BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,794,748	T→C	286 (TAA→TAG)	yibB ←	protein YibB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,794,748	0	T	C	99.4%	535.0 / NA	160	286 (TAA→TAG)	yibB	protein YibB
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (57/102); total (57/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

AAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAG  >  NZ_CP025268/3794613‑3794883

       gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                                                                              >  1:1042530/1‑140 (MQ=255)

       gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                                                                              >  2:627768/1‑140 (MQ=255)

       gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                                                                              >  1:285070/1‑140 (MQ=255)

       gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                                                                              >  1:1543399/1‑140 (MQ=255)

       gTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGa                                                                                                                              <  1:734845/140‑1 (MQ=255)

             ttttGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGaa                                                                                                                        <  1:893766/140‑1 (MQ=255)

                tGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAAt                                                                                                                     >  2:2535211/1‑140 (MQ=255)

                   cTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATtct                                                                                                                  <  2:3353025/140‑1 (MQ=255)

                     gggCATAATGATGATTGATTTTACTGCATTGGTATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCa                                                                                                                >  2:4543370/1‑140 (MQ=255)

                     gggCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCa                                                                                                                >  1:1833059/1‑140 (MQ=255)

                       gCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAgc                                                                                                              >  1:2868011/1‑140 (MQ=255)

                       gCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAga                                                                                                              >  2:1183628/1‑139 (MQ=255)

                            atgatgATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTg                                                                                                         <  1:4556676/140‑1 (MQ=255)

                            atgatgATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTg                                                                                                         <  1:4557477/140‑1 (MQ=255)

                                tgattgatTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtt                                                                                                     >  1:3562742/1‑140 (MQ=255)

                                 gattgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAttt                                                                                                    >  1:1360224/1‑140 (MQ=255)

                                  attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTCCCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAACATTCTCAGCGCCTGCAtttt                                                                                                   <  1:2727470/140‑1 (MQ=255)

                                  attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtttt                                                                                                   >  2:2064779/1‑140 (MQ=255)

                                  attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtttt                                                                                                   >  2:3214690/1‑140 (MQ=255)

                                  attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtttt                                                                                                   >  2:3380786/1‑140 (MQ=255)

                                  attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtttt                                                                                                   >  1:3996147/1‑140 (MQ=255)

                                  attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtttt                                                                                                   <  2:4044695/140‑1 (MQ=255)

                                  attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtttt                                                                                                   <  2:1168377/140‑1 (MQ=255)

                                  attgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCAtttt                                                                                                   <  1:1400234/140‑1 (MQ=255)

                                  attgattTTAATGCATTGACATAAACAGAAAAAATAATGCCAAAGCATCAGGTTTCCGATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGACAGGAAACTTCTCAGCGCCTGCAtttt                                                                                                   <  2:4123178/140‑1 (MQ=255)

                                    tgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTg                                                                                                 <  2:52638/140‑1 (MQ=255)

                                    tgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTg                                                                                                 <  2:3228355/140‑1 (MQ=255)

                                    tgattTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTg                                                                                                 <  2:4280068/140‑1 (MQ=255)

                                     gagtttACTGGAGTGATATGAAAAGAAAATATGATGCCAAAGCATCAATATTCCTATCTCAGTTGGTATCAAAACGCTACCCTCGAATCGAGGGAACCCACTTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGc                                                                                                <  2:475851/137‑1 (MQ=255)

                                      attTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGcc                                                                                               >  2:1759766/1‑140 (MQ=255)

                                          tACTGCATTGATATAAAAAGAACAAATGCTGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTa                                                                                           <  1:465532/140‑1 (MQ=255)

                                           aCTGCATTGATATCAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaa                                                                                          <  2:4431661/140‑1 (MQ=255)

                                           aCTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTaa                                                                                          <  2:5086798/140‑1 (MQ=255)

                                               cATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtg                                                                                      >  2:4534665/1‑140 (MQ=255)

                                                aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                                                                     <  2:285070/140‑1 (MQ=255)

