BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,333,560	T→C	V378A (GTG→GCG)	proP →	glycine betaine/L‑proline transporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,333,560	0	T	C	100.0%	922.9 / NA	277	V378A (GTG→GCG)	proP	glycine betaine/L‑proline transporter ProP
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (142/135); total (142/135)

Only 100 of 277 total aligned reads displayed.

TGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTAC  >  NZ_CP025268/4333423‑4333696

  ttGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATg                                                                                                                                      >  2:104111/1‑140 (MQ=255)

  ttGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATg                                                                                                                                      >  1:1630726/1‑140 (MQ=255)

  ttGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATg                                                                                                                                      >  1:4451480/1‑140 (MQ=255)

  ttGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATg                                                                                                                                      <  1:2193866/140‑1 (MQ=255)

   tGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGt                                                                                                                                     >  1:654328/1‑140 (MQ=255)

     cccTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTGACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTc                                                                                                                                   >  2:3961861/1‑140 (MQ=255)

     cccTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGGAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTc                                                                                                                                   >  2:4727868/1‑140 (MQ=255)

         gTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGa                                                                                                                               <  1:889539/140‑1 (MQ=255)

         gTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGa                                                                                                                               <  1:4687536/140‑1 (MQ=255)

          tttGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGAc                                                                                                                              >  2:945965/1‑140 (MQ=255)

          tttGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGAc                                                                                                                              >  2:533661/1‑140 (MQ=255)

          tttGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGAc                                                                                                                              >  2:2139507/1‑140 (MQ=255)

            tgtgTTGGCGATCGGGGCGTTTATTCTGATTAACAGTAACGCCATCCGCGTGATTTTTGAAGGGCTACTGATGCTGGCGGTGATCCTTCACTGCTTTACGGGCGTTATGCCTTCTATCTTGCCAGCGATGTTCCcgccgc                                                                                                                            <  1:3054860/140‑1 (MQ=255)

            tgtgTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTCTGGCTTCTACCTTGCCAGCGATGTTCCCGACGc                                                                                                                            <  2:1081202/140‑1 (MQ=255)

            tgtgTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGc                                                                                                                            <  2:1011743/140‑1 (MQ=255)

            tgtgTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGc                                                                                                                            <  2:4447176/140‑1 (MQ=255)

            tgtgTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGc                                                                                                                            <  2:2964268/140‑1 (MQ=255)

              tgtTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCat                                                                                                                          <  1:2276374/140‑1 (MQ=255)

                    tgATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGCTTTTTGCCGGGTTACTGACGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGt                                                                                                                    >  2:1353675/2‑140 (MQ=255)

                    cGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGAGTTTTGCCGGGTTACTGATGCTGGCGGCGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTCCCTTCCCAGCGATGTTCCCGACGCATATCCgc                                                                                                                    >  2:3313796/1‑139 (MQ=255)

                        cccGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACa                                                                                                                >  1:4887051/1‑140 (MQ=255)

                             cGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAgcgcg                                                                                                           >  1:1876294/1‑140 (MQ=255)

                                   ttCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcg                                                                                                     <  1:764969/140‑1 (MQ=255)

                                      tGATTAACAGTTGCGTCATCGGCCTGATTTTTGTCGGGTTACTGCTGCTGTCTGTGCTCCGTAACTGATTTACGGGCGTTCCGGTTTCTACCTTGCCAGCGATGTTTCCGACGCTTTTCCTTTACAGCGCGCTggcggcg                                                                                                  <  2:1263726/140‑1 (MQ=255)

                                      tGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                                  <  1:3621404/140‑1 (MQ=255)

                                      tGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTACCGACGCATATCCGTTACAGCGCGCTggcggcg                                                                                                  <  2:107266/140‑1 (MQ=255)

                                       gATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcgg                                                                                                 >  1:4747774/1‑140 (MQ=255)

                                       gATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcgg                                                                                                 >  1:1285458/1‑140 (MQ=255)

                                       gATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcgg                                                                                                 >  1:1116288/1‑140 (MQ=255)

                                        aTTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTggcggcggc                                                                                                >  1:4480836/1‑140 (MQ=255)

                                         ttAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCa                                                                                               <  1:3519089/140‑1 (MQ=255)

                                           aaCAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCAtt                                                                                             <  2:1544727/140‑1 (MQ=255)

                                           aaCAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCAtt                                                                                             <  1:2578581/140‑1 (MQ=255)

