Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,516,790 C→T K401K (AAG→AAA)  damX ← cell division protein DamX

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,516,7900CT98.6% 1054.6 / ‑5.5 296K401K (AAG→AAA) damXcell division protein DamX
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base T (153/138);  total (155/141)
Fisher's exact test for biased strand distribution p-value = 3.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

AGCGCTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTAG  >  NZ_CP025268/3516654‑3516926
                                                                                                                                        |                                                                                                                                        
aGCGCTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCtttt                                                                                                                                       >  2:2607546/1‑140 (MQ=255)
     ttCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGGTTTTGGCCTGGACATCTGCTGGCACTGTAGATACCGCTTTTTTCGc                                                                                                                                  >  2:2445396/1‑140 (MQ=255)
      tCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGcc                                                                                                                                 >  1:3579645/1‑140 (MQ=255)
       cAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGCTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGAAATGTAGATTGCGCTTTTTTCGCct                                                                                                                                >  2:1511505/1‑140 (MQ=255)
       cAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCct                                                                                                                                >  2:3413558/1‑140 (MQ=255)
       cAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCct                                                                                                                                >  1:3278232/1‑140 (MQ=255)
        aGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCctc                                                                                                                               <  1:1899710/140‑1 (MQ=255)
              cTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCttt                                                                                                                         >  1:1869183/1‑140 (MQ=255)
               tCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTc                                                                                                                        >  2:2988491/1‑140 (MQ=255)
               tCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTc                                                                                                                        >  2:1457951/1‑140 (MQ=255)
                cccGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCg                                                                                                                       <  1:585089/140‑1 (MQ=255)
                  cGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGaa                                                                                                                     <  1:2718682/140‑1 (MQ=255)
                    cAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGc                                                                                                                   >  2:3188739/1‑140 (MQ=255)
                      aCGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGt                                                                                                                 >  2:4919766/1‑140 (MQ=255)
                       cGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTa                                                                                                                <  1:3529597/140‑1 (MQ=255)
                       cGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTa                                                                                                                <  1:3043875/140‑1 (MQ=255)
                         aTAAACCCTTGCGGGAGATAACCTTGATGACTTCACATCGTCCTGTACCTGACCCAGCGGTTTCGCCCCCGCGTTTTTGGCCTGGACATCTGCTGTCCGTGTACTTACGCCCTTTTTCGGCTCCTCACTCGACGCGTaca                                                                                                              >  2:386983/1‑140 (MQ=255)
                         aTAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCGACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCGCTTCTTTCGCAGCGTaca                                                                                                              >  1:1454848/1‑140 (MQ=255)
                         aTAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTacg                                                                                                              >  1:4491857/1‑139 (MQ=255)
                         aTAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTaca                                                                                                              <  1:2701589/140‑1 (MQ=255)
                         aTAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTaca                                                                                                              >  1:4402935/1‑140 (MQ=255)
                         aTAAACCATTGCCGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTCGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTaca                                                                                                              <  1:4354077/140‑1 (MQ=255)
                              ccATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  2:1994417/140‑1 (MQ=255)
                              ccATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  1:3111949/140‑1 (MQ=255)
                              ccATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTCCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  2:3286218/140‑1 (MQ=255)
                              ccATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGCTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  2:175163/140‑1 (MQ=255)
                              ccAGTGCGGCCGATAAACTTGATTACTCCAGATCGGCCTGTACCTGACGCAGCGGTGTCGACCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGCAGATCCCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCa                                                                                                         <  2:2917742/140‑1 (MQ=255)
                                aTTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGa                                                                                                       <  1:4280786/140‑1 (MQ=255)
                                 ttGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGaa                                                                                                      >  2:1141839/1‑140 (MQ=255)
                                  tGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGaaa                                                                                                     >  2:2711832/1‑140 (MQ=255)
