BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,567,938	T→C	L261L (TTA→TTG)	glgA ←	glycogen synthase GlgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,567,938	0	T	C	99.2%	807.9 / ‑4.5	250	L261L (TTA→TTG)	glgA	glycogen synthase GlgA
Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (108/139); total (109/141)
Fisher's exact test for biased strand distribution p-value = 5.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 250 total aligned reads displayed.

AGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCGA  >  NZ_CP025268/3567801‑3568073

agaCCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTAAACCTTAAGCCCCATTGCGATTTGTAACGGGCGCTTATTTTCGGCTTTATCTTCCAACGTAGAGCGGGCGTATCGCGAGGCCaa                                                                                                                                       >  2:356186/1‑140 (MQ=255)

    cTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGt                                                                                                                                   <  1:1129735/140‑1 (MQ=255)

    cTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGt                                                                                                                                   <  1:925494/140‑1 (MQ=255)

       tCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAg                                                                                                                                >  2:2506860/1‑140 (MQ=255)

         tggctggTCAGACGGCTCACCCCTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                                                                                                              <  1:210644/140‑1 (MQ=255)

         tggctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                                                                                                              <  1:45029/140‑1 (MQ=255)

         tggctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                                                                                                              <  1:2430760/140‑1 (MQ=255)

         tggctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                                                                                                              <  1:2504889/140‑1 (MQ=255)

         tggctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTACCGCGGGTGTAACGCGAGGCCAACAGTAAGTc                                                                                                                              <  2:2579216/140‑1 (MQ=255)

           gctggTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCg                                                                                                                            >  1:645854/1‑140 (MQ=255)

                tCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTctct                                                                                                                       >  1:3915297/1‑140 (MQ=255)

                         tCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTGCAg                                                                                                              >  2:3553631/1‑140 (MQ=255)

                              acTGCAAATGCCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGTCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCTGGTGTAACGCGAGGCCAACACTAAGTCCGTCTCTGGACTCCAGAtgtt                                                                                                         <  2:3739296/140‑1 (MQ=255)

                               cTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTtc                                                                                                        <  2:1275071/140‑1 (MQ=255)

                                  cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGCTTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCg                                                                                                     >  2:1455122/1‑140 (MQ=255)

                                  cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCg                                                                                                     >  2:1053839/1‑140 (MQ=255)

                                  cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCg                                                                                                     >  1:3071870/1‑140 (MQ=255)

                                  cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCg                                                                                                     >  2:1512050/1‑140 (MQ=255)

                                  cAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCg                                                                                                     >  2:3447733/1‑140 (MQ=255)

                                      aaGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCa                                                                                                 <  1:3544146/140‑1 (MQ=255)

                                       aGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCAc                                                                                                >  2:4782026/1‑140 (MQ=255)

                                       aGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCAc                                                                                                >  2:1360856/1‑140 (MQ=255)

                                        gCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                               <  2:1836527/140‑1 (MQ=255)

                                        gCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                               <  1:2026070/140‑1 (MQ=255)

                                        gCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                               <  2:1895343/140‑1 (MQ=255)

                                        gCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                               <  2:3762779/140‑1 (MQ=255)

                                        gCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                               <  2:2469585/140‑1 (MQ=255)

                                        gCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                               <  1:1624697/140‑1 (MQ=255)

                                        gCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACg                                                                                               <  2:4651503/140‑1 (MQ=255)

                                          ggCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGcc                                                                                             >  1:2355321/1‑140 (MQ=255)

                                          ggCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGcc                                                                                             <  1:2663198/140‑1 (MQ=255)

                                                 tATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTCGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGt                                                                                      >  1:2155815/1‑140 (MQ=255)

                                                 tATCCTCAACCTTAAGCCCCCTCGCGATTTGTACATGGCGCTTATTTTCCGCTCTATCTTCCCACGTATCGCGGGTGTAACGCGAGGCCCACAGTCAGTCCGTCTCCGGACTCCAGATTTTCTCGTCCCCGCCGTTCAGt                                                                                      <  2:3028609/140‑1 (MQ=255)

                                                      tCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTTCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGcc                                                                                 >  2:4672026/1‑140 (MQ=255)

