BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,085,924	A→G	C60R (TGT→CGT)	speB ←	agmatinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,085,924	0	A	G	99.7%	1083.7 / NA	321	C60R (TGT→CGT)	speB	agmatinase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (155/165); total (155/166)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 321 total aligned reads displayed.

AGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAGG  >  NZ_CP025268/3085788‑3086055

aGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGAt                                                                                                                                  <  1:119768/140‑1 (MQ=255)

aGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGAt                                                                                                                                  <  2:1522273/140‑1 (MQ=255)

aGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGAt                                                                                                                                  <  2:1391435/140‑1 (MQ=255)

aGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGAt                                                                                                                                  <  2:1549288/140‑1 (MQ=255)

aGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGAt                                                                                                                                  <  2:3756775/140‑1 (MQ=255)

aGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGAt                                                                                                                                  <  1:2113209/140‑1 (MQ=255)

                    ggCATCGCCAAAGGCATATACCAGCTCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGac                                                                                                              <  1:2165674/140‑1 (MQ=255)

                        tCGCCAAAGGCATATACCAGATCGCCGCAGTCCCCGACGTTCAGACGCTCAAGCATGTCGAAAGTCCACGGGAAGCGGGTGTGTTCCCAGGCCAGATGCGTCGAAACCTGAGGGAGCACGGCCGGACCGTGGCgggcaca                                                                                                          >  2:3074622/1‑134 (MQ=255)

                        tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGaccacc                                                                                                          >  1:4103592/1‑140 (MQ=255)

                        tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGaccacc                                                                                                          >  1:4570438/1‑140 (MQ=255)

                        tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGaccacc                                                                                                          >  2:2817906/1‑140 (MQ=255)

                        tCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGaccacc                                                                                                          >  2:4315432/1‑140 (MQ=255)

                           ccAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGc                                                                                                       <  1:240821/140‑1 (MQ=255)

                           ccAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGc                                                                                                       <  2:1755236/140‑1 (MQ=255)

                           ccAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGc                                                                                                       <  1:2904649/140‑1 (MQ=255)

                               aGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGa                                                                                                   >  1:3705175/1‑140 (MQ=255)

                                ggaatagACGAGAGCGCCGGGGGCGACGGCGTGCAGAGGCTCAGGCATGTCGAAGTTCCACGGGAAGCGGTTGTGTTCCCGGGGCAGATTCGTCGAAACCGGACGGATCGCTGCCGGACCGTGGCGACGGCCCGCACGAc                                                                                                  <  2:3680164/137‑1 (MQ=255)

                                ggCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGAc                                                                                                  <  2:4198074/140‑1 (MQ=255)

                                ggCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGAc                                                                                                  <  1:4623638/140‑1 (MQ=255)

                                    tataCCAGCTCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCCGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGa                                                                                              <  1:2566343/140‑1 (MQ=255)

                                    tataCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGa                                                                                              <  2:3518168/140‑1 (MQ=255)

                                    tataCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGa                                                                                              >  2:3937158/1‑140 (MQ=255)

                                     ataCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGaa                                                                                             >  2:2738851/1‑140 (MQ=255)

                                     ataCCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGaa                                                                                             <  1:1789959/140‑1 (MQ=255)

                                      taCCAGATCGCCGCAGTCCACGACGTTCAGACGCGCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAg                                                                                            <  2:2275535/140‑1 (MQ=255)

                                         cAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTgg                                                                                         <  2:4648926/140‑1 (MQ=255)

                                         cAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTgg                                                                                         <  1:4887804/140‑1 (MQ=255)

                                         cAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTgg                                                                                         <  1:5105822/140‑1 (MQ=255)

                                           gATCGCCGCAGTGCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCAAGGCCAGATTCGTCGAACCCTGACGGATCGCAGCCGGACCGTGGCGACCCCCCGCACGCCCAGAAGTGAcc                                                                                       >  2:5035864/1‑140 (MQ=255)

                                           gATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGcc                                                                                       <  1:2083114/140‑1 (MQ=255)

                                           gATCGCCGCAGTCCACGACGTCCAGACGCTCGCGCATGGCGAAATGCCACGGGAAGCGGTTGTGTTGCCAGGCCAGATCCGTCGAAACCTGACGGAGCGCTGCCTGACCGTGGCGACCACCCGCCCGACCAGAAGTGGcg                                                                                       >  2:1850891/1‑139 (MQ=255)

                                            aTCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCa                                                                                      >  1:4551261/1‑140 (MQ=255)