                                   gCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAc                                                                                                    <  2:1629748/140‑1 (MQ=255)
                                   gCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAc                                                                                                    >  1:4135499/1‑140 (MQ=255)
                                    cGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAcc                                                                                                   <  2:55762/140‑1 (MQ=255)
                                          aTAACCTTGATTACTTCATATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACAAGCCACAAACCAGGACa                                                                                             >  2:4976898/1‑140 (MQ=255)
                                            aaCCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGTCCTGGACATCTGCTGGCAATGTAGATCCCGCTTTTTTCGCCCTTTCTTTCGACGCGTACACGCCAGAAACCAGGACATa                                                                                           >  2:1044101/1‑140 (MQ=255)
                                              ccTTGCTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATGCCG‑CTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCa                                                                                        <  2:95672/140‑1 (MQ=255)
                                              ccTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATAcc                                                                                         >  2:3275927/1‑140 (MQ=255)
                                                tttaTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGTCAATGTAGATATCGCTTTTTTCGCCTCTTCTTTCGACGCTGACACGCCAGAAACCAGGACATACCAc                                                                                       >  2:639036/4‑140 (MQ=255)
                                                 tGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACg                                                                                      >  1:2972957/1‑140 (MQ=255)
                                                   aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGc                                                                                    >  2:308852/1‑140 (MQ=255)
                                                   aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGc                                                                                    >  1:4410534/1‑140 (MQ=255)
                                                   aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGc                                                                                    >  1:1766567/1‑140 (MQ=255)
                                                   aTTACTTCAGATCGGCCTGTACCTGACGCAGCGGCTTCGCCCACGGGTTTTTGTCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGCCATACCACGGc                                                                                    >  2:3165/1‑140 (MQ=255)
                                                    ttACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCt                                                                                   <  1:981223/140‑1 (MQ=255)
                                                    ttACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCt                                                                                   <  2:2176871/140‑1 (MQ=255)
                                                        ttCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGAcc                                                                               >  2:3367154/1‑140 (MQ=255)
                                                             aTCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACTGCTGACCATTAc                                                                          >  1:960870/1‑140 (MQ=255)
                                                              tCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcg                                                                         <  1:2901873/140‑1 (MQ=255)
                                                               cGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTAcgc                                                                        >  1:2241494/1‑140 (MQ=255)
                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  1:2087936/140‑1 (MQ=255)
                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  1:872058/140‑1 (MQ=255)
                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  1:806918/140‑1 (MQ=255)
                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     >  1:2320655/1‑140 (MQ=255)
                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  1:281061/140‑1 (MQ=255)
                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGCCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAACCCAGGACATACCACGGCTGACCATTACGcgtc                                                                     <  1:4562394/140‑1 (MQ=255)
                                                                  ccTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACAGCTGCTGGCAATGTAGATACCGCTTGTTTCGCCTCGTCTGTCGAAGCGTACACGCCAGAAACCATGACATGCCACGGCTGACCATTACGcgtc                                                                     >  2:4007100/1‑140 (MQ=255)
                                                                       aCCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTc                                                                <  2:4458506/140‑1 (MQ=255)
                                                                          tGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTGTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATa                                                             <  2:4076504/140‑1 (MQ=255)
                                                                           gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAg                                                            >  1:3164093/1‑140 (MQ=255)
                                                                           gACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAg                                                            >  1:3452973/1‑140 (MQ=255)
                                                                            aCGCATCGGTTTCGCCCCCGGGTTTTTCCCCTGGACATCTGCTGGCAACATCGCTACCGCTCTTTTCCCCTCTTCTTTCAGAGCGTACCCGCCCCAAAACAGGACACCCCGCACCCGCCCATTACGCCTCGttgccacga                                                           >  2:5099412/1‑133 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTTCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAAGCAGGACATACCACGGCTGACCATTACGCGTGGTTTCATAGa                                                           >  1:1126768/1‑140 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           >  1:1949750/1‑140 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           >  1:700598/1‑140 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           <  1:68130/140‑1 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           >  1:2022260/1‑140 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           >  1:121753/1‑140 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           >  1:4935189/1‑140 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGa                                                           <  1:3681936/140‑1 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACCCGTCGTTTCATAGa                                                           >  2:2500227/1‑140 (MQ=255)
