BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,659,791	C→T	A198T (GCT→ACT)	pepB ←	aminopeptidase PepB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,659,791	0	C	T	99.1%	1239.2 / NA	341	A198T (GCT→ACT)	pepB	aminopeptidase PepB
Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (156/180); total (158/183)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 341 total aligned reads displayed.

CAGCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCTCACGC  >  NZ_CP025268/2659654‑2659927

cAGCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGa                                                                                                                                        >  1:284772/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  1:102977/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  1:1189850/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  2:3831725/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  1:886269/1‑140 (MQ=255)

  gCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATa                                                                                                                                      >  1:81115/1‑140 (MQ=255)

   cTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATAc                                                                                                                                     <  1:3700234/140‑1 (MQ=255)

        cgcgCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTta                                                                                                                                <  2:4457796/140‑1 (MQ=255)

        cgcgCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTta                                                                                                                                <  2:1647558/140‑1 (MQ=255)

        cgcgCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTta                                                                                                                                >  1:4618912/1‑140 (MQ=255)

        cgcgCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTta                                                                                                                                <  1:1938948/140‑1 (MQ=255)

        cgcgCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGACCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTta                                                                                                                                <  1:1748313/140‑1 (MQ=255)

             ttGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTtacctac                                                                                                                           >  2:843317/1‑140 (MQ=255)

             ttGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTtacctac                                                                                                                           >  1:4297837/1‑140 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  2:1257638/140‑1 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  1:1402071/140‑1 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  2:3685467/140‑1 (MQ=255)

                 tCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  1:3178394/140‑1 (MQ=255)

                 ccAGTCCGCGCGTCATGGCAAAGGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAgg                                                                                                                       <  2:1431824/139‑1 (MQ=255)

                  cAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGc                                                                                                                      <  1:4025448/140‑1 (MQ=255)

                    gTCCGCGCGTAATGGCAAATGCCAGCGCCCCTGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCAc                                                                                                                    <  2:4189215/140‑1 (MQ=255)

                     tCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCAcg                                                                                                                   >  1:4468670/1‑140 (MQ=255)

                         cgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTa                                                                                                               >  1:4923198/1‑140 (MQ=255)

                         cgcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTa                                                                                                               >  2:3979892/1‑140 (MQ=255)

                          gcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTac                                                                                                              >  1:2925535/1‑140 (MQ=255)

                          gcgTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTac                                                                                                              >  1:1434234/1‑140 (MQ=255)

                          gcgTAAGGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTac                                                                                                              >  2:4517919/1‑140 (MQ=255)

                               aTGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTgg                                                                                                         >  1:3117338/1‑140 (MQ=255)

                                         ccAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTa                                                                                               <  1:4203165/140‑1 (MQ=255)

                                           aGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGGCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATc                                                                                             >  2:2969564/1‑140 (MQ=255)

                                                  cGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAgat                                                                                      >  1:2446261/1‑138 (MQ=255)

                                                  cGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGtt                                                                                      >  2:2417709/1‑140 (MQ=255)

                                                   ggaaaCAGTAGCAGCGCCGTCCATGTCCGACTTCAACGAGACCATACACGCAGTCCATTTGAAGCTGTAGCCGCCGGAGTAAAAAGTGATACCTTTACCTACCAGGCACGAGTACACTGGCGCTTCATTATCGCCAGTTg                                                                                     <  2:45992/137‑1 (MQ=255)

                                                   ggTTACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCCGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAACGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTg                                                                                     <  2:2503722/140‑1 (MQ=255)

                                                   ggTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTg                                                                                     <  2:1312360/140‑1 (MQ=255)

                                                       aCCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGtt                                                                                 >  1:3933955/1‑140 (MQ=255)

                                                            tGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTGCTTTATCGCCAGGTGGGTTGTAAt                                                                            >  1:1816437/1‑140 (MQ=255)

                                                             gccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATc                                                                           <  1:2290838/140‑1 (MQ=255)

