BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,386,542	T→C	91 (TAA→TAG)	yhcO ←	barstar family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,386,542	0	T	C	99.5%	618.7 / NA	188	91 (TAA→TAG)	yhcO	barstar family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (128/59); minor base A (1/0); total (129/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 188 total aligned reads displayed.

AAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGG  >  NZ_CP025268/3386409‑3386673

aaaGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCTACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGa                                                                                                                               >  1:3327913/1‑140 (MQ=255)

  aGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGCCGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACg                                                                                                                             <  2:734468/140‑1 (MQ=255)

    gcgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGaa                                                                                                                           <  2:1133917/140‑1 (MQ=255)

    gcgcAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGaa                                                                                                                           <  2:3518907/140‑1 (MQ=255)

        aaCGGCCTACCAGATTACTGAAGCTGGTAGCGGTGACACCTGGCACGCTACGGCTGAAGTGTACAAATCAACGCTCAGCGTCTTGTCCGACGATATTGCGCCCGGTTCGGGGGCTTTTTTTTGCGATAAGGAAGAACAtt                                                                                                                       >  2:4504237/1‑140 (MQ=255)

         aCGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTa                                                                                                                      >  1:484565/1‑140 (MQ=255)

           ggCCTACCAGATTACTGACGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTaaa                                                                                                                    <  1:1240929/140‑1 (MQ=255)

                    gATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGCTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATg                                                                                                           <  2:4022019/140‑1 (MQ=255)

                       tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGccc                                                                                                        >  1:1838120/1‑140 (MQ=255)

                       tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGccc                                                                                                        >  1:4799195/1‑140 (MQ=255)

                       tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGccc                                                                                                        >  2:317324/1‑140 (MQ=255)

                       tACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGGACAAATAAACCCTCATCGTCTTGTCCGACGATCTGGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACACTAAACCGCaacttacc                                                                                                        >  2:2822820/1‑134 (MQ=255)

                          tGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGCTCGGGGGCTTTTTTTTGCGCTAATGACGAACATGAAAACGCAAATGCCCTTc                                                                                                     >  2:3641678/1‑140 (MQ=255)

                           gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAGCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                    <  2:4362806/140‑1 (MQ=255)

                           gAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTcc                                                                                                    <  1:1941508/140‑1 (MQ=255)

                            aaGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCa                                                                                                   >  2:383146/1‑140 (MQ=255)

                             aGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAg                                                                                                  <  2:228631/140‑1 (MQ=255)

                              gCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAgg                                                                                                 >  2:1491600/1‑139 (MQ=255)

                               cgcGTAGCGGCGACACCTGGCACGCTACGGCTGAACGGTACAGATAAAACCTCATTGTCTTGTCCGACGGTATTGCCCCCGGTGCGGGGGCATTTTTTTGCGCTAATGACTAACATTAAAACGCAAATGCCCCTCCAgtt                                                                                                >  2:2700500/3‑138 (MQ=255)

                               cTCGTAGCGGTGACACCTGGCACGCTCCGGCTGAACTGTACCACTAACCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                <  2:1875415/140‑1 (MQ=255)

                               cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                <  2:484565/140‑1 (MQ=255)

                               cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                >  2:420427/1‑140 (MQ=255)

                               cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                >  1:1017345/1‑140 (MQ=255)

                               cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGcc                                                                                                <  1:1384247/140‑2 (MQ=255)

                               cTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAAGACCCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGct                                                                                                >  1:167397/1‑140 (MQ=255)

                                tCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGctc                                                                                               <  1:2913775/140‑1 (MQ=255)

                                   tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTc                                                                                            >  1:2123581/1‑140 (MQ=255)

                                   tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTc                                                                                            >  1:4966058/1‑140 (MQ=255)

                                   tAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTc                                                                                            >  1:4965860/1‑140 (MQ=255)

                                     gCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCct                                                                                          <  2:1202232/140‑1 (MQ=255)

                                        gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                       >  2:5003257/1‑140 (MQ=255)