                                                tAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTccc                                                                                                                                 <  2:4661139/99‑1 (MQ=255)

                                                tAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTccc                                                                                                                                 >  1:4661139/1‑99 (MQ=255)

                                                tAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAata                                                                                        >  1:3988719/1‑140 (MQ=255)

                                                tAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAata                                                                                        >  2:165882/1‑140 (MQ=255)

                                                tAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAata                                                                                        >  1:4699525/1‑140 (MQ=255)

                                                  aCGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATAtt                                                                                      >  1:1930362/1‑140 (MQ=255)

                                                  aCGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATAtt                                                                                      >  1:599114/1‑140 (MQ=255)

                                                  aCGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATAtt                                                                                      >  1:4242478/1‑140 (MQ=255)

                                                   cGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCCACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATAttt                                                                                     <  2:4646044/140‑1 (MQ=255)

                                                       aTCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCAGTTAATATTTCGGt                                                                                 >  2:4359444/1‑140 (MQ=255)

                                                        tCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTg                                                                                >  2:2318831/1‑140 (MQ=255)

                                                        tCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTg                                                                                >  2:1823360/1‑140 (MQ=255)

                                                          ggCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCt                                                                              >  1:713939/1‑140 (MQ=255)

                                                                ttttttGCCGCGTTAGTGCTGCTGGCGGTGACCGTTCACTGCTTTACGGCCGTTATGGCTTCTACCTTGCCAGCAATGTCGCCGACGCATATCCGTTCCAGCGCGCTGGCGGCGGCATTTACTATTTCGGTGCTGGTTGc                                                                        <  2:4242247/139‑1 (MQ=255)

                                                                aTTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGc                                                                        >  1:146709/1‑140 (MQ=255)

                                                                aTTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGc                                                                        <  1:2983762/140‑1 (MQ=255)

                                                                aTTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGc                                                                        <  1:3334613/140‑1 (MQ=255)

                                                                     tGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTc                                                                   >  1:1330687/1‑140 (MQ=255)

                                                                      gCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCt                                                                  <  1:623743/140‑1 (MQ=255)

                                                                           gTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGc                                                             <  2:718567/140‑1 (MQ=255)

                                                                            ttACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGcc                                                            <  1:4052057/140‑1 (MQ=255)

                                                                            atACAGATGCTGGTTGTGATCCTTAACGGCCTTCCAGATGTTCTGCCTCCTACCTTGCCAGCCATCGTCCCGAGGCATATCGGTTCCGCCGCGCTGGCGGCGGCATTTGATATTTCGGTGCTCGTTGCCGGTCTGACGcc                                                            <  2:1427742/139‑1 (MQ=255)

                                                                              attGATGCTGGCGGTGATCCTGACCTGCTTTACGCGCGTTATGGCTTCTACCTTGCCAGCGATGTGCACGACGCATATCCGTTGCAGCGCGCTGGCGGCGGCATTGAAGATTTCGGTGCTGGTTGCCGGTCTGACGCCaa                                                          <  2:3327410/138‑1 (MQ=255)

                                                                                    gCTGGCGGTGATCCTTAACTGCTTTACAGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTgg                                                    >  2:3742301/1‑140 (MQ=255)

                                                                                     cTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTggc                                                   >  1:1545700/1‑140 (MQ=255)

                                                                                         cGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCTAACGCTGGCGGcc                                               >  2:4435297/1‑140 (MQ=255)

                                                                                         cGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGcc                                               >  1:2745049/1‑140 (MQ=255)

                                                                                            tGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCctgg                                            <  2:4189589/140‑1 (MQ=255)

                                                                                             gATACTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCctggc                                           <  2:3884912/140‑1 (MQ=255)

                                                                                                  ttAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTc                                      >  1:1567277/1‑140 (MQ=255)

                                                                                                    aaCTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGTTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGACTAACGCCAACGCTGGCGGCCTGGCTGCTCGa                                    >  2:2784880/1‑140 (MQ=255)

                                                                                                       tGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAg                                 <  1:2429728/140‑1 (MQ=255)

                                                                                                        gCTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGc                                >  1:3679416/1‑140 (MQ=255)

                                                                                                         cTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                               >  1:412446/1‑140 (MQ=255)

                                                                                                         cTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                               >  1:1988144/1‑140 (MQ=255)

                                                                                                         cTTTACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCt                               >  1:3155399/1‑140 (MQ=255)

