BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,746,986	A→G	N194D (AAT→GAT)	dgcN →	diguanylate cyclase DgcN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,746,986	0	A	G	99.6%	773.8 / NA	242	N194D (AAT→GAT)	dgcN	diguanylate cyclase DgcN
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (157/82); total (159/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 243 total aligned reads displayed.

TCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTA  >  NZ_CP025268/2746847‑2747120

  gcgACAGTTCAATCAGCCATTGTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGAt                                                                                                                                      <  2:3387450/140‑1 (MQ=255)

    gACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGt                                                                                                                                    <  2:1154912/140‑1 (MQ=255)

    gACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGt                                                                                                                                    <  2:3858356/140‑1 (MQ=255)

    gACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGt                                                                                                                                    <  1:2688779/140‑1 (MQ=255)

       aGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGt                                                                                                                                 <  1:2984546/140‑1 (MQ=255)

         ttCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTAc                                                                                                                               >  1:1085237/1‑140 (MQ=255)

            aaTCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACatg                                                                                                                            >  1:3511952/1‑140 (MQ=255)

              tCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCGCAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACatgat                                                                                                                          >  2:4077181/1‑140 (MQ=255)

              tCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACatgat                                                                                                                          >  1:1476223/1‑140 (MQ=255)

              tCAGCCATTTTATCTGGTTTTCGCTCGCCGCACTGACCGGTTGTATTCTGCTGTCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACatgat                                                                                                                          >  1:5004436/1‑140 (MQ=255)

               aacccATTTTATCTGGTTTTCGCCCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGAATCGCAATTACCCTCACCCGCCATTTGCCCACTGGCCTGGTAGCAGCGCTGAAAAATATCACCGATGTCGTACatgatg                                                                                                                         <  2:1435719/137‑1 (MQ=255)

                 gCCATTTTATCTGGTTTTGGCTCGACGTATTGACAGGTTGTATTCTGATGTAATCAGGGATCGCCATTACCCTCACCCGCCATTTGCAGAATGGCCTGGTAGAAGCGATCAAAAATATCACAAAGATCGTAAatgatggg                                                                                                                       >  2:223882/1‑138 (MQ=255)

                 gCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGAtgtg                                                                                                                       >  2:3266465/1‑140 (MQ=255)

                  ccATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGc                                                                                                                      >  1:3419633/1‑140 (MQ=255)

                     ttttATCTGGTTTTCGCTCGCCGTACTGACCGTTTGTAGTCTGCTGTCATCAGTCATCGCAATTACCCTCACCCGGCATTTGCACAATGGCGGGTTAGAAGCGCGGAAAAAGATCTGCGGTGTCGTACATGATGTGCttt                                                                                                                   >  2:3654786/1‑140 (MQ=255)

                               ttttCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCaa                                                                                                         >  1:919816/1‑140 (MQ=255)

                                tttCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAAt                                                                                                        >  2:393063/1‑140 (MQ=255)

                                      tcgcCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTcc                                                                                                  <  1:2795313/140‑1 (MQ=255)

                                        gcCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTTCAAACGCAATTTTTCCcg                                                                                                >  2:2204109/1‑140 (MQ=255)

                                         ccGTACGGACCGGTTGTATTCTGCGGGCATCAGGCATCGCAAGTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCcga                                                                                               <  2:5102020/140‑1 (MQ=255)

                                                  ccGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCg                                                                                      >  1:2543513/1‑140 (MQ=255)

                                                      ttGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGgaag                                                                                  >  1:4698941/1‑140 (MQ=255)

                                                      ttGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGgaag                                                                                  >  2:2448108/1‑140 (MQ=255)

                                                      ttGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGgaag                                                                                  >  1:709793/1‑140 (MQ=255)

                                                          aTTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACg                                                                              <  1:461888/140‑1 (MQ=255)

                                                               gctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCg                                                                         >  2:1079359/1‑140 (MQ=255)

                                                               gctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCg                                                                         <  1:3766469/140‑1 (MQ=255)

                                                                ctgGCATCAGGGATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGACATACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  1:4228131/1‑140 (MQ=255)

                                                                ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGCTGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGGAGAACGTATCGc                                                                        >  2:3703777/1‑140 (MQ=255)

                                                                ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  2:1958904/1‑140 (MQ=255)

                                                                ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  1:1428160/1‑140 (MQ=255)

                                                                ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  2:4787724/1‑140 (MQ=255)

                                                                ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  1:3673896/1‑140 (MQ=255)

