BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,280,334	A→G	342 (TAA→TAG)	yejE →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,280,334	0	A	G	99.6%	820.0 / NA	249	342 (TAA→TAG)	yejE	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (138/109); total (138/111)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

TGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGG  >  NZ_CP025268/2280198‑2280469

tgTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCAt                                                                                                                                      <  1:4551128/140‑1 (MQ=255)

tgTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCAt                                                                                                                                      <  1:4166779/140‑1 (MQ=255)

    aCAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTGCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGGGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACg                                                                                                                                  >  2:4091777/1‑140 (MQ=255)

      aaGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCa                                                                                                                                <  1:1044792/140‑1 (MQ=255)

      aaGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCa                                                                                                                                <  2:3919049/140‑1 (MQ=255)

        agcaaaaaTAACCTTCAGTCCCCGTGGCTTTGGATCACCGCCTTCTTGTCGGTGTCGATATTATTGTCTTTGCTCATCCTTATTGGTGAAGCCGTCCGCGACGCATTTGAGCCCAATAAGGCGGTGTAGCATGACGCaaa                                                                                                                              <  2:3202944/137‑1 (MQ=255)

         ggAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGCCGGTGGCGCGATTATTGTCTTTGCTGAGTTTTATTGGTGAAGCCTGCCGCGACGCATTTGATCCTAATAAGGCGGTGGAGCATGACGCAAAc                                                                                                                             >  2:2331857/1‑140 (MQ=255)

            aaaaTAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAActct                                                                                                                          >  2:4433706/1‑140 (MQ=255)

                   ccTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCg                                                                                                                   >  1:97645/1‑140 (MQ=255)

                     ttCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGGTATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGAt                                                                                                                 >  2:3836669/1‑140 (MQ=255)

                       cAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGAttt                                                                                                               >  1:3900680/1‑139 (MQ=255)

                             ccGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAAtt                                                                                                         <  2:4884236/140‑1 (MQ=255)

                             ccGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAAtt                                                                                                         >  2:3622257/1‑140 (MQ=255)

                              cGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAAttt                                                                                                        >  2:4628901/1‑140 (MQ=255)

                               gTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGCTATTATTGTCTTTGCTGATCTTTCTTGGTGAAGCCGTCCGCGACGCATCTGATCCTAATAAGGCGGCGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTg                                                                                                       >  2:2274073/1‑140 (MQ=255)

                               gTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTg                                                                                                       >  1:3486869/1‑140 (MQ=255)

                               gTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTg                                                                                                       >  1:1168863/1‑140 (MQ=255)

                                    ttGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGCCGCATTTGATCCTAATAAGCCGGTGTAGCATGACGTAAACTCTGTTAGCGATTGAAAATTTGTCGGt                                                                                                  <  2:4984987/140‑1 (MQ=255)

                                    ttGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGt                                                                                                  >  2:1396724/1‑140 (MQ=255)

                                    ttGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGt                                                                                                  >  1:599353/1‑140 (MQ=255)

                                            aCCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTc                                                                                          >  2:2490037/1‑140 (MQ=255)

                                              cGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGc                                                                                        >  1:1865664/1‑140 (MQ=255)

                                              cGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGc                                                                                        <  2:1318213/140‑1 (MQ=255)

                                                ccTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCa                                                                                      <  2:4102572/140‑1 (MQ=255)

                                                    cttGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAg                                                                                  <  2:1447633/140‑1 (MQ=255)

                                                       gTCGGTGGCGCTATTATTGTCTTTGCTGATCTTTATTGTTGAAGCCTGCCGCGCCGCATTTGATCCTAATCAGGCGGTGTAGCATTACGCAAACTCTCTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                               <  2:1053940/140‑1 (MQ=255)

                                                       gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                               <  1:3538797/140‑1 (MQ=255)

                                                       gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                               <  2:1539728/140‑1 (MQ=255)

                                                       gTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCaa                                                                               <  1:1280686/140‑1 (MQ=255)

                                                         cggcggCCATATTTTTGTCTTTGCTTTTCTTTATTGCTGACGCCGTCCGCGACGCATTTGATCCTAATTATGCGGTGTAGCATGACGCAACCTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                             <  2:1656193/140‑1 (MQ=255)

                                                         cGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                             <  1:2790275/140‑1 (MQ=255)

                                                          ggTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAcc                                                                            <  2:2600100/140‑1 (MQ=255)

