BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,179,162	T→C	141 (TAA→TAG)	yqiB ←	DUF1249 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,179,162	0	T	C	99.3%	931.5 / NA	286	141 (TAA→TAG)	yqiB	DUF1249 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (151/133); minor base A (0/2); total (151/135)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 286 total aligned reads displayed.

TGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGCGA  >  NZ_CP025268/3179026‑3179297

 gTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGTCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCGTAGGTTTCACGACGCtaat                                                                                                                                     >  1:523423/1‑140 (MQ=255)

 gTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaat                                                                                                                                     >  1:2431559/1‑140 (MQ=255)

     aCCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAAc                                                                                                                                 >  2:1166234/1‑140 (MQ=255)

          tAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGaa                                                                                                                            >  2:3925467/1‑140 (MQ=255)

           aaCAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAAt                                                                                                                           >  2:1000972/1‑140 (MQ=255)

            aCAGGGCTTCGTGCTGTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCCCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCTTTAGGTTTCACGAAGCTAATAAACCGGAATc                                                                                                                          >  2:2392074/1‑140 (MQ=255)

               gggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGcc                                                                                                                       <  1:1716964/140‑1 (MQ=255)

               gggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGcc                                                                                                                       <  1:2647832/140‑1 (MQ=255)

                ggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCa                                                                                                                      <  2:1712652/140‑1 (MQ=255)

                  cttcttGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGCCTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTGCACGACGCTAATAAACCGGAATCGCCATc                                                                                                                    <  2:2863618/140‑1 (MQ=255)

                  cTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATc                                                                                                                    <  2:2975262/140‑1 (MQ=255)

                     cGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCGTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCt                                                                                                                 >  2:2459490/1‑140 (MQ=255)

                     cGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCt                                                                                                                 <  1:3668348/140‑1 (MQ=255)

                     cGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCt                                                                                                                 <  1:2815004/140‑1 (MQ=255)

                     cGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCAGCGCt                                                                                                                 >  2:3188548/1‑140 (MQ=255)

                       ttcTTGTGTGCAAACAGGTGAGTGTCAGTAATTTGTCAAAGCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTACCAGGCTTTCCAAATGGTGTCCTTAGTGTTCACGACGCTAATAAACCGGAATCGCGATCGCTcc                                                                                                               <  2:4886313/138‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                               <  1:3026380/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                               <  2:4365552/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                               <  2:3987578/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                               <  1:2152615/140‑1 (MQ=255)

                       tGCTTGTGTGCAAACACGTGCGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCCGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTGTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                               <  1:2838629/140‑1 (MQ=255)

                         cTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCAt                                                                                                             <  1:146992/140‑1 (MQ=255)

                            ttGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                          <  1:1396201/140‑1 (MQ=255)

                            ttGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                          <  1:1567770/140‑1 (MQ=255)

                            ttGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                          >  1:1196099/1‑140 (MQ=255)

                               tgCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTa                                                                                                       >  2:1720202/1‑140 (MQ=255)

                                 cAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                     >  1:2106724/1‑140 (MQ=255)

                                 cAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                     <  2:5068072/140‑1 (MQ=255)

                                 cAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                     <  2:3334808/140‑1 (MQ=255)

                                   aaCAGGTGAGTGTCGGTCATTTGTACAATCCTGACTCTGGCCGCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACa                                                                                                   <  2:1601668/140‑1 (MQ=255)

                                   aaCAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACa                                                                                                   <  2:2294314/140‑1 (MQ=255)

                                    aCAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAg                                                                                                  >  2:3860474/1‑140 (MQ=255)

                                       ggTGAGTGTCGGTCATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCAAAATGGTGTCCTTAGGTTTCACGACGCTAATAACCCGGAATCGCCATCGCTCAATGTGCTAAACAGTAt                                                                                               <  1:1757106/140‑1 (MQ=255)

                                         tGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCg                                                                                             >  1:1908801/1‑140 (MQ=255)

                                          gAGTGTCGGTAATTTGTAAACTCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTTCAAATGGTGTCATTAGGTTTCACGACGCTAATAAATTGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGc                                                                                            <  1:316273/140‑1 (MQ=255)

                                           aGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCa                                                                                           >  2:4871761/1‑140 (MQ=255)

                                           aGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCa                                                                                           >  1:2721260/1‑140 (MQ=255)

                                               tCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCa                                                                                       <  1:4778463/140‑1 (MQ=255)

                                                 ggTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGt                                                                                     <  2:4618158/140‑1 (MQ=255)

                                                 ggTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGt                                                                                     <  2:3151872/140‑1 (MQ=255)

                                                 ggTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGt                                                                                     <  1:4288269/140‑1 (MQ=255)

                                                 ggTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGt                                                                                     <  2:1528493/140‑1 (MQ=255)

                                                 ggTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGt                                                                                     <  2:1825742/140‑1 (MQ=255)

                                                  gTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTc                                                                                    >  1:4977314/1‑140 (MQ=255)

                                                  gTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTc                                                                                    >  1:1601401/1‑140 (MQ=255)

                                                     aTTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCATTCCGc                                                                                 >  2:4478201/1‑140 (MQ=255)

                                                           aaaaTCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTaaaaa                                                                           <  2:221046/140‑1 (MQ=255)

                                                                ccTGACTCTGGCCTCACCAGCCAGAGGCAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGAt                                                                      <  2:612950/140‑1 (MQ=255)

                                                                  tGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTa                                                                    >  1:1335902/1‑140 (MQ=255)

