BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,644,586	A→G	G428G (GGT→GGC)	prlC ←	oligopeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,644,586	0	A	G	99.7%	1017.9 / ‑4.8	309	G428G (GGT→GGC)	prlC	oligopeptidase A
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (129/179); total (130/179)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.17e-01

Only 100 of 309 total aligned reads displayed.

CGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACAT  >  NZ_CP025268/3644450‑3644722

cGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCt                                                                                                                                       >  1:3416735/1‑140 (MQ=255)

cGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCt                                                                                                                                       >  2:1462123/1‑140 (MQ=255)

cGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCt                                                                                                                                       <  2:1756722/140‑1 (MQ=255)

 gTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTa                                                                                                                                      <  1:2697063/140‑1 (MQ=255)

 gTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTa                                                                                                                                      <  1:4183330/140‑1 (MQ=255)

  tGACCGCACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGCTTACCATTTCCCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGCTTTTGCAAAGAACCAGCAGCTCTACGCATCTGGCCTAc                                                                                                                                     >  2:2790881/1‑140 (MQ=255)

  tGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTAc                                                                                                                                     >  1:4793013/1‑140 (MQ=255)

   gACCGAGCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    <  2:263339/140‑1 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTCCg                                                                                                                                    >  2:4117164/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    <  2:107159/140‑1 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:1590965/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:1614324/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    >  1:4423252/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACACATCTGGCCTACg                                                                                                                                    >  2:4382992/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGGTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTCACGCATCTGGCCTACg                                                                                                                                    >  1:1079679/1‑140 (MQ=255)

   gACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGCTTAGCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACg                                                                                                                                    <  1:3625953/140‑1 (MQ=255)

     ccGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACCGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCa                                                                                                                                  <  2:4346075/140‑1 (MQ=255)

     ccGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCa                                                                                                                                  <  1:4481259/140‑1 (MQ=255)

     ccGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCa                                                                                                                                  <  2:1297169/140‑1 (MQ=255)

     ccGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCa                                                                                                                                  <  1:838534/140‑1 (MQ=255)

        aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTc                                                                                                                               >  1:1780051/1‑140 (MQ=255)

        aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTc                                                                                                                               >  1:1360193/1‑140 (MQ=255)

        aaCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTGTTGCAAAGAACCATCAGCTTTACGCAGCTGGCCTACGCAGTc                                                                                                                               >  2:3899988/1‑140 (MQ=255)

         aCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTca                                                                                                                              <  1:3159373/140‑1 (MQ=255)

         aCTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTca                                                                                                                              <  1:1128621/140‑1 (MQ=255)

              tGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTcatccat                                                                                                                         >  1:3196601/1‑140 (MQ=255)

              tGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTcatccat                                                                                                                         >  1:2654783/1‑140 (MQ=255)

               ggAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTcatccatc                                                                                                                        >  1:2870125/1‑140 (MQ=255)

               ggAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTcatccatc                                                                                                                        >  2:1312154/1‑140 (MQ=255)

                       gTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGcccc                                                                                                                >  1:1861156/1‑140 (MQ=255)

                           tCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccgccg                                                                                                            >  2:1111794/1‑140 (MQ=255)

                           tCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCccgccg                                                                                                            >  1:1583752/1‑140 (MQ=255)

                              cTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTAAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCgcgc                                                                                                         <  1:4239842/140‑1 (MQ=255)

                                    cgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGttt                                                                                                   >  1:3293518/1‑140 (MQ=255)

                                    cgtGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGttt                                                                                                   >  2:3374039/1‑140 (MQ=255)

                                     gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGACCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  1:2966444/140‑1 (MQ=255)

                                     gtgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGtttt                                                                                                  <  1:684981/140‑1 (MQ=255)

                                      tgagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTc                                                                                                 >  2:2317438/1‑140 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  1:635577/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  1:4827384/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTGGGTTTCa                                                                                                <  2:3988625/140‑1 (MQ=255)

                                       gagtgaACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGCCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCa                                                                                                <  2:4766002/140‑1 (MQ=255)

                                                 cgcCGGTTTGCCATGTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGAGTGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTAATCAATCCACGTCCCGTGGCGCTTGTTTTCACGGGCATACa                                                                                      >  2:2202530/1‑140 (MQ=255)

                                                 cgcCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACa                                                                                      >  2:713130/1‑140 (MQ=255)

                                                    cGGTTTCCCATTTACCGGGCTGTTGAAGTTACAAATCAAATACGCGACCGCTTTTTGCAAAGACCCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCCTCAACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:1697432/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGCAGTTACAAGTCAAATACGCGACCGGTTTTTGCCACGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  2:4605512/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:3015792/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:943959/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:367371/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  2:2998635/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  2:625293/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:217285/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  2:2468459/140‑1 (MQ=255)

                                                    cGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  1:3897194/140‑1 (MQ=255)

                                                    cGGTTTACCATTTAACGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGAt                                                                                   <  2:471322/140‑1 (MQ=255)

                                                      gTTTACCATTTAGCGGGCGGTTGAAGTTAGAAGTCAAATACGCGACCGGTGTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCAGCCGTCCCCGCCCCGGCGCGCTTGTTTTCACGGGCATACAGATCg                                                                                 <  2:5069400/140‑1 (MQ=255)

                                                      gTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCg                                                                                 >  2:2589858/1‑140 (MQ=255)