                                                          ggTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAcc                                                                            <  1:4915233/140‑1 (MQ=255)

                                                           gTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcg                                                                           >  2:969858/1‑140 (MQ=255)

                                                            tttatatatTATTCTCTTTGCTGATCTTTATTGTTGACGCCTTCCGCGACGCATTTGATCCTAATATGGCGGTGTAGCATGACGCACACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                          <  2:146011/135‑1 (MQ=255)

                                                            tGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGGAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                          <  2:3668590/140‑1 (MQ=255)

                                                            tGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                          <  1:2705446/140‑1 (MQ=255)

                                                            tGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                          <  1:3033654/140‑1 (MQ=255)

                                                            tGGCGATATGATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATGTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGACAATGTGTCGGTGGGTTTTCGCCATCAGCAAACcgt                                                                          <  1:3002910/140‑1 (MQ=255)

                                                              gCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                             >  2:3261575/1‑135 (MQ=255)

                                                              gCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtac                                                                        <  1:406586/140‑1 (MQ=255)

                                                               cGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacg                                                                       <  2:761416/140‑1 (MQ=255)

                                                               cGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacg                                                                       >  1:4003005/1‑140 (MQ=255)

                                                                gATATTATTTTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgt                                                                      <  2:3145236/140‑1 (MQ=255)

                                                                 atatTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAACAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACcgtacgta                                                                     <  1:2844958/140‑1 (MQ=255)

                                                                   attattGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa                                                                   <  1:3826533/140‑1 (MQ=255)

                                                                   attattGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCAACGCATTTGATCCTAATAAGGCGGTGTAGCATAACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACa                                                                   <  2:4232169/140‑1 (MQ=255)

                                                                      attGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACagta                                                                <  1:4607567/140‑1 (MQ=255)

                                                                        tGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACagtagt                                                              >  1:4966298/1‑140 (MQ=255)

                                                                        tGTCTGTGCGGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGAGCTGCATGACGCAATCTCTGTTGGCGAGTGACAATTTGTCGGTCGCATTTGGCGCTCCGCACGCCGTACGTGCagtggt                                                              >  2:182826/1‑140 (MQ=255)

                                                                            tttGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAat                                                          >  2:1356580/1‑140 (MQ=255)

                                                                            gttGCTGAGCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGAAGCATAGCCAAAACTCTGTTAGCGGTTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACGGTAGGAACGTGAGTCAat                                                          >  2:907740/2‑140 (MQ=255)

                                                                             ttGCTGATCTTTATCGGTGAAGCCGTCCGCGACGCATTTGATCCTACTAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatg                                                         <  2:1711254/140‑1 (MQ=255)

                                                                              tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                        >  1:1996777/1‑140 (MQ=255)

                                                                              tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                        >  2:4625129/1‑140 (MQ=255)

                                                                              tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                        >  1:1531907/1‑140 (MQ=255)

                                                                              tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                        <  2:1971673/140‑1 (MQ=255)

                                                                              tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                        >  1:5061560/1‑140 (MQ=255)

                                                                              tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatga                                                        >  1:4124302/1‑140 (MQ=255)

                                                                              tGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTAGCCATCAGCAAACCGTAGGTACAGTAGTAAatga                                                        >  2:1508317/1‑140 (MQ=255)

                                                                              tGCTGAGCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGGACAGTAGTCAatga                                                        >  2:185216/1‑140 (MQ=255)

                                                                                ttGATGTTTTTTGGTGTAGCCGGCCCCGACGCATTTGATCCTAATAAGGCGGTGTGGCATGACGCGAGCTCTCTTACCGATTGAAGATTTGTCGGTGGGTTTTCGCCATCAGCAAACGGTGCGTACAGTAGTCAttgatg                                                      <  2:4808525/139‑1 (MQ=255)

                                                                                gtGATCTTTATTCGTTAAGCCGTGCGCCACGCCTTTCATCCTTATTAGGCGGTGTATCATGACTCAACCTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAatgatg                                                      <  2:5012502/139‑1 (MQ=255)

                                                                                   aTCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGttt                                                   <  1:1985311/140‑1 (MQ=255)

                                                                                   aTCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGttt                                                   <  1:2214280/140‑1 (MQ=255)

                                                                                   aTCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTCTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGttt                                                   <  1:381765/140‑1 (MQ=255)

