BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,415,086	G→C	T101S (ACC→AGC)	yfbS ←	SLC13 family permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,415,086	0	G	C	99.7%	1001.7 / NA	301	T101S (ACC→AGC)	yfbS	SLC13 family permease
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (124/176); minor base A (0/1); total (124/177)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 301 total aligned reads displayed.

AATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGT  >  NZ_CP025268/2414950‑2415221

  tCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAACCCCGCGACGCTCAGc                                                                                                                                     >  1:60764/1‑140 (MQ=255)

  ccAGCCCGGCAAACCTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGc                                                                                                                                     <  2:1170415/139‑1 (MQ=255)

      cccGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATca                                                                                                                                 <  1:2633332/140‑1 (MQ=255)

        cGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATcaac                                                                                                                               <  1:4946791/140‑1 (MQ=255)

           cAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑aaa                                                                                                                           >  2:1332847/1‑140 (MQ=255)

                ttcaaCGGCATCATCAGAGGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCaa                                                                                                                      <  2:2838219/137‑1 (MQ=255)

                      ggCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACAtttt                                                                                                                <  1:1429689/140‑1 (MQ=255)

                      ggCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACAtttt                                                                                                                <  1:3977571/140‑1 (MQ=255)

                        catcatCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGa                                                                                                              <  2:962463/140‑1 (MQ=255)

                          tcatcaGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGAtt                                                                                                            <  1:37420/140‑1 (MQ=255)

                          tcatcaGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGAtt                                                                                                            >  1:3786359/1‑140 (MQ=255)

                                  cGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTAtt                                                                                                    >  1:2748929/1‑140 (MQ=255)

                                    tGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGc                                                                                                  >  1:3396778/1‑140 (MQ=255)

                                      aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGccc                                                                                                >  2:1024960/1‑140 (MQ=255)

                                      aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGccc                                                                                                >  1:2908271/1‑140 (MQ=255)

                                      aCGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTAGATTTCACTATTGccc                                                                                                >  1:2835898/1‑140 (MQ=255)

                                       cGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCg                                                                                               <  1:2494177/140‑1 (MQ=255)

                                          cGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATGTTGACTTCACTATTGCCCGCaa                                                                                            <  1:4054487/140‑1 (MQ=255)

                                            aCGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACt                                                                                          <  2:5071882/140‑1 (MQ=255)

                                            aCGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACt                                                                                          <  2:4160301/140‑1 (MQ=255)

                                                 tgaaTACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                     <  2:279400/137‑1 (MQ=255)

                                                 tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGCTAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                     <  2:2961236/140‑1 (MQ=255)

                                                 tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                     <  1:3960391/140‑1 (MQ=255)

                                                 tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                     <  1:3868465/140‑1 (MQ=255)

                                                 tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                     <  2:904755/140‑1 (MQ=255)

                                                 tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                     <  1:3523659/140‑1 (MQ=255)

                                                 tGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGAc                                                                                     <  2:3309040/140‑1 (MQ=255)

                                                       cGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCATCATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCa                                                                               <  1:2776441/140‑1 (MQ=255)

                                                        tcATGGCAACGCTTAACACCACCGGAATAAAGATAGCGGCAACGCCGGTTGAACTCATAAACACGCCAAGCCCCGCCACGCTCAGCATCAAC‑AAAACCAACATTTTGAGTTCACTATTTCCCGCAACTTCGACCAGCCAt                                                                              >  2:202864/2‑140 (MQ=255)

                                                          aTGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGt                                                                            <  2:1127680/140‑1 (MQ=255)

                                                          aTGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGt                                                                            <  2:351686/140‑1 (MQ=255)

                                                            ggCAACGTTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGCACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTACACTATTGCCCTCCACTTTGACCAGCCATGTTc                                                                          >  2:4046599/1‑140 (MQ=255)

                                                            ggCAACGCTTAGCACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAACAAAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGtt                                                                           >  1:4221422/1‑140 (MQ=255)

