BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,567,287	T→C	478 (TAA→TAG)	glgA ←	glycogen synthase GlgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,567,287	0	T	C	99.7%	1093.1 / NA	330	478 (TAA→TAG)	glgA	glycogen synthase GlgA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (163/166); minor base G (1/0); total (164/166)
Fisher's exact test for biased strand distribution p-value = 4.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 330 total aligned reads displayed.

TTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAATTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATAGCC  >  NZ_CP025268/3567155‑3567422

  tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                                                                                                                <  1:1234708/140‑1 (MQ=255)

   aTTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGAGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGc                                                                                                                               <  1:1358641/140‑1 (MQ=255)

     gggCGACGACCGGGTCCTTTCCACTCGTAAACAGCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACACTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGa                                                                                                                             <  2:976328/139‑1 (MQ=255)

          acgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTa                                                                                                                        >  2:548255/1‑140 (MQ=255)

          acgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTa                                                                                                                        <  2:1641488/140‑1 (MQ=255)

          acgacCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGAGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGATTATGTAAACGGAGCATTCATATAGGCGCTTCCTGAAAACTATTTCGATCGATCGTa                                                                                                                        >  2:3739820/1‑140 (MQ=255)

             acCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAg                                                                                                                     >  2:1476719/1‑140 (MQ=255)

               cGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCt                                                                                                                   <  1:108139/140‑1 (MQ=255)

               cGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCt                                                                                                                   <  1:1289544/140‑1 (MQ=255)

                 ggTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCa                                                                                                                 <  1:4606346/140‑1 (MQ=255)

                 ggTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCa                                                                                                                 <  2:3613135/140‑1 (MQ=255)

                  gTCCTTTCCACTCGTAAACATCAGCTTGTAAGCGATACAGTACTTAAGAGCTTCTACCATAAGCGTCGGCGATGAATATGTAGCCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCAc                                                                                                                <  2:3143897/140‑1 (MQ=255)

                               gTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGAACGACTTcgc                                                                                                   >  2:4347069/1‑140 (MQ=255)

                                tAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGAc                                                                                                        >  1:990446/1‑134 (MQ=255)

                                  aaCATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgccgc                                                                                                >  1:3438940/1‑140 (MQ=255)

                                     aTCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGAc                                                                                             <  2:3428437/140‑1 (MQ=255)

                                         ggttGTCAGCGAGGGAGTGATTGAGAGCTTTGACGCTAAGGGTAGGCGATGAAAATGTAAGCGGAGGATTCATATAGGCGTTTCCTGAAAACTATTTCAGGCGGTATGAACGCTCACGGTACGTTTCCGCCGCGACCTGc                                                                                         <  2:4861124/138‑1 (MQ=255)

                                         gCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGc                                                                                         >  1:4082074/1‑140 (MQ=255)

                                              tAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCt                                                                                    >  2:2211223/1‑140 (MQ=255)

                                               aaGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTa                                                                                   >  1:1041968/1‑140 (MQ=255)

                                                    aTAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTTATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATc                                                                              >  2:501019/1‑140 (MQ=255)

                                                    aTAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATc                                                                              >  1:5025836/1‑140 (MQ=255)

                                                    aTAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATc                                                                              >  2:1080920/1‑140 (MQ=255)

                                                      agagTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCa                                                                            <  1:2016085/140‑1 (MQ=255)

                                                        agTGCTTAAGCGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGTAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGCTAGTATAGCTCACGGTACGACTTCGCCTCGACCTGCCAGCTAAAATCCAtt                                                                          <  2:65702/140‑1 (MQ=255)

                                                        agTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCAtt                                                                          >  1:884208/1‑140 (MQ=255)

                                                            ctcaaGAGCTTCTACGCGAAGAGTGGGCGATGAATCTGCACCCGGAGCATGAATATACGCGTTTCATGAAAACTATTTCAAGCGATAGTAAAGCTCCCGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGcta                                                                      >  2:3491875/4‑138 (MQ=255)

                                                            cTTAAGAGCTTCTACGCTCAGCGTGAGCGATGAATATGTAAACGGAGCATTCATATAGGCGCTTCCTGAAAACTATTTCAAGCGAAAGTAAATCTCACTTTACGACTTCGCCGCGACCGGCCAGCTAATATCCATTCCCa                                                                      >  2:4451376/1‑140 (MQ=255)

                                                            cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                      >  2:5055313/1‑140 (MQ=255)

