Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 4,206,567 A→G *442* (TAA→TAG) 
N429N (AAT→AAC) 		 zraR →
purD ←		 sigma‑54‑dependent response regulator transcription factor ZraR
phosphoribosylamine‑‑glycine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252684,206,5670AG99.7% 1042.0 / NA 315*442* (TAA→TAG) 
N429N (AAT→AAC) 		zraR
purD		sigma‑54‑dependent response regulator transcription factor ZraR
phosphoribosylamine‑‑glycine ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (95/219);  minor base C (0/1);  total (95/220)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 315 total aligned reads displayed.

AGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCACAGTA  >  NZ_CP025268/4206431‑4206703
                                                                                                                                        |                                                                                                                                        
 ggATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCt                                                                                                                                      >  1:2330328/1‑140 (MQ=255)
   atatTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGc                                                                                                                                    <  1:1494656/140‑1 (MQ=255)
   atatTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCCGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGAAAACCTGTCGCGTTAGTTCTGc                                                                                                                                    <  2:4302222/140‑1 (MQ=255)
     atTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTc                                                                                                                                  <  2:3949062/140‑1 (MQ=255)
     atTCAGCCGTAGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACTGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGTGATCACGCGCAAAACGCTATGGGCAAAACTGTCGCGTTAGTTCTGCTc                                                                                                                                  <  2:3736542/140‑1 (MQ=255)
        cAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTcgcg                                                                                                                               <  1:4233764/140‑1 (MQ=255)
              ttGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGAt                                                                                                                         >  2:522312/1‑140 (MQ=255)
              ttGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGAt                                                                                                                         >  2:2076468/1‑140 (MQ=255)
                ggtggAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAg                                                                                                                       >  1:3675266/1‑140 (MQ=255)
                     aagtggaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAgcgcgc                                                                                                                  <  2:3220600/140‑1 (MQ=255)
                     aagtggaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAgcgcgc                                                                                                                  >  2:4838216/1‑140 (MQ=255)
                     aagtggaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAgcgcgc                                                                                                                  <  1:69683/140‑1 (MQ=255)
                     aagtggaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAgcgcgc                                                                                                                  >  1:452445/1‑140 (MQ=255)
                     aagtggaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAgcgcgc                                                                                                                  >  1:4056729/1‑140 (MQ=255)
                     aagtggaaAAAGAGGCGCTTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAACCCGAAGCCCCCCGTCAGTTAGGGATCACGCGAAAAACCCTATTGGCAAAACTGTCGCGTTAGTCCTGCTCGCGTTCGATAgcgcgc                                                                                                                  <  2:1693085/140‑1 (MQ=255)
                          gaaAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGc                                                                                                                                    >  1:549628/1‑117 (MQ=255)
                           aaaaaGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCg                                                                                                            >  1:3809272/1‑140 (MQ=255)
                           aaaaaGAGGTGATTCTGGCGGCGATGGAGAAAACGGGCGGCAACAAAACCGTAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCg                                                                                                            >  1:3056144/1‑140 (MQ=255)
                            aaaaGAGGGGAGTCTGGCGGCGCTGGAGAGAGCTGTCGGCAAGAAAACCGAAGCGGCCCGTCGGTTAGGGATCACGCGCAAAACGCTGTTGGCAAGGCGGTCGCGGTAGTTCTGATAGCAGTCGGGAGCGCGCCatacga                                                                                                           >  2:653957/1‑135 (MQ=255)
                                  ggTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCtt                                                                                                     >  1:2567177/1‑140 (MQ=255)
                                    tGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTc                                                                                                   <  2:1233499/140‑1 (MQ=255)
                                               gcgcTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCGGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCa                                                                                        >  2:4667520/1‑140 (MQ=255)
                                                cgcTGGAGAAAACGGGCGGCAACAAAACCGCAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAg                                                                                       <  1:2159325/140‑1 (MQ=255)
                                                cgcTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAg                                                                                       <  2:817480/140‑1 (MQ=255)
                                                 gcTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt                                                                                      <  1:918814/140‑1 (MQ=255)
                                                 gcTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt                                                                                      <  1:1968619/140‑1 (MQ=255)
                                                 gcTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgg                                                                                      >  2:1928386/1‑139 (MQ=255)
                                                 gcTGGAGAAAACGGGCCGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGCTCCCGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgt                                                                                      <  2:1417645/140‑1 (MQ=255)
                                                     gagaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAACCGATATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                                  <  2:2422237/140‑1 (MQ=255)
                                                     gagaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAgtcgtc                                                                                  <  1:4755568/140‑1 (MQ=255)
                                                      agaAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTcc                                                                                 <  1:5050733/140‑1 (MQ=255)