                                              cgccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCata                                                                                    >  1:2712411/1‑140 (MQ=255)

                                              cgccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGGGGCCata                                                                                    >  2:251943/1‑140 (MQ=255)

                                                ccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCTACCACCCGCACGACCAGCAGTGGCCATATc                                                                                  >  2:2645491/1‑140 (MQ=255)

                                                ccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATc                                                                                  >  2:2503601/1‑140 (MQ=255)

                                                ccgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACCACCAGAAGTGGCCATATc                                                                                  >  1:3183323/1‑140 (MQ=255)

                                                ccgcAGTCCACGACGTTCAGACGCCCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACGCGCACGACGAGAAGTGGCCATATc                                                                                  >  1:1623245/1‑140 (MQ=255)

                                                 cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCAc                                                                                                     >  1:2245959/1‑120 (MQ=255)

                                                 cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCAc                                                                                                     <  2:2245959/120‑1 (MQ=255)

                                                 cgcAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCg                                                                                 >  1:1394885/1‑140 (MQ=255)

                                                   cAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGaa                                                                               <  1:3926066/140‑1 (MQ=255)

                                                   cAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGAGCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGaa                                                                               <  1:3052558/140‑1 (MQ=255)

                                                      tCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCTGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACgg                                                                            <  2:4605020/140‑1 (MQ=255)

                                                        cACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCa                                                                          <  2:2217918/140‑1 (MQ=255)

                                                        cACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCa                                                                          <  1:4096067/140‑1 (MQ=255)

                                                           gccgTTCAGAAGCTCACGCATGTCGACATTACACGGGAAGCGGTTGTGTTCCCAGGCCACAGTCGTCGAAACCTGACGGTGCGCTGCCGGACCGTGGCGAACACCCGCAGGACCCGAAGTGGCCATATCGAACGGCACGc                                                                       <  2:1364257/138‑1 (MQ=255)

                                                           gacgTTCAGACGCTCACGCGTGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGCTCGCTGCCGGACCGTGGCGACCCCCCGCACGACCAGAAGTGGCCATATCGAACGGCACGc                                                                       <  1:2646916/140‑1 (MQ=255)

                                                           gacgTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGc                                                                       <  1:2315205/140‑1 (MQ=255)

                                                            acgTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGcc                                                                      <  2:2578724/140‑1 (MQ=255)

                                                            acgTTCAGACGCTCACGCATGTCCAAATTCCACGGGAAGCGGTTGTGTCTCCAGGCCAGGTTCGTCGAAACCGGACGGACCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTCGCCATCTCGAACGGCACGcc                                                                      <  2:4077413/140‑1 (MQ=255)

                                                               ttCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCCGt                                                                   >  2:609044/1‑140 (MQ=255)

                                                               ttCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGt                                                                   <  2:1457499/140‑1 (MQ=255)

                                                                taaGACTCTCACTCATGTCGCGGTGCCCACGGAGGCGTTTGTGTTCCTAGCCCCGATTGGTGCAAACCTGACGTATCCCTGCCGGGCCGTGGCGACCGCCCGCACGACCAGAAGTGGCCATATCGCACGGCGCGCCAGTa                                                                  <  2:788297/138‑1 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCCGTa                                                                  >  2:1947322/1‑140 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                  <  1:3434112/140‑1 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                  <  2:1782204/140‑1 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                  >  1:2017015/1‑140 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTa                                                                  <  1:785620/140‑1 (MQ=255)

                                                                tCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTCGCCATATCGAACGGCACGCCAGTa                                                                  >  2:3315725/1‑140 (MQ=255)

                                                                    aCGCTCACGCATGTCGAAGTTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCCCGACCAGAAGGGGCCATATCGAAAGGCACGCCCATAATCa                                                              >  2:4452568/1‑140 (MQ=255)

                                                                     cGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAc                                                             <  2:163619/140‑1 (MQ=255)

                                                                      gCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAcc                                                            <  1:2751713/140‑1 (MQ=255)

                                                                       cTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAGCCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAccc                                                           <  1:3833060/140‑1 (MQ=255)

                                                                       cTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGTCACGCCAGGAAGCAccc                                                           >  2:2776183/1‑140 (MQ=255)

                                                                       cTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAccc                                                           >  1:4730485/1‑140 (MQ=255)

                                                                       cTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAccc                                                           >  1:4165604/1‑140 (MQ=255)

                                                                       cTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAACCAccc                                                           >  1:3527807/1‑140 (MQ=255)

