BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,963,836	C→G	A414G (GCC→GGC)	rep →	DNA helicase Rep

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,963,836	0	C	G	99.7%	999.3 / NA	304	A414G (GCC→GGC)	rep	DNA helicase Rep
Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (131/172); minor base A (0/1); total (131/173)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 309 total aligned reads displayed.

TACAAAATATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGCCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGCTTCACTCACTG  >  NZ_CP025268/3963700‑3963971

tACAAAATATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGgccc                                                                                                                                      >  1:2866763/1‑140 (MQ=255)

tACAAAATATCTGGTGGTACGTCG‑TTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCg                                                                                                                                     >  2:3543127/1‑140 (MQ=255)

     aaTATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTa                                                                                                                                 <  1:1953648/140‑1 (MQ=255)

      atatCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTAc                                                                                                                                <  1:399449/140‑1 (MQ=255)

      atatCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTAc                                                                                                                                <  1:4273043/140‑1 (MQ=255)

      atatCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTAc                                                                                                                                <  1:5020192/140‑1 (MQ=255)

      atatCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTAc                                                                                                                                <  1:545622/140‑1 (MQ=255)

       tatCTGGTGGTACGCCGTTTTTCTCTCGTCCTGGAAGCGAGGACTTGCGGGCTTATCTTCGCGTGCTG‑ACTGCCCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGGAGCGAGAGATTGGCCGGGCTAcc                                                                                                                               <  2:2310519/140‑2 (MQ=255)

        atCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGc                                                                                                                              >  2:848655/1‑140 (MQ=255)

        atCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGc                                                                                                                              >  1:2767163/1‑140 (MQ=255)

         tCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCt                                                                                                                             >  1:4014393/1‑140 (MQ=255)

          cTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTg                                                                                                                            >  1:2143194/1‑140 (MQ=255)

          cTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTg                                                                                                                            >  2:4135542/1‑140 (MQ=255)

          cTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTg                                                                                                                            >  2:1140392/1‑140 (MQ=255)

          cTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGCCTTATCTGCGCGTGCTG‑ACTAACCCGGGCGATGACAGCGCATTTCTGGGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTg                                                                                                                            >  2:4006644/1‑140 (MQ=255)

            ggtggtACGTCGTTTTTCTCTCGTCCTGCAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTACCCCGGACGGTGACAGCGCATTTCTGCGTATCGTGAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGaa                                                                                                                          <  2:2037991/140‑1 (MQ=255)

               ggtACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGaaaaa                                                                                                                       >  2:129504/1‑140 (MQ=255)

                gtACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTCCGCTGAAAAAg                                                                                                                      <  1:752046/140‑1 (MQ=255)

                 tACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGc                                                                                                                     >  2:4711404/1‑140 (MQ=255)

                     tcgtTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTggg                                                                                                                 <  2:2474154/140‑1 (MQ=255)

                      cgtTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGt                                                                                                                <  1:89374/140‑1 (MQ=255)

                      agtatttCTCTCATCCTGAAATCAAGGCCTTGCTGGCTTATCTCCGCGTTCTG‑ACTAACCCGGACCATGACAGCGCATCTCTGCGTCCCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAACCTCGGt                                                                                                                <  2:4052368/136‑1 (MQ=255)

                        tctttCTCTCGTCCTGCAATCAATGACTTGCTCGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGCATCGTTACCACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGCAAAAGCTGGGTGa                                                                                                              <  2:3346610/138‑1 (MQ=255)

                         ttttCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAg                                                                                                             <  1:2711606/140‑1 (MQ=255)

                          tttCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGt                                                                                                            >  2:1813283/1‑140 (MQ=255)

                                  gTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATg                                                                                                    >  1:1967356/1‑140 (MQ=255)

                                  gTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGGGAGTGGGCGATg                                                                                                    >  1:1331720/1‑140 (MQ=255)

                                     cTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTACCCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGAcg                                                                                                 <  1:3901452/140‑1 (MQ=255)

                                     cTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGAcg                                                                                                 <  2:3720432/140‑1 (MQ=255)

                                            cAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATa                                                                                          <  1:3530482/140‑1 (MQ=255)

                                             aaGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATaa                                                                                         >  2:3252198/1‑140 (MQ=255)

                                              aGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTGAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATaaa                                                                                        >  2:4540433/1‑140 (MQ=255)