                                                          aCCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGt                                                                             <  1:590774/140‑1 (MQ=255)

                                                                tACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGc                                                                       >  2:794182/1‑140 (MQ=255)

                                                                tACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGc                                                                       >  1:3785582/1‑140 (MQ=255)

                                                                 aCCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCt                                                                      >  1:309815/1‑140 (MQ=255)

                                                                     ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAGAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  2:4304371/1‑140 (MQ=255)

                                                                     ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  1:1993208/1‑140 (MQ=255)

                                                                     ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcg                                                                  >  2:1894012/1‑140 (MQ=255)

                                                                     ggCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCCCTTGTTTTCCCGGGCATACCGATCGCGGTAGAAGCTACcg                                                                  >  2:3331680/1‑140 (MQ=255)

                                                                     ggCGGTGGAAGTTACAAGTCAAATAGGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATGGGGGCTGCGGAGGCATCCATCCACGCCCCGCAGCGGTTGTTTTCACGGGCATACAGAGCGAGGTAGAAGCTAGcg                                                                  >  2:3940732/1‑140 (MQ=255)

                                                                       cGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                                                <  1:450586/140‑1 (MQ=255)

                                                                       cGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                                                <  1:3365129/140‑1 (MQ=255)

                                                                       cGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACcgcg                                                                <  2:4956786/140‑1 (MQ=255)

                                                                         gTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCa                                                              <  2:1674493/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATGCGCGAACGGTCTGTGCAAAGAACCATAAGCTTTACGCATATGGCCTACGCAGTCAGCCATCCACGCCCCGCCGCGTTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:3257946/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  1:1026964/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:1590965/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:2019387/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  1:2132666/140‑1 (MQ=255)

                                                                                  aCAAGTCAAATACGCGACCGGTCTGTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTCCCGCGCAGTTCGTTAt                                                     <  2:3568195/140‑1 (MQ=255)

                                                                                        cAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGt                                               >  1:4897820/1‑140 (MQ=255)

                                                                                        cAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGt                                               >  1:4895072/1‑140 (MQ=255)

                                                                                            tACGCGACCGGTTTTTGCAAAGCACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATa                                           <  2:568181/140‑1 (MQ=255)

                                                                                            tACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATa                                           <  1:815204/140‑1 (MQ=255)

                                                                                            tACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATa                                           <  1:4295881/140‑1 (MQ=255)

                                                                                            tACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATa                                           <  1:330705/140‑1 (MQ=255)

                                                                                                 gACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCTTCATACAGtt                                      >  1:4381474/1‑140 (MQ=255)

                                                                                                 gACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGtt                                      >  2:2709773/1‑140 (MQ=255)

                                                                                                 gACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGtt                                      >  1:2221004/1‑140 (MQ=255)

                                                                                                       tttttGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaaga                                <  1:1937917/140‑1 (MQ=255)

                                                                                                       tttttGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaaga                                <  2:3691201/140‑1 (MQ=255)

                                                                                                       tttttGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaaga                                <  1:3278128/140‑1 (MQ=255)

                                                                                                       tttttGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaaga                                <  2:2043015/140‑1 (MQ=255)

                                                                                                        ttttGCAAAGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCgaagaa                               <  1:3971587/140‑1 (MQ=255)

                                                                                                              aaaGAACCATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCCCGCCCCGCCGCGCTTGTTTTCCCGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTAc                         <  1:3118129/140‑1 (MQ=255)

                                                                                                                 gAACCATCAGCTTTACGCATCTGGCCTAGGCAGTGATCCATCCACGCCCCGCCGCGCTTGTGTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATc                      >  2:3029047/1‑140 (MQ=255)

                                                                                                                     cATCAGCTTTACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGa                  <  1:2371450/140‑1 (MQ=255)

                                                                                                                            tttACGCATCTGGCCTAGGCAGTCATCCATCCCAGCCCCGCCGCCATTGTTTTCACGGGCATCCAGAGCGAGGTAGAAGCTACCGCGCAGTTCCTTATATTCGTCATACAGATCGAGGAAAAGTACATCCGGTTGCCAGa           >  2:4529851/1‑140 (MQ=255)

                                                                                                                            tttACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTAt                                                     <  2:2976667/98‑1 (MQ=255)

                                                                                                                              tACGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACa         >  1:2324841/1‑140 (MQ=255)

                                                                                                                                cGCATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATc       <  2:2627045/140‑1 (MQ=255)

                                                                                                                                  cATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCaa     <  1:2336518/140‑1 (MQ=255)

                                                                                                                                  cATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCaa     <  1:4079226/140‑1 (MQ=255)

                                                                                                                                  cATCTGGCCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCaa     <  1:4980457/140‑1 (MQ=255)

CGTGACCGAACTCGTGGAAGAGGGTGATCACTTCGTCGTGAGTGAACAGCGCCGGTTTACCATTTACCGGGCGGTTGAAGTTACAAGTCAAATACGCGACCGGTTTTTGCAAAGAACCATCAGCTTTACGCATCTGACCTACGCAGTCATCCATCCACGCCCCGCCGCGCTTGTTTTCACGGGCATACAGATCGAGGTAGAAGCTACCGCGCAGTTCGTTATTTTCGTCATACAGTTCGAAGAAACGTACATCCGGATGCCAGACATCAACAT  >  NZ_CP025268/3644450‑3644722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`