BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,308,725	T→C	295 (TAA→TAG)	nlpI ←	lipoprotein NlpI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,308,725	0	T	C	99.7%	1182.3 / NA	356	295 (TAA→TAG)	nlpI	lipoprotein NlpI
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (160/194); total (160/196)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 356 total aligned reads displayed.

CTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCA  >  NZ_CP025268/3308589‑3308861

cTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTAt                                                                                                                                       >  1:1972986/1‑140 (MQ=255)

cTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTAt                                                                                                                                       >  1:3693796/1‑140 (MQ=255)

cTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTAt                                                                                                                                       >  2:2462279/1‑140 (MQ=255)

 tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTAtt                                                                                                                                      >  2:1899091/1‑140 (MQ=255)

 tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTAtt                                                                                                                                      >  2:3763993/1‑140 (MQ=255)

 tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTAtt                                                                                                                                      >  2:2633983/1‑140 (MQ=255)

 tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTAtt                                                                                                                                      >  1:1568910/1‑140 (MQ=255)

 tcatcaTGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGCATTAACGAACAACAACGCCCTCACCCGTTAAGGTGGTGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTAtt                                                                                                                                      >  2:3058849/1‑140 (MQ=255)

  catcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTg                                                                                                                                     <  1:379244/140‑1 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGc                                                                                                                                    >  1:4775528/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGc                                                                                                                                    >  1:946792/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGc                                                                                                                                    >  1:1961869/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGc                                                                                                                                    >  2:4970157/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGc                                                                                                                                    >  1:2790574/1‑140 (MQ=255)

   atcatGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGc                                                                                                                                    >  1:2449495/1‑140 (MQ=255)

     catGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTg                                                                                                                                  >  1:741315/1‑140 (MQ=255)

     catGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTg                                                                                                                                  >  1:2654791/1‑140 (MQ=255)

     catGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTg                                                                                                                                  >  1:2369914/1‑140 (MQ=255)

       tGAAATTGCTCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGt                                                                                                                                <  2:1379546/140‑1 (MQ=255)

       tGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGt                                                                                                                                <  2:1234354/140‑1 (MQ=255)

       tGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGt                                                                                                                                <  1:2144697/140‑1 (MQ=255)

       tGAAATCGATCAGCAATTTTCAATGCAAAGCGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTTAGGTGAGGTCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGt                                                                                                                                <  2:4003615/140‑1 (MQ=255)

          aaTTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCg                                                                                                                             <  2:3036953/140‑1 (MQ=255)

          aaTTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCg                                                                                                                             >  1:4233020/1‑140 (MQ=255)

          aaTTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCg                                                                                                                             >  1:1796693/1‑140 (MQ=255)

              gATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTc                                                                                                                         <  2:2301980/140‑1 (MQ=255)

                tCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTg                                                                                                                       <  1:4670187/140‑1 (MQ=255)

                 cAGCAATTTTCATTGAAAAGTGTGAACCGGCTTCAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTAATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGc                                                                                                                      <  2:316695/140‑1 (MQ=255)

                     acttttCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAgg                                                                                                                  <  2:4817317/138‑1 (MQ=255)

                           cATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACACCGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                            <  1:3474982/140‑1 (MQ=255)

                           cATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCt                                                                                                            <  2:2810772/140‑1 (MQ=255)

                            aTTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCtt                                                                                                           >  1:4025982/1‑140 (MQ=255)

                                 aaaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                      <  1:851382/140‑1 (MQ=255)

                                 aaaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                      <  2:91877/140‑1 (MQ=255)

                                 aaaGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTcc                                                                                                      <  2:3626955/140‑1 (MQ=255)

                                          aCCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAgg                                                                                             <  2:1919456/140‑1 (MQ=255)

                                           ccGGCTCAAAGTAGGTGTATTAACGAACAACCACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGa                                                                                            <  2:478273/140‑1 (MQ=255)

                                              gCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCTGCTATTGCTGGTCCGATTCTGCCAGGTAATCTTGGTCCTGGCCCAGGAGCg                                                                                         <  1:3059881/140‑1 (MQ=255)