                                                             gccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATc                                                                           <  1:1173607/140‑1 (MQ=255)

                                                              ccgcgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGGTGTAATcc                                                                          >  1:1105329/1‑140 (MQ=255)

                                                                 cgccgcCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAgc                                                                       >  1:2328960/1‑140 (MQ=255)

                                                                    cgccCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGcc                                                                    <  2:58842/140‑1 (MQ=255)

                                                                      cccATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAg                                                                  >  1:4412069/1‑140 (MQ=255)

                                                                         ctttCCGACCTCATCGAGTCCATAAACGCAGTCTGTTTGCTGCCGGAGCCGCCGGATTCAAAAGCGATACCTTCACCCCCCAGGCATGCCTACACTGGCGCTTCTCAATCTCCAGTGGGGTTGTGATCCAGCGCCAGCaa                                                               <  2:3497308/137‑1 (MQ=255)

                                                                         aTGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACGTTTACCTACCGGGCACGCGTACACTGGTGCTTCGCTATGGCCAGTTGGGTTGTAAGCCAGCGCCAGCaa                                                               <  2:4098964/140‑1 (MQ=255)

                                                                         aTGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCaa                                                               <  2:3455106/140‑1 (MQ=255)

                                                                             ccGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATAcc                                                           <  2:3226562/140‑1 (MQ=255)

                                                                                acatCATAGAGACCATAAACGCAGGCTGTTTGATGCTGTAGCCGCCGCAGGCAAAAGTTGTCCCTTTACCTACCCATCACGCGTACACTGGCGCTCCCTAATGGCCAGTCGGGTTGTAATCAAGCGCACGCAATCGCGga                                                        >  2:4780025/4‑140 (MQ=255)

                                                                                aCTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGga                                                        >  1:622446/1‑140 (MQ=255)

                                                                                aCTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGga                                                        >  1:3834334/1‑140 (MQ=255)

                                                                                  ttCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                                      <  1:558923/140‑1 (MQ=255)

                                                                                  ttCATCGAGTCCATAAACGCAGTCTCTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGAGACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGgaga                                                      <  2:3369971/140‑1 (MQ=255)

                                                                                    cATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTtaccta                                                                                                                            <  2:3901101/68‑1 (MQ=255)

                                                                                    cATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTtaccta                                                                                                                            >  1:3901101/1‑68 (MQ=255)

                                                                                         aGTCCATAAACGCAGTCTGTTTGATGCTGTATCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCt                                               >  2:282909/1‑140 (MQ=255)

                                                                                            ccATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGaa                                            <  2:714426/140‑1 (MQ=255)

                                                                                            ccATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGaa                                            >  2:1459144/1‑140 (MQ=255)

                                                                                            ccATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGaa                                            <  1:4712131/140‑1 (MQ=255)

                                                                                            ccATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGaa                                            <  1:3613734/140‑1 (MQ=255)

                                                                                            ccATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGaa                                            <  1:4086537/140‑1 (MQ=255)

                                                                                              aTAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAAcc                                          <  1:4802004/140‑1 (MQ=255)

                                                                                              aTAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAAcc                                          <  1:1225562/140‑1 (MQ=255)

                                                                                                   cGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTc                                     <  1:525762/140‑1 (MQ=255)

                                                                                                     cAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGCCACTCGTACACTGGCGCTTCTTTAGCGCCCGTTGGCTTGTAATCCAGCTCCAGCAATACCGGGGGACCTTCTGGACCGCGTCCg                                   >  2:357030/1‑140 (MQ=255)

                                                                                                      tgTCTGTTTGATGCTGTAGCCGCCTCAGTCAAAAGTGATTCCTTTACCTACCAGGAACGCGTACACTGGCGCTTCTTTAACGCCAGATGGGTTGTAATCCTGCGCCCGCTATACCGGAGAACGTTCTGAACCGCGTCCGa                                  <  2:4955950/139‑1 (MQ=255)