                                                      tCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGcc                                                                                 >  2:4854679/1‑140 (MQ=255)

                                                         aCCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGa                                                                              >  2:2598602/1‑140 (MQ=255)

                                                             tAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGa                                                                          >  2:2182988/1‑140 (MQ=255)

                                                             tAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCGTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTCGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGa                                                                          >  2:4493761/1‑140 (MQ=255)

                                                              aaGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGAc                                                                         <  1:1667924/140‑1 (MQ=255)

                                                              aaGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGAc                                                                         >  2:1218985/1‑140 (MQ=255)

                                                              aaGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGAc                                                                         <  2:2064305/140‑1 (MQ=255)

                                                              aaGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGAc                                                                         >  1:4413694/1‑140 (MQ=255)

                                                               aGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACg                                                                        <  1:1018554/140‑1 (MQ=255)

                                                               aGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACg                                                                        <  2:826561/140‑1 (MQ=255)

                                                               aGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACg                                                                        <  1:1910569/140‑1 (MQ=255)

                                                               aGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACg                                                                        <  1:19670/140‑1 (MQ=255)

                                                               aGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACg                                                                        <  1:1512050/140‑1 (MQ=255)

                                                                    cATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGAc                                                                                                                   <  1:35675/92‑1 (MQ=255)

                                                                    cATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCtt                                                                   >  1:2622104/1‑140 (MQ=255)

                                                                    cATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCtt                                                                   >  1:3314435/1‑140 (MQ=255)

                                                                     aTTGCGATTTGTAACTGGCTCTTCTTTTCCGCTGTATCTTCCGACGTCTCGCGGGTGTATCTCGAGGCCCACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTc                                                                  >  2:2924007/1‑140 (MQ=255)

                                                                     aTTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGCCTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCGTTc                                                                  >  2:3179692/1‑140 (MQ=255)

                                                                     aTTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTc                                                                  >  1:3698027/1‑140 (MQ=255)

                                                                     aTTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTc                                                                  >  1:4903520/1‑140 (MQ=255)

                                                                     aTTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTc                                                                  >  1:1233463/1‑140 (MQ=255)

                                                                      ttGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCa                                                                 <  1:719251/140‑1 (MQ=255)

                                                                      ttGCGATTTGTAACTGACGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCa                                                                 <  1:2840157/140‑1 (MQ=255)

                                                                           aTTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTTCAGATTTTCTCGCCCACGCCTTTCAGTACGCCGCAAAGACTCCCTTCACGGTg                                                            >  2:627202/1‑140 (MQ=255)

                                                                           aTTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTg                                                            >  1:4592086/1‑140 (MQ=255)

                                                                            tttCTAACTGGCGCTTGTTTTCCGCTTTAGCCTCCACCGTATCGCGGGTGTAACGCGACGACAACACTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCGCGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGa                                                           <  2:579865/140‑1 (MQ=255)

                                                                             ttGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGAc                                                          <  1:4193513/140‑1 (MQ=255)

                                                                             ttGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGAc                                                          <  2:221728/140‑1 (MQ=255)

                                                                              tGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACg                                                         <  1:845303/140‑1 (MQ=255)

                                                                              tGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACg                                                         >  1:2686610/1‑140 (MQ=255)

                                                                              tGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACg                                                         >  1:2455863/1‑140 (MQ=255)

                                                                               gtcaCTGGCGCTTATTTGCCGCTTTATCTACCGCCGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTCTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGc                                                        <  2:2020687/137‑1 (MQ=255)

                                                                               gTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGc                                                        <  1:1306536/140‑1 (MQ=255)

                                                                               gTAACTGGCGCTTATTTTCCGCTCTCTCTTCCAACGTATCGCGGGTGTAACGCGAGGCCCACTGTATTTCTGTCCCTGGACTACAGATTTTCCCGTCCACGCCGTCCAGTAAGCCGGAAAGACGCCCTTCATGGTGACGc                                                        <  2:435381/140‑1 (MQ=255)

                                                                                 aaCTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTg                                                      <  1:4775412/140‑1 (MQ=255)