                                              gCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                         <  1:3923543/140‑1 (MQ=255)

                                              gCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                         <  2:3280331/140‑1 (MQ=255)

                                              gCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCg                                                                                         <  2:4591434/140‑1 (MQ=255)

                                              gCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCAGCGTGGTCCTGGCCCAGGAGCg                                                                                         >  2:4154733/1‑140 (MQ=255)

                                               cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGa                                                                                        >  2:1136481/1‑140 (MQ=255)

                                               cTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGa                                                                                        >  1:691621/1‑140 (MQ=255)

                                                tCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt                                                                                       <  2:2519875/140‑1 (MQ=255)

                                                tCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGAt                                                                                       <  1:3445798/140‑1 (MQ=255)

                                                         gtgtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaac                                                                              >  1:4866145/1‑140 (MQ=255)

                                                          tgtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaaca                                                                             >  1:1498782/1‑140 (MQ=255)

                                                          tgtATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCcaaca                                                                             <  1:1683231/140‑1 (MQ=255)

                                                          tgaaTTAACGACCCACAGCGCCATAACCGGTAAAGGCGAGCGCAATGAAAAAAGGCTGCGGGCTGATGTGTCCGTCGGGTAAGCCTGGGTCGCGTCGGCCAGGCCAACGGGGTCCTTGGCCAGGAGCGATAATTCcaaca                                                                             <  2:1553037/137‑1 (MQ=255)

                                                            tATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAAt                                                                           >  1:927931/1‑140 (MQ=255)

                                                                aaCGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGt                                                                       >  2:3972113/1‑140 (MQ=255)

                                                                    aacaacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCg                                                                   >  2:721802/1‑140 (MQ=255)

                                                                    aacaacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCg                                                                   >  2:3857049/1‑140 (MQ=255)

                                                                      caacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGt                                                                 >  2:1837075/1‑140 (MQ=255)

                                                                       aacaacGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGCTTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTg                                                                <  1:3856245/140‑1 (MQ=255)

                                                                            cGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGACGTGTACGTCAGCTATTGCTGGGACGATTCTGCCAGGGCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaa                                                           <  2:1337870/140‑1 (MQ=255)

                                                                              cgcTCACCCGTTATGGTGATGGCAATCAAAAAAGACTACGGGCTGCTGTTTACGTCAGCTATTTCTGGTCCGATTCTGCCAGGTCATCTTGGTCCCGGCCCAGGAGCCATACTTCCAACAATGCGTATCGGTGCTccaca                                                         <  2:3896057/138‑1 (MQ=255)

                                                                              cccTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTcaaca                                                         <  1:1590468/140‑1 (MQ=255)

                                                                                   aCCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGtt                                                    >  1:2322239/1‑140 (MQ=255)

                                                                                   aCCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGGCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCGGGGTCCTGGCCCAGGAGCGAGAAGTCCTACAATGCGTATCGTTGCTCAAGAAAGtt                                                    >  1:2891339/1‑140 (MQ=255)

                                                                                     ccGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTAt                                                  <  1:4317911/140‑1 (MQ=255)

                                                                                        ttAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                                               >  1:2753876/1‑140 (MQ=255)

                                                                                        ttAAGGTGATGGCAATCAAAAAAGATTACGGGCTGAGGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGCGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGaa                                               >  1:1436998/1‑140 (MQ=255)

                                                                                          aaGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACg                                             <  1:4423935/140‑1 (MQ=255)

                                                                                          aaGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACg                                             <  2:3336339/140‑1 (MQ=255)

                                                                                               gagggCAATCAAAAAAGAGTACGGGCTGATGTGTACGTCAGCGATTGCTGGTCCGAGTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGGTTGCAACAAAGTTATGAACgttgtt                                        >  2:1462375/4‑140 (MQ=255)

                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACgttgtt                                        >  2:2349173/1‑140 (MQ=255)

                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACgttgtt                                        >  2:845518/1‑140 (MQ=255)

                                                                                               gATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACgttgtt                                        >  1:2979339/1‑140 (MQ=255)

                                                                                                  ggCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGc                                     >  1:412987/1‑140 (MQ=255)

