183315‑183585

cAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGtt                                                                                                                                      <  2:1950588/140‑1 (MQ=255)

aaaaGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGtt                                                                                                                                      <  2:109627/139‑1 (MQ=255)

 aaaGGCGTTGTTTATTCATCGTCATCCCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTa                                                                                                                                     <  2:76032/140‑1 (MQ=255)

 aaaGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTa                                                                                                                                     <  2:2048349/140‑1 (MQ=255)

      cGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGt                                                                                                                                <  1:308728/140‑1 (MQ=255)

       gttgttTATTCATCGTAATACCCTCGAGTATCGGCTTAATCGTATCTCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGtt                                                                                                                               <  2:2449521/140‑1 (MQ=255)

        ttgttTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTa                                                                                                                              >  2:1558975/1‑140 (MQ=255)

           tttATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAgg                                                                                                                           >  1:915504/1‑140 (MQ=255)

             tATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGtt                                                                                                                         <  2:609769/140‑1 (MQ=255)

                tCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAg                                                                                                                      <  2:4347857/140‑1 (MQ=255)

                 cATCGTAATACCCTGGAGTATCGGCTTAATCGTCTATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACCACT‑GGATGAAGAGCGGTAGGTTATGCGTTCAGGTTGAgg                                                                                                                     <  1:1352012/140‑1 (MQ=255)

                 cATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAgg                                                                                                                     <  2:2241918/140‑1 (MQ=255)

                 cATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAgg                                                                                                                     <  2:4981021/140‑1 (MQ=255)

                 cATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAgg                                                                                                                     <  1:818110/140‑1 (MQ=255)

                 cATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAgg                                                                                                                     <  1:4662565/140‑1 (MQ=255)

                     gTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAggcggc                                                                                                                 >  2:3166834/1‑140 (MQ=255)

                     gTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAggcggc                                                                                                                 >  1:3988460/1‑140 (MQ=255)

                     gTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAggcggc                                                                                                                 >  1:4751688/1‑140 (MQ=255)

                       aaTACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGgcgc                                                                                                               <  1:3820835/140‑1 (MQ=255)

                              tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGCGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                                        >  1:4687052/1‑140 (MQ=255)

                              tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                                        >  1:1417533/1‑140 (MQ=255)

                              tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                                        >  1:216115/1‑140 (MQ=255)

                              tGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                                        <  2:1670703/140‑1 (MQ=255)

                              tGGAGTATCGGCCTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCa                                                                                                        >  2:5028320/1‑140 (MQ=255)

                               ggaggaTCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGACAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTATGGTTGAGGCGGCGCTGGCTCAt                                                                                                       >  2:2827776/1‑140 (MQ=255)

                               ggAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAt                                                                                                       <  1:4128412/140‑1 (MQ=255)

                               ggAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAt                                                                                                       <  1:1963963/140‑1 (MQ=255)

                                gAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTGGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAtt                                                                                                      >  1:5033830/1‑140 (MQ=255)

                                gAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAtt                                                                                                      >  1:2773528/1‑140 (MQ=255)

                                gAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCAtt                                                                                                      >  2:371458/1‑140 (MQ=255)

                                     tCGGCTTAATCGTATATCGGAACTGACCGGGGTGGATTTGGGCAATTGTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCGCATTCGCCt                                                                                                 >  2:581778/1‑140 (MQ=255)

                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGtt                                                                                                                                      <  2:4682682/103‑1 (MQ=255)

                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  1:612095/1‑140 (MQ=255)

                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  1:560273/1‑140 (MQ=255)

                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  1:2395796/1‑140 (MQ=255)

                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  1:2577560/1‑140 (MQ=255)

                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  1:2884185/1‑140 (MQ=255)

                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 <  1:4336129/140‑1 (MQ=255)

                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGG‑GGCGCTGGCTCCATCCCCTc                                                                                                >  1:3728705/1‑140 (MQ=255)

                                     tCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAAGTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCt                                                                                                 >  2:3263816/1‑140 (MQ=255)