                                                                ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTCCGTCCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGc                                                                        >  2:73119/1‑140 (MQ=255)

                                                                ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGGAGAAGCGCTGAAAAATATCACCGATGTCGTACATGGTGTGCGTTCCAACCGCCAGTATTCCCGCCGAGTTTGGGAAGAACGTATCga                                                                        >  2:2106309/1‑139 (MQ=255)

                                                                ctgGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCAGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGGTCCAACCGAAATTTTTCCCGACGAGTTGCGAAAGAACGTATCGc                                                                        >  2:2593761/1‑140 (MQ=255)

                                                                ctgGCATCAGGCATCGCAATTACCCTCACACGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCACTTTTTCCCGACGAGGTTCGGAAGAACGTATCGc                                                                        >  1:1233921/1‑140 (MQ=255)

                                                                   gCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGa                                                                     >  1:4535590/1‑140 (MQ=255)

                                                                   gCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCGGa                                                                     >  2:4553250/1‑140 (MQ=255)

                                                                        aGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTc                                                                >  2:2396039/1‑140 (MQ=255)

                                                                          gCATCGCAATTACCCTCACACGCGATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAAAATGACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCACGACGAGTTTCGGAAGCACGTAGCGCTGAATTTCAc                                                              >  2:1067510/1‑140 (MQ=255)

                                                                              cGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCt                                                          >  1:1295274/1‑140 (MQ=255)

                                                                               gCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCtt                                                         <  1:2748202/140‑1 (MQ=255)

                                                                               gCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCtt                                                         >  1:1673289/1‑140 (MQ=255)

                                                                               gCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCtt                                                         <  1:2219980/140‑1 (MQ=255)

                                                                               gCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTCCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCta                                                         >  1:4503754/1‑139 (MQ=255)

                                                                                     aCCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGctct                                                   >  2:4503393/1‑140 (MQ=255)

                                                                                      cccTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCtctc                                                  <  1:1107968/140‑1 (MQ=255)

                                                                                      cccTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCtctc                                                  <  2:2864453/140‑1 (MQ=255)

                                                                                      cccGCACCCGCCATCTTCCCTATGGCCTGGTAGATGCGCTGAAAAATATCACCTATGTCGTAGAAGGTGTGCGTCCCATCCGCAAGTGTTCCCGTCGAGTGTCTGAAGAATGTATCGCTGAGTTTCACCGCTTCGCtctc                                                  <  2:1704808/140‑1 (MQ=255)

                                                                                           aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACtt                                             >  2:396272/1‑140 (MQ=255)

                                                                                           aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACtt                                             >  1:3086901/1‑140 (MQ=255)

                                                                                           aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACtt                                             >  1:1236169/1‑140 (MQ=255)

                                                                                           aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACtt                                             >  1:490132/1‑140 (MQ=255)

                                                                                           aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACtt                                             >  1:569879/1‑140 (MQ=255)

                                                                                           aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACtt                                             >  1:4484132/1‑140 (MQ=255)

                                                                                           aCCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGGACATGATGTGCGTGCCAACCGCAATGTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCGTCGCTCTCGACtt                                             >  2:4292734/1‑140 (MQ=255)

                                                                                            cccGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTc                                            <  2:1231178/140‑1 (MQ=255)

                                                                                                ccATTTGCACAATGGCCTGGTAGAAGTGCTGAATAATATCACCGATGTAGTACATGATGTTCGTTCCAACCGCAAGATTTCCTGCTGAGTTTCGGAAGAGCGTAGCGCTGAGATAAACAGGTTCGCACTCGACTTCAACa                                        >  2:2618975/1‑140 (MQ=255)

                                                                                                ccATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACa                                        <  1:4997568/140‑1 (MQ=255)

                                                                                                ccATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACa                                        >  1:293384/1‑140 (MQ=255)

                                                                                                ccATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACa                                        >  2:194116/1‑140 (MQ=255)

                                                                                                ccATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTCCAACa                                        >  2:919208/1‑140 (MQ=255)

                                                                                                ccATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCAACGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACa                                        >  2:544085/1‑140 (MQ=255)

                                                                                                  aTTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGt                                      <  2:903123/140‑1 (MQ=255)

                                                                                                     tGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctg                                   >  1:4154722/1‑140 (MQ=255)

                                                                                                     tGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctg                                   <  2:4969790/140‑1 (MQ=255)

