BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,649,037	T→C	771 (TAA→TAG)	pbpC ←	peptidoglycan glycosyltransferase PbpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,649,037	0	T	C	99.4%	548.8 / NA	170	771 (TAA→TAG)	pbpC	peptidoglycan glycosyltransferase PbpC
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (104/64); minor base A (1/0); total (105/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

TAAATTGTTCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCAC  >  NZ_CP025268/2648900‑2649172

tAAATTGTTCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGacta                                                                                                                                       >  2:2300216/1‑140 (MQ=255)

     tGTTCTGTTGTTGTCTGTACCAGCGCACGAGATGGCGCGGATTATAATGAGCAACAGGCCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCa                                                                                                                                  <  2:4606825/140‑1 (MQ=255)

      gTTCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCAt                                                                                                                                 <  1:3571504/140‑1 (MQ=255)

        tCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGa                                                                                                                               >  2:3536800/1‑140 (MQ=255)

        tCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGa                                                                                                                               >  1:1545175/1‑140 (MQ=255)

            ttgttgtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACaaa                                                                                                                           >  2:1127132/1‑140 (MQ=255)

               ttgtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAAttt                                                                                                                        >  1:3951862/1‑140 (MQ=255)

                 gtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCa                                                                                                                      >  2:1705065/1‑140 (MQ=255)

                 gtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCa                                                                                                                      >  1:1909005/1‑140 (MQ=255)

                 gtCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACAATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTTCATGACAAATTTCa                                                                                                                      >  1:2809409/1‑140 (MQ=255)

                    tGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTg                                                                                                                   >  1:2357469/1‑140 (MQ=255)

                      tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGGCGTGGACAATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                 >  2:1268578/1‑140 (MQ=255)

                      tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCCGGGACAAAACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                 >  1:1227315/1‑140 (MQ=255)

                      tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                 >  1:4422167/1‑140 (MQ=255)

                      tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                 >  1:1385738/1‑140 (MQ=255)

                      tACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTc                                                                                                                 >  1:1199809/1‑140 (MQ=255)

                         cAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTcgcg                                                                                                              >  1:2609392/1‑140 (MQ=255)

                           gCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAt                                                                                                            <  1:4469315/140‑1 (MQ=255)

                           gCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAt                                                                                                            <  2:4881339/140‑1 (MQ=255)

                           gCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAt                                                                                                            <  2:1385738/140‑1 (MQ=255)

                            cGTACGAGATGGCGCGGATTATAATGAGCAATAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                           >  2:1469933/1‑140 (MQ=255)

                            cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTCTTGATGAAGGGAACATTTTTTTAAAATAAAGTGAGGTTTAGCAACAATCAGGGACAAAACAGACTATTGCATCACAAATTTCACTGTCGCGAtt                                                                                                           >  2:2450444/1‑140 (MQ=255)

                            cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGACGGTAACATTTTTTTAAAATAAAATGAGCTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                           >  2:3091730/1‑140 (MQ=255)

                            cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                           >  2:2960579/1‑140 (MQ=255)

                            cGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGAtt                                                                                                           >  2:4725896/1‑140 (MQ=255)

                              tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTg                                                                                                         >  2:4431651/1‑140 (MQ=255)

                              tACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTg                                                                                                         >  1:1589510/1‑140 (MQ=255)

                                cGAGATGGCGCGGATTATCATGAGCAACAGGGCCGTTGACTATTGATGAAGGTCACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       >  2:744336/1‑140 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCACCAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTCTCGCGATTTGcc                                                                                                       >  1:1070902/1‑140 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       >  2:208174/1‑140 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       <  1:1108739/140‑1 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       <  2:4142086/140‑1 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       >  2:2879303/1‑140 (MQ=255)

                                cGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       >  1:3969756/1‑140 (MQ=255)

                                 gagaTGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGccc                                                                                                      <  2:2352901/140‑1 (MQ=255)

                                 aagaTGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAGATAAAATGAGTTTTAGCAACAATCAGGGACGAACCAGACTATTGCATGCCAAATTTCACTGTCGCGATTTGccc                                                                                                      <  2:4669575/139‑1 (MQ=255)

