BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	184,087	T→C	271 (TAA→TAG)	yaeI ←	phosphodiesterase YaeI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	184,087	0	T	C	99.5%	732.7 / NA	220	271 (TAA→TAG)	yaeI	phosphodiesterase YaeI
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (87/131); total (89/131)
Fisher's exact test for biased strand distribution p-value = 4.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

ATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACA  >  NZ_CP025268/183952‑184222

 ttGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTacac                                                                                                                                    >  1:536674/1‑140 (MQ=255)

 ttGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTacac                                                                                                                                    <  1:2746115/140‑1 (MQ=255)

 ttGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTacac                                                                                                                                    <  1:4135688/140‑1 (MQ=255)

  tgtgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACAcc                                                                                                                                   <  1:329582/140‑1 (MQ=255)

    tgCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAg                                                                                                                                 >  1:3380144/1‑140 (MQ=255)

     gCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGt                                                                                                                                >  1:1955662/1‑140 (MQ=255)

       ttATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTTATCACAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATGAAACAATTACCTGCTTTATTCTGGTGATAAAATGCACGAGCTACACAATTTc                                                                                                                              >  2:1820405/1‑140 (MQ=255)

           tttAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGc                                                                                                                          <  1:4848049/140‑1 (MQ=255)

           tttAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGc                                                                                                                          <  1:2231211/140‑1 (MQ=255)

           tttAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGc                                                                                                                          <  2:2898431/140‑1 (MQ=255)

                    tggcctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACt                                                                                                                 >  1:3600334/1‑140 (MQ=255)

                    tggcctcgccCGCCCGGCACGACTGTGATCGCCGACAGCCACTTTCTTCCAGACTTCTATCAGCGCTTAAAAAACAAAAAATTTCCCGCTTTACTCTGGCGATTAAATTCACCAGACACCCCATTTCCAGCACCGTCACt                                                                                                                 >  2:5071712/1‑140 (MQ=255)

                       cctggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTcc                                                                                                              <  1:794513/140‑1 (MQ=255)

                          ggccCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCggg                                                                                                           >  1:2287987/1‑140 (MQ=255)

                           gcccgcccGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggc                                                                                                          >  1:1199522/1‑140 (MQ=255)

                             ccgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                        <  1:1436152/140‑1 (MQ=255)

                             ccgcccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                        <  1:4971731/140‑1 (MQ=255)

                             ccgcacgGCTCGACTGTGATCGCAGAAAGCATTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTTTTTTAATGATACGGCGACCACCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGggcgg                                                                                                        <  2:229187/140‑1 (MQ=255)

                              cgcccgGCTCGACTGTGATCGCAGAAAGACTTTTTCTTCCAGCCTTCTATCCTGGCGTAAAAATCACAAACTTACCTGCTTTATTCTGGTGATACAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGggcggc                                                                                                       <  1:1125128/140‑1 (MQ=255)

                                cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                     >  1:2011170/1‑140 (MQ=255)

                                cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                     >  1:3789364/1‑140 (MQ=255)

                                cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                     >  1:2513934/1‑140 (MQ=255)

                                cccgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAg                                                                                                     >  1:3230523/1‑140 (MQ=255)

                                  cgGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTACCATGGCGTAAAAATCAAAAAATTACCTTTTTTAATGATACGGCGACCACCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGtt                                                                                                   <  2:2884589/140‑1 (MQ=255)

                                   ggCTCGACTGTGATCTCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTTTTTTAATGATACGGCGACCACCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTc                                                                                                  <  2:4955395/140‑1 (MQ=255)

                                     cTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCCTGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCCGTTCGAGCATCGTTACTTCCGGGCGGCAGTTCAg                                                                                                >  1:2417659/1‑140 (MQ=255)

                                       cGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGGCGACCTCCGCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                              <  2:1854422/140‑1 (MQ=255)

                                       cGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGAc                                                                                              <  1:1100797/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTTTTTTAATGATACGACGACCACCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  2:4105447/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGTTTTATTGAGACGGCAAACACCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  2:4584119/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  1:681559/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  2:3230523/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  1:2497274/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  1:1169743/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  1:3042334/140‑1 (MQ=255)

