BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,943,931	A→G	noncoding (205/1542 nt)	CXP41_RS20515 →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,943,931	0	A	G	98.7%	671.9 / ‑3.2	301	noncoding (205/1542 nt)	CXP41_RS20515	16S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base A (3/1); new base G (146/150); total (150/151)
Fisher's exact test for biased strand distribution p-value = 3.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

TAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTC  >  NZ_CP025268/3943796‑3944067

tAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCtt                                                                                                                                      <  1:3692794/140‑1 (MQ=11)

  aCAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCg                                                                                                                                    >  1:274433/1‑140 (MQ=17)

      gAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGcc                                                                                                                                >  1:2478594/1‑140 (MQ=17)

         aCAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCctct                                                                                                                             >  1:2104809/1‑140 (MQ=18)

          cAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCtt                                                                                                                            >  1:3858146/1‑140 (MQ=18)

          cAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCtt                                                                                                                            <  1:4699386/140‑1 (MQ=18)

              ttGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCa                                                                                                                        >  2:3809730/1‑140 (MQ=17)

               tGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCAt                                                                                                                       <  2:2643793/140‑1 (MQ=17)

                    tttCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGAt                                                                                                                  <  1:4539576/140‑1 (MQ=25)

                       cGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGAtgtg                                                                                                               <  2:1592191/140‑1 (MQ=18)

                         cTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGcc                                                                                                             <  2:549108/140‑1 (MQ=18)

                         cTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGcc                                                                                                             <  1:3214096/140‑1 (MQ=18)

                           gACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGCCCTGTGTCCACCAGATGTGACCa                                                                                                           >  2:479009/1‑140 (MQ=11)

                             cGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGa                                                                                                         <  1:2429700/140‑1 (MQ=18)

                             cGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGa                                                                                                         <  1:723490/140‑1 (MQ=18)

                             cGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGa                                                                                                         <  1:3964420/140‑1 (MQ=17)

                                  ggCGGAAGGGTGAGTAATGTCTGGGAAACCGCCTGATGGAGGGGGATACCTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGa                                                                                                    <  1:4382338/140‑1 (MQ=255)

                                   gCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAt                                                                                                   >  2:2202468/1‑140 (MQ=18)

                                           gTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCtagt                                                                                           >  1:4729967/1‑140 (MQ=18)

                                           gTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTGGCCATCAGATGTGCCTAGATGGGATTAGCtagt                                                                                           >  2:4526040/1‑140 (MQ=12)

                                           gTGAGTAATGTCTGGGAAACTGCCTGAGGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCGTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCtagt                                                                                           >  1:1329868/1‑140 (MQ=255)

                                            tGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCtagta                                                                                          >  1:2670666/1‑140 (MQ=18)

                                              aGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGa                                                                                                    >  2:3226623/1‑128 (MQ=18)

                                              aGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCtagt                                                                                           <  1:828148/137‑1 (MQ=18)

                                              aGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCtagt                                                                                           >  2:828148/1‑137 (MQ=18)

                                              aGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAgg                                                                                        <  2:4646496/140‑1 (MQ=17)

                                              aGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAgg                                                                                        <  2:3829811/140‑1 (MQ=18)

                                               gTAATGCCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAAGACCGCATAACGTAGCAAGACCAAAGAGTGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGt                                                                                       <  2:2537454/140‑1 (MQ=11)

                                                    gTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGt                                                                                  <  1:1012550/140‑1 (MQ=18)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCTTTCGGGCCTCTTGCCATCAGATGTGCACAGATGGGATTAGGCGGAAGGTGGGGtg                                                                                 >  2:428199/1‑139 (MQ=11)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTa                                                                                 >  2:4719985/1‑140 (MQ=17)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCCCTTGCCATCAGATGTGCCCCGACGGGATCGGCTAGCAGGAggggga                                                                                 >  2:2303830/1‑138 (MQ=11)

                                                     tCTGGGAAACTGCCTGATGGAGGGGGATAACTACGGGAAACGGTAGCTAAAACCGCATAACGTCGAAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATAAGCAAGTAGGGGGGGtt                                                                                 >  2:3664309/1‑139 (MQ=11)

                                                        gggAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGtcaag                                                                              >  2:543742/1‑136 (MQ=12)