                                     tGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACg                                                                                                  <  2:43616/140‑1 (MQ=255)

                                     tGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACg                                                                                                  >  1:794400/1‑140 (MQ=255)

                                      ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       <  2:202769/134‑1 (MQ=255)

                                      ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGcc                                                                                                       >  1:202769/1‑134 (MQ=255)

                                      ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                 <  1:3037197/140‑1 (MQ=255)

                                      ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                 <  1:3771249/140‑1 (MQ=255)

                                      ggCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGt                                                                                                 <  1:2235714/140‑1 (MQ=255)

                                        cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGTGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTca                                                                                               >  2:4538175/1‑140 (MQ=255)

                                        cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTca                                                                                               >  2:5063506/1‑140 (MQ=255)

                                        cgcgGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAAGCAGGGACAAAACAGACGATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTca                                                                                               >  2:463754/1‑140 (MQ=255)

                                                      aGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCGCACTTCATCCATAACCAGCaa                                                                                 >  2:4337113/1‑140 (MQ=255)

                                                      aGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa                                                                                 >  1:3508347/1‑140 (MQ=255)

                                                      aGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCaa                                                                                 >  2:1575096/1‑140 (MQ=255)

                                                       gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATCAAATGAGTTTTAGCAACAATCAGTGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCGCGTCAGCCATCACCAGCAAt                                                                                >  2:3986865/1‑140 (MQ=255)

                                                       gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                >  2:3307284/1‑140 (MQ=255)

                                                       gCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAAt                                                                                >  1:4203655/1‑140 (MQ=255)

                                                       gCAACAGGGCCGTTGACTATCGATGAAGGTAACATTTTTTTAAAATAAACTTAGTTTTAGCAACAATCAGGGACAAAACAGCCTATTGCATGACAAATTTCACTGTCGCGCTTCGCCCCACGTCATCCATCACCACCaac                                                                                >  2:1210395/1‑139 (MQ=255)

                                                           cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAACATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGa                                                                             <  1:302871/139‑1 (MQ=255)

                                                           cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACTAATTTCACTGTCGCGATTTGCCCCACTTCATCCATCACCAGCAATGGAt                                                                            >  1:4376246/1‑140 (MQ=255)

                                                           cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGa                                                                             >  2:302871/1‑139 (MQ=255)

                                                           cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGAt                                                                            >  1:231199/1‑140 (MQ=255)

                                                           cAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGAt                                                                            >  1:3384604/1‑140 (MQ=255)

                                                           cAGGGCCGTGGACTATTGATGAAGGAAACCTTTTTTCAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACAATTGCACGACAAATATCACTGTCGCGATTTGCCCCACGTCACCCATCACCAGCAATCGag                                                                            >  2:1042217/1‑139 (MQ=255)

                                                             gggCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATaa                                                                          <  2:1817614/140‑1 (MQ=255)

                                                              ggCCGTTGACTATTGATAAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACACAACAGACTCTTGCATGCCAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAAt                                                                         <  1:214465/140‑1 (MQ=255)

                                                                ccGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCa                                                                       >  1:733770/1‑140 (MQ=255)

                                                                   ttGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCt                                                                    >  2:1758019/1‑140 (MQ=255)

                                                                   ttGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCt                                                                    >  2:4156702/1‑140 (MQ=255)

                                                                       cTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTAt                                                                <  2:2382168/140‑1 (MQ=255)

                                                                        tATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCAc                                                                                       <  2:2772893/116‑1 (MQ=255)

                                                                            gatgaAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTc                                                           <  1:8430/140‑1 (MQ=255)

                                                                                 aGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATg                                                      <  1:593103/140‑1 (MQ=255)

                                                                                 aGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATg                                                      <  1:1041098/140‑1 (MQ=255)

                                                                                  ggTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGGCAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTGTCCGTCAAATGc                                                     >  1:4352926/1‑140 (MQ=255)

