Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,272,617 C→T A276T (GCG→ACG)  garR ← 2‑hydroxy‑3‑oxopropionate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,272,6170CT99.3% 1090.6 / NA 305A276T (GCG→ACG) garR2‑hydroxy‑3‑oxopropionate reductase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (140/162);  minor base G (1/1);  total (142/163)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

CGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCG  >  NZ_CP025268/3272478‑3272751
                                                                                                                                           |                                                                                                                                      
cGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTCTGATCcgccgc                                                                                                                                        >  2:3615733/1‑140 (MQ=255)
   cGGAGGGCGTTTCTCTTATTCGGCCTGGATTGCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTcc                                                                                                                                     >  2:3886433/1‑140 (MQ=255)
      acggccgttCTCTTATTCGGTCTGGATTCCAGTCCCGGATTGCAATACGCAATACGGGCACGACGTCACTGACGAGTAACTTCGACTTTAGCCACTTTTTCGTGGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTaa                                                                                                                                  <  2:570070/133‑1 (MQ=255)
      aGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTaa                                                                                                                                  >  1:4787750/1‑140 (MQ=255)
        ggCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAAc                                                                                                                                <  1:5058580/140‑1 (MQ=255)
          cGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCTTCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTGTTTCGTAGTAGCACGCCAGGGCGCTATGATCCTCCGTTCCTAAACCa                                                                                                                              >  2:309338/1‑140 (MQ=255)
           gTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCAt                                                                                                                             >  1:2249126/1‑140 (MQ=255)
             ttCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCt                                                                                                                           >  1:3964114/1‑140 (MQ=255)
              tctcTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTg                                                                                                                          <  2:4387713/140‑1 (MQ=255)
                  ttATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCg                                                                                                                      <  2:3185809/140‑1 (MQ=255)
                  ttATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCg                                                                                                                      <  2:497404/140‑1 (MQ=255)
                    aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                                                                                                                    <  2:4272929/140‑1 (MQ=255)
                    aTTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCAATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCGAGTTTTTCTTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                                                                                                                    >  2:1978851/1‑140 (MQ=255)
                         gCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGcc                                                                                                               <  1:2921330/140‑1 (MQ=255)
                          ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTGTGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                                                                                                              >  2:4415799/1‑140 (MQ=255)
                          ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                                                                                                              >  1:2036352/1‑140 (MQ=255)
                          ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                                                                                                              >  1:2561543/1‑140 (MQ=255)
                          ccTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCt                                                                                                              >  1:3714211/1‑140 (MQ=255)
                             ggATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGca                                                                                                           <  1:2947626/140‑1 (MQ=255)
                              gATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcat                                                                                                          >  2:2999359/1‑140 (MQ=255)
                              gATTCCACGCCCGGATTGCAACACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTCCGCCTGTTTTTCGTAGTAGCACGCCAGGGCGCTATAATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcat                                                                                                          >  2:401526/1‑140 (MQ=255)
                               aTTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                                         <  1:194559/140‑1 (MQ=255)
                               aTTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                                         <  1:1607959/140‑1 (MQ=255)
                               aTTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                                         <  1:1465191/140‑1 (MQ=255)
                               aTTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatc                                                                                                         <  1:3555086/140‑1 (MQ=255)
                                 tCCAGCCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTAGCCAGTTTTTCGTAGTAGCACGCCAGGGCGTTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatcat                                                                                                       <  2:4497015/140‑1 (MQ=255)
                                 tCCACGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGcatcat                                                                                                       <  1:4419596/140‑1 (MQ=255)
                                    aGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCa                                                                                                                    <  2:879436/124‑1 (MQ=255)
                                    aGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCAtctc                                                                                                    >  1:3805191/1‑140 (MQ=255)
                                     ggCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTcc                                                                                                   <  1:1372568/140‑1 (MQ=255)
                                       cccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCAt                                                                                                 <  1:2292080/140‑1 (MQ=255)
                                       cccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCAt                                                                                                 <  1:2204884/140‑1 (MQ=255)
