BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,622,099	T→C	234 (TAA→TAG)	hda ←	DnaA inactivator Hda

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,622,099	0	T	C	99.4%	1024.9 / NA	315	234 (TAA→TAG)	hda	DnaA inactivator Hda
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (173/139); total (175/140)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

ATGTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCCA  >  NZ_CP025268/2621963‑2622235

aTGTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTAc                                                                                                                                       <  1:2545293/140‑1 (MQ=255)

aTGTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTAc                                                                                                                                       <  1:3174885/140‑1 (MQ=255)

aTGTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTAc                                                                                                                                       <  1:3907758/140‑1 (MQ=255)

aTGTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTAc                                                                                                                                       >  1:502110/1‑140 (MQ=255)

   tgCGCCAGCGCTAGTTGAAGCACATGGCCGGATGCGACGCTTGCCACGTGTTATCGGGCCTACGAGGACTATGTATTAACTTCGCGTAGGTCGGATAAGGCGTTCGCGCGGCATCCGAGAAGAAACACCGTATCTACAAc                                                                                                                                    >  2:1749484/1‑140 (MQ=255)

    gCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACt                                                                                                                                   >  1:3536838/1‑140 (MQ=255)

    gCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACt                                                                                                                                   >  1:1889231/1‑140 (MQ=255)

     cTCCATCGCTAGTTGAAGCACATTGCGGGATGCGACGCTTTCCACGTTTTATCCGGCCTACGAATACTATGGATTTACTGCGCGTATTGCGGATATGGCGTTCGCGCTGCATCCTGTAATAAACACCTTATCTACAGCtt                                                                                                                                  >  2:1609191/1‑140 (MQ=255)

     cTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACtt                                                                                                                                  >  2:1077954/1‑140 (MQ=255)

        cATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAg                                                                                                                               >  1:2250210/1‑140 (MQ=255)

          tCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGaa                                                                                                                             >  1:2978834/1‑140 (MQ=255)

          tCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGaa                                                                                                                             >  2:4220529/1‑140 (MQ=255)

             cTAGTTGAAGCACATTGGCGGATGCGACGCTTGCCACGTCTTATCCGGCGTACGAATATTATGGTTTAAGTTCTGGTAGTTCGGATAAGGCGTTCGCGCGGTATCCGACTAGAAACACCTTATTTACAATTACAGAAttt                                                                                                                          >  2:1424070/1‑140 (MQ=255)

                gTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGCATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACACTCAACCCCTCATCTACAACTTCACAAgtcctt                                                                                                                       >  2:4959618/1‑140 (MQ=38)

                  tGAAGCACATTGCCGGATGCTACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTAGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAAtttctttc                                                                                                                     >  2:5036294/1‑140 (MQ=255)

                     aGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTcaca                                                                                                                  >  1:1014101/1‑140 (MQ=255)

                      gCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACaa                                                                                                                 <  2:3759915/140‑1 (MQ=255)

                          aTTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACgg                                                                                                             >  1:1052686/1‑140 (MQ=255)

                          aTTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACgg                                                                                                             >  2:750191/1‑140 (MQ=255)

                          aTTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACgg                                                                                                             >  1:1957737/1‑140 (MQ=255)

                              ccGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATg                                                                                                         <  1:1660175/140‑1 (MQ=255)

                               cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:750191/140‑1 (MQ=255)

                               cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  2:590408/140‑1 (MQ=255)

                               cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  1:2500033/140‑1 (MQ=255)

                               cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  2:4565074/140‑1 (MQ=255)

                               cGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCAGCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  2:3059029/140‑1 (MQ=255)

                               cGGATGCGACGCTTGCCACGTCTTATCCGGACTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATgg                                                                                                        <  2:4632963/140‑1 (MQ=255)

                                ggATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGt                                                                                                       <  2:2884854/140‑1 (MQ=255)

                                 gATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTc                                                                                                      >  1:478174/1‑140 (MQ=255)

                                 gATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTc                                                                                                      >  1:3258467/1‑140 (MQ=255)

                                        cGCTTGCCACGTCTTATCCGGCCTACGAATACTACGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAGTAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTAc                                                                                               >  2:3426436/1‑140 (MQ=255)

