BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	344,042	A→G	272 (TAA→TAG)	CXP41_RS01800 →	protein YahL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	344,042	0	A	G	99.0%	1037.7 / NA	314	272 (TAA→TAG)	CXP41_RS01800	protein YahL
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (200/110); minor base T (2/1); total (203/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

TATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGC  >  NZ_CP025268/343906‑344177

          tGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTACAATCCGCTAAACTCTTTAGTTCAGAAAGGGtt                                                                                                                            >  2:2868751/1‑140 (MQ=255)

           gTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTc                                                                                                                           <  1:3238012/140‑1 (MQ=255)

                 gACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTg                                                                                                                     >  2:3066284/1‑140 (MQ=255)

                    gTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGATGGCAGGAATTAGGGGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCGACCTGCaa                                                                                                                  >  2:3945005/1‑140 (MQ=255)

                      aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAGATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgt                                                                                                                >  2:2937823/1‑140 (MQ=255)

                      aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgt                                                                                                                >  2:244529/1‑140 (MQ=255)

                      aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgt                                                                                                                >  1:5035899/1‑140 (MQ=255)

                      aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATCATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgt                                                                                                                >  1:1210066/1‑140 (MQ=255)

                      aaaaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGGGCAGGAATTAGGTGACGGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACGTGCAAgt                                                                                                                >  2:4535782/1‑140 (MQ=255)

                        aaaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgttg                                                                                                              >  2:1085043/1‑140 (MQ=255)

                         aaCATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATCGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAgttgt                                                                                                             <  2:4161349/140‑1 (MQ=255)

                           aaTACAGAGATGATGCGCGACTAAATGAATGGATATCTAATAGACTGCAGGAATTAGCTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTa                                                                                                           <  2:3989471/139‑1 (MQ=255)

                             tACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                         <  1:2985709/140‑1 (MQ=255)

                             tACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTAtt                                                                                                         <  1:569788/140‑1 (MQ=255)

                                    atgatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTg                                                                                                  >  2:2247603/1‑140 (MQ=255)

                                    atgatgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTg                                                                                                  >  1:4723478/1‑140 (MQ=255)

                                       atgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTTCAAGTTGTTATTCGACCTgaga                                                                                               >  2:2108830/1‑140 (MQ=255)

                                       atgCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTgaga                                                                                               >  2:1441945/1‑140 (MQ=255)

                                         gcgcGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGt                                                                                             <  1:3306361/140‑1 (MQ=255)

                                          cgcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCGCGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                            <  1:3809447/140‑1 (MQ=255)

                                          cgcgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTg                                                                                            <  1:1019830/140‑1 (MQ=255)

                                            cgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGaa                                                                                          >  1:4994032/1‑140 (MQ=255)

                                            cgATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGaa                                                                                          >  1:2495688/1‑140 (MQ=255)

                                                    tgaatgGATATCTAATCGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCTCGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTTCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATg                                                                                  <  1:2830156/140‑1 (MQ=255)

                                                     gaatgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAGATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGc                                                                                 <  1:4693045/140‑1 (MQ=255)

                                                       atgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAg                                                                               >  1:1110098/1‑140 (MQ=255)

                                                        tgGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAgg                                                                              >  2:1887710/1‑140 (MQ=255)

                                                            tatCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAt                                                                          <  2:762874/140‑1 (MQ=255)

                                                             atCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATTCAGGTAGTc                                                                         >  2:3189408/1‑140 (MQ=255)

                                                             atCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATc                                                                         >  1:3937871/1‑140 (MQ=255)

                                                               cTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAt                                                                       <  2:4642086/140‑1 (MQ=255)

                                                               cTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAt                                                                       >  1:3508242/1‑140 (MQ=255)

                                                                tAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCAtt                                                                      <  1:2063361/140‑1 (MQ=255)

                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCACGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  <  1:1858121/140‑1 (MQ=255)

                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  <  2:786521/140‑1 (MQ=255)

                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  <  2:3759574/140‑1 (MQ=255)

                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  <  2:259023/140‑1 (MQ=255)

                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  <  1:2122568/140‑1 (MQ=255)

                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  <  1:724665/140‑1 (MQ=255)

                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  <  1:2700671/140‑1 (MQ=255)

                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  <  1:3617926/140‑1 (MQ=255)

                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTGGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  <  1:1553229/140‑1 (MQ=255)

                                                                    agagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAACGGTTACACCTGCAAGCTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  <  2:2565349/140‑1 (MQ=255)

                                                                     gagTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatc                                                                 >  2:1344968/1‑140 (MQ=255)

                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTTCACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                    >  2:2307803/1‑136 (MQ=255)

                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTTCACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTc                                                                    <  1:2307803/136‑1 (MQ=255)

                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatct                                                                >  2:3389613/1‑140 (MQ=255)

                                                                      agTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  <  2:471623/138‑1 (MQ=255)

                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAGAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAAGCATTTCatctgt                                                              >  1:4350735/1‑140 (MQ=255)

                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatgtgt                                                              >  2:633458/1‑140 (MQ=255)

                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgt                                                              >  1:2739754/1‑140 (MQ=255)

                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgt                                                              >  2:4922857/1‑140 (MQ=255)

                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgt                                                              >  1:2748197/1‑140 (MQ=255)

                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgt                                                              >  1:1506068/1‑140 (MQ=255)

                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgt                                                              >  2:4968216/1‑140 (MQ=255)

                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgt                                                              >  2:1237486/1‑140 (MQ=255)

                                                                        tGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgt                                                              >  1:385014/1‑140 (MQ=255)