                                                aTTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAgtgt                                                                                     <  2:2868011/140‑1 (MQ=255)

                                                 ttGATATACAAAGAAATAATGACGCCAAAGCATCTTCTTTCCTATCTCAGTTGGTATCAAAACTCTACCCACTAATGGAGGGAAACCTATTTGCTTTAAAGGAAAATTCTCAGCGCCTGCATGTTTGCCCCTAACTTGtt                                                                                    <  2:1855456/140‑1 (MQ=255)

                                                 ttGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGtt                                                                                    >  1:447828/1‑140 (MQ=255)

                                                 ttGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGtt                                                                                    <  1:4942201/140‑1 (MQ=255)

                                                 ttGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGtt                                                                                    >  1:1105828/1‑140 (MQ=255)

                                                 ttGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGtt                                                                                    <  1:1613538/140‑1 (MQ=255)

                                                 ttGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACCAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCCTTTTTGCCCCTAAAGTGtt                                                                                    <  1:1613902/140‑1 (MQ=255)

                                                     tataAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTc                                                                                >  2:264886/1‑140 (MQ=255)

                                                         aaaaGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGa                                                                            >  1:2275973/1‑140 (MQ=255)

                                                              aaaaaaTGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAagcag                                                                       <  1:1141500/140‑1 (MQ=255)

                                                                      atgCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACaa                                                               >  2:2758880/1‑140 (MQ=255)

                                                                          cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCg                                                           <  1:592749/140‑1 (MQ=255)

                                                                          cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAACAGCGAAACAAATCg                                                           <  1:967053/140‑1 (MQ=255)

                                                                          cAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCCATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCg                                                           <  2:4518417/140‑1 (MQ=255)

                                                                            aaGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTACTGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTCCTCCTGCAGCAGCGAAACAAATCGaa                                                         <  2:496323/140‑1 (MQ=255)

                                                                            aaGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGaa                                                         <  1:2810402/140‑1 (MQ=255)

                                                                                 tcatTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCAtt                                                    <  2:3713486/140‑1 (MQ=255)

                                                                                 tcatTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCAtt                                                    <  2:2853633/140‑1 (MQ=255)

                                                                                 tcatTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCAtt                                                    <  2:2747122/140‑1 (MQ=255)

                                                                                     ttttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTcc                                                <  1:1037160/140‑1 (MQ=255)

                                                                                     ttttCCTATCTCAGTTGGTATCAAAACGCTACCCACGAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTcc                                                <  1:3971299/140‑1 (MQ=255)

                                                                                      tttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCct                                               <  1:2530705/140‑1 (MQ=255)

                                                                                       ttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCctc                                              <  1:3214690/140‑1 (MQ=255)

                                                                                       ttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCctc                                              <  2:1965643/140‑1 (MQ=255)

                                                                                       ttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCctc                                              <  2:2759528/140‑1 (MQ=255)

                                                                                       ttCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCctc                                              <  1:901523/140‑1 (MQ=255)

                                                                                              ctcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAg                                       <  2:4598412/140‑1 (MQ=255)

                                                                                              ctcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAg                                       <  2:789634/140‑1 (MQ=255)

                                                                                              ctcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAg                                       <  1:5009060/140‑1 (MQ=255)

                                                                                              ctcAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAg                                       <  1:538986/140‑1 (MQ=255)

                                                                                                  gTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAg                                   >  2:4890881/1‑140 (MQ=255)

                                                                                                   ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  <  2:2455569/140‑1 (MQ=255)

                                                                                                   ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  <  1:3466802/140‑1 (MQ=255)

                                                                                                   ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  <  1:4214884/140‑1 (MQ=255)

                                                                                                   ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  <  1:4534665/140‑1 (MQ=255)

                                                                                                   ttGGTATCAAAACGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGt                                  <  2:2455728/140‑1 (MQ=255)

                                                                                                            aaaCGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGa                         <  2:1490634/140‑1 (MQ=255)

