BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,016,740	T→C	105 (TAA→TAG)	fliE ←	flagellar hook‑basal body complex protein FliE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,016,740	0	T	C	99.2%	802.5 / NA	241	105 (TAA→TAG)	fliE	flagellar hook‑basal body complex protein FliE
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (167/72); minor base G (2/0); total (169/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

GTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTCACCGAGA  >  NZ_CP025268/2016603‑2016875

gTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTa                                                                                                                                       <  2:1977730/140‑1 (MQ=255)

gTTATCGCGTATAAGGATCGTGGATGGCAGAAACCGTTCACGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCACATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTa                                                                                                                                       <  2:3478754/140‑1 (MQ=255)

 ttATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTac                                                                                                                                      >  1:4704905/1‑140 (MQ=255)

 ttATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTac                                                                                                                                      >  1:2242780/1‑140 (MQ=255)

 ttATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATGTTAAACCAGAAATTGAGTGAGGTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTac                                                                                                                                      >  2:257136/1‑140 (MQ=255)

 ttATCGCATATACGGATCGTGGATGGCAGAAAACGTTCAGGGTCAGGAATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTGATTAACAACAGGTTACAAAACCTac                                                                                                                                      >  2:2819486/1‑140 (MQ=255)

 ctATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCCGGTATCCATGTTAAACCAGAAGTTAAGTGAGTCTGCCCCATCCATGCCCCACGCGGTGAGATCAGATGTAGTTAACAACAGGTTACAAAACCTac                                                                                                                                      >  2:3306196/2‑140 (MQ=255)

   aTCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGACATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTacac                                                                                                                                    >  1:3723545/1‑140 (MQ=255)

   aTCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGATACAAAACGTacac                                                                                                                                    >  2:2213205/1‑140 (MQ=255)

      gcgTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGGATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTg                                                                                                                                 >  1:3486184/1‑140 (MQ=255)

       cgTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCGGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGc                                                                                                                                <  1:2602571/140‑1 (MQ=255)

       cgTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGc                                                                                                                                <  1:1009137/140‑1 (MQ=255)

       cgTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGc                                                                                                                                <  2:2701004/140‑1 (MQ=255)

         aataCGGATCGTGGATGGCAGAAAACGTTCAGGATCGGGGATCCATTTTAAACCAGAAATCGAGTGAGTTTGCCCCATCCAGGCCCCAGGCGGTGAGCTGGGATGTAACTAGCAACAGGCTACCAAAGCTACACCTGCAt                                                                                                                              >  2:4232780/2‑140 (MQ=255)

               gATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAATACCTACACCTGCATGCtcat                                                                                                                        <  1:5096489/140‑1 (MQ=255)

                aTCGTGTATGGCAGAACACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAACACCTACACCTGCATGCtcatc                                                                                                                       >  1:1093797/1‑140 (MQ=255)

                aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGc                                                                                                                                >  2:1151583/1‑131 (MQ=255)

                aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCtcatc                                                                                                                       >  1:3973328/1‑140 (MQ=255)

                aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCtcatc                                                                                                                       >  1:1624969/1‑140 (MQ=255)

                aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCtcatc                                                                                                                       >  2:635238/1‑140 (MQ=255)

                aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCtcatc                                                                                                                       >  1:29387/1‑140 (MQ=255)

                aTCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCtcatc                                                                                                                       >  1:603256/1‑140 (MQ=255)

                  cGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCAc                                                                                                                     >  1:4743389/1‑140 (MQ=255)

                  cGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCAc                                                                                                                     >  2:4481859/1‑140 (MQ=255)

                  cGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCAc                                                                                                                     >  2:1733099/1‑140 (MQ=255)

                  cGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCAc                                                                                                                     >  1:1107613/1‑140 (MQ=255)

                  cGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCAc                                                                                                                     >  1:3401293/1‑140 (MQ=255)

                   gTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACt                                                                                                                    >  1:1553288/1‑140 (MQ=255)

                    tGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACtt                                                                                                                   >  1:92717/1‑140 (MQ=255)

                          gCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATg                                                                                                                             <  1:4279325/124‑1 (MQ=255)

                          gCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATg                                                                                                                             >  2:4279325/1‑124 (MQ=255)

