BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,430,004	T→C	pseudogene (386/402 nt) 213 (TAA→TAG)	CXP41_RS22940 → ytfB ←	TetR/AcrR family transcriptional regulator cell division protein YtfB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,430,004	0	T	C	99.6%	937.7 / NA	279	pseudogene (386/402 nt) 213 (TAA→TAG)	CXP41_RS22940 ytfB	TetR/AcrR family transcriptional regulator cell division protein YtfB
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (190/88); minor base A (1/0); total (191/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 279 total aligned reads displayed.

GGCGGATATTATTTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAA  >  NZ_CP025268/4429868‑4430139

ggCGGATATTATTTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTAc                                                                                                                                      >  1:2725103/1‑140 (MQ=255)

     atatTATTTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCgcgc                                                                                                                                 <  2:2615183/140‑1 (MQ=255)

     atatTATTTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCgcgc                                                                                                                                 <  2:4957105/140‑1 (MQ=255)

       attattTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCAc                                                                                                                               >  2:7512/1‑140 (MQ=255)

       attattTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCAc                                                                                                                               >  1:348601/1‑140 (MQ=255)

       attattTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCAc                                                                                                                               >  1:233759/1‑140 (MQ=255)

               aTCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGAGCACGAATaaaa                                                                                                                       >  2:3596723/1‑140 (MQ=255)

                 cAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACt                                                                                                                     <  2:3316165/140‑1 (MQ=255)

                   aCCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGc                                                                                                                   >  1:3918332/1‑140 (MQ=255)

                              cTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGGTGGATTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTgg                                                                                                        >  1:3637819/1‑140 (MQ=255)

                               ttGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGc                                                                                                       >  1:58703/1‑140 (MQ=255)

                                 ggCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                                     <  1:3835613/140‑1 (MQ=255)

                                 ggCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                                     >  1:3129141/1‑140 (MQ=255)

                                 ggCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                                     <  2:3229280/140‑1 (MQ=255)

                                 ggCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                                     >  1:193056/1‑140 (MQ=255)

                                 ggCCTGGTTTATCACATCAGTATGGTGTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTCGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCgg                                                                                                     <  2:4010472/140‑1 (MQ=255)

                                  ggctagTTTATCACATCAGTAAGGTTTCATCCCAGGCAATGCAAGCCAGACGTAAGGTCATCAAAGATACAATCCTTAGCTGGCGTTTGGTTGACCCTTCATCTACCGCGCACGAATAAATCTGCCATCCGGCTGGCggg                                                                                                    <  2:596396/135‑1 (MQ=255)

                                  gCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGCCGTAAGGTGATCAACGAGACCATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCGATCCGGCTTGCggg                                                                                                    >  2:2178097/1‑140 (MQ=255)

                                  gCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCggg                                                                                                    >  1:685984/1‑140 (MQ=255)

                                  gCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCggg                                                                                                    >  2:1789022/1‑140 (MQ=255)

                                  gCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCggg                                                                                                    >  2:2519039/1‑140 (MQ=255)

                                  gCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCggg                                                                                                    >  1:3263845/1‑140 (MQ=255)

                                  gCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCggg                                                                                                    >  1:3526699/1‑140 (MQ=255)

                                       gTTTATCACATCAGTATGGTTTCACTCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGATCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGAGTGAAc                                                                                               >  2:2997482/1‑140 (MQ=255)

                                       gTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAAc                                                                                               <  1:4719444/140‑1 (MQ=255)

                                       gTTTATCACATCAGTATGGGTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGTGACAATCCTTATCTGGCTTTTGGTTGACCCTTCATCTACCACGCACGAATAAAACTGCCATCCGTCGTGCGTGTGAAc                                                                                               >  2:4253694/1‑140 (MQ=255)

                                         ttATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAg                                                                                             <  1:4605348/140‑1 (MQ=255)

                                            tCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGAc                                                                                          <  1:2409265/140‑1 (MQ=255)

                                            tCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGAc                                                                                          <  1:1899927/140‑1 (MQ=255)

                                            tCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGAc                                                                                          <  1:1499950/140‑1 (MQ=255)

                                              acaTCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCt                                                                                        <  1:2701825/140‑1 (MQ=255)

                                              acaTCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCt                                                                                        <  1:1380478/140‑1 (MQ=255)

                                                ctCAGTATGGTTTCCCCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATACAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGt                                                                                      <  2:4127048/139‑1 (MQ=255)