                                           tgtgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCAGGCGACAAACTCCGCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  2:1898793/140‑1 (MQ=255)

                                           tctgATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTTCTTTAATGATACGGCGACCACCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCaa                                                                                          <  2:4186217/138‑1 (MQ=255)

                                                tCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCCGACGCAAACCAt                                                                                     <  1:5020875/140‑1 (MQ=255)

                                                    aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTGTTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACaa                                                                                 >  2:2912604/1‑140 (MQ=255)

                                                    aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACaa                                                                                 >  1:2668721/1‑140 (MQ=255)

                                                    aGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACaa                                                                                 >  2:1300921/1‑140 (MQ=255)

                                                     gAAAGCCTCTGTCTTCCAGACTTCTATCATGGCGAAAAAATCAAAAAATTACCTGACTTATTCTGGCGATAAAATTCACGATCTACACCAGCTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACaaa                                                                                <  2:3725605/140‑1 (MQ=255)

                                                       aaGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACt                                                                              >  1:349438/1‑140 (MQ=255)

                                                          ccTTTTTCGTGCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATACTGGTGATAAACTTCGCGATCTACACCAGTTCCACCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGcc                                                                           <  2:3734186/140‑1 (MQ=255)

                                                           cTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGccc                                                                          <  2:310130/140‑1 (MQ=255)

                                                                tcttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTTCTTTAATGATACGACAACCACCGAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcc                                                                     <  2:3556430/140‑1 (MQ=255)

                                                                tcttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTTCTTTAATGAGGCGACAACCTCCGCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcc                                                                     <  2:3809483/140‑1 (MQ=255)

                                                                tcttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcc                                                                     <  1:751880/140‑1 (MQ=255)

                                                                tcttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCAGACAAACTGCccacgcc                                                                     <  2:1243810/140‑1 (MQ=255)

                                                                 cttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTGTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcca                                                                    <  2:2740346/140‑1 (MQ=255)

                                                                 cttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgcca                                                                    <  2:2620490/140‑1 (MQ=255)

                                                                  ttcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccac                                                                   <  1:4624730/140‑1 (MQ=255)

                                                                   tcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTTGTGATAACATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccacg                                                                  <  2:4262428/140‑1 (MQ=255)

                                                                   tcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAACATCCGCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccacg                                                                  <  2:3757883/140‑1 (MQ=255)

                                                                   tcCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccacgccacg                                                                  <  2:3457282/140‑1 (MQ=255)

                                                                    ccAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACgg                                                                 >  1:4775966/1‑140 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCACAAAATTACCTTCTTTAATCTGGCGACAAACTCCGCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  2:608161/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAACCTGCCCACGCCACggg                                                                <  1:1389698/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:3209968/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:4509255/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:4576194/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:29591/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:5025777/140‑1 (MQ=255)

                                                                     cAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACggg                                                                <  1:4712299/140‑1 (MQ=255)

                                                                            cTATCCGGGCGTAAAAATCAAAAAGTTACCTGCTTTACTCTGGGGATAAAATTCACGAACGACACCAGTTCCAGAATCGTTACTTCCGGGCGGCAGTTCAAACGCAAACCATACAAACTGCCCACGCCACGTGTTGCGTa                                                         >  2:2419830/1‑140 (MQ=255)

                                                                            cTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTa                                                         >  1:979941/1‑140 (MQ=255)

                                                                                cATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAtg                                                     <  1:1661650/140‑1 (MQ=255)

                                                                                  tGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAtgtg                                                   >  2:2255076/1‑140 (MQ=255)

                                                                                    gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGAtctcgg                                                 >  2:1399511/1‑135 (MQ=255)

                                                                                    gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTc                                                 >  2:648361/1‑140 (MQ=255)

                                                                                    gCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTc                                                 >  2:1143697/1‑140 (MQ=255)

                                                                                      gTAAAAATCAAAAAATTACCTGTTTTATTCTGGTGATAAACTTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCtt                                               <  2:4113369/140‑1 (MQ=255)

                                                                                      gTAAAAATCAAAAAATTAACTTCTTTAATCTTGCGGTGACCATCAAGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCtt                                               <  2:281025/140‑1 (MQ=255)