                            aGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAACCCTACACCTGCATGCTCATCACTTCCTGATAc                                                                                                           >  2:1597249/1‑140 (MQ=255)

                              aaaaCGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATAcgc                                                                                                         <  2:702925/140‑1 (MQ=255)

                              aaaaCGTACAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATAcgc                                                                                                         >  2:3418084/1‑140 (MQ=255)

                                   gTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGcca                                                                                                    <  1:1211686/140‑1 (MQ=255)

                                    ttCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGccac                                                                                                   <  2:767320/140‑1 (MQ=255)

                                    ttCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGccac                                                                                                   <  2:2620578/140‑1 (MQ=255)

                                    ttCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGccac                                                                                                   <  1:702082/140‑1 (MQ=255)

                                     tCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGccacc                                                                                                  >  2:804224/1‑140 (MQ=255)

                                      cAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGccacca                                                                                                 <  1:3868803/140‑1 (MQ=255)

                                        ggATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGc                                                                                               >  1:3896763/1‑140 (MQ=255)

                                             aGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCttatt                                                                                          >  1:3530877/1‑140 (MQ=255)

                                              ggTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCttatta                                                                                         >  1:4544509/1‑140 (MQ=255)

                                              ggTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCttatta                                                                                         >  1:2459806/1‑140 (MQ=255)

                                              ggTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCttatta                                                                                         <  2:4772381/140‑1 (MQ=255)

                                                tATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACg                                                                                       <  1:4449962/140‑1 (MQ=255)

                                                   ccATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCAc                                                                                    <  1:465170/140‑1 (MQ=255)

                                                   ccATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCAc                                                                                    >  1:256856/1‑140 (MQ=255)

                                                       tttAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGa                                                                                >  2:1486425/1‑140 (MQ=255)

                                                          aaaCCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATc                                                                             <  2:4580980/140‑1 (MQ=255)

                                                            aCCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATccc                                                                           >  2:4921077/1‑140 (MQ=255)

                                                                gAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCtcat                                                                                                                        <  2:4988267/91‑1 (MQ=255)

                                                                 aaaTTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCAttt                                                                      >  1:1979328/1‑140 (MQ=255)

                                                                       agtgagtTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAg                                                                >  1:4716449/1‑140 (MQ=255)

                                                                           agtTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAAc                                                            >  1:1521230/1‑140 (MQ=255)

                                                                           agtTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAAc                                                            >  1:2747086/1‑140 (MQ=255)

                                                                            gtTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACt                                                           <  1:2247000/140‑1 (MQ=255)

                                                                                     atccatGCCCCACGCGGTGAGATCGGATGTAATTAACAACCGGCTACAAAACCTACACCTGCATGCTGATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCCTAGAAAGCAAGGCtttt                                                  >  2:3731399/1‑140 (MQ=255)

                                                                                      tccatGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCGTAGAAACTGAGGCttttt                                                 >  2:4539181/1‑140 (MQ=255)

                                                                                           gCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCata                                            >  2:4045848/1‑140 (MQ=255)

                                                                                           gCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCata                                            >  1:1415422/1‑140 (MQ=255)

                                                                                           gCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTCGCATAGAAACTGAGGCTTTTTGCata                                            >  1:87100/1‑140 (MQ=255)

                                                                                                aCGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGt                                       >  1:2476749/1‑140 (MQ=255)

                                                                                                aCGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCCCATCACTTCCTGATACTCCGCCATCAGCTTATTACGCACCTGAATCCCCATTTCTATAGAAACGGACCCGTTTTGCCCATCgcc                                       >  2:3901698/1‑138 (MQ=255)

                                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCggac                                      >  2:3128347/1‑138 (MQ=255)

                                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtc                                      >  2:3372558/1‑140 (MQ=255)

                                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtc                                      >  2:843572/1‑140 (MQ=255)

                                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtc                                      >  1:2132066/1‑140 (MQ=255)

                                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtc                                      >  2:3244402/1‑140 (MQ=255)

                                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtc                                      >  2:903554/1‑140 (MQ=255)

                                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtc                                      >  1:3062335/1‑140 (MQ=255)

                                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtc                                      >  1:2401375/1‑140 (MQ=255)

                                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtc                                      >  2:2478218/1‑140 (MQ=255)