                                                 tCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGtt                                                                                     >  1:3306836/1‑140 (MQ=255)

                                                 tCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGtt                                                                                     >  1:4099323/1‑140 (MQ=255)

                                                 tCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGtt                                                                                     >  1:1744119/1‑140 (MQ=255)

                                                          tttCACCCCAGGCACTGTAAGCCAGACGTAAGGTCATCAAAGAGACAATCCATAGCTGGCTGTTGGTTGACGCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTcc                                                                            >  2:36876/1‑140 (MQ=255)

                                                          tttCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTcc                                                                            >  2:2234345/1‑140 (MQ=255)

                                                          tttCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTcc                                                                            >  1:109556/1‑140 (MQ=255)

                                                          tttCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTcc                                                                            >  1:1639140/1‑140 (MQ=255)

                                                          tttCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTcc                                                                            >  1:1787626/1‑140 (MQ=255)

                                                           ttCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTccc                                                                           <  2:3366905/140‑1 (MQ=255)

                                                           ttCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTccc                                                                           <  2:536906/140‑1 (MQ=255)

                                                           ttCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTccc                                                                           <  2:3902980/140‑1 (MQ=255)

                                                           ttCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTAATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTccc                                                                           <  1:1703957/140‑1 (MQ=255)

                                                            tCACCCCAGGCATTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTGTAGGTTGACCCTCCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGCACAGGACCTGTTGATTATTcccc                                                                          >  2:5024185/1‑140 (MQ=255)

                                                            tCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCGTTAGCTGGCGTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTAATTATTcccc                                                                          >  2:3730653/1‑140 (MQ=255)

                                                            tCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTcccc                                                                          >  1:2880252/1‑140 (MQ=255)

                                                             cACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCg                                                                         <  2:4316972/140‑1 (MQ=255)

                                                              aCCCCAGGCACTGGCAGCCACACGTAAGGTCATCACAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCGCGACTAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTGGATTATTCCCCGt                                                                        >  2:1205268/1‑140 (MQ=255)

                                                               ccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTATCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTa                                                                       >  1:2976141/1‑140 (MQ=255)

                                                               ccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTa                                                                       >  2:3406982/1‑140 (MQ=255)

                                                               ccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTa                                                                       >  2:3215353/1‑140 (MQ=255)

                                                               ccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTa                                                                       >  2:363089/1‑140 (MQ=255)

                                                               ccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTa                                                                       >  2:1683595/1‑140 (MQ=255)

                                                               ccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTa                                                                       >  1:3712782/1‑140 (MQ=255)

                                                               ccccAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTa                                                                       >  1:4962433/1‑140 (MQ=255)

                                                               ccccAGGCACTGGAAGCCAGACGTAAGGGCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTa                                                                       >  1:1288602/1‑140 (MQ=255)

                                                                  cAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCa                                                                    <  1:921210/140‑1 (MQ=255)

                                                                   agacACTAGAATCCAGTCGTAAGACAATTAAACATACAATACTTAGCTGGCTTTTGGTTCACCCTTCAACTACCACGCACAAATAAAACTGCCATCCGGCTAGCAGGTGAAAAGGACCTGATGAGTATTCCCCGTATCaa                                                                   <  2:347030/137‑1 (MQ=255)

                                                                   aGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCaa                                                                   >  1:5063874/1‑140 (MQ=255)

                                                                   aGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTATGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCaa                                                                   >  2:2837904/1‑140 (MQ=255)

                                                                    ggCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAAt                                                                  >  2:3868604/1‑140 (MQ=255)

                                                                    ggCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAAt                                                                  >  2:2152657/1‑140 (MQ=255)

                                                                    ggCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTGTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCa                                                                    <  1:1145147/138‑1 (MQ=255)

                                                                    ggCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCAATTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCAGCCGCCTGGAGGGTGAACAGGACCTGTTTATTATTCCCCGTATCAAt                                                                  >  2:1896828/1‑140 (MQ=255)

                                                                        cTGGACGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGtt                                                              <  2:4759633/140‑1 (MQ=255)

                                                                        cTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGtt                                                              <  2:58703/140‑1 (MQ=255)

                                                                         tGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTa                                                             >  1:4909833/1‑140 (MQ=255)

                                                                            aaGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGACTAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGc                                                          >  1:1403628/1‑140 (MQ=255)

