BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,434,451	A→G	T26A (ACA→GCA)	def →	peptide deformylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,434,451	0	A	G	99.7%	1062.7 / NA	321	T26A (ACA→GCA)	def	peptide deformylase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (130/190); minor base T (0/1); total (130/191)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 321 total aligned reads displayed.

GGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATACAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGC  >  NZ_CP025268/3434314‑3434586

 gggATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAg                                                                                                                                      <  2:2115882/140‑1 (MQ=255)

    aTTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGAGTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGaaa                                                                                                                                   >  1:1728335/1‑140 (MQ=255)

        gTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCa                                                                                                                               >  1:3218154/1‑140 (MQ=255)

           tagaatagaaGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGGAGAAGAAGGGAATGCAGAAATTCAGCg                                                                                                                            >  1:2233229/1‑140 (MQ=255)

              aatagaaGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAt                                                                                                                         <  1:978079/140‑1 (MQ=255)

               atagaaGAAATAATCTTTCTAATTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAtc                                                                                                                        <  2:2821650/140‑1 (MQ=255)

                tagaaGAAATAATCTTTCTAACTCCTGCACACATCGCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATGCAGCGTAtcg                                                                                                                       <  2:4967804/140‑1 (MQ=255)

                   aagaaATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGACGAAGTGAATGCAGAAATTCAGCGTAtcgtcg                                                                                                                    >  1:734846/1‑140 (MQ=255)

                   aagaaATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAtcgtcg                                                                                                                    >  2:1783351/1‑140 (MQ=255)

                   aagaaATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAtcgtcg                                                                                                                    >  1:5769/1‑140 (MQ=255)

                   aagaaATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAtcgtcg                                                                                                                    >  1:753702/1‑140 (MQ=255)

                   aagaaATAATCTTTCTAACTCCTGAACACATCTCTGGAGATGTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTAtcgtcg                                                                                                                    >  1:1601452/1‑140 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTTGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGGCgattat                                                                                                               <  1:650154/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  2:3347211/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               >  1:4542640/1‑140 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  1:3450164/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  1:1916230/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  2:5068972/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTGAACACACCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTACACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  2:2730198/140‑1 (MQ=255)

                        ataatCTTTCTAACTCCTAAACACATCTCTGGAGATTTATGTCAGATTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  2:1499990/140‑1 (MQ=255)

                        ataatCTTTATAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCgatgat                                                                                                               <  1:1662369/140‑1 (MQ=255)

                             cTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGtt                                                                                                          >  1:4578870/1‑140 (MQ=255)

                                 ttAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCCGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCgag                                                                                                      <  2:3716773/139‑1 (MQ=255)

                                 cTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCgag                                                                                                      <  1:3309919/140‑1 (MQ=255)

                                 cTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCgag                                                                                                      <  2:1640183/140‑1 (MQ=255)

                                 cTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACCAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCgag                                                                                                      <  2:4499928/140‑1 (MQ=255)

                                    ccggcTGAACACATGCCTGGAGGTTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCAGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGCATGCAGAAATTCAGCGGATCTTCGATGATATGATCGTGACg                                                                                                   <  2:4654657/136‑1 (MQ=255)

                                    aCTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACg                                                                                                   <  2:1271317/140‑1 (MQ=255)

                                      tCCTGAACACCTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGCCGATGATATGTTCGAGACGAt                                                                                                 <  2:4060560/140‑1 (MQ=255)

                                      tCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGAt                                                                                                 <  1:4938169/140‑1 (MQ=255)

                                      tCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGAt                                                                                                 <  1:5103437/140‑1 (MQ=255)

                                          gAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTAc                                                                                             >  2:2906943/1‑140 (MQ=255)

                                          gAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTAc                                                                                             <  2:475131/140‑1 (MQ=255)

                                           aaCACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACg                                                                                            <  1:1113013/140‑1 (MQ=255)

                                           aaCACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACg                                                                                            <  2:201747/140‑1 (MQ=255)

                                           aaCACATCTCTGGAGATTGATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTGGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGGGACGATGTACg                                                                                            <  2:3637768/140‑1 (MQ=255)