                                      cGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTc                                                                                                <  2:1065068/140‑1 (MQ=255)

                                      cGGCTTAATCGTATAGCGGAACTGAACGGGCTTGAGTTGGGCAATTTTGATGAAAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCGCATTCCCCTc                                                                                                >  2:3209968/1‑140 (MQ=255)

                                           tAATCGTATATCGGAACTTACCGGGCTTGATGTTGGCAATTTTGATGACAGGTGGCTGCTGTATGTGGGGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCGCCTCAGccc                                                                                           >  2:4509819/1‑140 (MQ=255)

                                             aTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCg                                                                                          >  1:1281444/1‑139 (MQ=255)

                                             aTCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGa                                                                                         >  2:2518806/1‑140 (MQ=255)

                                              tCGTATCTCGGAACTGTCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACCACT‑GGAGGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCgca                                                                                        <  2:1820866/140‑3 (MQ=255)

                                              tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  1:4835422/140‑1 (MQ=255)

                                              tCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTAAAACT‑GGATGAAGAGCAGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGaa                                                                                        <  2:1210672/140‑1 (MQ=255)

                                                  atatCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGGTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGt                                                                                    >  2:1720755/1‑140 (MQ=255)

                                                   tatCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTa                                                                                   <  2:2903458/140‑1 (MQ=255)

                                                   tatCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTa                                                                                   >  2:2375242/1‑140 (MQ=255)

                                                   tatCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGGTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTa                                                                                   >  1:1811909/1‑140 (MQ=255)

                                                    atCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGACCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTag                                                                                  <  2:1003478/140‑1 (MQ=255)

                                                      cGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTagag                                                                                <  2:3497955/140‑1 (MQ=255)

                                                        gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATTTGGCGTTACAACTCGA‑TGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAggg                                                                              >  1:144487/1‑140 (MQ=255)

                                                        gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGTTTGAGGTGGCGCTGGCTCATTGCGCTCAGCCCTGAGGGGAGAggg                                                                              >  2:9232/1‑140 (MQ=255)

                                                        gAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAggg                                                                              >  1:4602844/1‑140 (MQ=255)

                                                           cTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGaa                                                                           >  2:3397637/1‑140 (MQ=255)

                                                           cTGACCGGACTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGCTACAACT‑GGATGAAGAGCGGTAGGTTGTGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCGCCTCAGCGCGAAGGGAAGAGGGGaa                                                                           >  2:4614443/1‑140 (MQ=255)

                                                              aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  1:3131171/1‑140 (MQ=255)

                                                              aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  2:4756182/1‑140 (MQ=255)

                                                              aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  1:4515947/1‑140 (MQ=255)

                                                              aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  1:3604858/1‑140 (MQ=255)

                                                              aCCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGc                                                                        >  2:4676083/1‑140 (MQ=255)

                                                              aCCGGGCTTGATCTGGGCAATTTTGATGACAGGTTGCTGCTGGATGTGGCGTTACAACT‑GGATGAAGAGCGGCCGGTTACGCGGTAAGGCTGAGGCGGCGCTGGCTCATTCCCCCCAGGCCGAAGGGTAGAGGGGAATGc                                                                        >  2:3765675/1‑140 (MQ=255)

                                                                cGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGccc                                                                      >  1:4955395/1‑140 (MQ=255)

                                                                   gCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGaa                                                                   >  1:186369/1‑140 (MQ=255)

                                                                   gCTCGATTGGGGCAATTTCGATGACAGGTTGCTGCTGTATGTGGCGTTCCAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAGGGGTAGAGGGGCATGCCCGaa                                                                   <  2:420369/140‑1 (MQ=255)

                                                                         tttGGGCAATTTTGATGACAGGTTGCTGCTGTATGGGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAAcc                                                             <  1:2120680/140‑1 (MQ=255)

                                                                           tGGGCACTTTTGATGACAGGTTGCCGCTGTATGTGCCGTTACCACT‑GGATAAAGAGCGGTAGGTTATGCGTCAAGGTTGAGGCGGCGCTGGCTCATCCCCCTCAGCCCGAAGGGTAGAGCGGAATGCCCGAATTAACCGt                                                           <  2:4896126/140‑1 (MQ=255)

