Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,081,156 C→T S3N (AGC→AAC)  cmtA ← PTS mannitol transporter subunit IICB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,081,1560CT99.6% 1037.8 / NA 280S3N (AGC→AAC) cmtAPTS mannitol transporter subunit IICB
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (89/189);  total (91/189)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

ATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAAT  >  NZ_CP025268/3081020‑3081292
                                                                                                                                        |                                                                                                                                        
aTGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGtttt                                                                                                                                       >  1:2299607/1‑140 (MQ=255)
 tGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCCGTAATAACACCCCAGGCAATAAACGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTc                                                                                                                                      <  1:2983315/140‑1 (MQ=255)
   ttCGTTAGGCAGCCAACCTGTGGAAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATTACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCa                                                                                                                                    <  2:1248148/140‑1 (MQ=255)
   ttCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCa                                                                                                                                    <  1:101741/140‑1 (MQ=255)
   ttCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCa                                                                                                                                    <  1:1141342/140‑1 (MQ=255)
   ttCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCa                                                                                                                                    <  1:5099768/140‑1 (MQ=255)
   ttCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCa                                                                                                                                    <  1:4413850/140‑1 (MQ=255)
   ttCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCa                                                                                                                                    <  2:2796086/140‑1 (MQ=255)
   ttCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCa                                                                                                                                    <  1:2579149/140‑1 (MQ=255)
   ttCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCa                                                                                                                                    <  1:2196688/140‑1 (MQ=255)
   ttCGGTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCa                                                                                                                                    <  2:828701/140‑1 (MQ=255)
   ctCGTTACGAACCCAACCGGTGCAAATAAATAACGTAGTAATCAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCCAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCa                                                                                                                                    <  2:5023085/139‑1 (MQ=255)
        tAGGCAGCCAACCGGTGGGAATAAATAACTCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCataata                                                                                                                               <  2:1486588/140‑1 (MQ=255)
        tAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCataata                                                                                                                               <  2:1498683/140‑1 (MQ=255)
        tAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCataata                                                                                                                               <  2:68394/140‑1 (MQ=255)
           gCAGCCAACCGGTGGGAATAAATACAGCAGTAATCAAACCCCAGGCAATAAACGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGCCCCTTTGCCCGAGCAGACTTGTTTTCCATAAtatta                                                                                                                            <  2:917405/140‑1 (MQ=255)
           gCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAAtatta                                                                                                                            <  2:1981491/140‑1 (MQ=255)
             agacAACCGGTGGGAATAAATAAAGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATc                                                                                                                          <  2:2348398/137‑1 (MQ=255)
             aGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCATTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATc                                                                                                                          <  2:4687192/140‑1 (MQ=255)
             aGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATc                                                                                                                          <  2:1145867/140‑1 (MQ=255)
             aGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATc                                                                                                                          <  1:4658504/140‑1 (MQ=255)
             aGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATc                                                                                                                          <  1:4127661/140‑1 (MQ=255)
             aGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATc                                                                                                                          <  1:194274/140‑1 (MQ=255)
             aGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATc                                                                                                                          <  1:2653125/140‑1 (MQ=255)
             aGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATc                                                                                                                          <  1:4084405/140‑1 (MQ=255)
             aGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATc                                                                                                                          <  2:3179032/140‑1 (MQ=255)
             aGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGAAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATc                                                                                                                          <  2:2114507/140‑1 (MQ=255)
             aGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGCCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATc                                                                                                                          <  2:4086128/140‑1 (MQ=255)
               ccAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCt                                                                                                                        >  1:29241/1‑140 (MQ=255)
               ccAACCGGTGGGAATAAATAACGCACTAATAAAACCCCAGGCAATAAAAGCACCAATAGTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCt                                                                                                                        <  2:3369209/140‑1 (MQ=255)
                 aaCCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGt                                                                                                                      <  1:3781734/140‑1 (MQ=255)