                                             cacaTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCa                                                                                          <  1:4017175/140‑1 (MQ=255)

                                                aTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaa                                                                                       >  1:3685221/1‑140 (MQ=255)

                                                aTCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaa                                                                                       >  1:2836281/1‑140 (MQ=255)

                                                aTCTCTGGAGATTTATGTCAGGTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaa                                                                                       >  2:447981/1‑140 (MQ=255)

                                                aTCTCCGGCGATTAATTTCAGTCATGCCAGTGTTACACACTCGGAACGAGCGACTTCGCAAAGTTGCTATACCGGAAGGAGAAGTGAAGGCAGAAATTCACCGTATCGTCGATGATATGTTCGAGGCGCAGTACGCagaa                                                                                       >  2:3165840/1‑140 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     >  1:4870292/1‑140 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     <  1:1302665/140‑1 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     <  2:3477995/140‑1 (MQ=255)

                                                  ctctGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCCGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCagaaga                                                                                     <  1:3562871/140‑1 (MQ=255)

                                                     tGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAgg                                                                                  >  2:1465523/1‑140 (MQ=255)

                                                     tGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAgg                                                                                  >  1:1218885/1‑140 (MQ=255)

                                                       gagaTTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGCTATGTTCGAGACGATGTACGCAGAAGAAGGTa                                                                                <  2:4098386/140‑1 (MQ=255)

                                                       gagaTTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTa                                                                                >  2:1135549/1‑140 (MQ=255)

                                                        agaTTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTAt                                                                               <  1:2113939/140‑1 (MQ=255)

                                                          aTTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTg                                                                             >  2:491007/1‑140 (MQ=255)

                                                           tttATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTgg                                                                            >  1:3037661/1‑140 (MQ=255)

                                                            ttATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGc                                                                           >  1:397153/1‑140 (MQ=255)

                                                             tATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGcc                                                                          <  1:502317/140‑1 (MQ=255)

                                                              aTGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCt                                                                         >  1:3639075/1‑140 (MQ=255)

                                                                  cAGTTTTGCAAGTGTTACATATTCCGGACGCGCGGCTTCGCAAAGTTGCCAAACCGGTAGCAGAAGTGAATGCAGAAATCCAGCGTATCGTCGATGATATGCTCGCGACGATGTACGCAGAAGAAGGTATTGGCCTggcg                                                                     <  2:4307031/140‑1 (MQ=255)

                                                                  cAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcg                                                                     <  1:4103075/140‑1 (MQ=255)

                                                                   aGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcgg                                                                    <  1:1030846/140‑1 (MQ=255)

                                                                   aGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTggcgg                                                                    <  1:4612144/140‑1 (MQ=255)

                                                                        tGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAcc                                                               <  2:3853913/140‑1 (MQ=255)

                                                                        tGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAcc                                                               <  2:4804615/140‑1 (MQ=255)

                                                                        tGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAcc                                                               <  2:2905483/140‑1 (MQ=255)

                                                                        tGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAcc                                                               <  2:305427/140‑1 (MQ=255)

                                                                         gCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAAccc                                                              <  2:1656365/140‑1 (MQ=255)

                                                                             gtgtTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGt                                                          <  2:2093037/140‑1 (MQ=255)

                                                                               gtTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTg                                                        >  2:3258753/1‑140 (MQ=255)

                                                                                ttACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTACGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGa                                                       <  1:3072307/140‑1 (MQ=255)

                                                                                 tACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGat                                                      <  2:2189690/140‑1 (MQ=255)

                                                                                 gaCATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCTGCAACCCAGGTTGat                                                      <  1:2779327/139‑1 (MQ=255)

                                                                                        tCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATc                                               >  1:2041187/1‑140 (MQ=255)

                                                                                         ccGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCa                                              <  2:671665/140‑1 (MQ=255)

                                                                                         ccGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCa                                              <  1:1078180/140‑1 (MQ=255)

                                                                                         ccGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCa                                              <  1:871445/140‑1 (MQ=255)

                                                                                          cgaaCGAGCGCCTTCGCAAAGTAGCGAAAACGGTCGAAGAAGTGAATGCAGAAAATCAGAGTATCGTCGATGATATGTTCGCGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCaa                                             <  2:1889832/137‑1 (MQ=255)