                                           ttGCCACGCCTTATCCGGCCTACGAATACTATCGATTAACTTCGCGTAGTTCGTATAAGGCGTTCGCGCCGCATCCGACCATAAACACCTTATCTCCTCCTTCAGAATTTCTTTCACAACCGGAATGGTCAGCTTACGtt                                                                                            <  2:445881/140‑1 (MQ=255)

                                            tGCCACGTCTTATCCGTCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTg                                                                                           <  1:2821439/140‑1 (MQ=255)

                                            tGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTg                                                                                           >  1:2064835/1‑140 (MQ=255)

                                            tGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTg                                                                                           >  1:87637/1‑140 (MQ=255)

                                              ccACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTgcg                                                                                         <  2:1486340/140‑1 (MQ=255)

                                                     ttATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAAt                                                                                  <  1:1716034/140‑1 (MQ=255)

                                                       aTCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAAtgg                                                                                >  1:4617937/1‑138 (MQ=255)

                                                       aTCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCg                                                                                <  2:2259886/140‑1 (MQ=255)

                                                          cGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACg                                                                             <  2:955799/140‑1 (MQ=255)

                                                            gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  1:3653030/140‑1 (MQ=255)

                                                            gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           <  1:2920696/140‑1 (MQ=255)

                                                            gCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCa                                                                           >  1:2320290/1‑140 (MQ=255)

                                                            gCCTACGAATACTATGGATAAACGTAGCGTAGTCAGGATAAAGCGTTACCGACGCATCCGACAATAAACACCTCATCCACACCTTCAGAATTACTTTCACAAACGCAATCGTCAGCTTACGCTGCGCGGTAATCGACGCa                                                                           <  2:2687692/140‑1 (MQ=255)

                                                             ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  2:832208/1‑140 (MQ=255)

                                                             ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  1:3709504/1‑140 (MQ=255)

                                                             ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  1:1245380/1‑140 (MQ=255)

                                                             ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  2:928043/1‑140 (MQ=255)

                                                             ccTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTATTTCACAAACTGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCAc                                                                          >  2:1583118/1‑140 (MQ=255)

                                                                     tACTCTGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAACCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAAc                                                                  <  2:4689923/140‑1 (MQ=255)

                                                                       cTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTg                                                                >  1:1288330/1‑140 (MQ=255)

                                                                       cTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTACACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTg                                                                >  1:4558067/1‑140 (MQ=255)

                                                                         aTGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGAt                                                              >  2:2030883/1‑140 (MQ=255)

                                                                          tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATc                                                             >  2:2592294/1‑140 (MQ=255)

                                                                          tGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATc                                                             >  2:2215337/1‑140 (MQ=255)

                                                                           ggATTAAGTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATcc                                                            >  2:2819016/1‑140 (MQ=255)

                                                                           ggATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATcc                                                            >  1:3770743/1‑140 (MQ=255)

                                                                           ggATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATcc                                                            >  1:593977/1‑140 (MQ=255)

                                                                           ggATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATcc                                                            >  1:4121435/1‑140 (MQ=255)

                                                                           ggATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATcc                                                            >  1:185294/1‑140 (MQ=255)

                                                                            gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCGGACAATACACACCTTATCTACAACTTCAGAATTTCCTTCACCAACGGAATGCTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:802119/140‑1 (MQ=255)

                                                                            gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:3511764/140‑1 (MQ=255)

                                                                            gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:3750517/140‑1 (MQ=255)

                                                                            gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:3128395/140‑1 (MQ=255)

                                                                            gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:3096260/140‑1 (MQ=255)

                                                                            gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:1204595/140‑1 (MQ=255)

                                                                            gATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCa                                                           <  2:1829383/140‑1 (MQ=255)

                                                                              ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  2:1156939/140‑1 (MQ=255)

                                                                              ttAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAAc                                                         <  1:1413119/140‑1 (MQ=255)

                                                                                aaCTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGt                                                       <  1:3605628/140‑1 (MQ=255)

                                                                                aaCTTCGCGTAGTTCGGAGAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGt                                                       <  2:546439/140‑1 (MQ=255)

                                                                                aaCTCCGCGTAGTTCGCATAATGCGTTCGCGCCGCATCCGCCAATGACCACCATATCTACAACTTCAGAATCTCTTTCACAAACGGAATCGTCAGCTTACGTCGCGCGGTAACCGACGCCCGATCCAACTGATCCAACGt                                                       <  2:2546551/140‑1 (MQ=255)