                  aCCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATCAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGCGCAGACTTGTTTTCCATAATATTATCCTGTa                                                                                                                     <  1:192909/140‑1 (MQ=255)
                  aCCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAACGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTa                                                                                                                     <  1:171680/140‑1 (MQ=255)
                  aCCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTa                                                                                                                     <  2:1734464/140‑1 (MQ=255)
                  aCCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTa                                                                                                                     <  1:127544/140‑1 (MQ=255)
                    cGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTaga                                                                                                                   <  1:2506765/140‑1 (MQ=255)
                     ggTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTagag                                                                                                                  <  1:2767919/140‑1 (MQ=255)
                       tGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGCCTTGTTTTCCATCATATTATCCTGTAgagga                                                                                                                <  2:1605771/140‑1 (MQ=255)
                       tGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAgagga                                                                                                                <  2:3924854/140‑1 (MQ=255)
                         ggAATAAATACCGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGgaga                                                                                                              <  2:3771915/140‑1 (MQ=255)
                              aaataaCGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTa                                                                                                         >  1:3679158/1‑140 (MQ=255)
                                 taaCGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAAtt                                                                                                      >  1:4546692/1‑140 (MQ=255)
                                    cGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATc                                                                                                   >  2:1672384/1‑140 (MQ=255)
                                    cGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATc                                                                                                   >  2:3473921/1‑140 (MQ=255)
                                     gCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATcc                                                                                                  <  1:1974878/140‑1 (MQ=255)
                                      cAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATCGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcg                                                                                                 <  2:4088459/140‑1 (MQ=255)
                                       aGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcgc                                                                                                <  2:2856175/140‑1 (MQ=255)
                                       aGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCcgc                                                                                                <  2:3418688/140‑1 (MQ=255)
                                         taatacAACCCCCGGCCATAAAAGCACCACTATTGGGGCTGACCACTGCAGTCAAAAAGCCCCCAAAAGCCTCGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGGGAGACTAATTATCCGCgg                                                                                              <  2:4452497/140‑1 (MQ=255)
                                         taataaAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCgg                                                                                              <  2:2527689/140‑1 (MQ=255)
                                            taaAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTg                                                                                           >  2:1554115/1‑140 (MQ=255)
                                            taaAACCCCAGGCAATAAAAGCACCAATATTGGGCATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTg                                                                                           <  2:4794301/140‑1 (MQ=255)
                                                   ccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGt                                                                                    >  1:3393350/1‑140 (MQ=255)
                                                   ccAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGt                                                                                    >  1:5006815/1‑140 (MQ=255)
                                                    cAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTc                                                                                   >  1:4169562/1‑140 (MQ=255)
                                                     aGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTcc                                                                                  >  2:4973815/1‑140 (MQ=255)
                                                     aGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGATTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTcc                                                                                  >  1:3632858/1‑140 (MQ=255)
                                                      ggCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCg                                                                                 >  1:821963/1‑140 (MQ=255)
                                                               aGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGtt                                                                        >  1:4935574/1‑140 (MQ=255)
                                                                        atTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCtgatg                                                               <  1:1358650/140‑1 (MQ=255)
                                                                          tGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTgatgat                                                             <  2:2731100/140‑1 (MQ=255)
                                                                          tGGGGATGACAATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTgatgat                                                             <  1:3472136/140‑1 (MQ=255)
                                                                           ggggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                            <  1:1878206/140‑1 (MQ=255)
                                                                           ggggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                            <  1:559866/140‑1 (MQ=255)
                                                                           ggggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGatgatg                                                            <  2:2142681/140‑1 (MQ=255)
                                                                           ggagATGACCATTGCAATCAAAACGCCCCCCACAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATCATATTTTCCTGTAGAGGACAGAGTAATTATCCGCGGCTGCTAATGTCCGCTAATTGTTTTTCTGatgatg                                                            <  2:4242289/137‑1 (MQ=255)
                                                                           cgcgATGACCATTGCAGTCAGAATGCCCCCAAAAGCCTGGCCCGTTGCGCGAGCATACTTGTTTTCCATAATATTAGCCTGGAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAAGTGTTTGTCTGatgatg                                                            <  2:4853226/137‑1 (MQ=255)