                                                                                       tAAAAATCAAAAAATTACCTGCTTAAATCTGGACATAAAATTCACGATCTAAACCAGTTCCAGCATTGTTACTTCCGGGTGTCAGTTCAGACGCAAACAATACAAACTGCCCACGCCACGGGATGTGTAGATGTGTCtct                                              >  2:1185018/1‑138 (MQ=255)

                                                                                              cAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCGCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCaaa                                       <  2:1595068/140‑1 (MQ=255)

                                                                                                        ccTGCTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAAt                             >  1:3895480/1‑140 (MQ=255)

                                                                                                            cTTTATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCgg                         <  2:1955662/140‑1 (MQ=255)

                                                                                                             tttATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGc                        >  1:4957591/1‑140 (MQ=255)

                                                                                                               tATTCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGa                      >  1:113262/1‑140 (MQ=255)

                                                                                                                 ttCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACg                    <  1:1854509/140‑1 (MQ=255)

                                                                                                                 ttCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACg                    <  1:4088329/140‑1 (MQ=255)

                                                                                                                 ttCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACg                    <  1:3385086/140‑1 (MQ=255)

                                                                                                                  tCTGGTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGt                   >  1:3008842/1‑140 (MQ=255)

                                                                                                                      gTGATAAAATTCACGGTCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGGAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGTTGTGTCTTTCGCCAAAGGCATTGAATCCGGCGACGTAACg               >  2:4033616/1‑140 (MQ=255)

                                                                                                                      gTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACg               >  2:557866/1‑140 (MQ=255)

                                                                                                                      gTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACg               >  2:3251457/1‑140 (MQ=255)

                                                                                                                      gTGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTGTCGGCAGAGGCATTAAATCAGGCGACGTaagg               >  2:22445/1‑138 (MQ=255)

                                                                                                                      gTGATAAAATTCACGATCTACACCAGTTCCAGCATCGGTACTTCAGGACAGCAATTCGGACGCAAACCATACAAACTGCCCAAGCCACGGGTTGTGTAGGTGTGTCTTTAGCAGAGGGCATTTAATACGAGGACGGaagg               >  2:4633173/1‑138 (MQ=255)

                                                                                                                       tGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt              <  1:4370272/140‑1 (MQ=255)

                                                                                                                       tGATAAAATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt              <  2:4747610/140‑1 (MQ=255)

                                                                                                                       cgATAACATCCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGt              <  2:628324/139‑1 (MQ=255)

                                                                                                                        gATAATATTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGtt             <  2:4478773/140‑1 (MQ=255)

                                                                                                                         aTAAAATTCACGAGCTACACAAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAAGGttt            >  2:3847033/1‑140 (MQ=255)

                                                                                                                           aaaaTTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCccac                                                                        >  1:3751504/1‑78 (MQ=255)

                                                                                                                           aaaaTTCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTAt          >  1:4694899/1‑140 (MQ=255)

                                                                                                                                tCACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCg     >  1:2928547/1‑140 (MQ=255)

                                                                                                                                tCACGATCGACACCAGTTCCAGCATCGTTACGTCCGGGCGGCAGTTCAGACGCAAACCATCCAAACTGCCCACGTAACGGGTTGTGTAGATTTGGGTGTCGCCACAGGCATTTAATCCGGCGACGACACGTTTAtactcg     >  2:1431433/1‑135 (MQ=255)

                                                                                                                                 cACGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGa    <  1:107927/140‑1 (MQ=255)

                                                                                                                                  aCGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGAc   >  1:1659874/1‑140 (MQ=255)

                                                                                                                                   cGATCTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACa  <  1:2186321/140‑1 (MQ=255)

ATTGTGCTTATTTTAGCATTTGGCCTGGCCCGCCCGGCTCGACTGTGATCGCAGAAAGCCTTTTTCTTCCAGACTTCTATCATGGCGTAAAAATCAAAAAATTACCTGCTTTATTCTGGTGATAAAATTCACGATTTACACCAGTTCCAGCATCGTTACTTCCGGGCGGCAGTTCAGACGCAAACCATACAAACTGCCCACGCCACGGGTTGTGTAGATGTGTCTTTCGCCAAAGGCATTTAATCCGGCGACGTAACGTTTATCTTCGACA  >  NZ_CP025268/183952‑184222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`