                                                                                      gcgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATaaa                                                 >  2:3894354/1‑140 (MQ=255)

                                                                                       cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAAt                                                >  2:2653970/1‑140 (MQ=255)

                                                                                       cgTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAAt                                                >  2:1517224/1‑140 (MQ=255)

                                                                                            ttCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGt                                           >  1:2732381/1‑140 (MQ=255)

                                                                                            ttCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGt                                           >  2:3663382/1‑140 (MQ=255)

                                                                                            ttCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGt                                           <  2:3581513/140‑1 (MQ=255)

                                                                                               ggATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTgcg                                        <  2:3241941/140‑1 (MQ=255)

                                                                                               ggATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTgcg                                        <  1:1479975/140‑1 (MQ=255)

                                                                                                      gCGTTCGCGCCGCATCCGACAATAAACACCTTATCTGCAACTTCAGAATGTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCCTAAATAGCGTGCGCATGtct                                 >  2:293550/1‑140 (MQ=255)

                                                                                                      gCGTTCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGGCGCACTATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTtct                                 >  2:3864357/1‑140 (MQ=255)

                                                                                                      gCGTTCGCGCCGCATACGACAATAAACAGCTTATCTACAACTTCAGAATTTCTATAACAAAGGGAGTGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTtcc                                 >  2:2103423/1‑139 (MQ=255)

                                                                                                         ttCGCGCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTg                              >  2:3924618/1‑140 (MQ=255)

                                                                                                           cgcgCCGCATCCGACAATAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCCTGCGCATTTCTCTGTc                            >  2:130030/1‑140 (MQ=255)

                                                                                                                   aTCCGACAATAAACCTCTTATCTACAACTTCAGAATTTCTTTAACACACGGAATGCTCAGCTTACGTCGCGCGGTAATCGACGCACGATCCAACTGACCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCt                    <  2:2677098/140‑1 (MQ=255)

                                                                                                                   aTCCGACAATAAACACCTGAGCTACAACCTAAAAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAAGCGACGAACGATCCAACGATTCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCt                    <  2:4760732/140‑1 (MQ=255)

                                                                                                                            tAAACACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGa           >  1:841441/1‑140 (MQ=255)

                                                                                                                              accacCTTATCTACAACTACATCATTTCTTTCACCAACGGATTGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa         <  2:1901635/138‑1 (MQ=255)

                                                                                                                              aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa         <  2:675368/140‑1 (MQ=255)

                                                                                                                              aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa         <  1:4211118/140‑1 (MQ=255)

                                                                                                                              aaCACCTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGaaa         <  1:2895976/140‑1 (MQ=255)

                                                                                                                                 acCTTATCTCCAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGc      <  1:5108463/140‑1 (MQ=255)

                                                                                                                                   cTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGccc    >  1:4174669/1‑140 (MQ=255)

                                                                                                                                   cTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGccc    >  1:1130053/1‑140 (MQ=255)

                                                                                                                                   cTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGccc    <  1:998897/140‑1 (MQ=255)

                                                                                                                                   cTTATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGGCAGCTGACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGccc    >  1:2174708/1‑140 (MQ=255)

                                                                                                                                     tATCTACAAGCTCAGAATTTCTTTCAAAAACGGCATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCAACCTGATCCAACGTCATAAATAGCGTGCGCATTTCTGTGTCGAGCCGCTTCAGCAAGAACCGCCCca  <  2:1225017/140‑1 (MQ=255)

                                                                                                                                     tATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGCTCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  <  2:4324507/140‑1 (MQ=255)

                                                                                                                                     tATCTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCca  <  1:1783938/140‑1 (MQ=255)

ATGTGCTCCATCGCTAGTTGAAGCACATTGCCGGATGCGACGCTTGCCACGTCTTATCCGGCCTACGAATACTATGGATTAACTTCGCGTAGTTCGGATAAGGCGTTCGCGCCGCATCCGACAATAAACACCTTATTTACAACTTCAGAATTTCTTTCACAAACGGAATGGTCAGCTTACGTTGCGCGGTAATCGACGCACGATCCAACTGATCCAACGTCATAAATAGCGTGCGCATTTCTCTGTCGAGCCGCTTCAGCAAGAAACGCCCCA  >  NZ_CP025268/2621963‑2622235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`