                                                                                                            aaaCGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGa                         <  2:4729781/140‑1 (MQ=255)

                                                                                                             aaCGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGaa                        <  1:4727001/140‑1 (MQ=255)

                                                                                                              aCGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAag                       <  2:2056627/140‑1 (MQ=255)

                                                                                                              aCGCTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAag                       <  1:1567486/140‑1 (MQ=255)

                                                                                                                 cTACCCACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAgaga                    >  1:4872184/1‑140 (MQ=255)

                                                                                                                    cccACTAATGGAGGGAAACCTATTTTCTTGAAAGTAAAATTCTCGGCGCCGGCATTTTTGCCCCTAAAGTGTTACTCCGGAAGCAGCGAAACGAATCGAACCATTTTCCTCTCGCCAGATGGTTTAGGTTGAAGAGATCg                 <  2:2619291/140‑1 (MQ=255)

                                                                                                                      caatAATGCAGCGAAACCTATTTTCTTGCCAGGAAAAATCTCAGCGACTGCATTTTTGCCCCAAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAAACATTTTCCTCACCCCAGCTAGTATAGGTTGAAGAGATCggg               <  2:1752960/137‑1 (MQ=255)

                                                                                                                      cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  1:856992/140‑1 (MQ=255)

                                                                                                                      cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  1:2267112/140‑1 (MQ=255)

                                                                                                                      cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  1:1086962/140‑1 (MQ=255)

                                                                                                                      cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  2:1312756/140‑1 (MQ=255)

                                                                                                                      cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  2:4396588/140‑1 (MQ=255)

                                                                                                                      cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  1:2042483/140‑1 (MQ=255)

                                                                                                                      cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  1:4046432/140‑1 (MQ=255)

                                                                                                                      cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  1:1880043/140‑1 (MQ=255)

                                                                                                                      cACTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCggg               <  1:3079605/140‑1 (MQ=255)

                                                                                                                       aCTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCgggg              >  2:2957631/1‑140 (MQ=255)

                                                                                                                        cTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGc             <  2:2841284/140‑1 (MQ=255)

                                                                                                                        cTAATGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGc             <  2:1948625/140‑1 (MQ=255)

                                                                                                                          aaTGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGt           <  1:3646692/140‑1 (MQ=255)

                                                                                                                           aTGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGtt          <  1:3344051/140‑1 (MQ=255)

                                                                                                                           aTGGAGGGAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGtt          <  1:4192560/140‑1 (MQ=255)

                                                                                                                           aTGGAGGGAAACCTATTTTCTTCAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAGCCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGtt          <  2:3995529/140‑1 (MQ=255)

                                                                                                                           aTGGAGGGAAACCTATGTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGtt          <  1:1546918/140‑1 (MQ=255)

                                                                                                                                gggAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTaa     <  1:3656112/140‑1 (MQ=255)

                                                                                                                                gggAAACCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTaa     <  2:1543399/140‑1 (MQ=255)

                                                                                                                                   aaaCCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAg  >  2:5073979/1‑140 (MQ=255)

                                                                                                                                   aaaCCTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCGAAAGTGTTACTCCTGAAGCAGCGAAACAGATCGAACCATTTTCCTCTCGGCAGGTAGTTTAGGTTGAAGAGATCGGGGTGTTTGTAagag  >  2:4994870/1‑140 (MQ=255)

AAAAACTGTACAGTTTTGGCTGGGCATAATGATGATTGATTTTACTGCATTGATATAAAAAGAAAAAATGATGCCAAAGCATCATTTTTCCTATCTCAGTTGGTATCAAAACGCTACCCACTAATGGAGGGAAACTTATTTTCTTGAAAGGAAAATTCTCAGCGCCTGCATTTTTGCCCCTAAAGTGTTACTCCTGAAGCAGCGAAACAAATCGAACCATTTTCCTCTCCCCAGATAGTTTAGGTTGAAGAGATCGGGGCGTTTGTAATAG  >  NZ_CP025268/3794613‑3794883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`