BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,449,815	A→G	pseudogene (3779/3779 nt)	tamB →	autotransporter assembly complex protein TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,449,815	0	A	G	99.8%	1506.9 / NA	446	pseudogene (3779/3779 nt)	tamB	autotransporter assembly complex protein TamB
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (243/202); total (243/203)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 446 total aligned reads displayed.

AGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑T‑TGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGT  >  NZ_CP025268/4449681‑4449951

    aaaTACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGa                                                                                                                                  >  2:715688/1‑140 (MQ=255)

    aaaTACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGa                                                                                                                                  >  2:1839512/1‑140 (MQ=255)

        ccGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTCGCAATGCGAatat                                                                                                                              <  2:2528752/139‑1 (MQ=255)

        aCGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAatat                                                                                                                              <  2:2225311/140‑1 (MQ=255)

            cGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGt                                                                                                                          >  1:771370/1‑140 (MQ=255)

              tGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCATAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCt                                                                                                                        >  1:3037791/1‑140 (MQ=255)

                         gACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCATAAGCCATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTa                                                                                                             <  1:2185061/140‑1 (MQ=255)

                          aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                                            >  1:2967241/1‑140 (MQ=255)

                          aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATAGCTGGAAGCCGTGTCTGGTGTAGATCATGCATTTG‑ATT‑TTCTCTATCAATTACAATTTTAGCTATGCGAATATTTGTCACCGGCAGTTTAc                                                                                                            >  2:1529429/1‑140 (MQ=255)

                           ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                                           >  1:429975/1‑140 (MQ=255)

                           ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                                           >  1:1011236/1‑140 (MQ=255)

                           ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGGAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                                           >  2:4161815/1‑140 (MQ=255)

                            tctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                                                          >  1:2005755/1‑140 (MQ=255)

                            tctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                                                          >  1:3676857/1‑140 (MQ=255)

                            tctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                                                          >  2:3134197/1‑140 (MQ=255)

                                taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                                                      <  2:3810721/140‑1 (MQ=255)

                                     acacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCacaaacaa                                                                                                 >  2:2142864/1‑140 (MQ=255)

                                      cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCGATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGATTACGCCACAAACAAg                                                                                                >  1:2838362/1‑140 (MQ=255)

                                        cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACACGGc                                                                                              >  2:3583132/1‑140 (MQ=255)

                                        cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                              >  1:2721138/1‑140 (MQ=255)

                                        cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                              >  2:2972636/1‑140 (MQ=255)

                                        cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                              >  2:3599101/1‑140 (MQ=255)

                                        cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                              >  1:1171571/1‑140 (MQ=255)

                                        cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                              >  1:3200899/1‑140 (MQ=255)

                                          cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                            <  2:749272/140‑1 (MQ=255)

                                          cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                            <  2:1670681/140‑1 (MQ=255)

                                            cgttcctttaTCGCCTCATGCCTAAACTATACCTGCCAGCCCTGTCTGGTGTAGACCACGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCACTGCCAATATTTGTCTACGGCAGTTTCCGCCACAAACAAGGCAACa                                                                                          <  2:4134953/140‑1 (MQ=255)

                                              ttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                                                        <  2:2653822/140‑1 (MQ=255)

                                              ttacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGt                                                                                        <  1:1142221/140‑1 (MQ=255)

                                               tacgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTc                                                                                       <  1:1642298/140‑1 (MQ=255)

                                                 cgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                                                     >  1:1789919/1‑140 (MQ=255)

                                                   ttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTg                                                                                   >  1:3568777/1‑140 (MQ=255)

                                                     aTCGCCTGTTGCCTAAGCTATATCTGGAAGCCGTGTATGGTGTAGACCAGGCACTGG‑A‑TATGCTCTATCAGTTCGAGGTTTAGCAAGGCGAATATGTGGCTAGGGCAGTTTACGCCGCAAACAATGCAACAGTCACTGGa                                                                                 >  2:3058733/1‑140 (MQ=255)

                                                     aTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                                                <  2:4253861/125‑1 (MQ=255)

                                                      tCGCCTGCTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGCCCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                                                <  1:1420722/140‑1 (MQ=255)

                                                      tCGCCTGCTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGC‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                                                <  1:3972373/140‑1 (MQ=255)

                                                      tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                                                <  1:3279934/140‑1 (MQ=255)

                                                       cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATg                                                                               >  1:1835755/1‑140 (MQ=255)

                                                         ccggATTCCTAAGCTATTTCTGGAAGCCGTGTCTGGTGTAGACTAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATCAc                                                                             <  2:3895960/137‑1 (MQ=255)

                                                           tGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCa                                                                           >  1:3850439/1‑140 (MQ=255)

                                                            gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                          <  2:3448488/140‑1 (MQ=255)

                                                            gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                          <  1:2532322/140‑1 (MQ=255)

                                                             aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAAt                                                                         >  1:576243/1‑140 (MQ=255)

                                                             aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTATACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAAt                                                                         >  2:678447/1‑140 (MQ=255)

                                                                ccTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                      >  1:1677426/1‑140 (MQ=255)

                                                                ccTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                      <  1:4067458/140‑1 (MQ=255)

                                                                    aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGt                                                                  >  2:5084354/1‑140 (MQ=255)

                                                                      ctctatCTGGAAGTCGTGTCTGGTGTAGACCCGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGCTGACCAATGCCCAGTTa                                                                <  2:1261583/137‑1 (MQ=255)

                                                                       tataTCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTAc                                                               <  1:3390445/140‑1 (MQ=255)