                                                                                    tcgttATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGACAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTc                                                  >  2:4142321/4‑140 (MQ=255)

                                                                                    tCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAAGTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGGAGTCAATGATGtttt                                                  >  2:5101575/1‑139 (MQ=255)

                                                                                        tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTa                                              <  1:3740277/140‑1 (MQ=255)

                                                                                        tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTa                                              >  1:4683784/1‑140 (MQ=255)

                                                                                        tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTa                                              <  1:2610322/140‑1 (MQ=255)

                                                                                        tATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTa                                              <  1:2077493/140‑1 (MQ=255)

                                                                                             gTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGAt                                         >  1:1757427/1‑140 (MQ=255)

                                                                                             gTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGAt                                         >  2:2728668/1‑140 (MQ=255)

                                                                                             gTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGAt                                         >  2:3333336/1‑140 (MQ=255)

                                                                                             gTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGAt                                         >  1:2431157/1‑140 (MQ=255)

                                                                                              tGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGAtt                                        >  2:1621585/1‑140 (MQ=255)

                                                                                               gAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGTTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTg                                       >  1:5018125/1‑140 (MQ=255)

                                                                                                aaGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGa                                      >  2:4744204/1‑140 (MQ=255)

                                                                                                  gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                             >  1:4708361/1‑99 (MQ=255)

                                                                                                  gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAAc                                                                             <  2:4708361/99‑1 (MQ=255)

                                                                                                  gCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAgg                                    >  2:2159305/1‑140 (MQ=255)

                                                                                                   ccGTCCGCGACCCGTTTTATCCTAGTAACGCGGTGTCGCATGTCCCCGACTCTGTTAGCGATTGAAAATTTGTCGGTGGGGTTTGGACAGCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGc                                   <  2:3048998/140‑1 (MQ=255)

                                                                                                             cGCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcg                         >  1:3842487/1‑140 (MQ=255)

                                                                                                              gCATTTGATCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgc                        >  2:1561852/1‑140 (MQ=255)

                                                                                                                      tCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctggcgctg                <  1:4762605/140‑1 (MQ=255)

                                                                                                                      tCCTAATAAGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAAcgctggcgctg                <  2:2462464/140‑1 (MQ=255)

                                                                                                                         taataaGGCGGTGGAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtg             <  1:4357938/140‑1 (MQ=255)

                                                                                                                          aataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCtggtgg            >  2:2025906/1‑140 (MQ=255)

                                                                                                                           ataaGGCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTggtggg           <  1:4400417/140‑1 (MQ=255)

                                                                                                                               ggCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAg       >  1:1359686/1‑140 (MQ=255)

                                                                                                                                gCGGTGTAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGt      >  1:4466550/1‑140 (MQ=255)

                                                                                                                                    tgtAGCATGACGCAAACTCTGTTAGCGATTGCCAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTCCAGCTTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg  >  1:822319/1‑140 (MQ=255)

                                                                                                                                    tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGGGTCAgg  >  2:2059546/1‑140 (MQ=255)

                                                                                                                                    tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg  >  1:2323520/1‑140 (MQ=255)

                                                                                                                                    tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg  >  2:3370803/1‑140 (MQ=255)

                                                                                                                                    tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg  >  1:3600694/1‑140 (MQ=255)

                                                                                                                                    tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg  >  1:902025/1‑140 (MQ=255)

                                                                                                                                    tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAgg  >  1:4262259/1‑140 (MQ=255)

                                                                                                                                    tgtAGCATGACGCAAACTCTGTTAGCGATTGAAAAGTAGTCGGTTGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCCATACAGATTTAGGCTGGCGAAACGCTGGCGCTGGGGGGGGAGGCAgg  >  2:4320911/1‑140 (MQ=255)

TGTTACAAGGGAAAAATAACCTTCAGGCCCCGTGGCTTGGGATCACCGCCTTCTTGTCGGTGGCGATATTATTGTCTTTGCTGATCTTTATTGGTGAAGCCGTCCGCGACGCATTTGATCCTAATAAGGCGGTGTAACATGACGCAAACTCTGTTAGCGATTGAAAATTTGTCGGTGGGTTTTCGCCATCAGCAAACCGTACGTACAGTAGTCAATGATGTTTCACTACAGATTGAGGCTGGCGAAACGCTGGCGCTGGTGGGTGAGTCAGG  >  NZ_CP025268/2280198‑2280469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`