                                               ggACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAACGCTGGGTGAGTGGGCGATGACGCGCAATaaaa                                                                                       >  2:3183310/1‑140 (MQ=255)

                                                  cttgcttgCCTATCGCCGCG‑GCTGTACTAACCCGGACGATGCCGGCGCATTGGTGCGTATCGCTACCATGCCCAGGCGAGAGATTGGCCCGGCTGCGCTGAAAAAGCTGGGTGAGTGCGCGATGATGGGTAATAAATGCa                                                                                    <  2:4867861/140‑1 (MQ=255)

                                                  cTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCa                                                                                    <  2:3593770/140‑1 (MQ=255)

                                                    tGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATg                                                                                  >  2:2684869/1‑140 (MQ=255)

                                                     gCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATATAAGCATGt                                                                                 >  1:514467/1‑140 (MQ=255)

                                                     gCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGt                                                                                 >  2:4917832/1‑140 (MQ=255)

                                                     gCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGt                                                                                 >  1:2539837/1‑140 (MQ=255)

                                                             gtCTGCGCGTGCTG‑ACTAACCTGGACGATGACAGCGCATTTCTGCGTATCGTTACCACGCTGAAGCGAGAGATTGGGCCGGCTACGCTGAAAAAGCTGGCGGAGTGGGCGACGACGCGCAATAGAGGCATGTATACCGcc                                                                         >  2:4226894/2‑140 (MQ=255)

                                                                tGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGc                                                                      >  1:4768312/1‑140 (MQ=255)

                                                                  cgcgTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCtt                                                                    <  2:1939770/140‑1 (MQ=255)

                                                                   gcgTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCttt                                                                   >  2:3463500/1‑140 (MQ=255)

                                                                    cgTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTg                                                                  >  2:687029/1‑140 (MQ=255)

                                                                    cgTGCTG‑ACTAACCCGGACGATGAAAGCGGATTTCTGCGTATCGTTAACAGGCCGAAGCGAGGGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCCATAAAAGCATGTTTACCGTCTGCTTTg                                                                  >  2:2370371/1‑140 (MQ=255)

                                                                      tGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGat                                                                <  1:1431347/140‑1 (MQ=255)

                                                                       gCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGATATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                               <  2:3382151/140‑1 (MQ=255)

                                                                       gCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                               <  2:301915/140‑1 (MQ=255)

                                                                       gCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                               <  1:3099304/140‑1 (MQ=255)

                                                                       gCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                               <  1:181762/140‑1 (MQ=255)

                                                                       gCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                               <  1:4196392/140‑1 (MQ=255)

                                                                       gCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                               <  1:111483/140‑1 (MQ=255)

                                                                       gCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                               <  2:3305775/140‑1 (MQ=255)

                                                                       gCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGata                                                               <  2:1692511/140‑1 (MQ=255)

                                                                        cTG‑CCTAACACGGCCGATGGGAGCGCCTCTCTGCGTATCGTTCGCACACCGAAGCGAGAGCCTGGCCCGGGTCCGCTGACAACGCGGGGTGAGTGGGCGATGACGCGAAATAAAAGCATGTTTACCGCCAGCTTTGatat                                                              <  2:1619177/140‑1 (MQ=255)

                                                                        cTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGatat                                                              >  2:2577275/1‑140 (MQ=255)

                                                                        cTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGatat                                                              >  1:4124990/1‑140 (MQ=255)

                                                                        cTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGatat                                                              <  2:1477497/140‑1 (MQ=255)

                                                                        cTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGatat                                                              <  2:2918637/140‑1 (MQ=255)

                                                                             cTAACCCGGACGAGGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGCCGCTGACGGGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGc                                                          <  1:2249876/140‑1 (MQ=255)

                                                                               aaCCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCt                                                        <  1:676482/140‑1 (MQ=255)

                                                                                 cccGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGa                                                      >  1:1843833/1‑140 (MQ=255)

                                                                                 cccGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGGGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGa                                                      >  2:605080/1‑140 (MQ=255)

                                                                                      aCGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAg                                                 >  2:2531083/1‑140 (MQ=255)

                                                                                      aCGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAg                                                 >  1:4828074/1‑140 (MQ=255)

                                                                                               gcgcATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGc                                        >  1:322563/1‑140 (MQ=255)