                                                                         gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                             <  2:1485401/140‑1 (MQ=255)

                                                                         gCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTATTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgta                                                             >  2:483669/1‑140 (MQ=255)

                                                                          gaGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTGGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCGTTTCatctggac                                                            >  1:3249445/2‑137 (MQ=255)

                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTATTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                            >  1:4786518/1‑140 (MQ=255)

                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                            >  1:1445825/1‑140 (MQ=255)

                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                            >  2:385659/1‑140 (MQ=255)

                                                                          cAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCat                                                                  >  1:84609/1‑134 (MQ=255)

                                                                          cAGGAATTAGGGGAAAGATATTATCCCGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGACAGGGTTCCACCTGCCAGTTGTTATTCCTCCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtat                                                            <  2:1823477/140‑1 (MQ=255)

                                                                                ttAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtatatctgt                                                      >  2:4343906/1‑140 (MQ=255)

                                                                                ttAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtatatctgt                                                      >  1:1614242/1‑140 (MQ=255)

                                                                                 tAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatctgtatatctgta                                                     >  1:1154385/1‑140 (MQ=255)

                                                                                  aGGTGACAGATATTCTCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCGGAAAGGGTTCCACCTGCCAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCatcggtatatctttat                                                    >  2:267067/1‑140 (MQ=255)

                                                                                       aCAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCGaa                                               >  1:1965690/1‑140 (MQ=255)

                                                                                             attatCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCa                                         >  1:3596086/1‑140 (MQ=255)

                                                                                                     gTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtct                                 >  1:3132355/1‑140 (MQ=255)

                                                                                                     gTAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtct                                 >  1:2545241/1‑140 (MQ=255)

                                                                                                      tAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCGAtctc                                >  2:519127/1‑140 (MQ=255)

                                                                                                      tAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtctc                                >  1:3371515/1‑140 (MQ=255)

                                                                                                      tAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtctc                                >  2:5064821/1‑140 (MQ=255)

                                                                                                      tAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtctc                                >  2:3481890/1‑140 (MQ=255)

                                                                                                      tAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtctc                                >  2:582010/1‑140 (MQ=255)

                                                                                                      tAAATAAGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTAGTTATTCCACCTGAGCGTGAATCGCAATGCAGGTAAACATTTCATCTGTATATCTGTATGCCAACTGGCATTCCAtatc                                >  1:3025620/1‑140 (MQ=255)

                                                                                                           aaGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCttttt                           >  2:1649017/1‑140 (MQ=255)

                                                                                                           aaGCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCttttt                           >  1:3073778/1‑140 (MQ=255)

                                                                                                             gCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTc                         >  2:475714/1‑140 (MQ=255)

                                                                                                             gCACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTc                         >  1:3768351/1‑140 (MQ=255)

                                                                                                              taCGTTAAAATCCGCTACACTATTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCAACCTGGGAGTGAATCACAATGCAGGTAATCATTTCATCGGTATATCCGTAGGCCAACTGGCATTCCATCTCGTTTTTCg                        >  2:4226112/2‑140 (MQ=255)

                                                                                                              cACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGCGTGAATCGCAATGCAGGTAATCCTTTCCTCTTTATATCTGTATGCCCACTGGCATTCCATCTCTTTTTCCg                        <  2:2735254/140‑1 (MQ=255)

                                                                                                              cACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCg                        >  1:1244037/1‑140 (MQ=255)

                                                                                                              cACGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCg                        >  1:1689506/1‑140 (MQ=255)

                                                                                                                cGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGcc                      >  1:3465687/1‑140 (MQ=255)

                                                                                                                cGTTAAAATCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGcc                      >  1:2760911/1‑140 (MQ=255)

                                                                                                                    aaaaTCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGata                  <  2:3566455/140‑1 (MQ=255)

                                                                                                                    aaaaTCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGata                  <  1:5001251/140‑1 (MQ=255)

                                                                                                                    aaaaTCCGCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATCCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGata                  <  1:4719363/140‑1 (MQ=255)

                                                                                                                           gCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTttgtt           >  1:3718429/1‑140 (MQ=255)

                                                                                                                           gCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTttgtt           >  2:1130066/1‑140 (MQ=255)

                                                                                                                           gCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTttgtt           >  1:404696/1‑140 (MQ=255)

                                                                                                                           gCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTAGCCGATATTttgtt           >  2:1941905/1‑140 (MQ=255)

                                                                                                                           gCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCCGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTttgtt           >  1:3350282/1‑140 (MQ=255)

                                                                                                                           gCTACACTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATAATTTCATCTGTATATCTGGATGCCAACTGGCATTCCATCTCTTTTTTCGCAGAGATCttgtt           >  2:4490062/1‑140 (MQ=255)

                                                                                                                              acacTCTTTAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTtgtg        <  1:2207543/140‑1 (MQ=255)

                                                                                                                                    tttAGTTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGc  >  2:2915458/1‑140 (MQ=255)

TATTGCTCTCTGTTCTGGACGTAAAAACATACAGAGATGATGCGCGATTAAATGAATGGATATCTAATAGAGTGCAGGAATTAGGTGACAGATATTATCCTGTAAATAAGCACGTTAAAATCCGCTACACTCTTTAATTCAGAAAGGGTTCCACCTGCAAGTTGTTATTCCACCTGAGAGTGAATCGCAATGCAGGTAATCATTTCATCTGTATATCTGTATGCCAACTGGCATTCCATCTCTTTTTTCGCCGATATTTTGTTGTGATCGGC  >  NZ_CP025268/343906‑344177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`