BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,089,870	G→A	P411L (CCG→CTG)	plaP ←	putrescine/proton symporter PlaP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,089,870	0	G	A	100.0%	785.4 / NA	211	P411L (CCG→CTG)	plaP	putrescine/proton symporter PlaP
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (166/45); total (166/45)

Only 100 of 212 total aligned reads displayed.

GAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGC  >  NZ_CP025268/2089733‑2090006

gAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAgc                                                                                                                                        >  2:2978729/1‑140 (MQ=255)

      atgatTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCa                                                                                                                                  <  1:1052635/140‑1 (MQ=255)

      atgatTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCa                                                                                                                                  <  1:3466290/140‑1 (MQ=255)

         atTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACg                                                                                                                               <  1:1513560/140‑1 (MQ=255)

             tGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGACAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCa                                                                                                                           <  2:4636329/140‑1 (MQ=255)

                 aCGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAgcgc                                                                                                                       <  1:843053/140‑1 (MQ=255)

                  cGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGca                                                                                                                      >  2:799888/1‑140 (MQ=255)

                  cGTCTTCGTACTGCGGAACCGGAGTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGca                                                                                                                      >  1:3061685/1‑140 (MQ=255)

                   gTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGcac                                                                                                                     <  1:4942031/140‑1 (MQ=255)

                    tCTTCGTACTGCGGAACGGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTAAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGaacc                                                                                                                    >  2:6516/1‑140 (MQ=255)

                    tCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGcacc                                                                                                                    >  2:3282621/1‑140 (MQ=255)

                    tCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGcacc                                                                                                                    >  1:2219325/1‑140 (MQ=255)

                       tCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACcaca                                                                                                                 >  2:3157383/1‑140 (MQ=255)

                       tCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACcaca                                                                                                                 >  2:3157700/1‑140 (MQ=255)

                       tCGTACTGCGGAACCGGATTACGGAAGCTCCTGGTGACGCAAGCCAGGTAAACCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACcaca                                                                                                                 >  2:3596729/1‑140 (MQ=255)

                        cGTACTGCGGAACCGGCTTACGGAAGCTCTTGGTGACCCAAGCCCCGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCAGTGAGCTTTCTTCCAGGTTAACCGACAGCGCACCAACGGTCAGCGCACcacac                                                                                                                >  2:4457407/1‑140 (MQ=255)

                        cGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACcacac                                                                                                                >  2:2659250/1‑140 (MQ=255)

                         gTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACcacaca                                                                                                               >  1:4304149/1‑140 (MQ=255)

                              gCGGAACCGGATTACGGAAGCTGTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                          <  2:4788924/140‑1 (MQ=255)

                              gCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                          >  1:2558626/1‑140 (MQ=255)

                              gCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                          >  1:1916749/1‑140 (MQ=255)

                              gCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGc                                                                                                          >  2:2574198/1‑140 (MQ=255)

                               cGGAACGGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGGAAATCTGACCGGTAACAGCCCCGATCAGACCCAGCACCATTGAGCTTTCTTGCAGGGTAACCCACAGCGCACCAACGGTCAGCGCCCCACACATCGGCa                                                                                                         >  2:3363051/1‑140 (MQ=255)

                               cGGAACCGGATTACGGAAGCTCTTTGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCGTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                         >  1:1703085/1‑140 (MQ=255)

                               cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                         >  2:2592622/1‑140 (MQ=255)

                               cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                         >  1:3712683/1‑140 (MQ=255)

                               cGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCa                                                                                                         >  1:661031/1‑140 (MQ=255)

                               cGGAACCGGATTACGGAAGCTCTTAGTGACGCAAGCCAGGTACATCAGACCGATAGCAGCCCAGATCAGACCAAGAACCATTGAGCTTTCATCCAGGTTAACCAACAGCGCACCAACGGTCAGCGCAACACACATCGGCa                                                                                                         >  2:3833667/1‑140 (MQ=255)

                                 gAACCGGATGACGGAGGCGTGTGGTGACGCAAGCCAGGGAAAACAGACCGAGAGCAGCCCATATCAGACCCAGAACCATTGAGCGTTCTGCCAATTTAACCCACAGCGCCCTAACGGTCAGCGCAACCCACCTCGGCAgg                                                                                                       >  2:2182117/1‑140 (MQ=255)

                                  aaCCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGa                                                                                                      >  1:2497938/1‑140 (MQ=255)

