BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,193,631	T→C	A439A (GCA→GCG)	yehB ←	fimbrial biogenesis outer membrane usher protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,193,631	0	T	C	100.0%	668.2 / NA	204	A439A (GCA→GCG)	yehB	fimbrial biogenesis outer membrane usher protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (99/105); total (99/105)

Only 100 of 205 total aligned reads displayed.

TTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTG  >  NZ_CP025268/2193495‑2193767

ttttGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGgccgcc                                                                                                                                       <  2:3867722/140‑1 (MQ=255)

 tttGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCa                                                                                                                                      >  1:3978487/1‑140 (MQ=255)

       gCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCaa                                                                                                                                >  1:3173476/1‑140 (MQ=255)

              tCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGt                                                                                                                         <  1:4259930/140‑1 (MQ=255)

               cGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTc                                                                                                                        <  1:4480332/140‑1 (MQ=255)

                          taatCGGCAATGCCCTCGACATCATTTTAATCACGACGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGAGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCt                                                                                                             <  2:4129511/140‑1 (MQ=255)

                                gCAATGTCATAGGCATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACaaa                                                                                                       >  2:235865/1‑140 (MQ=255)

                                gCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACaaa                                                                                                       >  1:1558309/1‑140 (MQ=255)

                                gCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACaaa                                                                                                       >  1:3510564/1‑140 (MQ=255)

                                gCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACaaa                                                                                                       >  1:4696555/1‑140 (MQ=255)

                                 cAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAAt                                                                                                      <  2:4804066/140‑1 (MQ=255)

                                      tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCCAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATttgtt                                                                                                 >  1:2227560/1‑140 (MQ=255)

                                      tCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATttgtt                                                                                                 <  1:1857342/140‑1 (MQ=255)

                                         tAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTa                                                                                              <  1:3959940/140‑1 (MQ=255)

                                         tAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAAAGCGTCGACGTTTGGCTCACAAATTTGTTGTa                                                                                              >  2:2532103/1‑140 (MQ=255)

                                            aCATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGc                                                                                           <  1:2963251/140‑1 (MQ=255)

                                              aTCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGcca                                                                                         >  2:1053938/1‑138 (MQ=255)

                                              aTCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGCCGACGTTTGGCTCACAAATTTGTTGTAGGCaa                                                                                         >  2:796203/1‑140 (MQ=255)

                                              aTCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACCTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCTACGTTTGGCTCACAAATTTGTTGTAGGCaa                                                                                         >  1:2898879/1‑140 (MQ=255)

                                                ctttttCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGGTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  1:1353646/3‑140 (MQ=255)

                                                cattctcatcaCGGCGATAATTATCCTTATTCATTCCCCAAACGTGATCCTTAAATGCCCGGTAATCACGCGACGACCAACGACAGGCCGCCTGACCAAAACGCGTCGACGTTTGGCTCACACATTTGTTGTAGGCAAtt                                                                                       >  2:2874050/6‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTACATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTAGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  2:5074027/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCTAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  1:2462150/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCGAtt                                                                                       >  1:3724760/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  2:1363265/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  1:3452769/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  2:3223479/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  1:4102721/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  1:723880/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  1:477473/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  2:1816134/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  2:1746784/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  2:4283528/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  1:874059/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  1:259464/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  2:4736230/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCTTAATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAAtt                                                                                       >  1:1481687/1‑140 (MQ=255)

                                                cGTTTTCATCACGGCGATAATTATCCTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATCTGTTGTAGGCAAtt                                                                                       >  2:3211509/1‑140 (MQ=255)

                                                  ttttCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTg                                                                                     >  1:1621491/1‑140 (MQ=255)

                                                            ggCGATAATTATCTTTATTGTTTGCCCCAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAATTTTGTTGTAGTCAATTTGATAACTTTGc                                                                           <  1:1527791/140‑1 (MQ=255)

                                                            ggCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGTGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGGTGTAGGCAATTTGATAACTTTGc                                                                           >  1:761654/1‑140 (MQ=255)