                                                                                                     aaTCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAAc                                  >  2:1978664/1‑140 (MQ=255)

                                                                                                      aTCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAAcc                                 >  1:5080123/1‑140 (MQ=255)

                                                                                                       tcagagaaGATGGCGGGCTGATGTGTACGTCAGCTATTGCGGGGCCTGTTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCGAACATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                                <  2:2753717/134‑1 (MQ=255)

                                                                                                       tCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCGTCTTGGTCCTGGGCCACGAGCGATAATTCCAACATTGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                                <  2:1159203/140‑1 (MQ=255)

                                                                                                       tCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                                <  1:1178291/140‑1 (MQ=255)

                                                                                                       tCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                                <  1:1208782/140‑1 (MQ=255)

                                                                                                       tCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                                <  2:4739304/140‑1 (MQ=255)

                                                                                                       tCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                                <  2:1705870/140‑1 (MQ=255)

                                                                                                       tCAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATAGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCg                                <  2:1053856/140‑1 (MQ=255)

                                                                                                        cAAAAAAGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGc                               >  1:558430/1‑140 (MQ=255)

                                                                                                         aaaaaaGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGcc                              <  1:3350394/140‑1 (MQ=255)

                                                                                                            aaaGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGt                           <  2:590208/140‑1 (MQ=255)

                                                                                                            aaaGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGt                           <  1:2109234/140‑1 (MQ=255)

                                                                                                            aaaGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGt                           <  2:5062049/140‑1 (MQ=255)

                                                                                                            aaaGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGt                           <  1:3185487/140‑1 (MQ=255)

                                                                                                              aGATTACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGttt                         <  2:755958/140‑1 (MQ=255)

                                                                                                                aTTACGGGCTGATGTGTACGTCAGCTATTGTTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCGCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGa                       >  1:1097368/1‑140 (MQ=255)

                                                                                                                 ttACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa                      <  2:2200548/140‑1 (MQ=255)

                                                                                                                 ttACGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa                      <  2:3502172/140‑1 (MQ=255)

                                                                                                                 ttACGGGCTGATGCGTACGTCAGCTATTGCTGGTCCGATTCTGCCTGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAAGAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGaa                      <  2:4411327/140‑1 (MQ=255)

                                                                                                                    cGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAg                   <  1:2009929/140‑1 (MQ=255)

                                                                                                                    cGGGCTGATGTGTACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAg                   <  1:2541311/140‑1 (MQ=255)

                                                                                                                              tgtACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGACCAGTGCCGTGgcg         <  2:4239288/140‑1 (MQ=255)

                                                                                                                              tgtACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgcg         <  1:1246848/140‑1 (MQ=255)

                                                                                                                              tgtACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgcg         <  1:4995930/140‑1 (MQ=255)

                                                                                                                              tgtACGTCAGCTATGGCGGGTCCGATTCTGCCAGGTGATCTTGGTCGTGGCGCAGGAGCGAGAATTCCAACAATGCGTATCGGTGCTCAACACAGTTATGAACGTGGTTGGCAACCGCCAGTTTTAAAAGTGCCGTGgcg         <  2:5042594/140‑1 (MQ=255)

                                                                                                                               gtACGTCAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGgcgc        >  2:2012843/1‑140 (MQ=255)

                                                                                                                                     cAGCTATTTCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCTTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCa  >  2:541262/1‑140 (MQ=255)

                                                                                                                                     cAGCTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCa  >  2:2121696/1‑140 (MQ=255)

CTCATCATGAAATTGATCAGCAATTTTCATTGAAAAGTGTGAACCGGCTCAAAGTAGGTGTATTAACGAACAACAACGCCCTCACCCGTTAAGGTGATGGCAATCAAAAAAGATTACGGGCTGATGTGTACGTCAGTTATTGCTGGTCCGATTCTGCCAGGTCATCTTGGTCCTGGCCCAGGAGCGATAATTCCAACAATGCGTATCGGTGCTCAACAAAGTTATGAACGTTGTTGGCAACCGCCAGTTTGAACAGTGCCGTGGCGCTGTCCA  >  NZ_CP025268/3308589‑3308861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`