BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,154,002	T→G	G341G (GGA→GGC)	yegI ←	protein kinase YegI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,154,002	0	T	G	99.3%	942.8 / NA	285	G341G (GGA→GGC)	yegI	protein kinase YegI
Reads supporting (aligned to +/- strand): ref base T (0/2); new base G (101/181); total (102/183)
Fisher's exact test for biased strand distribution p-value = 5.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

CCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTA  >  NZ_CP025268/2153865‑2154138

   aaaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                     <  2:278236/140‑1 (MQ=255)

   aaaGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                     <  1:1295147/140‑1 (MQ=255)

   aaaGGAAGCGGCCTGCCAGTCGGGTGGAAATGATCGGGTAATGGCAGTTGCAATGTTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCAGCCGGTGGTAATGACCTCTTCGCCgag                                                                                                                                     <  2:4656890/140‑1 (MQ=255)

     aGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAt                                                                                                                                   <  1:1127268/140‑1 (MQ=255)

     aGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAt                                                                                                                                   <  1:1035291/140‑1 (MQ=255)

     aGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAt                                                                                                                                   <  1:2195159/140‑1 (MQ=255)

       gAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCa                                                                                                                                 <  1:2970164/140‑1 (MQ=255)

         aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAt                                                                                                                               >  1:1788384/1‑140 (MQ=255)

         aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAt                                                                                                                               >  2:2667821/1‑140 (MQ=255)

         aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAt                                                                                                                               >  1:1414499/1‑140 (MQ=255)

         aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAAt                                                                                                                               >  1:2728376/1‑140 (MQ=255)

         aGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCGGGCGGTGCTACTGACGCCGTCAGCATCGCCCAAACTTTAGGGAGCCACAACTTCCCGCCGGTGGTAATGAAATCTTCGCAGCGATCAAt                                                                                                                               >  2:860272/1‑140 (MQ=255)

         aGCGGCCTGCCAGTCGGTTGGAAAGGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCAAGCACAAAATTCCCGCCGGTGGTAAGGACCTCTACGCCGAGATCAAt                                                                                                                               >  2:4191617/1‑140 (MQ=255)

          gCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATa                                                                                                                              <  1:1155724/140‑1 (MQ=255)

            ggCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATaaa                                                                                                                            <  1:2528032/140‑1 (MQ=255)

            ggCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAGCAATaaa                                                                                                                            <  2:2624518/140‑1 (MQ=255)

             gCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATaaaa                                                                                                                           <  1:4158050/140‑1 (MQ=255)

                 gCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGa                                                                                                                       <  1:4789498/140‑1 (MQ=255)

                  ccAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGAt                                                                                                                      <  2:4742650/140‑1 (MQ=255)

                  ccAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGAt                                                                                                                      <  1:332942/140‑1 (MQ=255)

                    aGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATaa                                                                                                                    <  1:1494535/140‑1 (MQ=255)

                    aGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATaa                                                                                                                    <  1:4373276/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  1:2406912/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  1:1695521/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  2:4605145/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  1:374029/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGACGGTGGTAATGACATCTTCGACGAGATCAATAAAATAGATAacac                                                                                                                 <  2:286818/140‑1 (MQ=255)

                       cGGTTGGAAATGATCGGGTAATGGCAGTTACAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAacac                                                                                                                 <  1:1834971/140‑1 (MQ=255)

                         gTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCt                                                                                                               <  1:2094232/140‑1 (MQ=255)

                            ggcactGATCGTGTAATGGCAGTTGCAATGCTGGCGGTGCTACTTTCGCCATCACCATCGCCCAGTCTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATATATAACACCTTGa                                                                                                            <  2:1099072/135‑1 (MQ=255)

                            ggAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGa                                                                                                            >  2:4582070/1‑140 (MQ=255)

                            ggAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGa                                                                                                            <  1:1606281/140‑1 (MQ=255)

                            ggAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGa                                                                                                            <  2:3860392/140‑1 (MQ=255)

