BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,978,525	C→T	L110F (CTT→TTT)	wzxE →	lipid III flippase WzxE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,978,525	0	C	T	98.6%	1235.3 / NA	352	L110F (CTT→TTT)	wzxE	lipid III flippase WzxE
Reads supporting (aligned to +/- strand): ref base C (0/1); major base T (189/156); minor base G (3/2); total (192/160)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 354 total aligned reads displayed.

AATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCG  >  NZ_CP025268/3978388‑3978660

 aTACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACGTCATCAGCGATGGTACTTGGTTTCTCGACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTg                                                                                                                                      <  1:4767738/140‑1 (MQ=255)

  tACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTgg                                                                                                                                     >  1:3444852/1‑140 (MQ=255)

  tACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTgg                                                                                                                                     >  1:3770932/1‑140 (MQ=255)

  tACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTgg                                                                                                                                     >  1:1373443/1‑140 (MQ=255)

  tACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTgg                                                                                                                                     >  2:2332766/1‑140 (MQ=255)

    cGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTa                                                                                                                                   >  2:4150325/1‑140 (MQ=255)

     gTTGCCCCGTCCCATGACACTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCCGCGCTGGTCCTTGGTTTCTCTACGCTGATGGAGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTaa                                                                                                                                  <  2:651951/140‑1 (MQ=255)

       tGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGTGATTGTTTGGTAATa                                                                                                                                >  2:1468784/1‑140 (MQ=255)

       tGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATa                                                                                                                                <  2:4206285/140‑1 (MQ=255)

       tGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATa                                                                                                                                <  1:1662146/140‑1 (MQ=255)

       tGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATa                                                                                                                                >  2:2522716/1‑140 (MQ=255)

        gCCCCGTAATATGATAATTCGCAACAGCTGCGCCGCGTGGCTGGGACTTTATCATCGATGGTACTTGGTTTCTGTACGATGATGGCGCTGTTTTTTGTGCTGGCAGCTGCGCCATTCATCCAGGGATTGTTTGGTACTat                                                                                                                               >  2:3014561/1‑139 (MQ=255)

          ccAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCg                                                                                                                             <  2:3213035/140‑1 (MQ=255)

           cAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGa                                                                                                                            <  2:4585849/140‑1 (MQ=255)

           cAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGa                                                                                                                            <  1:4884164/140‑1 (MQ=255)

            aGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGAc                                                                                                                           <  2:4267767/140‑1 (MQ=255)

              tACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTa                                                                                                                         >  1:4999240/1‑140 (MQ=255)

              tACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATAAGCGATGGTACGTGGTTTCTCTACGCTGATGGCGCGGGGTGTTGTGCTGGCAGCTGCGCCAATCAGGCAGGGATTGTGTGGTAATACCGACTa                                                                                                                         >  2:1867478/1‑140 (MQ=255)

                ccATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATc                                                                                                                       <  1:4275661/140‑1 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTCCTTGGTTTCTCTACGCTGATGGCGCTGGTGATTGTGCTGGCAGCTGCGCAAATCAGACAGGGATTGTTGGGTAATAACGACTATCAgg                                                                                                                    >  2:1960847/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCTACTCTCAgg                                                                                                                    >  2:592567/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                    >  2:4112669/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                    >  2:2789273/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                    >  1:3742827/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                    >  1:593155/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                    >  1:2991937/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                    >  2:747666/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                    >  1:1870743/1‑140 (MQ=255)

                   tGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                    >  1:2103563/1‑140 (MQ=255)

                   tGATAATACGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTAGTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGGGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAgg                                                                                                                    <  2:474150/140‑1 (MQ=255)

                   tGATAAGCCGCAACAGCTGCGCCGCGTGGTCCGCACTTCATACGCGATGGTACTTGGGCTCTCGACGCTGATGGCGCTGGTGTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTAGTCATACCGACTATAAgg                                                                                                                    >  2:3647130/1‑140 (MQ=255)

                        aTCCGCAACAGCTGCGCCGCGTGGTCGGCGCTTCATAAGCGATGGTACTTGGTTTCTATACCATGATGGCGCGGGGTGTTGTGCTGGCAGCTGCGCCACTCAGCCAGGGATTGTTTGTTATTACGGACGATCAGGTGCTg                                                                                                               >  2:480698/1‑140 (MQ=255)