                                   aCCGCATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGaa                                                                                                     <  1:3898710/140‑1 (MQ=255)

                                     cGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATATCAGCCCAGATCAGACCCAGAACCATTGTGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACa                                                                                                   <  2:2466177/140‑1 (MQ=255)

                                       gATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGACCAGa                                                                                                 >  1:4192520/1‑140 (MQ=255)

                                       gATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGa                                                                                                 >  2:3876236/1‑140 (MQ=255)

                                       gATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGa                                                                                                 >  1:565081/1‑140 (MQ=255)

                                                 gCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTGCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGt                                                                                       <  1:799889/140‑1 (MQ=255)

                                                  ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAggg                                                                                      >  1:1193681/1‑138 (MQ=255)

                                                  ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTg                                                                                      >  1:4549249/1‑140 (MQ=255)

                                                  ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTg                                                                                      >  2:2331528/1‑140 (MQ=255)

                                                  ctctTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTg                                                                                      <  1:1253235/140‑1 (MQ=255)

                                                      tGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCt                                                                                  >  1:4496364/1‑140 (MQ=255)

                                                          gACGCAAGACAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCa                                                                              <  2:1514578/140‑1 (MQ=255)

                                                           aCGCAAGCCAGGTACATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAg                                                                             >  1:4772797/1‑140 (MQ=255)

                                                           aCGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACGGGAAGTGATCTTTCAg                                                                             >  1:797590/1‑140 (MQ=255)

                                                             gCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                           >  2:897856/1‑140 (MQ=255)

                                                             gCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCg                                                                           >  2:4302720/1‑140 (MQ=255)

                                                                 gCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCtt                                                                       >  1:217310/1‑140 (MQ=255)

                                                                 gCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCtt                                                                       >  1:1954875/1‑140 (MQ=255)

                                                                     ggTAAATCAGACCGATAGCAGACCAGATCAGACCCAGAACCATTGAGCTTTCTTACAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTa                                                                   <  1:1387400/140‑1 (MQ=255)

                                                                           tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCGACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGAGACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTtc                                                             >  2:3063958/1‑140 (MQ=255)

                                                                           tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTtc                                                             >  1:2986971/1‑140 (MQ=255)

                                                                           tCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTtc                                                             >  1:2285509/1‑140 (MQ=255)

                                                                               aCCGATAGCAGCCCAGATCAGACCCGGAACCATTGCGCTTTCGTCCAGGGTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTAATCGTTCAGCGTCTTGTTACGCTCCTCAc                                                         >  2:4123188/1‑140 (MQ=255)

                                                                               aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                         <  1:1687370/140‑1 (MQ=255)

                                                                               aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                         <  1:1781500/140‑1 (MQ=255)

                                                                               aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                         <  1:1263793/140‑1 (MQ=255)

                                                                               aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                         >  2:205098/1‑140 (MQ=255)

                                                                               aCCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCAc                                                         >  1:109641/1‑140 (MQ=255)

                                                                                ccGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACg                                                        >  1:3563210/1‑140 (MQ=255)

                                                                                 cGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACgg                                                       <  2:1814234/140‑1 (MQ=255)

                                                                                   aTAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGAt                                                     >  1:5014827/1‑140 (MQ=255)

                                                                                    tAGCAGCCCCGATCAGACCCAGAATCATGGACCTGTCTTGCAGGTTGACCAACAGGGGACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTGCAGCGTCTTGTTACGCTTCTCACGGCTc                                                    >  2:2877162/1‑140 (MQ=255)

                                                                                    tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATc                                                    >  2:3244378/1‑140 (MQ=255)

                                                                                    tAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGGCTTGTTACGCTTCCCACGGATc                                                    >  2:818234/1‑140 (MQ=255)

                                                                                      gcagcCCAGATCAGACCAAGAACCATCGAGCTTTGCCCCAGGTTAACCCACAGCGAACCAACGGTCAGCGCACCACACATCGGCAGGAACAAATACCGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCa                                                  <  2:469578/140‑1 (MQ=255)

                                                                                         gcCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGaa                                               >  2:919408/1‑140 (MQ=255)

                                                                                              gATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCg                                          >  1:2511213/1‑140 (MQ=255)

                                                                                                    aCCCAGAACCCTTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGa                                    >  1:3365983/1‑140 (MQ=255)