                                                                                                     tGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctg                                   >  2:2740286/1‑140 (MQ=255)

                                                                                                       cacaATGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTctgct                                 >  1:13511/1‑140 (MQ=255)

                                                                                                          aaTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              <  2:1785932/140‑1 (MQ=255)

                                                                                                          aaTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              <  1:139927/140‑1 (MQ=255)

                                                                                                          aaTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              <  1:2058865/140‑1 (MQ=255)

                                                                                                          aaTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGa                              <  1:5079524/140‑1 (MQ=255)

                                                                                                           aTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGAt                             >  1:3948809/1‑140 (MQ=255)

                                                                                                           aTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGAt                             >  1:4278806/1‑140 (MQ=255)

                                                                                                           aTGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGAt                             >  1:4327242/1‑140 (MQ=255)

                                                                                                            tGGCCTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATg                            <  1:894869/140‑1 (MQ=255)

                                                                                                               ccTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGGGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATaaaa                         >  2:4901587/1‑140 (MQ=255)

                                                                                                                cTGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAAt                        <  2:3034386/140‑1 (MQ=255)

                                                                                                                 tGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATg                       >  1:667648/1‑140 (MQ=255)

                                                                                                                 tGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATg                       >  1:1608964/1‑140 (MQ=255)

                                                                                                                 tGGTAGAAGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTGCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATg                       >  1:2204797/1‑140 (MQ=255)

                                                                                                                   gTAGAAGCGCTGCAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTATCGGAACAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGa                     <  2:2477997/140‑1 (MQ=255)

                                                                                                                   gTAGAAGCGCTGAAAAATATCACCGATGTCGTACATCATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGa                     <  1:5027547/140‑1 (MQ=255)

                                                                                                                        aGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAagagg                >  2:3787676/1‑139 (MQ=255)

                                                                                                                        aGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCGGCTGGATGAAATGGAAGAGt                >  1:1732440/1‑140 (MQ=255)

                                                                                                                        aGCGCTGAAAAATATCACCGATGTCGTACATGATGTGCGGTCCAACCGCAATTTTTCGCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCGGGATGAAATGGAAGAGt                >  1:463666/1‑140 (MQ=255)

                                                                                                                          cgcTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTgg              <  2:2841590/140‑1 (MQ=255)

                                                                                                                          cgcTGAAAAATATCACCGATGTCGTACATGATGGGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTgg              >  2:758738/1‑140 (MQ=255)

                                                                                                                          ccctcacaaaTATCACCGATGTCGTACATGATGTGCGTTCCATCCGCACTTTTTCCCGCCGAGTTTCGGAAGCACGTATCGCTGAGGTTCACCGCTTCGCTCTCGACTTCCACGGTCTGCTGGATGCACTGAAAGAGTgg              <  2:3051828/133‑1 (MQ=255)

                                                                                                                           gcTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGc             >  1:4776743/1‑140 (MQ=255)

                                                                                                                            cTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa            <  2:674344/140‑1 (MQ=255)

                                                                                                                            cTGAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCa            <  2:4361205/140‑1 (MQ=255)

                                                                                                                             tGAAAAATATCACCGATCTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACATATAGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGAAGAAATGGAAGAGTGACAg           >  2:3666180/1‑140 (MQ=255)

                                                                                                                              gAAAAATATCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGc          <  2:1648642/140‑1 (MQ=255)

                                                                                                                                    tatCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGtt    >  1:3268917/1‑140 (MQ=255)

                                                                                                                                      tCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  <  1:1764197/140‑1 (MQ=255)

                                                                                                                                      tCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  <  1:2942516/140‑1 (MQ=255)

                                                                                                                                      tCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  <  1:1045163/140‑1 (MQ=255)

                                                                                                                                      tCACCGATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTACAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTa  <  2:4652239/140‑1 (MQ=255)

TCGCGACAGTTCAATCAGCCATTTTATCTGGTTTTCGCTCGCCGTACTGACCGGTTGTATTCTGCTGGCATCAGGCATCGCAATTACCCTCACCCGCCATTTGCACAATGGCCTGGTAGAAGCGCTGAAAAATATCACCAATGTCGTACATGATGTGCGTTCCAACCGCAATTTTTCCCGACGAGTTTCGGAAGAACGTATCGCTGAGTTTCACCGCTTCGCTCTCGACTTCAACAGTCTGCTGGATGAAATGGAAGAGTGGCAGCTTCGTTTA  >  NZ_CP025268/2746847‑2747120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`