                                                            cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                      >  1:4463003/1‑140 (MQ=255)

                                                            cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCAACCTGCCAGCTAAAATCCATTGCCa                                                                      >  2:4605860/1‑140 (MQ=255)

                                                                 gagCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGcc                                                                 <  1:1944321/140‑1 (MQ=255)

                                                                 gagCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGcc                                                                 <  2:1234191/140‑1 (MQ=255)

                                                                 gagCTTCTACGATAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGcc                                                                 <  2:1563521/140‑1 (MQ=255)

                                                                    attCTACGCTAAGCGTGGGCGTTGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGTTAGTAAAGCTCACGGTACGACTACGCCGCGGCCTGCCAGCTAAAATACGTTGCCAGAGCCTGa                                                              >  2:4228913/2‑140 (MQ=255)

                                                                      tctcCGCTGAGCGTGGGCTATGTGTATCTAAAGGGTGCGTTCACATACGCGTTTCCGGTGAACCATTTCACTCTCTAGTAAAGCCCACGGTACGACTTCGCCGCCACCTGCAAGCTAAAATCCATTGCCATAGCCTGACg                                                            <  2:3173881/140‑1 (MQ=255)

                                                                      tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCT‑AAATCCATTGCCATAGCCTGACGt                                                           >  1:1416310/1‑140 (MQ=255)

                                                                       cTACGCGAAGCGTGGGCGATGAATATGTAAACGGAGCGTTCATAAAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTTACGATACGACTTCGCAGCGACCGGCCAGCTAAAATCCATTCCCATAGCCTGACGt                                                           >  2:210644/1‑140 (MQ=255)

                                                                         aCGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTGTCCTGAAAACTATTTCAAGCGATATTAAAGCTCACGGTTCGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCGGAGGTTg                                                         >  2:3872324/1‑140 (MQ=255)

                                                                              aaGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACaa                                                    >  1:4988682/1‑140 (MQ=255)

                                                                              aaGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACaa                                                    >  1:4314260/1‑140 (MQ=255)

                                                                               aGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACaaa                                                   >  1:1830487/1‑140 (MQ=255)

                                                                               aGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACaaa                                                   >  1:2581005/1‑140 (MQ=255)

                                                                               aGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACaaa                                                   >  2:3038939/1‑140 (MQ=255)

                                                                                 cGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAAcc                                                 >  2:1291569/1‑140 (MQ=255)

                                                                                  gTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCg                                                <  2:3942126/140‑1 (MQ=255)

                                                                                    gggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGCTAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAACTCCATTGCCATAGCCTGACGTTGCACAAACCGcc                                              >  1:4981843/1‑140 (MQ=255)

                                                                                     ggCTATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCAGAGCCTGACGTTGCACAAACCGCca                                             <  2:2370826/140‑1 (MQ=255)

                                                                                     ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAACGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             <  1:1926288/140‑1 (MQ=255)

                                                                                     ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGGCGTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             >  1:4220136/1‑140 (MQ=255)

                                                                                     ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             >  1:707139/1‑140 (MQ=255)

                                                                                     ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             <  1:4035849/140‑1 (MQ=255)

                                                                                     ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             <  2:478501/140‑1 (MQ=255)

                                                                                     ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             >  2:2302851/1‑140 (MQ=255)

                                                                                     ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCACTGCCATAGCCTGACGTTGCACAAACCGCca                                             <  2:4666674/140‑1 (MQ=255)

                                                                                      gCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCcac                                            >  2:2882857/1‑140 (MQ=255)

                                                                                       cGATGCATATGTAAACGGAGCATTCATATCGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCcaca                                           <  1:559465/140‑1 (MQ=255)

                                                                                          tGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTg                                        <  1:1731887/140‑1 (MQ=255)

                                                                                          tGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACCATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGAGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTg                                        <  2:4171042/140‑1 (MQ=255)

                                                                                             atatGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAg                                     <  1:3282356/140‑1 (MQ=255)

                                                                                               atGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGa                                   <  1:3748242/140‑1 (MQ=255)

                                                                                                tGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGAc                                  >  1:1274244/1‑140 (MQ=255)

                                                                                                  tAAACGGAGCATTCATATAGGCGATTCCTGAAAACTATTTCAAGCGATAGTTAAGCTCACGGTACGACTTCGACGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACgg                                >  1:3059745/1‑140 (MQ=255)

                                                                                                    aaCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGa                              >  1:4756461/1‑140 (MQ=255)