                                aTGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTAt                                                                                                        <  1:2603633/140‑1 (MQ=255)

                                aTGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTAt                                                                                                        <  2:2307340/140‑1 (MQ=255)

                                aTGATCGGGTAATCGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTAt                                                                                                        <  1:3991898/140‑1 (MQ=255)

                                 tGATCGGTTAATGGCAGTTGCAACGTCGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTACTGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATc                                                                                                       <  2:1040360/140‑1 (MQ=255)

                                    tCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAg                                                                                                    >  1:3178031/1‑140 (MQ=255)

                                      gggTAATGGCAGTTGCAAGGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAgcg                                                                                                  <  2:1579199/140‑1 (MQ=255)

                                        gTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTACCCAGCGca                                                                                                >  2:3718943/1‑140 (MQ=255)

                                         tAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGTCCGCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGcac                                                                                               <  2:1690842/140‑1 (MQ=255)

                                         tAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGcac                                                                                               <  2:3297840/140‑1 (MQ=255)

                                         tAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGcac                                                                                               <  1:1875857/140‑1 (MQ=255)

                                           ctGGCAGTTGCAATGCTGCCGGTGCTACTGACGCCATCACCATCGCCCAAACTTTCGCCAGCACAAAATTCCCGCCGTTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCacac                                                                                             <  2:3014407/139‑1 (MQ=255)

                                             ggCAGTTGCAATGCTGGCGTTGCTACTGACGCCATCACCATCGCCCAGCCTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGCTCAATAAAATAGATAACACCTTGATTATCCAGCGCAcacca                                                                                           >  2:4137770/1‑140 (MQ=255)

                                             ggCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCAcacca                                                                                           <  1:58255/140‑1 (MQ=255)

                                                  ttGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGc                                                                                      >  1:462809/1‑140 (MQ=255)

                                                  ttGCAATGCTGGCGGTGCTACTGACGACATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGAACAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGc                                                                                      >  1:2756166/1‑140 (MQ=255)

                                                  tcgccctGCTGGCGATGCTACCGACGACATCACCATCGCCCAGACTTTCGCGACCACAAACTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACCCCTTGATTATCCAGCGCACACCCCGGGc                                                                                      <  2:3378327/134‑1 (MQ=255)

                                                    gCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGcag                                                                                    <  2:3694064/140‑1 (MQ=255)

                                                                 tccTACTGACGGCATCACCATCGCCCCGACTTTCGCCAGCACAAAATTCCCGCCGGGGGTAATGACCTCTTCGCCGAGATCAATAAAAGAGATACCGCCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  2:3958913/138‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATCACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  2:2207151/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:707332/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:3362843/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:2505151/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:4667820/140‑1 (MQ=255)

                                                                 tGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAg                                                                       <  1:4707987/140‑1 (MQ=255)

                                                                  gCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGc                                                                      >  1:4104537/1‑140 (MQ=255)

                                                                      cTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCgggg                                                                  >  1:2865236/1‑140 (MQ=255)

                                                                              aTCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                          <  1:3257681/140‑1 (MQ=255)

                                                                              aTCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATg                                                          <  2:2017033/140‑1 (MQ=255)

                                                                                ccccACCGCCCAGACATTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCACATGAGCGGGGTAAACATGCa                                                        <  2:4309834/138‑1 (MQ=255)

                                                                                cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCa                                                        >  2:2232153/1‑140 (MQ=255)

                                                                                cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCa                                                        >  1:1542399/1‑140 (MQ=255)

                                                                                cACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATCAGCGGGGTAAACATGCa                                                        >  2:1811964/1‑140 (MQ=255)

                                                                                     tCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCt                                                   >  2:3560273/1‑140 (MQ=255)

                                                                                     tCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCt                                                   >  1:344697/1‑140 (MQ=255)

                                                                                       gCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGa                                                 <  2:3791058/140‑1 (MQ=255)