                                                                                                      aGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGa                                  <  2:2147720/140‑1 (MQ=255)

                                                                                                       gTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGAc                                 >  1:4872419/1‑140 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                >  2:2592739/1‑140 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  1:1668905/140‑1 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                >  2:386382/1‑140 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  1:257575/140‑1 (MQ=255)

                                                                                                        tCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTCGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                <  2:1787540/140‑1 (MQ=255)

                                                                                                          tGTTTGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACt                                >  2:4674793/1‑138 (MQ=255)

                                                                                                             ttGATGCTGTAGCCGCCGGAGTCAAAAGTGCTACCTTTACCTACCAGGCACGCGTACACTGCCGCTTCTTTATCGCCAGTTGGGTTGTAATCCCGCGCCAGCAACACCGGAGAACGTTCTGCACCGCGTCCGACtgtttg                           <  2:3067246/140‑1 (MQ=255)

                                                                                                             ttGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                           <  1:2215386/140‑1 (MQ=255)

                                                                                                             ttGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                           >  2:2290082/1‑140 (MQ=255)

                                                                                                             ttGATGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACtgtgtg                           <  2:2332488/140‑1 (MQ=255)

                                                                                                                atcatGTAGCCGCGGGAGTCAAAAGTGATACCTTTAACTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCAAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  2:1012774/136‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  2:615458/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  1:2760233/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  1:1529026/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCAAGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        >  2:4015721/1‑140 (MQ=255)

                                                                                                                aTGCTGTAGCCGCCGGAGTCAAAAGCGAGACCTTTACCTACCAGGGACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATAACGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  2:4399248/140‑1 (MQ=255)

                                                                                                                aTGCTGTAGCCGACGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAg                        <  1:2150602/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATCCCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  2:284772/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  1:2648756/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  1:2352480/140‑1 (MQ=255)

                                                                                                                 tGCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       <  1:1601783/140‑1 (MQ=255)

                                                                                                                  gCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcc                      <  1:3491074/140‑1 (MQ=255)

                                                                                                                  gCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcc                      <  2:4283347/140‑1 (MQ=255)

                                                                                                                  gCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcc                      <  1:2115867/140‑1 (MQ=255)

                                                                                                                  gCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcc                      >  2:516200/1‑140 (MQ=255)

                                                                                                                  gCTGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcc                      <  1:4534237/140‑1 (MQ=255)

                                                                                                                    tGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcccc                    >  2:3334407/1‑140 (MQ=255)

                                                                                                                    tGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGcccc                    >  2:1126908/1‑140 (MQ=255)

                                                                                                                    tGTAGCCGCCGGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGc                       >  1:3397819/1‑137 (MQ=255)

                                                                                                                           gccgGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCCTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAAc             >  1:3691077/1‑140 (MQ=255)

                                                                                                                             cgGAGTCAAAAGTGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCGGCCCCATATAACCt           >  2:189071/1‑140 (MQ=255)

                                                                                                                                gcacAAAAGTGATACCCTTACCTGCCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTCATCCAGCGCCAGCAATGCCGGAGACCGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGc        <  2:5062371/137‑1 (MQ=255)

                                                                                                                                      aaGTGATACCTGTACCTCCCAGGCACGCCTACACTGGCGCTTCTTTCTCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAAAGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCTCACGc  <  2:2722176/140‑1 (MQ=255)

CAGCTTCACGCGCTTGTTCAGTCCGCGCGTAATGGCAAATGCCAGCGCCCCGGTAACCGTTGCCGCGCCGCCCATGTCCGACTTCATCGAGTCCATAAACGCAGTCTGTTTGATGCTGTAGCCGCCGGAGTCAAAAGCGATACCTTTACCTACCAGGCACGCGTACACTGGCGCTTCTTTATCGCCAGTTGGGTTGTAATCCAGCGCCAGCAATACCGGAGAACGTTCTGAACCGCGTCCGACTGTGTGCAGCCCCATATAACCTTGCTCACGC  >  NZ_CP025268/2659654‑2659927

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`