                                                                                                     aCGGAGCCTTCATATAGGCGTTTTCTGAAAACTCTTTCAAGCGATAGTAAAGTGCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                             <  2:1366798/140‑1 (MQ=255)

                                                                                                     aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                             <  1:4883570/140‑1 (MQ=255)

                                                                                                     aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                             <  1:2198743/140‑1 (MQ=255)

                                                                                                     aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                             <  2:3203185/140‑1 (MQ=255)

                                                                                                      cGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAcc                            >  1:4667132/1‑140 (MQ=255)

                                                                                                       ggAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACca                           <  2:2596125/140‑1 (MQ=255)

                                                                                                       ggAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACca                           <  1:1760734/140‑1 (MQ=255)

                                                                                                       ggAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACca                           <  2:266913/140‑1 (MQ=255)

                                                                                                       ggAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACca                           <  1:1536482/140‑1 (MQ=255)

                                                                                                       ggAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACca                           <  2:1805087/140‑1 (MQ=255)

                                                                                                       ggAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACca                           <  1:3583672/140‑1 (MQ=255)

                                                                                                       ggAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACca                           <  1:369000/140‑1 (MQ=255)

                                                                                                       agAGCATTCATATGTGCGTTTCCTGAACACTATTGCAAGCGATAGTAAAGCTCACGGTACGCCTTCGCCGCGACGTGCCAGCGAACATCCATTGCCATAGCCAGAATTCGCACAAACCGCCACAGGGAAGGACGGGAAca                           <  2:736079/139‑1 (MQ=255)

                                                                                                        gAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcac                          >  1:1200855/1‑140 (MQ=255)

                                                                                                        gAGCATTCATATAGGCGTTGCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcac                          <  2:198862/140‑1 (MQ=255)

                                                                                                         aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                         >  1:2788019/1‑140 (MQ=255)

                                                                                                          gCATTCATATAGGCGTTTCCTGAAATCTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAACATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAg                        <  2:4293180/140‑1 (MQ=255)

                                                                                                          gCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAg                        >  2:217203/1‑140 (MQ=255)

                                                                                                          gCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAg                        >  1:939223/1‑140 (MQ=255)

                                                                                                           cATTCATATAGGCGTGGCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAAGCCATTGCCAGAGCCTGACGTTGCACAAACCGCCACATGTAAGGACGGTACCACAGt                       >  1:3871085/1‑140 (MQ=255)

                                                                                                           cATGCATATATGCGTTGCCTGAACACTATTTCAAGCGGTATTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTTCCATAGCCTGACGTTGCCCAAACCGCCCCAGTGAAGGACGCGACCCCAGt                       >  2:3255176/1‑140 (MQ=255)

                                                                                                              tCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACa                    <  1:1519867/140‑1 (MQ=255)

                                                                                                                 tataGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACaaaa                 <  1:2304026/140‑1 (MQ=255)

                                                                                                                 cataCGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCCCACTGAAGGACGGGACCACAGTACaaaa                 >  2:4727955/2‑140 (MQ=255)

                                                                                                                   taGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGTCGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGc               <  2:4049156/140‑1 (MQ=255)

                                                                                                                   taGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGc               <  1:1777772/140‑1 (MQ=255)

                                                                                                                      gCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCAAAAACCGCCACAGTGAAGGCCGGGACCACAGTACAAAAGCAcc            <  2:4942674/140‑2 (MQ=255)

                                                                                                                         tttCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCg         >  2:2585560/1‑140 (MQ=255)

                                                                                                                           tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCCCCAACCGCCACAGTGAAGGCCGGGACCACAGTACAAAAGCACGTCGaa       >  2:1721949/1‑140 (MQ=255)

                                                                                                                           tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       >  2:3776263/1‑140 (MQ=255)

                                                                                                                           tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       >  2:2764387/1‑140 (MQ=255)

                                                                                                                           tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  2:2077397/140‑1 (MQ=255)

                                                                                                                           tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  2:1874050/140‑1 (MQ=255)

                                                                                                                           tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  1:2968236/140‑1 (MQ=255)

                                                                                                                           tCCTCAAAACTATTTCAAGCGGTCGTACAGCTCACGGTACGACTTCGCCGCGACCTGCCACCTAAAATCCATGGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  2:1770269/140‑1 (MQ=255)

                                                                                                                             cTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATa     <  2:3987768/140‑1 (MQ=255)

TTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAATTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATAGCC  >  NZ_CP025268/3567155‑3567422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`