                                                        gggAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACg                                                                              >  2:2903133/1‑140 (MQ=18)

                                                        gggAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACg                                                                              >  1:4521289/1‑140 (MQ=18)

                                                         ggAACCTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACgg                                                                             <  1:5060252/140‑1 (MQ=16)

                                                         ggAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACgg                                                                             >  2:4834425/1‑140 (MQ=18)

                                                              cTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAc                                                                        >  2:4333076/1‑140 (MQ=18)

                                                              cTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAc                                                                        >  1:289983/1‑140 (MQ=18)

                                                              cTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAc                                                                        <  2:4049735/140‑1 (MQ=18)

                                                               tGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCAcc                                                                       >  1:19377/1‑140 (MQ=18)

                                                                      tGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcga                                                                <  1:3409750/140‑1 (MQ=18)

                                                                      tGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcga                                                                <  1:4781947/140‑1 (MQ=18)

                                                                      tGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAAAGTCGCAAGACCAAAGAGGGGGGCCTTCGGGACTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcga                                                                <  1:3892743/140‑1 (MQ=12)

                                                                       ggAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcgac                                                               <  1:4954570/140‑1 (MQ=18)

                                                                       ggAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcgac                                                               <  2:3959320/140‑1 (MQ=18)

                                                                        gAGGGGGATAACTACTGGAAACGGTAGCTAATAACGCATAACGTCGCAAGACCAAAGAGGGGGATCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcgacg                                                              <  2:1463433/140‑1 (MQ=18)

                                                                         aGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGcgacga                                                             >  1:3502374/1‑140 (MQ=18)

                                                                           ggggATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATc                                                           <  1:2507210/140‑1 (MQ=18)

                                                                           ggggATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATc                                                           >  1:4594665/1‑140 (MQ=18)

                                                                           ggggATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGAGGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATc                                                           >  2:273819/1‑140 (MQ=17)

                                                                              gATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCt                                                        >  1:4171439/1‑140 (MQ=18)

                                                                               aTAACTACTGGAAACGGTAGCGAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGAGGGGATTAGCTAGTAGGTGGGGTAGCGGCTCACCTAGGCGACGATCCCTa                                                       >  2:30123/1‑140 (MQ=255)

                                                                                tAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAg                                                      <  2:713458/140‑1 (MQ=18)

                                                                                tAACTACTGGAAACGGTAGCTAACAACGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCGAGATGGGCTTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAg                                                      <  2:1916175/140‑1 (MQ=11)

                                                                                     actGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGt                                                 >  2:4141150/1‑140 (MQ=17)

                                                                                       tGGAAACGGTAGCTAATACGGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGATCACCTAGGCGACGATCCCTAGCTGGTCt                                               >  2:3574731/1‑140 (MQ=255)

                                                                                         gAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTgagcgga                                             >  2:4475483/1‑134 (MQ=12)

                                                                                           aaCGGTAGCTAATACGGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGCGTAGCTAGGAGGCGGGGTAACAGCTCACCTAGGCGACGAACCCTGGCTGGTAAgaga                                           >  2:5023233/1‑140 (MQ=11)

                                                                                              ggTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGa                                        >  2:3430808/1‑140 (MQ=18)

                                                                                              ggTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGa                                        >  1:976341/1‑140 (MQ=18)

                                                                                              ggTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGa                                        >  1:2079240/1‑140 (MQ=18)

                                                                                              ggTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGAGGGGCCTTCGGGCCTCTTGCCATCAGACGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATGCCTAGCTGGTCTGAGAGGa                                        >  2:1307231/1‑140 (MQ=255)

                                                                                                      ctaacGCATAGCGTAGCACGACCAAAGAGGGGGGCCTTAGGGCCTCTTGCAATCAGATGTCCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCGCTAGCTGGTCTGAGAGGATGACCAGc                                <  1:2800935/136‑1 (MQ=11)

                                                                                                      ctACCGCAGAGCGTAGCAAGGCCAATGTGGGTGGCCTTCGGGCCTCTGGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGCGGGGTAACGGCTCACCTAGGCGAGGATCCCTAGCTGGTCTGAGAGGATGACCAGc                                <  2:4682354/139‑1 (MQ=11)

                                                                                                           gCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCacac                           <  2:2860042/140‑1 (MQ=18)