                                        gTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTg                                                                                       >  2:2965797/1‑140 (MQ=255)

                                             acCTGGCACGCTACGGCTGAACTGTACAAATCAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCtca                                                                                  <  2:4729115/140‑1 (MQ=255)

                                                 ggCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaa                                                                              >  2:3568710/1‑140 (MQ=255)

                                                 ggCACGCTACGGCTGAACTGGACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGTTTTTGTTTGTTCTAATGACTAACCTTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCaa                                                                              >  1:3122401/1‑140 (MQ=255)

                                                     cgatACGGCTGAACTGTACAATTAACCCCTCATCGTCTTGTCCGCCGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                          <  2:1517379/137‑1 (MQ=255)

                                                     cGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                          <  1:2619939/140‑1 (MQ=255)

                                                     cGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAg                                                                          <  1:3175973/140‑1 (MQ=255)

                                                       cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTGCAGCTCTTCCTCTGCTTCATCAAACAGCa                                                                        >  2:4290155/1‑140 (MQ=255)

                                                       cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCa                                                                        >  1:2760789/1‑140 (MQ=255)

                                                       cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCa                                                                        >  2:1352363/1‑140 (MQ=255)

                                                       cTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCa                                                                        >  1:2443693/1‑140 (MQ=255)

                                                        tACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGGTATTGCCCCCGGTTCGGGGGCTTTTTTTTTCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCaa                                                                       >  2:3136810/1‑140 (MQ=255)

                                                        tACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCaa                                                                       >  2:2960055/1‑140 (MQ=255)

                                                              tGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTaa                                                                 <  1:836825/140‑1 (MQ=255)

                                                                aaCTGTACAAATACACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAcg                                                               >  1:173639/1‑140 (MQ=255)

                                                                     tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTGTGCCTCATCAAACAGCAATATTAACGCGCCa                                                          >  1:1473408/1‑140 (MQ=255)

                                                                     tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                                                          >  2:3498470/1‑140 (MQ=255)

                                                                     tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                                                          >  1:2021960/1‑140 (MQ=255)

                                                                     tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                                                          >  2:1760753/1‑140 (MQ=255)

                                                                     tACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGGTCGGGGGCTTTGTTTTGCGCTAATGACGAACATTAAAAGGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATAGTAACGCGCCa                                                          >  2:2586592/1‑140 (MQ=255)

                                                                     tACAAATAAACCCTCATCGTCTTGTACGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCa                                                          >  2:4584624/1‑140 (MQ=255)

                                                                      aCAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTCGCGCTAATGACGAACATTAAAAGGCAAATGCCCTTGCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCaa                                                         >  2:3059266/1‑140 (MQ=255)

                                                                          aTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTaa                                                                                                                     <  2:2954579/76‑1 (MQ=255)

                                                                          aTAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTaa                                                                                                                     >  1:2954579/1‑76 (MQ=255)

                                                                            aaaCCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGc                                                   <  1:1801709/140‑1 (MQ=255)

                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                >  2:824298/1‑140 (MQ=255)

                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                >  1:1005983/1‑140 (MQ=255)

                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                >  1:2190073/1‑140 (MQ=255)

                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                >  1:2225257/1‑140 (MQ=255)

                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                >  2:1578735/1‑140 (MQ=255)

                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                >  1:3624464/1‑140 (MQ=255)

                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                >  2:4056987/1‑140 (MQ=255)

                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                >  1:634107/1‑140 (MQ=255)

                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGAc                                                >  1:5072511/1‑140 (MQ=255)

                                                                               cccTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAAAGCGCCAAAAGGGCGAc                                                >  2:4863167/1‑140 (MQ=255)

                                                                                    aTCGTCTTGTCCGACGATCTTGCCCCCGGTTCTGGGGCTTTGTTTTGCGCTAATGACGAACCTTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTAGTCAAACAGCAATATTAACGCGCGAAAACGGCGACGCGtt                                           >  2:2056599/1‑140 (MQ=255)

                                                                                    aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtt                                           >  1:4634788/1‑140 (MQ=255)