                                                            ggCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGc                                                                           >  2:1541002/1‑140 (MQ=255)

                                                            ggCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGc                                                                           >  1:3845692/1‑140 (MQ=255)

                                                            gaccaTAATTATCTTTATTGTTTACCCAAACGTGATCGTTAAATGTCCTGTAATCACGCTACTAATAACGCCAGGCCCCTAGACCAAAACTCGTCTACGTTTGTCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGc                                                                           <  2:1157123/136‑1 (MQ=255)

                                                                 tAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGGCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTGGTTGTAGGCAATTTGATAACTTTGCCCGTc                                                                      <  1:1371099/140‑1 (MQ=255)

                                                                    ttATCTTTATTGTTTGCCCAAACGTGATCGTTACATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCaaa                                                                   <  2:2815397/140‑1 (MQ=255)

                                                                    ttATCGTTTTTGTTTGCCCAAACGCGCTCGCTAAATGTCCAGTAATCAAGCGACGAAAACCGCCAGGCCGCCAGACCAAACCGCGACAACGATTGGCACACAACTATGTTGTAGGAAATTTGATAACTTTGCCCGTCaaa                                                                   <  2:4415593/140‑1 (MQ=255)

                                                                      aTCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAaca                                                                 >  1:4615054/1‑140 (MQ=255)

                                                                        cTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAAcaca                                                               >  1:2481441/1‑140 (MQ=255)

                                                                         tttATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACAt                                                              <  1:2996669/140‑1 (MQ=255)

                                                                           tATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCg                                                            >  1:4209419/1‑140 (MQ=255)

                                                                           tATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCg                                                            <  1:5074175/140‑1 (MQ=255)

                                                                            agtGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGCAGGCAATTTGATAGCTTTGTCCGTCAAACACATCGc                                                           >  1:2848513/3‑140 (MQ=255)

                                                                            aTTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCATGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGc                                                           >  2:729357/1‑140 (MQ=255)

                                                                              tGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCg                                                         <  1:191370/140‑1 (MQ=255)

                                                                              tGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCg                                                         <  1:3091614/140‑1 (MQ=255)

                                                                                tttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                       <  1:3381698/140‑1 (MQ=255)

                                                                                tttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                       <  2:2481232/140‑1 (MQ=255)

                                                                                tttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                       <  1:1626782/140‑1 (MQ=255)

                                                                                tttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGtt                                                       <  1:1092111/140‑1 (MQ=255)

                                                                                tttGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCAGtt                                                       <  1:235865/140‑1 (MQ=255)

                                                                                  tccccAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGTCTCACACCTTTGTTTTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGt                                                     <  2:581747/138‑1 (MQ=255)

                                                                                  tGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGt                                                     >  1:1176468/1‑140 (MQ=255)

                                                                                        aaCGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGtt                                               <  1:4340788/140‑1 (MQ=255)

                                                                                        aaCGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGtt                                               <  1:4171383/140‑1 (MQ=255)

                                                                                              aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGGTAAGTTTGCGCGTCAAACACATCGCCGTTGTCTTGTTTACTAt                                         >  2:1909064/1‑140 (MQ=255)

                                                                                              aTCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTAt                                         >  2:4554768/1‑140 (MQ=255)

                                                                                               tagTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAACCGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCACACACATCGCCGTTGTCTTGTTTACTATg                                        <  2:448919/138‑1 (MQ=255)

                                                                                               tCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATg                                        <  1:1483771/140‑1 (MQ=255)

                                                                                               tCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATg                                        <  2:510619/140‑1 (MQ=255)

                                                                                               tCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATg                                        <  1:4956129/140‑1 (MQ=255)

                                                                                               tCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGGTGTCTTGTTTACTATg                                        <  1:1866602/140‑1 (MQ=255)

                                                                                                  ttAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGa                                     <  2:4656333/140‑1 (MQ=255)