                                       cccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCAt                                                                                                 <  2:1223724/140‑1 (MQ=255)
                                        ccGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATa                                                                                                >  1:3099272/1‑140 (MQ=255)
                                          ggAGTGCAATACGCCATTCGGGCACGACGCCATTATAGAGTAACGTCGACCTTCGCCAGCTCTTCGGAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTATACCCTCTACTCGCGGTGCATGCCTCATCTCCATAAc                                                                                              >  2:159236/1‑140 (MQ=255)
                                            aTTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCg                                                                                            <  2:4649781/140‑1 (MQ=255)
                                            aTTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCg                                                                                            >  2:5001350/1‑140 (MQ=255)
                                                cAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGc                                                                                        >  1:1061911/1‑140 (MQ=255)
                                                cAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGc                                                                                        >  1:4081361/1‑140 (MQ=255)
                                                     cGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGTCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGa                                                                                   >  1:3640232/1‑140 (MQ=255)
                                                      gCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAg                                                                                  >  2:1535710/1‑140 (MQ=255)
                                                        cATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCTCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACGTCAGCTGTGAGCg                                                                                >  2:4378035/1‑140 (MQ=255)
                                                        cATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCg                                                                                >  1:2451945/1‑140 (MQ=255)
                                                         aTCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCgg                                                                               <  2:1268112/140‑1 (MQ=255)
                                                         aTCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCgg                                                                               <  2:3706154/140‑1 (MQ=255)
                                                         aTCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCgg                                                                               <  2:3799547/140‑1 (MQ=255)
                                                         aTCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCgg                                                                               <  1:691872/140‑1 (MQ=255)
                                                          tCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGc                                                                              >  2:2899847/1‑140 (MQ=255)
                                                          tCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGc                                                                              >  2:2370241/1‑140 (MQ=255)
                                                          tCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGc                                                                              >  2:411813/1‑140 (MQ=255)
                                                            cGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCTAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAg                                                                            <  1:2851211/140‑1 (MQ=255)
                                                             gggCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTCGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCCGt                                                                           >  2:2214742/1‑140 (MQ=255)
                                                               gCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTGTATCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTtg                                                                         >  2:2285638/1‑140 (MQ=255)
                                                                     cgTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGcc                                                                   >  1:3399556/1‑140 (MQ=255)
                                                                       tCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGa                                                                 <  1:4944195/140‑1 (MQ=255)
                                                                        cATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGAc                                                                <  1:1796368/140‑1 (MQ=255)
                                                                             atgattAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                                           <  2:4730161/135‑1 (MQ=255)
                                                                             aCGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                                           >  2:203134/1‑140 (MQ=255)
                                                                             aCGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                                           <  1:956676/140‑1 (MQ=255)
                                                                             aCGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                                           <  2:3532061/140‑1 (MQ=255)
                                                                             aCGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                                           <  1:4875015/140‑1 (MQ=255)
                                                                                          aCTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAg                                              >  1:1972205/1‑140 (MQ=255)
                                                                                              tCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCa                                          <  2:1882571/140‑1 (MQ=255)
                                                                                              tCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCa                                          <  2:3346021/140‑1 (MQ=255)
                                                                                               cGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCAt                                         <  1:2389256/140‑1 (MQ=255)
                                                                                                   aGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGc                                     <  2:2580619/140‑1 (MQ=255)
                                                                                                    gTTCTTCCTAGTAGCACGCCAGTGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGcc                                    <  2:25499/140‑1 (MQ=255)