                                                                                                cgcATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCg                                       >  1:1570028/1‑140 (MQ=255)

                                                                                                cgcATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCGTAGCg                                       >  1:2440756/1‑140 (MQ=255)

                                                                                                 gcATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCgg                                      <  1:1731622/140‑1 (MQ=255)

                                                                                                  cATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGa                                     <  1:1289090/140‑1 (MQ=255)

                                                                                                  cATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGa                                     <  1:3699577/140‑1 (MQ=255)

                                                                                                    tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                   <  2:3800188/140‑1 (MQ=255)

                                                                                                    tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                   <  2:4829519/140‑1 (MQ=255)

                                                                                                    tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                   <  2:934553/140‑1 (MQ=255)

                                                                                                    tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                   <  1:1245840/140‑1 (MQ=255)

                                                                                                    tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                   <  1:3398615/140‑1 (MQ=255)

                                                                                                    tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                   <  1:4721319/140‑1 (MQ=255)

                                                                                                    tttCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACg                                   <  1:3496174/140‑1 (MQ=255)

                                                                                                     ttCTGCGTATCGTGAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGGGGGCGATGACGCGCAATAAAAGCCTGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGt                                  <  2:427282/140‑1 (MQ=255)

                                                                                                       cTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTgg                                <  2:532383/140‑1 (MQ=255)

                                                                                                       cTGCGTATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTgg                                <  1:31276/140‑1 (MQ=255)

                                                                                                            tATCGTTAACACGCCGAAGCGAGGGATTGGCCCGGCTACGCGGAACAAGCTGCGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCCGCCTCGATATGGGCCCGAGCCAGACGCTTAGCGGACTTGGtactg                           >  2:3855097/1‑136 (MQ=255)

                                                                                                            tATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATg                           <  1:1015418/140‑1 (MQ=255)

                                                                                                            tATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATg                           <  1:2891065/140‑1 (MQ=255)

                                                                                                            tATCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATg                           <  1:3830165/140‑1 (MQ=255)

                                                                                                             aTCGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATgt                          >  1:4504693/1‑139 (MQ=255)

                                                                                                               cGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAg                        >  1:95689/1‑140 (MQ=255)

                                                                                                               cGTTAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAg                        >  1:1490524/1‑140 (MQ=255)

                                                                                                                 ttAACACGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCa                      <  1:4609123/140‑1 (MQ=255)

                                                                                                                      acGCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGAc                 <  2:1066507/140‑1 (MQ=255)

                                                                                                                        gCCGAAGCGAGGGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTGACCGCCGGCTTTGATATGGGCCTGAGTCAGAGGCTTAGCGGGTGGTGTTATGAAGCATTGAccc               >  2:1374508/1‑140 (MQ=255)

                                                                                                                        gCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGAccc               >  2:591523/1‑140 (MQ=255)

                                                                                                                        gCCGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGAccc               <  1:1548868/140‑1 (MQ=255)

                                                                                                                         ccGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCg              >  1:1645182/1‑140 (MQ=255)

                                                                                                                         ccGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCg              >  2:2878515/1‑140 (MQ=255)

                                                                                                                          cGAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGc             >  2:1511461/1‑140 (MQ=255)

                                                                                                                           gAAGCGAGAGATTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGCt            <  1:3444801/140‑1 (MQ=255)

                                                                                                                                  gagaTTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGTGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGGATTGACCCGCTtcactca     <  2:1389274/140‑1 (MQ=255)

                                                                                                                                  gagaTTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGCTtcactca     <  1:826026/140‑1 (MQ=255)

                                                                                                                                  gagaTTGGCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGCTtcactca     >  2:875610/1‑140 (MQ=255)

TACAAAATATCTGGTGGTACGTCGTTTTTCTCTCGTCCTGAAATCAAGGACTTGCTGGCTTATCTGCGCGTGCTG‑ACTAACCCGGACGATGACAGCGCATTTCTGCGTATCGTTAACACGCCGAAGCGAGAGATTGCCCCGGCTACGCTGAAAAAGCTGGGTGAGTGGGCGATGACGCGCAATAAAAGCATGTTTACCGCCAGCTTTGATATGGGCCTGAGTCAGACGCTTAGCGGACGTGGTTATGAAGCATTGACCCGCTTCACTCACTG  >  NZ_CP025268/3963700‑3963971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`