                                                                                   ggaataTTTTTTTCAAATAAGATGAGTTTTAGCAACAATCAGGGACAGAACAGAGTAGTGCATGCCAAATTTCACTGTCGCGACTTGCCCCACGTCATCCATCACCAGCAATCGATAATCACCTTTTTCCGTCAAATGCa                                                    <  2:3359284/135‑1 (MQ=255)

                                                                                   gTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCa                                                    <  2:262763/140‑1 (MQ=255)

                                                                                   gTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCa                                                    <  2:2099026/140‑1 (MQ=255)

                                                                                   gTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCa                                                    <  1:4052773/140‑1 (MQ=255)

                                                                                    tAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAg                                                   >  1:1764359/1‑140 (MQ=255)

                                                                                    tAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAg                                                   >  1:1667695/1‑140 (MQ=255)

                                                                                            ttttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGt                                           >  2:1594898/1‑140 (MQ=255)

                                                                                             tttAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGtt                                          >  1:53589/1‑140 (MQ=255)

                                                                                                  aataaaatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCTcc                                     >  2:1949165/1‑140 (MQ=255)

                                                                                                      aaatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACCAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCcgcg                                 <  2:758760/140‑1 (MQ=255)

                                                                                                       aatGAGTTTTAGCAACAATCAGGGACAAAACGGACTATTGCATGACAAATTTCACTGTCGCGATTGGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGt                                <  2:71984/140‑1 (MQ=255)

                                                                                                       aatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGt                                <  1:486590/140‑1 (MQ=255)

                                                                                                       aatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGt                                <  1:1575096/140‑1 (MQ=255)

                                                                                                       aatGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGt                                <  2:2761794/140‑1 (MQ=255)

                                                                                                        atGAGTTTTAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGtt                               <  1:1668902/140‑1 (MQ=255)

                                                                                                             ttttAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGtt                          >  1:2003907/1‑140 (MQ=255)

                                                                                                             ttttAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGtt                          <  1:1525693/140‑1 (MQ=255)

                                                                                                               ttAGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTCTCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTaa                        <  2:2100734/140‑1 (MQ=255)

                                                                                                                 aGCAACAATCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACg                      >  1:1704956/1‑140 (MQ=255)

                                                                                                                     acaaTCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTc                  >  2:2653516/1‑140 (MQ=255)

                                                                                                                       aaTCAGGGACAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGGTCAGTTAACGGTTCGc                >  2:2421272/1‑140 (MQ=255)

                                                                                                                               aCAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCACCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGa        <  2:1209942/140‑1 (MQ=255)

                                                                                                                                cAAAACAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCACATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGaa       >  2:4859838/1‑140 (MQ=255)

                                                                                                                                 aaaaCAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTTCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGaaa      >  2:3991509/1‑140 (MQ=255)

                                                                                                                                 aaaaCAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGaaa      >  2:1923638/1‑140 (MQ=255)

                                                                                                                                   aaCAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAacg    >  1:4503062/1‑139 (MQ=255)

                                                                                                                                   aaCAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAacc    >  1:1707212/1‑140 (MQ=255)

                                                                                                                                     cAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAaccac  <  2:3442647/140‑1 (MQ=255)

                                                                                                                                     cAGACTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAaccac  <  1:4494820/140‑1 (MQ=255)

                                                                                                                                     cAGACTATTGCATGACAAATTTCACTGTCGCGACTTGCCCCACGTCATCCATCCCCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCACTTAACGGGTCGCCATTCAGAAaccac  <  2:214379/140‑1 (MQ=255)

TAAATTGTTCTGTTGTTGTCTGTACCAGCGTACGAGATGGCGCGGATTATAATGAGCAACAGGGCCGTTGACTATTGATGAAGGTAACATTTTTTTAAAATAAAATGAGTTTTAGCAACAATCAGGGACAAAACAGATTATTGCATGACAAATTTCACTGTCGCGATTTGCCCCACGTCATCCATCACCAGCAATTGATAATCACCTTTATCCGTCAAATGCAGGGTCACGTTGCGCCCGCGTTCAGTTAACGGTTCGCCATTCAGAAACCAC  >  NZ_CP025268/2648900‑2649172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`