                                                                                                 cgcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACCGAGGCTTTTTGCATATCGGtc                                      >  2:1233407/1‑140 (MQ=255)

                                                                                                  gcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGGATGCTCATCAATTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtca                                     <  2:1013346/140‑1 (MQ=255)

                                                                                                  gcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtca                                     >  1:2083210/1‑140 (MQ=255)

                                                                                                  gcgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtca                                     >  1:1952475/1‑140 (MQ=255)

                                                                                                   cgGTGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGtcat                                    <  2:3046507/140‑1 (MQ=255)

                                                                                                      tGAGATCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCGTCGCTTCCTGATACGGCGCCACCAGCTTAGTACGCACCTGAATCCCCATTTGCATAGAAACGGAGGATTTTTGCATATCGGTCATcac                                 >  2:2733348/1‑140 (MQ=255)

                                                                                                      tGAGATCGGATGTAATTAACAACAAGTTACAAAACCTAAACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTTCATAGAAACTGAGGCTTTTTGCATATCGGTCATcac                                 >  2:5103005/1‑140 (MQ=255)

                                                                                                       gagaTCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATcaca                                <  1:4965089/140‑1 (MQ=255)

                                                                                                       gagaTCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATcaca                                <  2:1323619/140‑1 (MQ=255)

                                                                                                       gagaTCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATcaca                                <  1:1695201/140‑1 (MQ=255)

                                                                                                       gagaTCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATcaca                                <  1:4101591/140‑1 (MQ=255)

                                                                                                        agaTCGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACAt                               <  1:1319534/140‑1 (MQ=255)

                                                                                                            cGGATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATAcg                                                                                                          >  1:4826635/1‑61 (MQ=255)

                                                                                                              gATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTa                         >  2:3148940/1‑140 (MQ=255)

                                                                                                              gATGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTa                         >  1:3486652/1‑140 (MQ=255)

                                                                                                                tGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCtcat                                                                                                                        <  2:4810936/43‑1 (MQ=255)

                                                                                                                tGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCtcat                                                                                                                        >  1:4810936/1‑43 (MQ=255)

                                                                                                                tGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAac                       >  1:3032861/1‑140 (MQ=255)

                                                                                                                tGTAATTAACAACAGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAac                       >  2:2042051/1‑140 (MQ=255)

                                                                                                                           acaGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCggg            <  2:3660857/140‑1 (MQ=255)

                                                                                                                            caGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGTTCATCACATCGTTTAACGCCACGCCGGGt           >  2:3445689/1‑140 (MQ=255)

                                                                                                                            caGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATACCGGTCATCACATCGTTTAACGCCACGCCGGGt           >  2:4448633/1‑140 (MQ=255)

                                                                                                                            caGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTGGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTATAACGCCACGCCGGGt           >  1:2838222/1‑140 (MQ=255)

                                                                                                                             aGGTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGtt          >  1:4672629/1‑140 (MQ=255)

                                                                                                                               gTTACAAAACCTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCGTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTTCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTCa        >  1:1810785/1‑140 (MQ=255)

                                                                                                                                 tACAAAACCTACACCTGCATGCTCATCACTTCCTTATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTCAcc      >  1:406619/1‑140 (MQ=255)

                                                                                                                                   cAAAACCTACACCTGCATGCTCATCACTCCCTGATACGCCGCCACCAGCTTCTTACGCACCTGAATCCCCATTTGCATCGAAACTGAGGCTCTTTGCATATCGGTCATCACATCTGTTAACGCCGCCCCGGGCTCAcctg    >  2:289778/1‑138 (MQ=255)

GTTATCGCGTATACGGATCGTGGATGGCAGAAAACGTTCAGGATCAGGTATCCATTTTAAACCAGAAATTGAGTGAGTTTGCCCCATCCATGCCCCACGCGGTGAGATCGGATGTAATTAACAACAGGTTACAAAACTTACACCTGCATGCTCATCACTTCCTGATACGCCGCCACCAGCTTATTACGCACCTGAATCCCCATTTGCATAGAAACTGAGGCTTTTTGCATATCGGTCATCACATCGTTTAACGCCACGCCGGGTTCACCGAGA  >  NZ_CP025268/2016603‑2016875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`