                                                                                      gcgcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCa                                                 >  1:1571383/1‑140 (MQ=255)

                                                                                        gcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAAc                                               <  1:4402262/140‑1 (MQ=255)

                                                                                        gcTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAAc                                               >  1:116160/1‑140 (MQ=255)

                                                                                          ttattaTCCGCTTTCTCTTCCACCGTATCGCGGGTGCAACGAGAGGACAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTAAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                             <  2:501143/140‑1 (MQ=255)

                                                                                          ttATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTTACTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                             <  2:1636461/140‑1 (MQ=255)

                                                                                          ttATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                             <  1:3254665/140‑1 (MQ=255)

                                                                                          ttATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                             <  1:938973/140‑1 (MQ=255)

                                                                                          ttATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                             <  1:4345510/140‑1 (MQ=255)

                                                                                          ttATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                             <  2:4151510/140‑1 (MQ=255)

                                                                                            aTTTTCCGCTTTATCTGCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGCCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGAc                                           <  1:4068805/140‑1 (MQ=255)

                                                                                                 ccGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTcc                                      <  1:567391/140‑1 (MQ=255)

                                                                                                 ccGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTcc                                      <  1:1046963/140‑1 (MQ=255)

                                                                                                  cGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCa                                     <  1:1699351/140‑1 (MQ=255)

                                                                                                   gCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCAt                                    <  1:1828577/140‑1 (MQ=255)

                                                                                                             ccAACGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCa                          <  1:4723063/140‑1 (MQ=255)

                                                                                                                 cGTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACt                      >  1:2917186/1‑140 (MQ=255)

                                                                                                                  gTATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTg                     <  1:3168034/140‑1 (MQ=255)

                                                                                                                   tATCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGc                    >  1:2271383/1‑140 (MQ=255)

                                                                                                                     tCGCGGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCgg                  <  1:187681/140‑1 (MQ=255)

                                                                                                                       gcgGGTGTAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGtt                <  2:3342373/140‑1 (MQ=255)

                                                                                                                           gtgtAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGt            <  2:366891/140‑1 (MQ=255)

                                                                                                                            tgtAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTg           >  2:714034/1‑140 (MQ=255)

                                                                                                                            tgtAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTg           >  2:5068450/1‑140 (MQ=255)

                                                                                                                             gtgaCGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCGCGTCCACGCCGTGCAGTGCGCCGGAAAGAAGTCCTTCACGGGGGCGCGGTGGCGGGAGACCTTCCAGACGGGATGGAAATTGCGGGTCgtgga          >  2:2892121/4‑136 (MQ=255)

                                                                                                                              tAACGCGAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGAt         <  2:1626215/140‑1 (MQ=255)

                                                                                                                                  gcgAGGCCAACAGTCAGGTCGTCTCTGGTCTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACTGTGACGCTGTTGCAACAGACCTTCCATACCGTAGTCAAACTGCGGTCCGGTGATCTcg     <  2:3632905/140‑1 (MQ=255)

                                                                                                                                   cgAGGCCACCAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGATGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcgc    >  2:4081045/1‑140 (MQ=255)

                                                                                                                                   cgAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcgc    <  1:4681568/140‑1 (MQ=255)

                                                                                                                                   cgAGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTcgc    <  1:4854679/140‑1 (MQ=255)

                                                                                                                                     aGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAg                                             <  1:5054360/97‑1 (MQ=255)

                                                                                                                                     aGGCCAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCGa  >  1:3688402/1‑140 (MQ=255)

AGACCTTTCTGGCTGGTCAGACGGCTCACCACTGCAAAAAGCGGCACTTTATCGTCAACCTTAAGCCCCATTGCGATTTGTAACTGGCGCTTATTTTCCGCTTTATCTTCCAACGTATCGCGGGTGTAACGCGAGGCTAACAGTAAGTCCGTCTCTGGACTCCAGATTTTCTCGTCCACGCCGTTCAGTACGCCGGAAAGACGCCCTTCACGGTGACGCTGTTGCAACAGACCTTCCATACCGTAGGCAAACTGCGGTTCGGTGATCTCGCGA  >  NZ_CP025268/3567801‑3568073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`