                                                                       cTCACGCATGTCGAAATTCCACAGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCAccc                                                           >  2:4927243/1‑140 (MQ=255)

                                                                        tcgcccATGTCCACATTCCACGGGAAGCGGTTGTGTCCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGCACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCa                                                          <  2:1100617/135‑1 (MQ=255)

                                                                        tCACGCATGTTGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGCTTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCa                                                          <  2:547132/140‑1 (MQ=255)

                                                                         cACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAg                                                         >  1:533656/1‑140 (MQ=255)

                                                                         cACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAg                                                         >  1:4912191/1‑140 (MQ=255)

                                                                         cACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAg                                                         >  1:4778516/1‑140 (MQ=255)

                                                                         cACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAg                                                         >  2:3354186/1‑140 (MQ=255)

                                                                         cACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACCGCACGCCAGTAATCACCCAg                                                         >  2:206734/1‑140 (MQ=255)

                                                                               tGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCa                                                   <  2:3195863/140‑1 (MQ=255)

                                                                                gTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCAt                                                  <  1:246208/140‑1 (MQ=255)

                                                                                gTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCAt                                                  <  1:739362/140‑1 (MQ=255)

                                                                                gTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCAt                                                  <  2:1914166/140‑1 (MQ=255)

                                                                                 tCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATc                                                 >  1:919063/1‑140 (MQ=255)

                                                                                  cGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCg                                                >  1:3610552/1‑140 (MQ=255)

                                                                                         ccACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCAt                                         <  1:3099714/140‑1 (MQ=255)

                                                                                              ggAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGc                                    <  2:4816493/140‑1 (MQ=255)

                                                                                                         tgtTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGGAGTTCAt                         >  1:3944413/1‑140 (MQ=255)

                                                                                                         tgtTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCAt                         >  1:877946/1‑140 (MQ=255)

                                                                                                         tgtTCCCAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGGGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCAGCATGGCGGTCATACGTCTGGAGGTTCAt                         >  2:3238062/1‑140 (MQ=255)

                                                                                                             cccAGGCCAGATTCGTCGAAACCTGACAGATCGCTGACGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGc                     <  2:1293833/140‑1 (MQ=255)

                                                                                                               cAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAg                   <  2:1993229/140‑1 (MQ=255)

                                                                                                               cAGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAg                   <  2:4718366/140‑1 (MQ=255)

                                                                                                                aGGCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGGCATACGGCTGGAAGTTCATCGGCAgg                  >  2:3047122/1‑140 (MQ=255)

                                                                                                                  gCCAGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCg                >  2:2368877/1‑140 (MQ=255)

                                                                                                                     aGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTaa             >  1:1927817/1‑140 (MQ=255)

                                                                                                                     aGATTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTaa             >  1:1297285/1‑140 (MQ=255)

                                                                                                                      gATTCGCCGAAACCTGACGGATCGCTGCCCGACCGTGGCGACCAACCGCACGACCAGAAGCCGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTaaa            <  1:331765/140‑1 (MQ=255)

                                                                                                                       aTTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGTATTTCATCGGCAGGCGTaaaa           >  2:3904968/1‑140 (MQ=255)

                                                                                                                       aTTCGTCGAAACCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACAGCTGGAAGTTCATACGCAGGAGCaaaa           >  2:4100032/1‑140 (MQ=255)

                                                                                                                                aaCCTGCCGGGTCGCTGGCGGACCGTGGAGACCACCCGCACGACCAGACGTGGCCATATCCAACCGCACGCCCGTAATCTCCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCCTCGGCAGGCGTAAAAAACCAAAgg  <  2:290514/140‑1 (MQ=255)

                                                                                                                                aaCCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAgg  <  1:672722/140‑1 (MQ=255)

                                                                                                                                aaCCTGACGGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAgg  <  1:4280045/140‑1 (MQ=255)

AGCTTTTCGCTCATCTCACGGGCATCGCCAAAGGCATATACCAGATCGCCGCAGTCCACGACGTTCAGACGCTCACGCATGTCGAAATTCCACGGGAAGCGGTTGTGTTCCCAGGCCAGATTCGTCGAAACCTGACAGATCGCTGCCGGACCGTGGCGACCACCCGCACGACCAGAAGTGGCCATATCGAACGGCACGCCAGTAATCACCCAGTCTGCATCGCTGTCATACGGCTGGAAGTTCATCGGCAGGCGTAAAAAACCAAAGG  >  NZ_CP025268/3085788‑3086055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`