                                                                 cGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCAtt                                                                     >  1:348385/1‑140 (MQ=255)

                                                                  gCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTa                                                                    >  2:4898267/1‑140 (MQ=255)

                                                                  gCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTa                                                                    >  2:4555709/1‑140 (MQ=255)

                                                                   cTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAACCCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTAc                                                                   <  1:4600492/140‑1 (MQ=255)

                                                                   cTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTAc                                                                   <  1:1688383/140‑1 (MQ=255)

                                                                   cTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTAc                                                                   <  1:4801271/140‑1 (MQ=255)

                                                                   cTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTAc                                                                   <  1:1572305/140‑1 (MQ=255)

                                                                      aaCACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGt                                                                >  1:1992150/1‑140 (MQ=255)

                                                                         accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                             <  1:3025642/140‑1 (MQ=255)

                                                                         accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                             <  1:710936/140‑1 (MQ=255)

                                                                         accacGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGc                                                             <  2:1869302/140‑1 (MQ=255)

                                                                           aacGGGAATAAAGATATCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTGTGACCAGCCATGTTCGCATTCCGTTGGCaa                                                           >  2:360980/2‑140 (MQ=255)

                                                                            acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACTCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                          >  1:2168400/1‑140 (MQ=255)

                                                                            acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                          >  1:1472341/1‑140 (MQ=255)

                                                                            acGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAac                                                          >  2:2539079/1‑140 (MQ=255)

                                                                             cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                         >  2:2264994/1‑140 (MQ=255)

                                                                             cGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAaca                                                         >  1:4401896/1‑140 (MQ=255)

                                                                                  aTAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGt                                                    >  1:4448287/1‑140 (MQ=255)

                                                                                  aTAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGt                                                    <  1:899079/140‑1 (MQ=255)

                                                                                   tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                   >  1:4601979/1‑140 (MQ=255)

                                                                                   tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                   <  1:2318903/140‑1 (MQ=255)

                                                                                   tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                   <  1:1612781/140‑1 (MQ=255)

                                                                                   tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                   <  2:768811/140‑1 (MQ=255)

                                                                                   tAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTa                                                   <  2:1622423/140‑1 (MQ=255)

                                                                                     aaGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACg                                                 >  1:4306329/1‑140 (MQ=255)

                                                                                          aGCGACAACGCCGGTTGAACTCATAAACGCGCCACGCCCCCCAACGCTCAACATCAAC‑AACCACAACATTTTCATTTCACTATTGCCCGCAACTTCGACCAGCCAAGCTCCCATTACGCTCGCACCCCCGGTACGGACCa                                            >  2:3675173/1‑140 (MQ=255)

                                                                                          aGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCa                                            >  2:2160474/1‑140 (MQ=255)

                                                                                           gCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaa                                           >  2:4325882/1‑140 (MQ=255)

                                                                                            cGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCaaa                                          >  1:2067843/1‑140 (MQ=255)

                                                                                               cAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCa                                       <  2:4733135/140‑1 (MQ=255)

                                                                                                aaCGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCAt                                      >  1:520068/1‑140 (MQ=255)

                                                                                                  cGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCg                                    >  1:3911258/1‑140 (MQ=255)

                                                                                                  cGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCg                                    >  1:3352323/1‑140 (MQ=255)

                                                                                                  cGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACGTTGACCAGCAAGGTTCCCATTAGGGGGGCAACAGCGGTACCAACGAAACCATCg                                    >  2:194978/1‑140 (MQ=255)

                                                                                                  cGCCGGTTGAACGCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCg                                    >  2:5052404/1‑140 (MQ=255)

                                                                                                   gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCACCATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                   <  1:501090/140‑1 (MQ=255)

                                                                                                   gCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGc                                   <  2:3350717/140‑1 (MQ=255)