                                                                         tatCTGGAAGCCGGGTCTGGTGTAGGCCAGGCACTGG‑ATT‑TGCTCTATCAGTGGGAGGTTTAGCAATGCGAATATTTGGCTACGGCAGTTTACGCCACAAACGAGGCAACAGTCACTGGATGACCGGTGCCCAGTTACTg                                                             >  2:4421062/1‑140 (MQ=255)

                                                                           tCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTggg                                                           >  2:1410858/1‑140 (MQ=255)

                                                                            cTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAAAAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                          <  1:2299406/140‑1 (MQ=255)

                                                                            cTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTAGAGTTTTCGCAATGCGAATCTTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                          <  1:3898162/140‑1 (MQ=255)

                                                                              ggAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGa                                                        >  1:643539/1‑140 (MQ=255)

                                                                               gAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAt                                                       >  2:457953/1‑140 (MQ=255)

                                                                                aaGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAGGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAtt                                                      >  2:3953988/1‑140 (MQ=255)

                                                                                 aGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAttt                                                     >  1:1576472/1‑140 (MQ=255)

                                                                                 aGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGAttt                                                     >  2:2279230/1‑140 (MQ=255)

                                                                                   ccGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                                                   >  2:3206745/1‑140 (MQ=255)

                                                                                   ccGTGTCTGGTGTAGACCAGGCAATGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                                                   >  2:173155/1‑140 (MQ=255)

                                                                                      tgtCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTa                                                >  2:3528470/1‑140 (MQ=255)

                                                                                       gtCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTAt                                               <  1:313610/140‑1 (MQ=255)

                                                                                           ggTGTGGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTGTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGAt                                           <  2:1815749/140‑1 (MQ=255)

                                                                                           ggTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGAt                                           <  1:2105125/140‑1 (MQ=255)

                                                                                            gtgtAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGTGCGATTTCAGTATCGATa                                          >  2:4619174/1‑140 (MQ=255)

                                                                                            gtgtAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                                          <  1:4143667/140‑1 (MQ=255)

                                                                                            gtgtAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                                          >  2:4791411/1‑140 (MQ=255)

                                                                                               tAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCCCTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt                                       <  2:2812234/140‑1 (MQ=255)

                                                                                               tAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt                                       <  1:496401/140‑1 (MQ=255)

                                                                                                 gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                     <  2:1961763/140‑1 (MQ=255)

                                                                                                 gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                     <  1:857119/140‑1 (MQ=255)

                                                                                                 gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                     <  1:46959/140‑1 (MQ=255)

                                                                                                 gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                     <  1:1503523/140‑1 (MQ=255)

                                                                                                 gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAAAAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                     <  2:861603/140‑1 (MQ=255)

                                                                                                  aCCAGGCACTGGCT‑T‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAcc                                    >  2:2895677/1‑140 (MQ=255)

                                                                                                   ccAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACGAGGCAACAGTCACTGGATGACCAATGCCCAGCTACTGGGCGATTTCAGTATCGATAACTACCa                                   <  2:2712444/140‑1 (MQ=255)

                                                                                                    cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg                                  <  2:1430179/140‑1 (MQ=255)

                                                                                                    cAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAg                                  <  1:5080926/140‑1 (MQ=255)

                                                                                                      ggCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt                                >  2:3258814/1‑140 (MQ=255)

                                                                                                      ggCACTGG‑ATT‑TGCTCTATAAGTTAGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt                                >  2:4961369/1‑140 (MQ=255)

                                                                                                       gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg                               >  2:244193/1‑140 (MQ=255)

                                                                                                       gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg                               >  1:226065/1‑140 (MQ=255)

                                                                                                       gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg                               >  1:4555298/1‑140 (MQ=255)

                                                                                                        cACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGt                              <  2:377368/140‑1 (MQ=255)

                                                                                                         aCTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta                             <  1:4637550/140‑1 (MQ=255)

                                                                                                         aCTGG‑ACT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGACACAAACGAGGCAACAGTCACTGGATGGCCAATGCCCACTTACTGGGAGATTTAAGTATCGCTAACTACCAGTTGta                             <  2:1321267/140‑1 (MQ=255)

                                                                                                                tt‑tGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCt                       <  2:1089386/139‑1 (MQ=255)

                                                                                                                 t‑tGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTg                      <  1:3781150/140‑1 (MQ=255)

                                                                                                                 t‑tGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAGGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTg                      <  1:3470098/140‑1 (MQ=255)

                                                                                                                   tGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTgg                     >  1:2430584/1‑140 (MQ=255)

                                                                                                                    gCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTggg                    >  1:4491351/1‑140 (MQ=255)

                                                                                                                    gCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTggg                    >  1:4265874/1‑140 (MQ=255)

                                                                                                                       ctATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCa                 <  1:1315674/140‑1 (MQ=255)

                                                                                                                       ctATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCa                 <  1:4805643/140‑1 (MQ=255)

                                                                                                                         aTCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAcc                                                                            >  1:499988/1‑79 (MQ=255)

                                                                                                                         aTCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACt               >  2:4716068/1‑140 (MQ=255)

                                                                                                                              ttCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCa          >  2:4895447/1‑140 (MQ=255)

                                                                                                                               tCGAGTTTTAGCGATGCGAATCTTTGTCTACGGCAGTTTACGCCACAAACAGGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGGTGTAGAGCCTGGGCCACTATCCAg         >  2:1953624/1‑140 (MQ=255)

                                                                                                                                  aGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGgcg      <  1:984447/140‑1 (MQ=255)

                                                                                                                                  aGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGgcg      >  2:1165212/1‑140 (MQ=255)

AGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑T‑TGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAGT  >  NZ_CP025268/4449681‑4449951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`