                        aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTg                                                                                                               <  2:216865/140‑1 (MQ=255)

                        aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTg                                                                                                               >  2:1301077/1‑140 (MQ=255)

                        aTCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGCTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTg                                                                                                               >  1:1555878/1‑140 (MQ=255)

                          ccGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGt                                                                                                             >  2:3469600/1‑140 (MQ=255)

                          ccGCAACAGCTGCCCCCCGTGGCCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGt                                                                                                             <  2:4020585/140‑1 (MQ=255)

                           cGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTg                                                                                                            >  1:4058882/1‑140 (MQ=255)

                                   cTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGt                                                                                                    <  1:3242464/140‑1 (MQ=255)

                                       gccgcGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGgcg                                                                                                <  1:5059087/140‑1 (MQ=255)

                                       gccgcGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGgcg                                                                                                <  2:2499613/140‑1 (MQ=255)

                                        ccgcGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGgcgc                                                                                               <  2:2720309/140‑1 (MQ=255)

                                        ccgcGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGgcgc                                                                                               <  1:3593596/140‑1 (MQ=255)

                                           cgTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTgg                                                                                            <  1:2154376/140‑1 (MQ=255)

                                           cgTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTgg                                                                                            <  1:28722/140‑1 (MQ=255)

                                             tGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                                          <  2:4999240/140‑1 (MQ=255)

                                             tGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                                          <  1:2552179/140‑1 (MQ=255)

                                             tGGTCGGCACTTCATCAGCGATGGTACTTGGTCTCTCTACGCTGATGGCGCTGGTTGTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTg                                                                                          <  1:3633330/140‑1 (MQ=255)

                                                      cTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATgggg                                                                                 <  2:2834586/140‑1 (MQ=255)

                                                      cTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATgggg                                                                                 <  2:1122287/140‑1 (MQ=255)

                                                       ttCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGTGCCAATCAGCCAGGGATTGTTTGGTAATACAGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCGAATGGGGa                                                                                <  2:4488567/140‑1 (MQ=255)

                                                       ttCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGa                                                                                <  1:4501424/140‑1 (MQ=255)

                                                       ttCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGa                                                                                <  1:1009/140‑1 (MQ=255)

                                                       ttCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGa                                                                                <  1:2353485/140‑1 (MQ=255)

                                                       ttCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGa                                                                                <  1:79262/140‑1 (MQ=255)

                                                         catcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATc                                                                              >  1:903310/1‑140 (MQ=255)

                                                          ctgaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTGTAGTGGCGCTGGTGCACATGGGGATCg                                                                             >  2:1207511/4‑140 (MQ=255)

                                                          atcaGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCg                                                                             >  2:2429496/1‑140 (MQ=255)

                                                                 ctGGTACTTGGTTTCTCTACGCTGATGGCGCGGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGTATTGTTTGGTAATACCGACTATCAGGGGCTGCTGCGTTTAGTGGCGCTGGTGCAAATGGGGAGCGCCTgggg                                                                      <  2:312668/139‑1 (MQ=255)

                                                                 aTGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATGCCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCACATGGGGATCGCCTgggg                                                                      >  2:3373871/1‑140 (MQ=255)

                                                                 aTGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTgggg                                                                      >  2:1531225/1‑140 (MQ=255)

                                                                     tACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAAc                                                                  <  1:1312363/140‑1 (MQ=255)

                                                                          ggTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGtt                                                             >  1:3624971/1‑140 (MQ=255)

                                                                            tttCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTAc                                                           >  2:739572/1‑140 (MQ=255)

                                                                              tctcTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGAGTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTg                                                         >  1:3941095/1‑140 (MQ=255)

                                                                               ctctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTgg                                                        >  1:1451024/1‑140 (MQ=255)

                                                                               ctctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTgg                                                        >  1:4647493/1‑140 (MQ=255)

                                                                                 ctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCCGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGgcg                                                      <  1:3071998/140‑1 (MQ=255)

                                                                                 ctACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGgcg                                                      <  2:883739/140‑1 (MQ=255)