                                                                                                   tAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGAc                                    >  1:2226214/1‑140 (MQ=255)

                                                                                                   tAAATGTCCGGTAATCACGCGACGAATAAACCCAGGCCGACAGACCAAAACGCGTCGACATTTGGCTCACAAATGTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGaa                                    >  2:2111987/1‑139 (MQ=255)

                                                                                                           cGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGc                            >  2:741688/1‑140 (MQ=255)

                                                                                                           cGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGc                            <  1:2731931/140‑1 (MQ=255)

                                                                                                           cGGTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGc                            >  2:4155530/1‑140 (MQ=255)

                                                                                                             gTAATCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCAt                          >  2:3477272/1‑140 (MQ=255)

                                                                                                                 tCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGAc                      <  1:53404/140‑1 (MQ=255)

                                                                                                                 tCACGCGACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTGGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGAc                      <  2:3920864/140‑1 (MQ=255)

                                                                                                                     gcgACGAATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGaa                  >  1:4722864/1‑140 (MQ=255)

                                                                                                                      cgacgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGaaa                 <  1:4705046/140‑1 (MQ=255)

                                                                                                                      cgacgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGaaa                 <  2:1481687/140‑1 (MQ=255)

                                                                                                                      cgacgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGaaa                 <  1:1746784/140‑1 (MQ=255)

                                                                                                                      cgacgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGaaa                 <  1:203878/140‑1 (MQ=255)

                                                                                                                        acgaATAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCGTGTTTACTATGCGACTTAGTGGCATCGACGGAAATg               >  1:5035886/1‑140 (MQ=255)

                                                                                                                           aaTAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCa            <  1:642097/140‑1 (MQ=255)

                                                                                                                            aTAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCAc           <  1:2646772/140‑1 (MQ=255)

                                                                                                                             tAACGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCAcc          <  2:3390544/140‑1 (MQ=255)

                                                                                                                             gaaCGCCAGGCCGCCAGACCAAAACGCGTCGACGGTTGGCTAACAAATTTGACGTAGGCAAATTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTTGCATCGACGGAAATGGCAcc          >  2:294619/2‑140 (MQ=255)

                                                                                                                               aCGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCaa        <  1:3860848/140‑1 (MQ=255)

                                                                                                                               aCGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCaa        >  1:4717532/1‑140 (MQ=255)

                                                                                                                               aCGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCaa        <  1:3370448/140‑1 (MQ=255)

                                                                                                                                cGCCAGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCCCAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAAt       <  2:2844154/140‑1 (MQ=255)

                                                                                                                                    aGGCCGCCTGTCCAAAACGCGACGACGTTTGGCGCACAAATTTGTTGTAGGCAATCAGATAACTTTGCCCGTCAAACACATCGCCGTTGTCGTGTTTACTATGCGACTTAGTGGCATCGACGGCAATGGCACCAATGCgt   <  2:3736318/140‑1 (MQ=255)

                                                                                                                                    aGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCgt   <  1:3123604/140‑1 (MQ=255)

                                                                                                                                    aGGCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCgt   <  1:3580930/140‑1 (MQ=255)

                                                                                                                                     ggCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCTGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCgtg  >  2:1339441/1‑140 (MQ=255)

                                                                                                                                     ggCCGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCgtg  >  2:2632045/1‑140 (MQ=255)

TTTTGCGGCCAAAATCGTTCTGGTAATAATCGGCAATGTCATAGACATCGTTTTCATCACGGCGATAATTATCTTTATTGTTTGCCCAAACGTGATCGTTAAATGTCCGGTAATCACGCGACGAATAACGCCAGGCTGCCAGACCAAAACGCGTCGACGTTTGGCTCACAAATTTGTTGTAGGCAATTTGATAACTTTGCCCGTCAAACACATCGCCGTTGTCTTGTTTACTATGCGACTTAGTGGCATCGACGGAAATGGCACCAATGCGTG  >  NZ_CP025268/2193495‑2193767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`