                                                                                    aTCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtt                                           >  1:348206/1‑140 (MQ=255)

                                                                                      cGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGtttt                                         <  1:4254757/140‑1 (MQ=255)

                                                                                         cTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctc                                      >  1:2882315/1‑140 (MQ=255)

                                                                                          ttGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTtctct                                     >  1:1660080/1‑140 (MQ=255)

                                                                                            gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTcc                                   >  1:4059603/1‑140 (MQ=255)

                                                                                            gTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTGTCTCTcc                                   >  2:3005164/1‑140 (MQ=255)

                                                                                               cgacgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAg                                <  1:149717/140‑1 (MQ=255)

                                                                                                gacgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGa                               <  2:3207175/140‑1 (MQ=255)

                                                                                                 acgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTTCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTTCCTCATCAAACAGCAAAATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGAt                              >  2:1455262/1‑140 (MQ=255)

                                                                                                 acgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGAt                              >  2:2345860/1‑140 (MQ=255)

                                                                                                  cgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCACTTGCCCTTCAAGGTCTTCCTCTGCGTCATAAAACAGCAATATTAACGCGCCAAAACGGCGACACGTTTTCTCTCCCAGTTg                             >  1:3603916/1‑140 (MQ=255)

                                                                                                  cgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATg                             >  2:3434954/1‑140 (MQ=255)

                                                                                                  cgaTATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATg                             >  1:48230/1‑140 (MQ=255)

                                                                                                              cGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTACAACGCAAATGCCCTCCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAt                 >  2:2252118/1‑140 (MQ=255)

                                                                                                              cGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAt                 <  2:3406820/140‑1 (MQ=255)

                                                                                                              cGGTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAt                 <  2:3119991/140‑1 (MQ=255)

                                                                                                               ggTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAAc                          >  2:3247511/1‑130 (MQ=255)

                                                                                                               ggTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtc                <  1:1041457/140‑1 (MQ=255)

                                                                                                               ggTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtc                >  2:3985375/1‑140 (MQ=255)

                                                                                                               ggTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtc                <  1:4874695/140‑1 (MQ=255)

                                                                                                               ggTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTATCTCTCCCAGATGAACAAATTCAAtc                >  2:402127/1‑140 (MQ=255)

                                                                                                                gTTCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAAtcc               >  2:2137725/1‑139 (MQ=255)

                                                                                                                 ttCGGGGGCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTACAGCGCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCACTCCCATATGAACAAATTCaagtgc              >  2:2208592/1‑136 (MQ=255)

                                                                                                                    gggggCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAg           >  1:1719919/1‑140 (MQ=255)

                                                                                                                     ggggCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGt          >  1:2378577/1‑140 (MQ=255)

                                                                                                                     ggggCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGt          >  1:3021052/1‑140 (MQ=255)

                                                                                                                        gCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt       <  2:3780863/140‑1 (MQ=255)

                                                                                                                        gCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt       <  1:4386981/140‑1 (MQ=255)

                                                                                                                        gCTTTTTTTTGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGt       <  2:548067/140‑1 (MQ=255)

                                                                                                                          ttttttttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAg     <  1:420427/140‑1 (MQ=255)

                                                                                                                           tttttttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGc    >  2:2761330/1‑140 (MQ=255)

                                                                                                                             tttttGCGCTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCgg  <  2:4418783/140‑1 (MQ=255)

AAAGGCGCAACGGCCTACCAGATTACTGAAGCTCGTAGCGGTGACACCTGGCACGCTACGGCTGAACTGTACAAATAAACCCTCATCGTCTTGTCCGACGATATTGCCCCCGGTTCGGGGGCTTTTTTTTGCGTTAATGACGAACATTAAAACGCAAATGCCCTTCCAGCTCTTCCTCTGCCTCATCAAACAGCAATATTAACGCGCCAAAACGGCGACGCGTTTTCTCTCCCAGATGAACAAATTCAATCTCAAGTGGTAGCGG  >  NZ_CP025268/3386409‑3386673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`