                                                        aaaaCGGGGCGCAAGAAACCCGAAGCCGCCCGTCCGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGCTAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCa                                                                               <  2:1947370/140‑1 (MQ=255)
                                                        aaaaCGGGCGGCAACAAACCCGAATCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCTTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCa                                                                               <  2:3524913/140‑1 (MQ=255)
                                                           aCGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCCCGCGCACAACGCTCTTGGCAAAACTGTCGCGTTAGTTCTGCTCGAGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGACGCAGTCGTCCCAGTg                                                                            >  2:2137386/1‑140 (MQ=255)
                                                           aCGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTg                                                                            >  1:1539022/1‑140 (MQ=255)
                                                             gggCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGa                                                                          <  1:814095/140‑1 (MQ=255)
                                                               gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGGAAAACGCTATTGGCAAAACTGGCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTGCCGGCAGAAGCAGTCGTCCCAGTGGata                                                                        >  1:511923/1‑140 (MQ=255)
                                                               gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGata                                                                        >  1:4437386/1‑140 (MQ=255)
                                                               gcggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGata                                                                        >  1:2627897/1‑140 (MQ=255)
                                                                cggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGatat                                                                       >  2:4468403/1‑140 (MQ=255)
                                                                 ggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATc                                                                      <  1:332626/140‑1 (MQ=255)
                                                                 ggcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATc                                                                      <  2:4327187/140‑1 (MQ=255)
                                                                  gcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                                     <  2:750051/140‑1 (MQ=255)
                                                                  gcAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCg                                                                     <  1:2956814/140‑1 (MQ=255)
                                                                    aacaaAACCGAAGCCGCGCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGGGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGt                                                                   <  2:4025691/140‑1 (MQ=255)
                                                                         acccGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTa                                                              <  2:2005505/138‑1 (MQ=255)
                                                                           ccGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAg                                                            <  2:2703063/140‑1 (MQ=255)
                                                                               aGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCAt                                                        <  2:266024/140‑1 (MQ=255)
                                                                                 ccgccCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAg                                                      <  2:3098621/140‑1 (MQ=255)
                                                                                  cgccCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAgg                                                     <  1:281200/140‑1 (MQ=255)
                                                                                   gccCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGgc                                                    >  1:4604020/1‑140 (MQ=255)
                                                                                    cccGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGgcg                                                   <  2:2219874/140‑1 (MQ=255)
                                                                                             tAGGGCTCCCGCGCAAAACGCTATTGGCAAAGCTGTCTCGTTAGGTCTGCTCGCGGTCGAGCGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGtactgtg                                          <  2:579762/140‑7 (MQ=255)
                                                                                                    cACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTg                                   >  1:1712897/1‑140 (MQ=255)
                                                                                                     ccgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                                  <  2:4863497/139‑1 (MQ=255)
                                                                                                     ccgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                                  <  1:841485/139‑1 (MQ=255)
                                                                                                     aCGCGCAAACCGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTACGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                                  <  1:1357534/140‑1 (MQ=255)
                                                                                                     aCGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                                  <  1:3491093/140‑1 (MQ=255)
                                                                                                     aCGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                                  <  1:1163792/140‑1 (MQ=255)
                                                                                                     aCGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                                  <  2:5014312/140‑1 (MQ=255)
                                                                                                     aCGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                                  <  1:4903873/140‑1 (MQ=255)
                                                                                                     aCGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                                  <  1:4673503/140‑1 (MQ=255)
                                                                                                     aCGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAAAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGc                                  >  1:4064207/1‑140 (MQ=255)
                                                                                                      cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                                 >  2:3773573/1‑140 (MQ=255)
                                                                                                      cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                                 >  2:116305/1‑140 (MQ=255)
                                                                                                      cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                                 >  2:115793/1‑140 (MQ=255)
