BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	247,291	A→G	250 (TAA→TAG)	yafL →	C40 family peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	247,291	0	A	G	99.6%	746.8 / NA	225	250 (TAA→TAG)	yafL	C40 family peptidase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (93/131); total (93/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 225 total aligned reads displayed.

TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGA‑C‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTT  >  NZ_CP025268/247155‑247429

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                           >  2:2604284/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                           >  1:4919749/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                           >  1:1286598/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                           >  1:3712948/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                           >  1:349332/1‑140 (MQ=255)

tgtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑c                                                                                                                                           >  1:92479/1‑140 (MQ=255)

 gtATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑g                                                                                                                                         >  1:4450308/1‑140 (MQ=255)

  tATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑gg                                                                                                                                        >  1:3308355/1‑139 (MQ=255)

      tGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑C‑ggataa                                                                                                                                    <  1:2515454/140‑6 (MQ=255)

            attggCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGAGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGtt                                                                                                                              <  2:4020088/137‑1 (MQ=255)

            aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGtt                                                                                                                              >  2:1827032/1‑140 (MQ=255)

            aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTGTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGtt                                                                                                                              >  1:4240432/1‑140 (MQ=255)

            aTGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATATTTTGGGCGCGCGCAGGATTTTGACGGAATAGACGATTTTGTAGGA‑CGG‑ATAAGGCGtt                                                                                                                              >  1:2812568/1‑140 (MQ=255)

               ggCAATTTATCGAGGCGCCACGTACTGGCGAAACGATTAGGATAAGCCGATTAGCCGAAGCTTTTTGGCAGGTCCTATTTTTGGGTGCGCGCAGGATTGGTACGGAAGAGGCGATTTTGTAGGAGG‑G‑ATACGGCGTTTAc                                                                                                                           >  2:1394244/1‑140 (MQ=255)

                gCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTAcg                                                                                                                          <  1:357709/140‑1 (MQ=255)

                   atctATCGCGTCCACACGTACCGGCGAAACCATTCCGATAAGCCGATTAGCAGAACCTTTCTGGCAGCACCATTTTTTGCGCGCGCGCAGGATTCTGACGGAAGAGCCCATTTTGTCGGA‑CAG‑ATAAGGCGTTTAcgccg                                                                                                                       <  2:2966141/137‑1 (MQ=255)

                     ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCa                                                                                                                     <  1:2999567/140‑1 (MQ=255)

                     ttATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACATTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCa                                                                                                                     <  1:2743536/140‑1 (MQ=255)

                       ttCGAGTCGCCACGTACCGGCGAACCCATTCGGATACGCCGATTAGCCGAACCCTTCTGGCAGGACCATTTTTTGGGAGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATc                                                                                                                   <  2:238535/139‑1 (MQ=38)

                       aTCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGATACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGGCGCAtt                                                                                                                   >  2:278000/1‑139 (MQ=255)

                         cGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGACCCTTTCTGGCAGGACCATTTTTTGTGCGCGCGCAGCATTTTGACGGAAGAGACGATGTTGTAGGA‑CGG‑ATAAGGCGTTTACGGCGCATCCg                                                                                                                 >  2:3724670/1‑140 (MQ=255)

                         cGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCg                                                                                                                 >  1:4774054/1‑140 (MQ=255)

                         cGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTCTACGCCGCATCCg                                                                                                                 >  2:4379822/1‑140 (MQ=255)

                          gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAGCCTTTCTGGCAGGACCATTTGTTGGGCGCGTGCAGGATTTTGGCGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                                <  2:4690310/140‑1 (MQ=38)

                          gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                                <  1:2147423/140‑1 (MQ=255)

                          gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                                <  1:4173855/140‑1 (MQ=255)

                          gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                                <  1:5043566/140‑1 (MQ=255)

                          gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                                <  2:1948083/140‑1 (MQ=255)

                          gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                                <  1:327735/140‑1 (MQ=255)

                          gAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCgg                                                                                                                <  1:2687229/140‑1 (MQ=255)

                             tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                             <  2:2078254/140‑1 (MQ=255)

                             tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAg                                                                                                             <  1:1995090/140‑1 (MQ=255)

                             tCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCATTTACGCCGCATCCGGCAg                                                                                                             <  2:4480963/140‑1 (MQ=255)

                                      aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                    <  2:3209875/140‑1 (MQ=255)

                                      aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                    <  1:743681/140‑1 (MQ=255)

                                      aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                    >  1:1938655/1‑140 (MQ=255)

                                      aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                    >  2:3168560/1‑140 (MQ=255)

                                      aCCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGCAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCa                                                                                                    <  2:4662244/140‑1 (MQ=255)

                                       ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                                   >  2:2648000/1‑140 (MQ=255)

                                       ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                                   >  2:1495085/1‑140 (MQ=255)

                                       ccGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAg                                                                                                   <  2:4818754/140‑1 (MQ=255)

                                         ggCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGt                                                                                                 <  2:699650/140‑1 (MQ=255)

                                          gCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTg                                                                                                >  1:3777390/1‑140 (MQ=255)

                                            gAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGcc                                                                                              <  1:4602473/140‑1 (MQ=255)

                                             aaaCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCt                                                                                             >  1:1365876/1‑140 (MQ=255)

                                               aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa                                                                                           >  2:782295/1‑140 (MQ=255)

                                               aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa                                                                                           >  2:3243843/1‑140 (MQ=255)

                                               aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa                                                                                           <  2:2866720/140‑1 (MQ=255)

                                               aCCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGa                                                                                           >  1:1030999/1‑140 (MQ=255)

                                                 cATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATg                                                                                         <  2:3282297/140‑1 (MQ=255)

                                                    tCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGa                                                                                      >  2:3463045/1‑140 (MQ=255)

                                                      ggATAAGCCGATTGGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACg                                                                                    <  1:618368/140‑1 (MQ=255)

                                                      ggATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACg                                                                                    <  1:2255537/140‑1 (MQ=255)

                                                        aTAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCt                                                                                  <  1:429828/140‑1 (MQ=255)

                                                         tAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTg                                                                                 >  1:3268692/1‑140 (MQ=255)

                                                           ggCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                               <  1:4507164/139‑1 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                               <  1:1554214/140‑1 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                               <  1:3827165/140‑1 (MQ=255)

                                                           aGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                               <  1:3925554/140‑1 (MQ=255)

                                                           aGCCGATTAGCCCAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGgc                                                                               >  2:5027540/1‑140 (MQ=39)

                                                           aGCAGATTAGCCGAAGCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGCA‑CGG‑ATAATGCGTTTACGCCGCGTCCGTCAGTTGTTCGCAGGTGCCTGATGCGACGCTGgc                                                                               <  2:1059337/140‑1 (MQ=37)

                                                             ccGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTCGGA‑CGG‑ATAAGGCGTTTCCGCCGCATCCGGCAGTTGTCCGCAGGTGCCTGATGCGACGCTGgcgc                                                                             <  2:2424243/140‑1 (MQ=38)

                                                              cGATTAGCCGAACCTGTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGcgcg                                                                            <  2:412049/140‑1 (MQ=255)

                                                               gATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                           <  2:253500/140‑1 (MQ=255)

                                                               gATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGt                                                                           <  2:5060019/140‑1 (MQ=255)

                                                                aTTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTc                                                                          >  2:3856904/1‑140 (MQ=255)

                                                                aTTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTc                                                                          >  2:1494803/1‑140 (MQ=255)

                                                                 ttAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCt                                                                         >  2:4329703/1‑140 (MQ=255)

                                                                  tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGGAGGATTTTGACGGAAGAGTCTTTTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                        >  2:1345154/1‑140 (MQ=25)

                                                                  tAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCtt                                                                        >  2:2667173/1‑140 (MQ=255)

                                                                   aGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGAGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTa                                                                       <  1:691869/140‑1 (MQ=255)

                                                                     ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                     <  2:1685772/140‑1 (MQ=255)

                                                                     ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                     <  1:1010763/140‑1 (MQ=255)

                                                                     ccGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                     <  1:4598879/140‑1 (MQ=255)

                                                                              tCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACg                                                            >  1:4984208/1‑140 (MQ=255)

                                                                                      gACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCcgcg                                                    >  1:4423557/1‑140 (MQ=255)

                                                                                       aCCATTTTTTGGGCGCGCGCAGGGTTTTGACGGAAGAGACGATGTTGTAGGATG‑G‑ATAAGGCGTTTACTCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTGTCATGCCTACTAGCCCTCGa                                                   >  2:609949/1‑140 (MQ=21)

                                                                                                tGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCg                                          >  2:1885567/1‑140 (MQ=255)

                                                                                                      cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                                    >  1:1231242/1‑140 (MQ=255)

                                                                                                      cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                                    <  1:1675919/140‑1 (MQ=255)

                                                                                                      cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACACTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGc                                    >  2:728880/1‑140 (MQ=255)

                                                                                                      cgcgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTGTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATAGTTGCGAGCCGc                                    >  2:545366/1‑140 (MQ=25)

                                                                                                        cgcAGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCtt                                  >  1:3276999/1‑140 (MQ=255)

                                                                                                           aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                               <  1:3524719/140‑1 (MQ=255)

                                                                                                           aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                               <  2:3777390/140‑1 (MQ=255)

                                                                                                           aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                               <  1:3179701/140‑1 (MQ=255)

                                                                                                           aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                               <  2:1125259/140‑1 (MQ=255)

                                                                                                           aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                               <  1:3051367/140‑1 (MQ=255)

                                                                                                           aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                               <  1:2593284/140‑1 (MQ=255)

                                                                                                           aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                               <  1:2578735/140‑1 (MQ=255)

                                                                                                           aGGATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTcc                               <  1:4833322/140‑1 (MQ=255)

                                                                                                            ggATTTTGACGGAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTccc                              <  1:1827032/140‑1 (MQ=255)

                                                                                                                 tgcaCGAAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTCAGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGatat                         <  2:803078/137‑1 (MQ=21)

                                                                                                                      ggAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaac                    <  1:3091320/140‑1 (MQ=255)

                                                                                                                       gAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca                   >  1:2349904/1‑140 (MQ=255)

                                                                                                                       gAAGAGACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaaca                   >  2:3389783/1‑140 (MQ=255)

                                                                                                                       gAAGAGACGATTTTGTAGGA‑C‑GTATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGCTATAAacca                   >  1:1477542/1‑140 (MQ=25)

                                                                                                                         agagACGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAaacaac                 >  1:5022786/1‑140 (MQ=255)

                                                                                                                          gagaCGATTTTGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAAcc                <  2:1165181/140‑1 (MQ=255)

                                                                                                                                  tttGTAGGA‑CGG‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGcc        >  2:2174062/1‑140 (MQ=255)

TGTATTTGGGCGATGGGCAATTTATCGAGTCGCCACGTACCGGCGAAACCATTCGGATAAGCCGATTAGCCGAACCTTTCTGGCAGGACCATTTTTTGGGCGCGCGCAGGATTTTGACGGAAGAGACGATTTTGTAAGA‑C‑G‑ATAAGGCGTTTACGCCGCATCCGGCAGTTGTACGCAGGTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACGAGCCCGCGAATATTTGCGAGCCGCTTTCCCGATATAAAACAACCTCATTGCCAACCTT  >  NZ_CP025268/247155‑247429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`