                                                                                                    aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGa                                    >  1:4348789/1‑140 (MQ=255)

                                                                                                    aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGa                                    >  1:1027409/1‑140 (MQ=255)

                                                                                                    aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGa                                    >  2:1003120/1‑140 (MQ=255)

                                                                                                    aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGa                                    >  2:3251946/1‑140 (MQ=255)

                                                                                                    aCCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGa                                    >  2:333457/1‑140 (MQ=255)

                                                                                                    aCCCAGAACCATTGAGCTTTCTTCCAGGGTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGa                                    >  2:463981/1‑140 (MQ=255)

                                                                                                       cAGAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCg                                 <  1:3130430/140‑1 (MQ=255)

                                                                                                        aGAACAATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACGGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGa                                <  2:3910070/140‑1 (MQ=255)

                                                                                                         gAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGTATCCAGAACTGCGAGATGACCGAc                               >  1:2800082/1‑140 (MQ=255)

                                                                                                         gAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                               >  2:4954388/1‑140 (MQ=255)

                                                                                                         gAACCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGAc                               >  1:2378156/1‑140 (MQ=255)

                                                                                                           aCCATTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAg                             <  1:4095228/140‑1 (MQ=255)

                                                                                                              aTTGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTCCTCACGGATCCAGAACTGCGAGATGACCGACAggct                          >  2:3782360/1‑138 (MQ=255)

                                                                                                               ttGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCCCACATCGGCAGGAACAGATACTGGAAGTGACCTTCCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTa                         >  2:1552948/1‑140 (MQ=255)

                                                                                                               ttGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTa                         >  2:514039/1‑140 (MQ=255)

                                                                                                               tagAGCTTTCTTGCAGGTTAACCTACAGCGCACCAACGGTGAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTGTCAGCGTCGTGGTACGCTTCTCAAGGATCCAGAACTGCGAGTTGACCGACAGGTTa                         >  2:1464140/3‑140 (MQ=255)

                                                                                                                tGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTaa                        >  1:4004992/1‑140 (MQ=255)

                                                                                                                tGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTaa                        >  2:666413/1‑140 (MQ=255)

                                                                                                                tGAGCTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTaa                        >  2:4703370/1‑140 (MQ=255)

                                                                                                                    cTTTCTTCCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGaa                    >  1:4743567/1‑140 (MQ=255)

                                                                                                                       tcttcCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGCTCTTTCAGCGTCTTGTTACGCTTCTCACGGCTCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGt                 >  1:3814872/1‑140 (MQ=255)

                                                                                                                       tcttcCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGt                 >  1:507119/1‑140 (MQ=255)

                                                                                                                         ttcCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATTCAGGACTGCGAGAGGACCGAGAGGGTAACGAAAGTGa               >  2:2227014/1‑140 (MQ=255)

                                                                                                                         ttcCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGa               >  1:5094101/1‑140 (MQ=255)

                                                                                                                         ttcCAGGTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCCTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGa               >  1:4710089/1‑140 (MQ=255)

                                                                                                                              ggTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAg                                                                                        >  2:169843/1‑62 (MQ=255)

                                                                                                                              ggTTAACCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATc                                                    >  2:449006/1‑98 (MQ=255)

                                                                                                                                   aCCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAg     >  2:3519708/1‑140 (MQ=255)

                                                                                                                                   aCCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAg     >  1:266527/1‑140 (MQ=255)

                                                                                                                                   aCCCACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAg     >  1:4668617/1‑140 (MQ=255)

                                                                                                                                    cccACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAgc    >  2:1339818/1‑140 (MQ=255)

                                                                                                                                    cccACAGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAgc    >  1:3628879/1‑140 (MQ=255)

GAAAAGATGATTATGCTACGTCTTCGTACTGCGGAACCGGATTACGGAAGCTCTTGGTGACGCAAGCCAGGTAAATCAGACCGATAGCAGCCCAGATCAGACCCAGAACCATTGAGCTTTCTTCCAGGTTAACCCACGGCGCACCAACGGTCAGCGCACCACACATCGGCAGGAACAGATACTGGAAGTGATCTTTCAGCGTCTTGTTACGCTTCTCACGGATCCAGAACTGCGAGATGACCGACAGGTTAACGAAAGTGAACGCCACCAGCGC  >  NZ_CP025268/2089733‑2090006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`