                                                                                                     cgcttcaaCTCATAACCGCGCCAAGCCCCGCGACGCACAGCATCAAC‑AAAACCAACCTGTTGATTTCACTATTGCCCGCAACTTTCACCAGCCATGTTCCCATTACGGTGGCAACACCGATACGGACCAAACCATCGCCa                                 <  2:2433198/134‑1 (MQ=255)

                                                                                                      ggTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCaa                                >  1:3272236/1‑140 (MQ=255)

                                                                                                       gTTGAACTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCaat                               >  1:1953184/1‑140 (MQ=255)

                                                                                                            aCTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAAtaataa                          >  2:586552/1‑140 (MQ=255)

                                                                                                             cTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATaaa                         <  1:1224944/140‑1 (MQ=255)

                                                                                                             cTCATAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATaaa                         >  1:2145105/1‑140 (MQ=255)

                                                                                                                aTAAACGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACaa                      >  1:4740576/1‑140 (MQ=255)

                                                                                                                  aaaCGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAgg                    >  1:3619427/1‑140 (MQ=255)

                                                                                                                  aaaCGCGCCAAGCCCCGCCACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAgg                    <  2:2597045/140‑1 (MQ=255)

                                                                                                                    aCGCGCCCAGCCCCGCGACGCTCAGCACCAAC‑AAAACCAACATTTTGCTTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                  <  2:3241028/140‑1 (MQ=255)

                                                                                                                    aCGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                  <  2:1736480/140‑1 (MQ=255)

                                                                                                                    aCGCGCCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAggcg                  <  2:4249065/140‑1 (MQ=255)

                                                                                                                        gcCAAGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCaa              >  2:3176986/1‑140 (MQ=255)

                                                                                                                         ccAAGCCCCGCGACGCGCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAAt             <  1:577116/140‑1 (MQ=255)

                                                                                                                            aGCCCCGCGACGCTCAGCATCAAC‑AGAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATccg          >  2:4571035/1‑138 (MQ=255)

                                                                                                                            aGCCCCGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAAACAg          >  2:211962/1‑140 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTTTTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTAGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa        <  2:4402448/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa        <  2:3837463/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa        <  1:952269/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa        <  1:822464/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa        <  1:648440/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa        <  2:236818/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa        <  1:1747364/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa        <  1:2459097/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa        <  1:2442210/140‑1 (MQ=255)

                                                                                                                              ccccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGa        <  2:2685169/140‑1 (MQ=255)

                                                                                                                               ccggcggcgATCAGCATCAAC‑ACAACCAACCTTTTGATTTCACTGTTGCCCGCAACTTTGACCAGCCAGGTTACCATGACGGTGGCAACACCGGTACGGACCAAAACATCGCGAATAATATACAAGGCGGCAAGCAGGAc       <  2:1250976/137‑1 (MQ=255)

                                                                                                                               cccGCGTCGCTCACGATATAC‑ACAACCAACATTGTGATTTCCCCCTTGCCGGCGACTTTGGCCAGCGATGTTCCCATGACGGTGGCAACAGCGGTACGGACAAGACCATCGCCAATAATAAACAAGGCGGCAATCAGGAc       <  2:3929829/140‑1 (MQ=255)

                                                                                                                                ccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACa      <  1:3141183/140‑1 (MQ=255)

                                                                                                                                ccGCGACGCTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACa      <  1:1027909/140‑1 (MQ=255)

AATCAGCCCGGCAAAACTTAACGGCATCATCAGACGTGACGGCGACGTTTGCATACGCATGGCAACGCTTAACACCACGGGAATAAAGATAGCGACAACGCCGGTTGAACTCATAAACGCGCCAAGCCCCGCGACGGTCAGCATCAAC‑AAAACCAACATTTTGATTTCACTATTGCCCGCAACTTTGACCAGCCATGTTCCCATTACGGTGGCAACACCGGTACGGACCAAACCATCGCCAATAATAAACAAGGCGGCAATCAGGACAACGT  >  NZ_CP025268/2414950‑2415221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`