                                                                                          cGGACGAGCGGCTTCGCAAAGTTGCTAGACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATTATATGTTCGAGACGATGTACTCAGAAGAATGGATTGTCCTGGCGGCAACCCAGGTTGATGTCCATCaa                                             >  2:3900454/1‑140 (MQ=255)

                                                                                          cGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCTAGGTTGATATCCATCaa                                             >  2:754611/1‑140 (MQ=255)

                                                                                          cGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGGAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTAGTGGCCTGGCGGCAACCCATGGTGATATCCATCaa                                             >  1:1421315/1‑140 (MQ=255)

                                                                                            gACGAGCTGCTTCGCAAAGTTGCTAAAACGGTAGGAGCAGGGAATGCAGAAATTCAGAGTATCGGCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATACAGCaagg                                           >  2:2319610/1‑138 (MQ=255)

                                                                                            gACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACg                                           >  1:4337491/1‑140 (MQ=255)

                                                                                             aCGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGt                                          >  1:2152507/1‑140 (MQ=255)

                                                                                                 gCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCTAGACGATGTACGCAGAAGACGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCa                                      >  2:3112992/1‑140 (MQ=255)

                                                                                                  cGGCTTCGCAAAGTTGCTAACCCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCAt                                     <  2:3550709/140‑1 (MQ=255)

                                                                                                  cGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCAt                                     >  1:4388785/1‑140 (MQ=255)

                                                                                                          cAAAGTTGCTAAACCGGTAGGAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTg                             >  1:1380938/1‑140 (MQ=255)

                                                                                                             aGTTGCTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATg                          <  1:3812945/140‑1 (MQ=255)

                                                                                                                  cTAAACCGGTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCg                     >  1:2658193/1‑140 (MQ=255)

                                                                                                                         ggTAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAAcc              >  1:3527596/1‑140 (MQ=255)

                                                                                                                           tAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGt            >  1:4783285/1‑140 (MQ=255)

                                                                                                                           tAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGt            <  2:2367372/140‑1 (MQ=255)

                                                                                                                           tAGAAGAAGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGt            <  2:4889591/140‑1 (MQ=255)

                                                                                                                             gaagaaGTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGa          <  2:3423616/140‑1 (MQ=255)

                                                                                                                                gaagTGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGa       <  1:2335555/140‑1 (MQ=255)

                                                                                                                                 tacagAATGCATAAATTCAGCGTGTCGTCTATGATATGTTCGAGACGATTTACGCATAAGATGGTTTTGTCCTGGCGGCAACCCAGGTTGATGTCCATCAACGTATCATTGTTATTGATGTTTCTGAAAACCGTGACGaa      <  2:645175/136‑1 (MQ=255)

                                                                                                                                    tGAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACgg   <  1:2997115/140‑1 (MQ=255)

                                                                                                                                     gAATTCAGAAATTCAGCGTATCCTCAATGTTATTTTTGAAACATTGTACGCCGAAGAAGGTTTTGGCCTGGCGGCAGCCCAGGTTGATTGCCATAAACGTATCATTGTTATTGATCTTTCGGAAAAGCGTGACGAACGGc  <  2:2941582/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTCTCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  1:3380719/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  1:2941690/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  1:3150558/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGc  <  2:566329/140‑1 (MQ=255)

                                                                                                                                     gAATGCAGAAATTCAGAGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGCTGTTTCGGAAAACCGTGACGAACGGc  <  1:2484732/140‑1 (MQ=255)

GGGGATTTGTCTAGAATAGAAGAAATAATCTTTCTAACTCCTGAACACATCTCTGGAGATTTATGTCAGTTTTGCAAGTGTTACATATTCCGGACGAGCGGCTTCGCAAAGTTGCTAAACCGGTAGAAGAAGTGAATACAGAAATTCAGCGTATCGTCGATGATATGTTCGAGACGATGTACGCAGAAGAAGGTATTGGCCTGGCGGCAACCCAGGTTGATATCCATCAACGTATCATTGTTATTGATGTTTCGGAAAACCGTGACGAACGGC  >  NZ_CP025268/3434314‑3434586

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`