                                                                                  tACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGgcgc                                                     <  1:4924372/140‑1 (MQ=255)

                                                                                  aacacTGATGTTGCTGATTTTTGTGCTGGAAGCTTCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTGTCAGGGGTTGGTGCGTTGAGTGGCGCTGGTGCAAATGGGTATCGCCTGTGGCAACCTGTTACTGgcgc                                                     <  2:1752172/136‑1 (MQ=255)

                                                                                   ccGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCt                                                    <  2:2678012/139‑1 (MQ=255)

                                                                                    cGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCtg                                                   >  2:4649799/1‑140 (MQ=255)

                                                                                      cTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGTATCGTTTGGTAATACCGACTATCAGAGGCTGGTGCGGTGAGTTGCGCTGGTGCACATGGGGCTCCCCGGGGGCAAGCTGTTACTGGCGCtgat                                                 >  2:36755/1‑140 (MQ=255)

                                                                                         aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                              >  1:219191/1‑140 (MQ=255)

                                                                                         aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                              >  2:4314624/1‑140 (MQ=255)

                                                                                         aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                              >  1:4021047/1‑140 (MQ=255)

                                                                                         aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                              >  1:4227678/1‑140 (MQ=255)

                                                                                         aTGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGaa                                              >  2:3866517/1‑140 (MQ=255)

                                                                                          tGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGGTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGgaa                                             >  2:1050793/1‑140 (MQ=255)

                                                                                           ggCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAg                                            <  2:3589156/140‑1 (MQ=255)

                                                                                              gcTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCt                                         >  1:1050442/1‑140 (MQ=255)

                                                                                                tGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCttt                                       <  1:1365142/140‑1 (MQ=255)

                                                                                                    ttttGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcgcg                                   <  1:2938440/140‑1 (MQ=255)

                                                                                                      ttGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTcgcgct                                 >  2:2977783/1‑138 (MQ=255)

                                                                                                          gCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCg                             <  2:3783463/140‑1 (MQ=255)

                                                                                                          gCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCg                             <  1:919765/140‑1 (MQ=255)

                                                                                                          gCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCg                             <  1:2789273/140‑1 (MQ=255)

                                                                                                          gCTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGGAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCg                             <  1:5031830/140‑1 (MQ=255)

                                                                                                           cTGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGc                            <  1:4696055/140‑1 (MQ=255)

                                                                                                            tGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCa                           >  1:2725622/1‑140 (MQ=255)

                                                                                                            tGGCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGGTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCa                           >  1:4147143/1‑140 (MQ=255)

                                                                                                             ggCAGCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTCTCAGGGGCTGGTGCGTTTAGTGGCGCTGCGGCAAATGGGGCTCCCCTGGGGCAACCTGCTCCTGGCGCCGACGACACGCCTTCGCGACGCCCCac                          >  2:5066207/1‑139 (MQ=255)

                                                                                                                 gCTGCGCCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTaa                      <  1:2465301/140‑1 (MQ=255)

                                                                                                                     cgcCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCg                  <  1:262627/140‑1 (MQ=255)

                                                                                                                     cgcCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCg                  >  1:2053459/1‑140 (MQ=255)

                                                                                                                     cgcCAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGATGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCg                  <  1:12181/140‑1 (MQ=255)

                                                                                                                       ccAATCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGtt                <  1:1770975/140‑1 (MQ=255)

                                                                                                                         aaTCAGCCAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTAt              <  2:205323/140‑1 (MQ=255)

                                                                                                                               ccAGGGATTGTTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGa        >  1:67130/1‑140 (MQ=255)

AATACGTTGCCCAGTACCATGATAATCCGCAACAGCTGCGCCGCGTGGTCGGCACTTCATCAGCGATGGTACTTGGTTTCTCTACGCTGATGGCGCTGGTTTTTGTGCTGGCAGCTGCGCCAATCAGCCAGGGATTGCTTGGTAATACCGACTATCAGGGGCTGGTGCGTTTAGTGGCGCTGGTGCAAATGGGGATCGCCTGGGGCAACCTGTTACTGGCGCTGATGAAAGGCTTTCGCGATGCCGCAGGTAATGCGTTATCGCTGATTGTCG  >  NZ_CP025268/3978388‑3978660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`