                                                                                               tttCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATAc                                         <  2:714242/140‑1 (MQ=255)

                                                                                                 tCGCCCGCAACAAATCCCCTCCGCTTGTGATGCCGTCCTAGCCGAGATCAATAAAATAGATACCGCCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGCGGTCAACATGCATTGCTGAAACGATACAt                                       <  2:3213756/140‑1 (MQ=255)

                                                                                                 tCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAt                                       >  2:929555/1‑140 (MQ=255)

                                                                                                 tCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAt                                       <  1:2037582/140‑1 (MQ=255)

                                                                                                  cGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACAtt                                      >  1:5062436/1‑140 (MQ=255)

                                                                                                       gCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCttt                                 >  2:3941711/1‑140 (MQ=255)

                                                                                                       gCACAAAATTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGACAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCttt                                 >  2:4430393/1‑140 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  2:4045919/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:2329684/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  2:4823961/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:3684655/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  2:2229232/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:1245921/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:494760/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  2:1197680/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  1:881904/140‑1 (MQ=255)

                                                                                                            aaaTTCCCGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGAGACATTTCTTTAACTg                            <  2:636950/140‑1 (MQ=255)

                                                                                                                 cccGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGc                       <  1:1419844/140‑1 (MQ=255)

                                                                                                                 cccGCCGGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGc                       <  1:4052081/140‑1 (MQ=255)

                                                                                                                  ccgccgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTg                            <  2:2734766/134‑1 (MQ=255)

                                                                                                                  ccgccgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCg                      >  1:754710/1‑140 (MQ=255)

                                                                                                                  ccgccgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCg                      >  1:3855499/1‑140 (MQ=255)

                                                                                                                    gccgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTa                    <  1:3584232/140‑1 (MQ=255)

                                                                                                                      cgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGTTACATTTCTTTAACTGTTGGCGTaga                  <  1:3087815/140‑1 (MQ=255)

                                                                                                                      cgGTGGTAATGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTaga                  <  1:3909926/140‑1 (MQ=255)

                                                                                                                              aggACCTCTTCGCCGACATCAATCATATAGATATCGACTTGGTTATCCATCGCACACCACGGGCAGTCAGTCAAATTAGCGGGGTAAACATGCATTGCTGAAGCGATATATTTCTTTACCCGTTGGCGTAGAGCGTCCAg          <  2:2500594/138‑1 (MQ=255)

                                                                                                                              aTGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCGAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGCTACGTTTCTTTAACTGTTGGCGTAGAGAGTCCAg          >  2:3543740/1‑140 (MQ=255)

                                                                                                                              aTGACCTCTTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAg          <  2:1930846/140‑1 (MQ=255)

                                                                                                                              aTGACCACTTCGCCGAGATCAATAAAATAGATACCACCTTGATTATCCAGCGCAAACAACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAg          <  2:1415945/140‑1 (MQ=255)

                                                                                                                                   ctctTCGCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCg     <  2:344697/140‑1 (MQ=255)

                                                                                                                                     cttcgCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCt   >  1:2625419/1‑140 (MQ=255)

                                                                                                                                      ttcgCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTAAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTACAGAGTCCAGTGCCCCTa  >  2:4907734/1‑140 (MQ=255)

CCTAAAGGAAGCGGCCTGCCAGTCGGTTGGAAATGATCGGGTAATGGCAGTTGCAATGCTGGCGGTGCTACTGACGCCATCACCATCGCCCAGACTTTCGCCAGCACAAAATTCCCGCCGGTGGTAATGACCTCTTCTCCGAGATCAATAAAATAGATAACACCTTGATTATCCAGCGCACACCACGGGCAGTCAGTCAAATGAGCGGGGTAAACATGCATTGCTGAAACGATACATTTCTTTAACTGTTGGCGTAGAGAGTCCAGTGCCGCTA  >  NZ_CP025268/2153865‑2154138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`