                                                                                                          tttACGGGAGTTCTGGCTTGTATCTTGCCAGCGTTGTTGCCGTATCATACTCGTTACACCGCGGTGGCGGCGGCATTTAATGGTTCGGTGCTCCTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCGGGTCGAAAGCTc                              >  2:3598795/1‑140 (MQ=255)

                                                                                                           ttACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCg                             >  2:4797947/1‑140 (MQ=255)

                                                                                                            tACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGc                            >  2:1583265/1‑140 (MQ=255)

                                                                                                            tACGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGc                            <  1:5040018/140‑1 (MQ=255)

                                                                                                             aCGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCa                           <  1:3779690/140‑1 (MQ=255)

                                                                                                             aCGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCa                           <  1:496400/140‑1 (MQ=255)

                                                                                                             aCGGGCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCa                           <  1:4841534/140‑1 (MQ=255)

                                                                                                                 gCGTTATGGCTTCTACTTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAAt                       <  1:2820943/140‑1 (MQ=255)

                                                                                                                 gCGTTATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAAt                       >  2:4540601/1‑140 (MQ=255)

                                                                                                                    ttATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCtg                    >  1:3842450/1‑140 (MQ=255)

                                                                                                                     tATGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCtga                   <  1:4809816/140‑1 (MQ=255)

                                                                                                                      aTGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACCGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCCACGCTGGCCGCCTGGCTGGTCGAAAGCTCGCAGAATCtgat                  <  2:2722746/140‑1 (MQ=255)

                                                                                                                      aTGGCTTCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGAGTGGTTGAAAGCTCGCAGAATCtgat                  >  2:4062932/1‑140 (MQ=255)

                                                                                                                          cTTCTA‑CTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgc             <  1:1388682/140‑1 (MQ=255)

                                                                                                                           ttCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCtgctaaagc             >  2:315129/1‑133 (MQ=255)

                                                                                                                           ttCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgc             <  1:2712056/140‑1 (MQ=255)

                                                                                                                            tCTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAtgcc            <  2:2206113/140‑1 (MQ=255)

                                                                                                                             cTACCTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTAACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAAACTGATGAcgccc           >  2:2071030/1‑139 (MQ=255)

                                                                                                                                 cTTGCCGGCGATGTTCCCGACGCATCTCCGTTGCAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATgcctgcct       <  2:3691316/140‑1 (MQ=255)

                                                                                                                                 cTTGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGAATCTAATGTATCGGTGCTGGTTGCCGGTCTGACACCAACACCGGCCGCCTGGCTGGTCGAAAGCCCGCAGACTCTGATCCtgccttccg       >  2:840169/1‑139 (MQ=255)

                                                                                                                                   tGCCAGCGATGTTCCCGACGGATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAt     >  2:3530178/1‑140 (MQ=255)

                                                                                                                                   tGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATCTTTCGCTGCTCGTTGCCGGTCTGCCGCCATCGATGGCGGCCTGGCTGGTCGAATGCTCGCAGAATCTCATGCTGCCTGCCTAt     <  2:4956252/140‑1 (MQ=255)

                                                                                                                                   tGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAt     <  2:567228/140‑1 (MQ=255)

                                                                                                                                   tGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAt     >  1:1712778/1‑140 (MQ=255)

                                                                                                                                   tGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAt     >  1:1027229/1‑140 (MQ=255)

                                                                                                                                   tGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAt     <  1:2896325/140‑1 (MQ=255)

                                                                                                                                   tGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAt     <  1:4262749/140‑1 (MQ=255)

                                                                                                                                   tGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGAGGCCTGCCTAt     >  2:3745284/1‑140 (MQ=255)

                                                                                                                                   tGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAAGCTGATGATGCCTGCCTAt     >  1:519545/1‑140 (MQ=255)

                                                                                                                                   tGCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCGCGCAGAATCTGATGATGCCTGCCTAt     <  1:3130661/140‑1 (MQ=255)

                                                                                                                                    gCCAGCGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTAtt    >  2:5009241/1‑140 (MQ=255)

TGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACGTCATCGGCCTGATTTTTGCCGGGTTACTGATGCTGGCGGTGATCCTTAACTGCTTTACGGGCGTTATGGCTTCTACCTTGCCAGTGATGTTCCCGACGCATATCCGTTACAGCGCGCTGGCGGCGGCATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTAC  >  NZ_CP025268/4333423‑4333696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`