                                                                                                     tttttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCa                                   <  1:41792/140‑1 (MQ=255)
                                                                                                        ttCGTCCTAGCACGCCAGGGCGATAGGATCCGCCGTTCCTAAACCATCTGCTCGCACTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                                <  2:4578638/140‑1 (MQ=255)
                                                                                                        ttCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGAt                                <  1:4543891/140‑1 (MQ=255)
                                                                                                             agtagCACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCCGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTa                           <  1:2727301/140‑1 (MQ=255)
                                                                                                             agtagCACGCCAGGGCGCTATGATCCGCCGTTACTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTa                           <  1:4772024/140‑1 (MQ=255)
                                                                                                             agtagCACGCCAAGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTa                           <  2:3589178/140‑1 (MQ=255)
                                                                                                                  cACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg                      <  1:3821811/140‑1 (MQ=255)
                                                                                                                  cACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg                      <  1:1147417/140‑1 (MQ=255)
                                                                                                                  cACGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATg                      <  2:4333332/140‑1 (MQ=255)
                                                                                                                   aCGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGc                     <  1:3010714/140‑1 (MQ=255)
                                                                                                                   aCGCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGc                     <  2:2679876/140‑1 (MQ=255)
                                                                                                                     gCCAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAg                   <  2:4764964/140‑1 (MQ=255)
                                                                                                                     gCCAGGGCGCTATGAGCCGCCGTTCCTAAACCATCTGCGCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAg                   <  2:1161582/140‑1 (MQ=255)
                                                                                                                       cAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGAt                 <  1:2100949/140‑1 (MQ=255)
                                                                                                                       cAGGGCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGAt                 >  2:4911504/1‑140 (MQ=255)
                                                                                                                       cAGGGCGCTATGATCCGCCGTACCTAAACCATCTGCTCACAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGACCCTTAATATGCAGAt                 <  1:2008509/140‑1 (MQ=255)
                                                                                                                         gggCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                                           <  2:760431/96‑1 (MQ=255)
                                                                                                                         gggCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGt                                                           >  1:760431/1‑96 (MQ=255)
                                                                                                                         gggCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCa               >  1:1829971/1‑140 (MQ=255)
                                                                                                                         gggCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCa               >  1:4672165/1‑140 (MQ=255)
                                                                                                                         gggCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCa               >  1:3468912/1‑140 (MQ=255)
                                                                                                                          ggCGCTATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCaa              >  2:220518/1‑140 (MQ=255)
                                                                                                                               tATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCTCATTCGCCAGATCCTTAATATGCAGATCAATACgg         >  2:3155665/1‑140 (MQ=255)
                                                                                                                               tATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACgg         >  2:3772077/1‑140 (MQ=255)
                                                                                                                               tATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACgg         >  1:1574197/1‑140 (MQ=255)
                                                                                                                               tATGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCGTTGAGCGGCAGTTGCGCGCGGACGCCGGGAGAAGTATCCAGCGCATTCGCCAGATGCTTAATATGCCGATCAATACgg         >  2:1825433/1‑140 (MQ=255)
                                                                                                                                 tGATCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGaa       >  1:319741/1‑140 (MQ=255)
                                                                                                                                   aTCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGc     >  2:1710808/1‑140 (MQ=255)
                                                                                                                                    tCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGcc    <  2:705599/140‑1 (MQ=255)
                                                                                                                                    tCCGCCGTTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTCGTGCGCCGCCGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGcc    >  2:954792/1‑140 (MQ=255)
                                                                                                                                      cgccgtTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATGACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCg  >  1:4363021/1‑140 (MQ=255)
                                                                                                                                      cgccgtTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCg  >  1:4282659/1‑140 (MQ=255)
                                                                                                                                      cgccgtTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCg  >  1:83187/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                      
CGCCGGAGGGCGTTTCTCTTATTCGGCCTGGATTCCAGGCCCGGATTGCAATACGCCATCCGGGCACGACGTCATTAACGAGTAACTTCGACTTTCGCCAGTTTTTCGTAGTAGCACGCCAGGGCGCTATGATCCGCCGCTCCTAAACCATCTGCTCGCAGTGCCTGCATCATCTCCATAACCGCAGCTGTGAGCGGCAGTTGTGCGCCGACGCCGTGAGAAGTATCCAGCGCATTCGCCAGATCCTTAATATGCAGATCAATACGGAAGCCCG  >  NZ_CP025268/3272478‑3272751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