                                                                        tGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGa                                                              >  1:234930/1‑140 (MQ=255)

                                                                         ggCCTCACCAGCCAGCGGCAGGGTTAACAGGCTTTCCAACTGGTGTCCTCAGGTTTCACGCCGCTAATACACCGGCATCGCCCGCGCTCCATGTGCTAACCAGTATCGCAACCAGTCCGCTAAAACCTGATCAATTTGac                                                             >  2:280578/1‑139 (MQ=255)

                                                                          gCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATg                                                            >  1:4642648/1‑140 (MQ=255)

                                                                          gCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATg                                                            <  2:4339846/140‑1 (MQ=255)

                                                                              cACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCttt                                                        >  1:4515693/1‑140 (MQ=255)

                                                                               aCCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCtttt                                                       >  1:740918/1‑140 (MQ=255)

                                                                               aCCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCtttt                                                       >  2:2607644/1‑140 (MQ=255)

                                                                               aCCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTGAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCtttt                                                       >  2:4661776/1‑140 (MQ=255)

                                                                                ccagccagAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTtc                                                      >  2:1072653/1‑140 (MQ=255)

                                                                                  agcaagAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTtcgt                                                    <  2:1433925/140‑1 (MQ=255)

                                                                                     cagAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTcgc                                                 <  1:3287344/140‑1 (MQ=255)

                                                                                      agagGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTcgcg                                                <  1:4000513/140‑1 (MQ=255)

                                                                                             gggTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGc                                         <  2:5003247/140‑1 (MQ=255)

                                                                                              ggTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCa                                        >  2:268120/1‑140 (MQ=255)

                                                                                                  aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACtt                                    >  2:1636440/1‑140 (MQ=255)

                                                                                                  aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACtt                                    >  1:917897/1‑140 (MQ=255)

                                                                                                  aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACtt                                    >  1:4102138/1‑140 (MQ=255)

                                                                                                       gCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttat                               <  1:3400333/140‑1 (MQ=255)

                                                                                                        cTTTCCAAATGGTGTCCTTAGTCTTCACGCAGCTAATAAACCGGAATCGCCATCGCTCGATGTCCTAAACAGTATCGCAACCAGTCCGCTAATACCTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt                              <  2:4065343/140‑1 (MQ=255)

                                                                                                        cTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttctt                              >  2:830805/1‑140 (MQ=255)

                                                                                                        cTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt                              >  1:4310895/1‑140 (MQ=255)

                                                                                                        cTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt                              <  1:4429706/140‑1 (MQ=255)

                                                                                                         tttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatta                             >  1:1037275/1‑140 (MQ=255)

                                                                                                         tttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatta                             >  2:4345237/1‑140 (MQ=255)

                                                                                                          ttCCAATTGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                            <  2:634501/140‑1 (MQ=255)

                                                                                                          ttCCAAATTGTGTCCTTAGGTTTCACGACGCTAATAAACCGTAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTAGTCGCGTTGATGCAACTTTTTATTAg                            <  1:421460/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAAGTTTTTATTAg                            >  2:133523/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                            <  2:3367571/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                            >  2:1199735/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                            <  1:1143935/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                            <  1:79664/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                            >  1:128810/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                            <  1:2156273/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                            <  1:3868003/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTGTTATTAg                            >  1:123346/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAAGCGCCATCGCTCCATGTGCTAAGCAGTCTCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATGAg                            <  2:328959/140‑1 (MQ=255)

                                                                                                            ccAACTGCTGCCCTTAGGTTTCACGACGCTAATCAACCGGAACCGCCATAGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTACTTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                          <  2:1101712/140‑1 (MQ=255)

                                                                                                            ccAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                          <  1:657960/140‑1 (MQ=255)

                                                                                                               aaTGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATaa                       <  2:4204978/140‑1 (MQ=255)

                                                                                                                  ggTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCa                    >  1:3410211/1‑140 (MQ=255)

                                                                                                                     gtCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATAc                 >  1:2939319/1‑140 (MQ=255)

                                                                                                                     gtCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATAc                 >  1:2559243/1‑140 (MQ=255)

                                                                                                                     gtCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATAc                 >  2:2024345/1‑140 (MQ=255)

                                                                                                                     gtCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATAc                 >  2:3552021/1‑140 (MQ=255)

                                                                                                                      tCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTGGTCGCGTTGATGCAACTTTTTATTAGGATAATCATaac                >  2:3032191/1‑138 (MQ=255)

                                                                                                                      tCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTAATTGATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATAcc                >  1:3632087/1‑140 (MQ=255)

                                                                                                                               ttCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAAGAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATCCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGa       <  2:379236/140‑1 (MQ=255)

                                                                                                                                tgacgacgCTAATAAACCGGAATCGCCATCGCGCCGTGTGCTAAACAGTATCCCAACCAGTCAGCGAAAGACTAATTATTTTGATACTTTTAGTCGCGCTGATCCCACAGTCTATTAGGATAATCATACCGCGCTTTGaa      >  2:3652378/3‑140 (MQ=255)

                                                                                                                                tCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGaa      >  1:2217374/1‑140 (MQ=255)

                                                                                                                                  acgacgCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGc    >  1:5068965/1‑140 (MQ=255)

                                                                                                                                  acgacgCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCAGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGc    >  2:727074/1‑140 (MQ=255)

TGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGCGA  >  NZ_CP025268/3179026‑3179297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`