                                                                                                      cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                                 <  1:1308464/140‑1 (MQ=255)
                                                                                                      cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                                 >  1:2698080/1‑140 (MQ=255)
                                                                                                      cgcgCAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                                 >  1:3141153/1‑140 (MQ=255)
                                                                                                      cgcgCAAAACGCTATTGGCAAAACTGTCGCGGTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGcc                                 >  2:280147/1‑140 (MQ=255)
                                                                                                       gcgcAAATCGCTATTGACAAAACTGTGGTGTTGGTTCTGCACGCGTTCGATGGCGCGCCAGCCGTTATCTTTCCGGCAGGAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGTCGCGTTTCTGCGCTTCTGccc                                <  2:4224677/140‑2 (MQ=255)
                                                                                                       gcgcAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  2:1791449/140‑1 (MQ=255)
                                                                                                       gcgcAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  1:996319/140‑1 (MQ=255)
                                                                                                       gcgcAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  1:3263574/140‑1 (MQ=255)
                                                                                                       gcgcAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  1:2633429/140‑1 (MQ=255)
                                                                                                       gcgcAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  1:4167592/140‑1 (MQ=255)
                                                                                                       gcgcAAAACGCTATTGGCAAAACTGTCGCATTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCa                                <  2:3576063/140‑1 (MQ=255)
                                                                                                          cAAAACGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACgg                             >  1:1082615/1‑140 (MQ=255)
                                                                                                            cgaCGCTATTGTCAAAACTACAGCGTTTGATCTACTGGCGATCGACAGCTCGCCCGCCGATATCTTTCCGGCAGAAGCAGTCCTCCCAGCGGCTCTCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTa                           <  2:1496133/138‑1 (MQ=255)
                                                                                                            aaaCGCTATTGGCAAAACTGTCGCTTTACTTCTGGTCGCGTGAGATAGCGCGCCAGACGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATACCGGTCATTAAGGCATAGCCGCGTTTCTGCGCTTCTGCCACGGTa                           <  2:738606/140‑1 (MQ=255)
                                                                                                            aaaCGCTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTa                           <  1:2770094/140‑1 (MQ=255)
                                                                                                            aaaCGCAATTGGCAAAACTGTCGCGTTCGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGGCCCAGTGGATATCGGTCATTCAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGTTa                           <  2:1548816/140‑1 (MQ=255)
                                                                                                                 cTATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGAcc                      <  2:2616668/140‑1 (MQ=255)
                                                                                                                  tATTGGCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGAccc                     >  1:123324/1‑140 (MQ=255)
                                                                                                                      ggCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg                 <  1:3941781/140‑1 (MQ=255)
                                                                                                                      ggCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg                 <  1:648748/140‑1 (MQ=255)
                                                                                                                      ggCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg                 <  1:4392614/140‑1 (MQ=255)
                                                                                                                      ggCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAgcg                 <  2:2767052/140‑1 (MQ=255)
                                                                                                                       gCAAAACTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTGTTGCCAGTGGATATCGGTCAGTAAGGCAGAGGCGCGTTTCTGCGCTTCTGCCGCGGTATGACGCAccgc                >  2:127885/1‑140 (MQ=255)
                                                                                                                        cAAAACTGTCGCGTGAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGAAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGcgcg               <  1:604719/140‑1 (MQ=255)
                                                                                                                         aaaaCTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCgg              >  1:2273380/1‑140 (MQ=255)
                                                                                                                         aaaaCTGTCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGGTGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCgg              >  1:1744449/1‑140 (MQ=255)
                                                                                                                                tCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  2:3118388/140‑1 (MQ=255)
                                                                                                                                tCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  1:1659750/140‑1 (MQ=255)
                                                                                                                                tCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  1:1906580/140‑1 (MQ=255)
                                                                                                                                tCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  1:2348127/140‑1 (MQ=255)
                                                                                                                                tCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGGATGACCCAGCTCGGTGACGca       >  2:3878708/1‑140 (MQ=255)
                                                                                                                                tCGCGTTAGTTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCAGCAGAAGCAGTAGTCCAAGTGGATATCGGTCATTAAGGCATAGGCGCGCTTCTGCGCTTCTGCCACAGTATGACCCAGCGCGGTGACGca       >  2:3650133/1‑140 (MQ=255)
                                                                                                                                tCGCGTTAGTTCTGCTCGCGTTCGATAGCCCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  2:3580331/140‑1 (MQ=255)
                                                                                                                                tCGCATTAGTCCTGCTAGCGTTCGATACCGCGCCCGCCGATATCTTCCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGca       <  2:1601485/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                        
AGGATATTCAGCCGTTGGTGGAAGTGGAAAAAGAGGTGATTCTGGCGGCGCTGGAGAAAACGGGCGGCAACAAAACCGAAGCCGCCCGTCAGTTAGGGATCACGCGCAAAACGCTATTGGCAAAACTGTCGCGTTAATTCTGCTCGCGTTCGATAGCGCGCCAGCCGATATCTTTCCGGCAGAAGCAGTCGTCCCAGTGGATATCGGTCATTAAGGCATAGGCGCGTTTCTGCGCTTCTGCCACGGTATGACCCAGCGCGGTGACGCACAGTA  >  NZ_CP025268/4206431‑4206703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