BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,424,719	T→C	V203A (GTC→GCC)	ulaD →	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,424,719	0	T	C	100.0%	654.7 / NA	198	V203A (GTC→GCC)	ulaD	3‑keto‑L‑gulonate‑6‑phosphate decarboxylase UlaD
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (94/104); total (94/104)

Only 100 of 198 total aligned reads displayed.

TCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGC  >  NZ_CP025268/4424585‑4424854

  tttCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGt                                                                                                                                  <  1:3252957/140‑1 (MQ=255)

  tttCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGt                                                                                                                                  >  2:1727002/1‑140 (MQ=255)

  tttCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGt                                                                                                                                  <  1:4815060/140‑1 (MQ=255)

  tctCCGATAGGGGCGTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGt                                                                                                                                  <  2:2805962/138‑1 (MQ=255)

   ttCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTc                                                                                                                                 >  1:3689754/1‑140 (MQ=255)

         tatGGGCTTCAAAGTCACCGCCCCCCGCCGCCTGCCGCTGGAATTTCTGCCGCTGTTCAAGGGTATCCGGCTTCGCGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCGTCTCCGGTGGAAGCCGCACGTCAGTTCa                                                                                                                           <  2:3442943/140‑1 (MQ=255)

           ggggCTTCAAGGTCACCGTCGCCGGAGGCCTGGCGCTGGAAGTTCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCaaa                                                                                                                         <  2:1481052/139‑1 (MQ=255)

                ttCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTc                                                                                                                    <  2:1325175/140‑1 (MQ=255)

                ttCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTc                                                                                                                    <  1:4017902/140‑1 (MQ=255)

                ttCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTc                                                                                                                    <  1:748185/140‑1 (MQ=255)

                gtCAGAGTCGGCGACACCGGAGCCCTGGCGCTGGAAAATCTGCCGCCGTTCAGGGGTACACCGACTCCCGTCCTGATCGCGGGCCGCAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTc                                                                                                                    <  2:3303559/139‑1 (MQ=255)

                     aGTCACCGTCACCGGAGGCCTGGCGCTGGCAGATCTGCCGATGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGGGGAAGCCGCACGTCAGTTCAAACGTTCCATCg                                                                                                               <  1:339788/140‑1 (MQ=255)

                     aGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCg                                                                                                               <  1:94086/140‑1 (MQ=255)

                        ggccgtcagagGACGCCTGGGGCTGGAAGATCTGCCGCTGTTGTTGGGTTTTCCGATTCACGTCTTTAGCGCGGGCCGTAGTGGCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTg                                                                                                            <  2:300014/138‑1 (MQ=255)

                        caccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTg                                                                                                            >  2:82674/1‑140 (MQ=255)

                        caccgtcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTg                                                                                                            <  1:2975216/140‑1 (MQ=255)

                          ccgtcaccgGGGGCCGGGCGCTGAACGCCCTGCCGATGTTCATGGGCATTCCGATTCACGTCTTTACCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGaa                                                                                                          <  2:2163788/140‑1 (MQ=255)

                             tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                       <  1:4542840/140‑1 (MQ=255)

                             tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                       <  1:4190489/140‑1 (MQ=255)

                             tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                       <  1:1954172/140‑1 (MQ=255)

                             tcaccgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                       <  1:2633349/140‑1 (MQ=255)

                             tcaccgGAGGCCGGGAGGTGGAAGACCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTGTCGCGGGCCGTAGTATCCGGGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAGCtg                                                                                                       <  2:3909697/140‑1 (MQ=255)

                             gcgccgGGGGCGTGGAGGTGGAAGACCCGCCGCTGTTCAAGGTTACTCCTATTCACGTCTTTGTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACtg                                                                                                       <  2:3520385/137‑1 (MQ=255)

                                 cgGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTggg                                                                                                   >  1:2837974/1‑140 (MQ=255)

                                     ggCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTa                                                                                               >  1:549939/1‑140 (MQ=255)

                                       ccTGGCGGTGGAAGATCTGCCGCTGTTCAAGGGTAGTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGGTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg                                                                                             <  1:4801137/140‑1 (MQ=255)

                                        cTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                            >  1:1610523/1‑140 (MQ=255)

                                        cTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                            <  2:203981/140‑1 (MQ=255)