                                                                             aGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGcc                                                         <  2:1438038/140‑1 (MQ=255)

                                                                               ccAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCg                                                       >  2:154318/1‑140 (MQ=255)

                                                                                             atcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTg                                         >  1:4175856/1‑140 (MQ=255)

                                                                                             atcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTg                                         >  1:1023948/1‑140 (MQ=255)

                                                                                             atcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTg                                         >  2:1762636/1‑140 (MQ=255)

                                                                                             atcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTg                                         >  1:4275209/1‑140 (MQ=255)

                                                                                             atcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGCGTGAACAGGACCCGTTGATTATTCCCCGTAACTATGGTTAAGCCCGTCCCCACTCCGCtc                                         >  2:1318988/1‑139 (MQ=255)

                                                                                              tcaAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCt                                                                                                          <  2:1128034/74‑1 (MQ=255)

                                                                                                  agagACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGtt                                    <  1:881653/140‑1 (MQ=255)

                                                                                                    agaCAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCt                                  >  1:3404832/1‑140 (MQ=255)

                                                                                                     gaCAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTg                                 >  1:3618620/1‑140 (MQ=255)

                                                                                                     gaCAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTg                                 >  1:445894/1‑140 (MQ=255)

                                                                                                      aCAATCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCAACCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTgg                                <  1:2784411/140‑1 (MQ=255)

                                                                                                         aTCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCgg                             >  2:899679/1‑140 (MQ=255)

                                                                                                         aTCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCgg                             >  1:4827090/1‑140 (MQ=255)

                                                                                                         aTCCTTAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCGGTTGATTATTCCCCGTATCAATGGTTAAGCACGTCACCACGCCGCTGGCGTTCTGGCgg                             >  2:2413038/1‑140 (MQ=255)

                                                                                                              tAGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGAtttt                        <  2:3187252/140‑1 (MQ=255)

                                                                                                               aGCTGGCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTc                       >  1:2273248/1‑140 (MQ=255)

                                                                                                                    gcgtgtGGTTGACCCTTTATCTACCGCGCACGAATAACACTGCCATCCGGTTGGCGCGTCACCCGGAGCTGCTGATGATTCACCGTACAAATGTTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCCGATTTTCACAAt                  >  2:2930224/6‑140 (MQ=255)

                                                                                                                    gCTTTTGTTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCGGGCGGGTGAACAGGACCTGTTGATTATGCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCAt                  >  2:2062787/1‑140 (MQ=255)

                                                                                                                    gCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATGCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCAt                  >  2:4436000/1‑140 (MQ=255)

                                                                                                                    gCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTGACCACGCCGCTGGCGTTCTGGCGGATTTTCACCAt                  >  2:4720009/1‑140 (MQ=255)

                                                                                                                    gCTTTTGGTTGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCAt                  >  2:3267828/1‑140 (MQ=255)

                                                                                                                             tGACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCATCTGCCCGtt         <  1:4319603/140‑1 (MQ=255)

                                                                                                                              gACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGCTTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGCTCTGGCGGATTTTCACCATCTGCCCGttt        >  2:4872526/1‑140 (MQ=255)

                                                                                                                              gACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCATCTGCCCGttt        >  1:4545256/1‑140 (MQ=255)

                                                                                                                              gACCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGCTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCATCTGCCCGttt        >  2:2321001/1‑140 (MQ=255)

                                                                                                                               aCCCTTCATCTGCCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGGGAACAGGGCCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCATCCTCCCGgttt       >  2:1074999/1‑140 (MQ=255)

                                                                                                                               aCCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCATCTGCCCGtttt       <  2:1351193/140‑1 (MQ=255)

                                                                                                                               aCCCTTCATCTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCATCTGCCCGtttt       >  1:2106463/1‑140 (MQ=255)

GGCGGATATTATTTGATCAACCAGCATACGCTTGGCCTGGTTTATCACATCAGTATGGTTTCACCCCAGGCACTGGAAGCCAGACGTAAGGTCATCAAAGAGACAATCCTTAGCTGGCTTTTGGTTGACCCTTCATTTACCGCGCACGAATAAAACTGCCATCCGGCTGGCGGGTGAACAGGACCTGTTGATTATTCCCCGTATCAATGGTTAAGCCCGTCACCACGCCGCTGGCGTTCTGGCGGATTTTCACCATCTGCCCGTTTTGCAAA  >  NZ_CP025268/4429868‑4430139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`