                                                                           tGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                                           <  1:4268851/140‑1 (MQ=255)

                                                                           tGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                                           <  1:1721234/140‑1 (MQ=255)

                                                                           tGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGt                                                           <  1:1016102/140‑1 (MQ=255)

                                                                                aattgggatgaCAGGTTGCTGCTGTATGTGGCGTTACAACTGGG‑TGAAGAGCGGGAGCTTATGCGTTAAGGTTGAGGCGGCGCTTGCTCATTGCCCGCATCCCGAAGGGTAGAGGGGAATGCCCGAATTACCCGTCAGtt                                                      <  2:4460935/134‑1 (MQ=255)

                                                                                aaTTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGtt                                                      <  1:3512613/140‑1 (MQ=255)

                                                                                     tgatgaCAGGTTGCTGCTTTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGa                                                 <  2:3644070/140‑1 (MQ=255)

                                                                                          aCAGGTTGCTGCTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGattatt                                            >  2:2768265/1‑140 (MQ=255)

                                                                                                tgctgcTGTATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTAcgcg                                      <  1:3680468/140‑1 (MQ=255)

                                                                                                        tATGTGGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGtttt                              >  2:4161071/1‑140 (MQ=255)

                                                                                                          tgtgGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTc                            <  2:1350991/140‑1 (MQ=255)

                                                                                                          tgtgGCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTc                            >  2:4423968/1‑140 (MQ=255)

                                                                                                             ggCGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAg                         >  1:1076488/1‑140 (MQ=255)

                                                                                                               cGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTATCCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt                       >  2:4088329/1‑140 (MQ=255)

                                                                                                               cGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt                       >  2:2156494/1‑140 (MQ=255)

                                                                                                               cGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt                       >  1:3185893/1‑140 (MQ=255)

                                                                                                               cGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt                       >  1:2490232/1‑140 (MQ=255)

                                                                                                               cGTTACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGAt                       >  1:3759694/1‑140 (MQ=255)

                                                                                                                  tACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg                    >  1:225387/1‑140 (MQ=255)

                                                                                                                  tACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg                    >  1:335482/1‑140 (MQ=255)

                                                                                                                  tACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGAGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCgg                    >  1:420870/1‑140 (MQ=255)

                                                                                                                  tACAACT‑GGATGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATGCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATAgg                    >  2:3847851/1‑140 (MQ=255)

                                                                                                                         tggatGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCaa              <  1:4817064/136‑1 (MQ=255)

                                                                                                                         tggatGAAGAGCCGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATGGGCTTCaa              <  2:3601526/136‑1 (MQ=255)

                                                                                                                          ggatGAAGAGCGGTAGGTTATGCGTTAATGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGTTTCAAt             >  2:1855472/4‑140 (MQ=255)

                                                                                                                            atGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAAtct           <  2:3407988/139‑1 (MQ=255)

                                                                                                                             tGAAGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAAtctc          >  1:1543392/1‑140 (MQ=255)

                                                                                                                               aaGAGCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGc        <  1:4169977/140‑1 (MQ=255)

                                                                                                                                agagCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCt       >  1:4584119/1‑140 (MQ=255)

                                                                                                                                agagCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCt       <  1:1641531/140‑1 (MQ=255)

                                                                                                                                   gCGGTAGGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGAt    <  2:1620747/140‑1 (MQ=255)

CAAAGGCGTTGTTTATTCATCGTAATACCCTGGAGTATCGGCTTAATCGTATATCGGAACTGACCGGGCTTGATTTGGGCAATTTTGATGACAGGTTGCTGCTGTATGTGGCGTTACAACT‑GG‑TGAAGAGCGGTAAGTTATGCGTTAAGGTTGAGGCGGCGCTGGCTCATTCCCCTCAGCCCGAAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCT  >  NZ_CP025268/183315‑183585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`