                                        cTGGCGCCGGGAGAGCTGCCGCCGCTCACGGGTATTCCCGTTCACCCCTTTAGCGCGGGCCGGAGTCCCCCTGATGCCGCTTCGCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAgg                                                                                            <  2:2414203/140‑1 (MQ=255)

                                         tGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTAACCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCGAACGTTCCATCGCTGAACTGTGGGGCTAAGGa                                                                                           >  2:4221371/1‑140 (MQ=255)

                                                ggAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGata                                                                                    >  2:45252/1‑140 (MQ=255)

                                                ggAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGata                                                                                    >  1:1929851/1‑140 (MQ=255)

                                                 gAAGATCTGCCGCTGTTCAAGGGTCTTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGatat                                                                                   <  1:1105139/140‑1 (MQ=255)

                                                  aaGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATAtg                                                                                  <  1:603480/140‑1 (MQ=255)

                                                  aaGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTAAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATAtg                                                                                  <  1:4022737/140‑1 (MQ=255)

                                                       gtGCCGCTGTTCAAGGGTATTCCGATTCCCGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTc                                                                             <  2:4393716/139‑1 (MQ=255)

                                                       cTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTc                                                                             <  1:3961806/140‑1 (MQ=255)

                                                       cTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGGAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTc                                                                             <  1:1917961/140‑1 (MQ=255)

                                                       cTGCCGCTGTTCAAGGGTATCCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTc                                                                             <  1:1707137/140‑1 (MQ=255)

                                                           cGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCcaaa                                                                         <  1:5327/140‑1 (MQ=255)

                                                                ttCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGTCGCTTCTCCGGCGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCGGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAAt                                                                    >  2:3650168/1‑140 (MQ=255)

                                                                ttCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAAt                                                                    >  1:919157/1‑140 (MQ=255)

                                                                 tCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATc                                                                   >  1:696759/1‑140 (MQ=255)

                                                                       gTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCtt                                                             >  2:2933280/1‑140 (MQ=255)

                                                                        tATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTg                                                            <  2:579338/140‑1 (MQ=255)

                                                                          ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGCTGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                          >  1:1452743/1‑140 (MQ=255)

                                                                          ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGCTATGTTGTCCAAACAAATCCCGCTTGGc                                                          >  1:4405707/1‑140 (MQ=255)

                                                                          ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                          >  1:1565649/1‑140 (MQ=255)

                                                                          ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                          >  1:2545253/1‑140 (MQ=255)

                                                                          ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                          >  1:1759670/1‑140 (MQ=255)

                                                                          ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                          >  1:1204815/1‑140 (MQ=255)

                                                                          ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                          >  2:3224785/1‑140 (MQ=255)

                                                                          ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                          >  1:1023248/1‑140 (MQ=255)

                                                                          ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                          >  2:4893668/1‑140 (MQ=255)

                                                                          ttCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGc                                                          >  1:3787577/1‑140 (MQ=255)

                                                                            ccGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCAt                                                        <  1:4664654/140‑1 (MQ=255)

                                                                             cGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATc                                                       <  1:4431504/140‑1 (MQ=255)

                                                                               aTTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTa                                                     >  2:2720890/1‑140 (MQ=255)

                                                                                ttCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTAt                                                    >  2:2435887/1‑140 (MQ=255)

                                                                                         ttATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAgcg                                           >  1:5044264/1‑140 (MQ=255)

                                                                                           ggcgggggCGGTAGGACCCGGGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCCTCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt                                         <  2:4883267/135‑1 (MQ=255)

                                                                                           aTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg                                                                                             <  2:1085966/88‑1 (MQ=255)

                                                                                           aTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAg                                                                                             >  1:1085966/1‑88 (MQ=255)

                                                                                           aTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt                                         <  1:4286465/140‑1 (MQ=255)

                                                                                           aTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt                                         <  2:1565649/140‑1 (MQ=255)

                                                                                           aTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt                                         <  1:1767090/140‑1 (MQ=255)

                                                                                           aTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt                                         <  1:2423535/140‑1 (MQ=255)

                                                                                           aTCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGATGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCt                                         <  1:1851434/140‑1 (MQ=255)

                                                                                             cgcgGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTcc                                       <  1:631575/140‑1 (MQ=255)

                                                                                             cgcgGGCCGTAGGATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTcc                                       >  2:5049517/1‑140 (MQ=255)

                                                                                               cgGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTcccc                                     >  1:3681525/1‑140 (MQ=255)

                                                                                                gggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccg                                    <  1:1308025/140‑1 (MQ=255)

                                                                                                 ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCACACAAATCCTGCTTGGCATCTATGAAAAAGCGCTCCccgc                                   >  1:1870565/1‑140 (MQ=255)

                                                                                                 ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccgc                                   >  1:3321678/1‑140 (MQ=255)

                                                                                                 ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccgc                                   >  2:4359579/1‑140 (MQ=255)

                                                                                                 ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccgc                                   >  2:3918976/1‑140 (MQ=255)

                                                                                                 ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGGTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGTGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAATCTCTCCccgc                                   >  2:3745951/1‑140 (MQ=255)

                                                                                                 ggCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGGTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAACGCGCTCCccgc                                   >  2:2360616/1‑140 (MQ=255)

                                                                                                  gCCGGAGGAGCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGCCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCccgcc                                  <  2:456463/140‑1 (MQ=255)

                                                                                                    cGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCgg                                >  2:2557666/1‑140 (MQ=255)

                                                                                                     gtagtaTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCggg                               <  1:2348319/140‑1 (MQ=255)

                                                                                                       agtaTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGa                             <  1:2667696/140‑1 (MQ=255)

                                                                                                        gtaTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAg                            <  1:1024152/140‑1 (MQ=255)

                                                                                                         taTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGt                           <  1:808624/140‑1 (MQ=255)

                                                                                                          aTCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTg                          <  1:633351/140‑1 (MQ=255)

                                                                                                           tCCGTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCACACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgc                         >  1:2060959/1‑140 (MQ=255)

                                                                                                              gTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTTTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCGATGAAAAAGCGCTCCCCGCCGGGGAGTgctgg                      >  1:527283/1‑140 (MQ=255)

                                                                                                              gTGATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctgg                      >  1:2582708/1‑140 (MQ=255)

                                                                                                                gATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTTTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCACCGGGGAGTgctggct                    >  1:2820548/1‑140 (MQ=255)

                                                                                                                gATGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggct                    >  1:3132144/1‑140 (MQ=255)

                                                                                                                 aTGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctggctg                   >  1:3020597/1‑140 (MQ=255)

                                                                                                                 aTGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctg                       >  2:3658023/1‑136 (MQ=255)

                                                                                                                 aTGCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTgctg                       <  1:3657922/136‑1 (MQ=255)

                                                                                                                   ggcGCTTCTCCGGTGGAGGCCGCACGTCAGGTCAAAAGTTCCATCGCTGAACGGGGGGGCTAAGGAGCGGATAGGTTGTCCAAAAAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggcgggc                 <  2:728508/138‑2 (MQ=255)

                                                                                                                   gCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCAATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGa                 >  2:1601261/1‑140 (MQ=255)

                                                                                                                   gCCGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGGGCGGGTCAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTACGACAAAGCGCTCCCCGCCGGGGCGTGCTATCTGGa                 >  2:2329512/1‑140 (MQ=255)

                                                                                                                   gCCGCGTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCTTCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAAGCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGctggctggc                 <  2:1228507/140‑2 (MQ=255)

                                                                                                                    ccGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGaa                >  1:2300301/1‑140 (MQ=255)

                                                                                                                     cGCTTCTCCGGTGGAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAAc               >  2:540081/1‑140 (MQ=255)

                                                                                                                                  gAAGCCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGc  >  1:1618631/1‑140 (MQ=255)

TCTTTCCGATATGGGCTTCAAAGTCACCGTCACCGGAGGCCTGGCGCTGGAAGATCTGCCGCTGTTCAAGGGTATTCCGATTCACGTCTTTATCGCGGGCCGTAGTATCCGTGATGCCGCTTCTCCGGTGGAAGTCGCACGTCAGTTCAAACGTTCCATCGCTGAACTGTGGGGCTAAGGAGCGGATATGTTGTCCAAACAAATCCCGCTTGGCATCTATGAAAAAGCGCTCCCCGCCGGGGAGTGCTGGCTGGAACGCCTGCAACTGGC  >  NZ_CP025268/4424585‑4424854

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`