                                                                                                            cATAACGTCGCAGGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATAAGATGTGACCAGATGGGATTAGCTAGGAGGGGGGGTAACGGCTCACCTAGGCGACGATTCCTAGCTGGTATGAGAGGATGACCAGCCacaca                          >  2:2824671/1‑139 (MQ=11)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGGTGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  2:2467599/1‑140 (MQ=16)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCacacg                          >  1:4504745/1‑139 (MQ=18)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  2:4070198/1‑140 (MQ=18)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  1:3279462/1‑140 (MQ=18)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  1:4023390/1‑140 (MQ=18)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACt                          >  1:5024162/1‑140 (MQ=18)

                                                                                                            cATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGACTCTTGCCATCAGACGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACATAGGCGACGACCCCTAGCTGGTCTGAAAGGATGACCAGCCCCACt                          >  2:4948596/1‑140 (MQ=11)

                                                                                                             aTAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTg                         >  1:666778/1‑140 (MQ=18)

                                                                                                              tcaCGTCTCAAGACCAAAGCGGGGGGCCTTCCGTACTCTTGCCATCAGATGTGCCCAGATGGGATTCGCTAGTAGGTGGGGTAAAGACTCCCCTGGGCGACCATCCCTAGCTGGCCTGAGAGGATGACCAGCCCCACTgg                        <  2:2181213/138‑1 (MQ=11)

                                                                                                              tAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTgg                        <  2:3539266/140‑1 (MQ=18)

                                                                                                              tAACGTCGCAAGACCAAAGAGGGGGGACTTCGGGCCTCTTGCCATCAGATGTGCCCAGGGGGGGGTAGCTAGTAGGTGGGGGCACGGCTCACCTAGGCCACGATCCTTAGCTGGTCTGGGGGGAGGACCAGCCACACTgg                        >  2:1124468/1‑140 (MQ=11)

                                                                                                                    cGCAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCGATCAGATGTGCCCAGATGGGAGTAGCTAGTAGGTGGGGTCACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGCGAGGATGACCCGCCACACTGGAACTga                  >  2:2774716/1‑140 (MQ=11)

                                                                                                                      cAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTgaga                >  1:226925/1‑140 (MQ=18)

                                                                                                                      cAAGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTgaga                >  1:3546894/1‑140 (MQ=18)

                                                                                                                       aaGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGac               <  1:2386690/140‑1 (MQ=18)

                                                                                                                        aGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGaca              <  2:48985/140‑1 (MQ=18)

                                                                                                                        aGACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGaca              <  1:1826906/140‑1 (MQ=18)

                                                                                                                          aCCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACg            <  2:1853435/140‑1 (MQ=18)

                                                                                                                            cAAAGAGGGGGGCCCTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACCGGAACGGAGAGACggg          >  2:5055220/1‑139 (MQ=11)

                                                                                                                             aaaGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTc         <  1:3780119/140‑1 (MQ=18)

                                                                                                                              aaGAGGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTcc        >  1:1421425/1‑140 (MQ=18)

                                                                                                                               agagGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTGGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCa       <  1:1615462/140‑1 (MQ=16)

                                                                                                                               agagGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCa       <  1:3057846/140‑1 (MQ=18)

                                                                                                                               agagGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCa       <  1:2603904/140‑1 (MQ=18)

                                                                                                                               agagGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCa       >  1:584643/1‑140 (MQ=18)

                                                                                                                                 agGGGGGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGa     <  1:1176697/140‑1 (MQ=18)

                                                                                                                                  ggggggCCTTCGTGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGAc    <  2:2502764/140‑1 (MQ=18)

                                                                                                                                   ggggGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGTGAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACt   >  2:3066539/1‑140 (MQ=12)

                                                                                                                                   ggggGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACt   >  1:4199996/1‑140 (MQ=18)

                                                                                                                                   ggggGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACt   >  1:4837568/1‑140 (MQ=18)

                                                                                                                                   ggggGCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGGGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACt   >  2:2053315/1‑140 (MQ=12)

                                                                                                                                    ggggCCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTTGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTc  >  1:2693100/1‑140 (MQ=18)

TAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGATTAGCTAGTAGGTGGGGTAACGGCTCACCTAGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACTGAGACACGGTCCAGACTC  >  NZ_CP025268/3943796‑3944067

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`