                                                                            gggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAgagga                                                                                                                <  2:2981933/87‑1 (MQ=255)
                                                                            gggATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAAGATTATCCGGTAGAGGAGAGAGTAAGTATCCGCGGCTGATAATGTCCGCTAATTGTTTGTCTGATGATGc                                                           >  2:3715914/1‑140 (MQ=255)
                                                                                tGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTgct                                                       >  2:2215660/1‑138 (MQ=255)
                                                                                 gACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAAGATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTa                                                      >  1:2815283/1‑140 (MQ=255)
                                                                                   ccATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAag                                                    <  1:4482987/140‑1 (MQ=255)
                                                                                     aTTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAagag                                                  <  2:3990954/140‑1 (MQ=255)
                                                                                            tCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCg                                           <  1:1106900/140‑1 (MQ=255)
                                                                                              aaaaaGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAg                                         >  2:4751103/1‑140 (MQ=255)
                                                                                                   gCCCCCAAATGCCTGGACCTTTGCACGAGCCGACTTGTTTTCCATACTATTATCCGGCGGCGGAGAGAGTAGTTTTTCGCGGCTGATATTGTCCGTTAATTGTTTTTCTGATGATGCTGTTTAGAGTTGTTCGAGTCttt                                    <  2:1877948/140‑1 (MQ=255)
                                                                                                      cccAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATttctt                                 <  1:1733453/140‑1 (MQ=255)
                                                                                                        cAAAAGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATttcttct                               <  1:2080133/140‑1 (MQ=255)
                                                                                                           aaGCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCg                            <  2:1824292/140‑1 (MQ=255)
                                                                                                             gCCTGGACCTTTGCACGAGCAGCCTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATTATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGca                          <  2:774096/140‑1 (MQ=255)
                                                                                                             gCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGGGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGca                          <  1:1575880/140‑1 (MQ=255)
                                                                                                             gCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGATTTGTTCGAGTATTTCTTCTTCGca                          <  1:650844/140‑1 (MQ=255)
                                                                                                             gCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGca                          <  1:4862019/140‑1 (MQ=255)
                                                                                                             gCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGca                          <  2:144520/140‑1 (MQ=255)
                                                                                                             gCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGca                          <  1:1672384/140‑1 (MQ=255)
                                                                                                             gCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGca                          <  2:1170518/140‑1 (MQ=255)
                                                                                                             gCCTGGACCTTTGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGca                          <  2:5056350/140‑1 (MQ=255)
                                                                                                                       ttGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCa                >  2:1040847/1‑140 (MQ=255)
                                                                                                                       ttGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCa                >  1:23265/1‑140 (MQ=255)
                                                                                                                        tGCACGAGGAGGCTTGTTTTCCATAATATTATCCTGTAGCGGAGAGAGTAATTATCGGCGGCTGATAATGTGCGCTAATTGTGGTTCTTATGATGCTGTTGAGAGTTGGTCGAGTATGTCTTCTTCGCACAGTAAGTCAc               >  2:1633960/1‑140 (MQ=255)
                                                                                                                        tGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCAc               >  2:3559802/1‑140 (MQ=255)
                                                                                                                        tGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCAc               >  1:3907322/1‑140 (MQ=255)
                                                                                                                        tGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCAc               >  1:3153852/1‑140 (MQ=255)
                                                                                                                        tGCACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCAc               >  1:1051210/1‑140 (MQ=255)
                                                                                                                          cACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACtt             <  1:3309620/140‑1 (MQ=255)
                                                                                                                          cACGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACtt             <  1:2290962/140‑1 (MQ=255)
                                                                                                                           aCGAGCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAA‑TATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTaa           <  1:4032427/140‑1 (MQ=255)
                                                                                                                               gCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGc        <  2:1754468/140‑1 (MQ=255)
                                                                                                                               gCAGACTTGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGc        >  1:932043/1‑140 (MQ=255)
                                                                                                                                     ttGTTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAAt  <  2:4339456/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
ATGTTCGTTAGGCAGCCAACCGGTGGGAATAAATAACGCAGTAATAAAACCCCAGGCAATAAAAGCACCAATATTGGGGATGACCATTGCAGTCAAAAAGCCCCCAAAAGCCTGGACCTTTGCACGAGCAGACTTGCTTTCCATAATATTATCCTGTAGAGGAGAGAGTAATTATCCGCGGCTGATAATGTCCGCTAATTGTTTTTCTGATGATGCTGTTAAGAGTTGTTCGAGTATTTCTTCTTCGCACAGTAATTCACTTAACGCCTGAAT  >  NZ_CP025268/3081020‑3081292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