                                                                            aCGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCACGACATACCACGGCTGACCACTACGCGTCGTTTCAtcgc                                                           >  2:1717351/1‑137 (MQ=255)
                                                                                aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAAc                                                       >  2:462878/1‑140 (MQ=255)
                                                                                aGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCAGAGACAAc                                                       >  1:477518/1‑140 (MQ=255)
                                                                                  cGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATCCCACGGCTGACCATTACGCGTCGTTTCATAGACAACGt                                                     <  1:1665023/140‑1 (MQ=255)
                                                                                  cGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGt                                                     >  1:3889586/1‑140 (MQ=255)
                                                                                    gTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAg                                                   <  2:350223/140‑1 (MQ=255)
                                                                                       tCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGttt                                                >  2:2891139/1‑140 (MQ=255)
                                                                                              cGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGAt                                         >  1:1539127/1‑140 (MQ=255)
                                                                                               gggTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGAtt                                        >  1:3327549/1‑140 (MQ=255)
                                                                                                     ttGGCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGTGCACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCtcttt                                  <  1:4221667/140‑1 (MQ=255)
                                                                                                       ggCCTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCtctttct                                <  2:4718412/140‑1 (MQ=255)
                                                                                                         ccTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTGGGCTCTTCTTTCGAAGCGTAGACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCATATCATAGACAACGTAGTTTTTGAGATTCGCGtttttc                              >  2:2165622/1‑140 (MQ=255)
                                                                                                         ccTGGACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTttcttc                              >  1:4050501/1‑140 (MQ=255)
                                                                                                           tGGACATCTGCTGGCAAGGTAGATACCGCTTTTTTCGCCGCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGc                            >  1:1592058/1‑140 (MQ=255)
                                                                                                            ggACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGcc                           >  1:2586085/1‑140 (MQ=255)
                                                                                                            ggACATCTGCTGGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGcc                           <  1:1697531/140‑1 (MQ=255)
                                                                                                               cATCTGCTGGGAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCaa                        <  2:70243/140‑1 (MQ=255)
                                                                                                                   tgctgGCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCTAACCGt                    >  2:3597123/1‑140 (MQ=255)
                                                                                                                       ggCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAg                >  1:4009101/1‑140 (MQ=255)
                                                                                                                       ggCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAg                >  1:4848713/1‑140 (MQ=255)
                                                                                                                       ggCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACAAGCCAGAAACCAGCACATACCACGGCTGACCATTACGCGTCGGTTCATAGACAACGGAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAg                >  2:1210258/1‑140 (MQ=255)
                                                                                                                       ggCAATCTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTCTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAg                >  2:2569231/1‑140 (MQ=255)
                                                                                                                        gccaTGTATATACCGCTTTTTTCGCCTCTTCTCTCGAAGCGTACACGCCAGTAACCAGGACATACCACGGCTGACCATTACGTGTCGTTTCCTAGACAACGTAGTTTTTCAGCTTCTCTTTCTTCGCCCAACCCTTCAgg               <  2:3746762/137‑1 (MQ=255)
                                                                                                                        gCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAgg               <  2:460429/140‑1 (MQ=255)
                                                                                                                        gCAATGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAgg               <  1:627295/140‑1 (MQ=255)
                                                                                                                          aaTGTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGtt             >  1:4267177/1‑140 (MQ=255)
                                                                                                                             gTAGATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACACCGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGCTTGTc          <  2:41783/140‑1 (MQ=255)
                                                                                                                                gATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTa       <  1:1276507/140‑1 (MQ=255)
                                                                                                                                gATACCGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTa       <  1:1125054/140‑1 (MQ=255)
                                                                                                                                     cGCTTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTag  <  2:753188/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
AGCGCTTCAGGCAACTCCCGCAACGATAAACCATTGCGGGAGATAACCTTGATTACTTCAGATCGGCCTGTACCTGACGCAGCGGTTTCGCCCACGGGTTTTTGGCCTGGACATCTGCTGGCAATGTAGATACCGCCTTTTTCGCCTCTTCTTTCGAAGCGTACACGCCAGAAACCAGGACATACCACGGCTGACCATTACGCGTCGTTTCATAGACAACGTAGTTTTTCAGATTCTCTTTCTTCGCCCAACCGTTCAGGTTGTCGTAGTTAG  >  NZ_CP025268/3516654‑3516926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