BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	47,777	A→G	177 (TAA→TAG) N3S (AAC→AGC)	kefF → kefC →	glutathione‑regulated potassium‑efflux system oxidoreductase KefF glutathione‑regulated potassium‑efflux system protein KefC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	47,777	0	A	G	99.6%	736.7 / NA	226	177 (TAA→TAG) N3S (AAC→AGC)	kefF kefC	glutathione‑regulated potassium‑efflux system oxidoreductase KefF glutathione‑regulated potassium‑efflux system protein KefC
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (139/85); minor base T (1/0); total (140/86)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

CAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTG  >  NZ_CP025268/47641‑47913

cAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCa                                                                                                                                       <  1:1162525/140‑1 (MQ=255)

cAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCa                                                                                                                                       <  2:1280397/140‑1 (MQ=255)

cAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCa                                                                                                                                       >  2:1939871/1‑140 (MQ=255)

    cTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAACCCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACg                                                                                                                                   <  2:4647418/140‑1 (MQ=255)

    cTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACg                                                                                                                                   <  1:525269/140‑1 (MQ=255)

          cGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGAtt                                                                                                                             >  1:1349965/1‑140 (MQ=255)

          cGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGAtt                                                                                                                             >  1:3337562/1‑140 (MQ=255)

            ggCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCa                                                                                                                           >  2:4321398/1‑140 (MQ=255)

             gCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAg                                                                                                                          >  2:3283408/1‑140 (MQ=255)

               tGAACTGGTTGCCACCGTTTGCCATGCACTGCCCCCTTATTTGGGCCGACGAAACCCGCGAAGTGCAGGCGCGTCACCATAAGCAGGTTCTGGTGGAATGTCACGAGGCCCATCATGGGTAGCCGTACGCTTATTCTGgc                                                                                                                        >  2:1570056/1‑140 (MQ=255)

                gAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGACTGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGgcg                                                                                                                       <  2:1417730/140‑1 (MQ=255)

                gAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGgcg                                                                                                                       <  1:1960891/140‑1 (MQ=255)

                gAACTCGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGTGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGgcg                                                                                                                       <  2:4508716/140‑1 (MQ=255)

                 aaCTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGgcgc                                                                                                                      >  2:3420027/1‑140 (MQ=255)

                 aaCTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGgcgc                                                                                                                      >  2:4716229/1‑140 (MQ=255)

                         tccACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTAt                                                                                                              <  2:4744015/139‑1 (MQ=255)

                           cACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTAtct                                                                                                            <  1:2665012/140‑1 (MQ=255)

                           cACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTAtct                                                                                                            <  1:1748171/140‑1 (MQ=255)

                                tttGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGtt                                                                                                       >  2:2052925/1‑140 (MQ=255)

                                   cccATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCgg                                                                                                    <  2:4523024/139‑1 (MQ=255)

                                       tgcactgcacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgc                                                                                                >  2:2031479/1‑140 (MQ=255)

                                         ccctggacCTTTATTTGTGACGACGAGACCCGCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAAGGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCGGGCGCTGATTTATCTCGGTTCGGCAgcgc                                                                                              <  2:3608736/138‑1 (MQ=255)

                                         cactgcacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc                                                                                              >  2:3799683/1‑140 (MQ=255)

                                              cacCTTTATTTGTGACGAGGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAtt                                                                                         >  2:1722343/1‑140 (MQ=255)

                                              cacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAtt                                                                                         >  2:835000/1‑140 (MQ=255)

                                              cacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAtt                                                                                         >  1:4752692/1‑140 (MQ=255)

                                              cacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGAtt                                                                                         >  1:1832374/1‑140 (MQ=255)

                                              cacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCGGAtt                                                                                         >  1:843617/1‑140 (MQ=255)

                                              cacCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGATGAtt                                                                                         >  2:1318128/1‑140 (MQ=255)

                                              cacCCTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTAGCCCGGTTCGGCAGCGCTGAtt                                                                                         <  2:3179263/140‑1 (MQ=255)

                                                ccTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGt                                                                                       <  1:629048/140‑1 (MQ=255)

                                                 ctgtATTTGTGACGACGAGAGCCTCGAAGGGCAGGCGCGTTACTATAAGCAACGTCTGCGGGAATGGCAGGAGGCCCGTGATGGAGAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTa                                                                                      <  2:3219392/137‑1 (MQ=255)

                                                 cTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAgcgc                                                                                              <  2:4197299/132‑1 (MQ=255)

                                                   ttATTTGTGACGACGAAAGCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCAGCTGGAATGGCAGGAGGCACATCATGGATAGCCAAACGCTGATTCAGACGCTGATGTATCTCGGTTCGGGAGGGCTGATTATAcc                                                                                    >  2:2688153/1‑140 (MQ=255)

                                                   ttATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc                                                                                    >  1:4203868/1‑140 (MQ=255)

                                                   ttATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTAcc                                                                                    >  1:3909003/1‑140 (MQ=255)

                                                   ttATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCGGATTTATCTCGGTTCGGCAGCGCTTATTGTagc                                                                                    >  2:3238361/1‑138 (MQ=255)

                                                   tgagttGTGACGCCGAACCCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCACGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGGTCGGCAGCGCTGATTGTAcc                                                                                    <  2:2901182/136‑1 (MQ=255)

                                                      tttGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCAt                                                                                 >  2:2304816/1‑140 (MQ=255)

                                                      tttGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCAt                                                                                 <  2:222981/140‑1 (MQ=255)

                                                        tgtgACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTg                                                                               >  1:472081/1‑140 (MQ=255)

                                                        tgtgACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTg                                                                               >  2:3150856/1‑140 (MQ=255)

                                                          tgACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCg                                                                             <  1:4888225/140‑1 (MQ=255)

                                                           gacgacGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg                                                                            >  1:5063749/1‑140 (MQ=255)

                                                           gacgacGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg                                                                            >  2:1648845/1‑140 (MQ=255)

                                                           gacgacGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCgg                                                                            >  1:212608/1‑140 (MQ=255)

                                                            gcgccgaCACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATGCGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGt                                                                           <  2:1093649/136‑1 (MQ=255)

                                                            acgacgAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGt                                                                           <  1:4500787/140‑1 (MQ=255)

                                                            acgacgAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGt                                                                           <  2:2611401/140‑1 (MQ=255)

                                                             cgacgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTa                                                                          <  1:4283354/140‑1 (MQ=255)

                                                                cgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGGTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGAACCCATTGCGGTACGt                                                                       >  1:2820006/1‑140 (MQ=255)

                                                                cgaAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGt                                                                       >  1:1557458/1‑140 (MQ=255)

                                                                  aaaCCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt                                                                     <  2:2228143/140‑1 (MQ=255)

                                                                  aaaCCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt                                                                     <  2:5015322/140‑1 (MQ=255)

                                                                  aaaCCCGCGGGGGGCCCGCGCGTCACTATGAGCACCGTCTGCTGGAATGGCAGGAGGCCCATCATGGCTAGCCATCCGCTGCTTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCt                                                                     <  2:2541933/140‑1 (MQ=255)

                                                                        tCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCt                                                               >  1:1941214/1‑140 (MQ=255)

                                                                         cGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTg                                                              >  2:4366366/1‑140 (MQ=255)

                                                                             gggCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCGGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGAt                                                          <  2:3921971/140‑1 (MQ=255)

                                                                                cAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCgg                                                       >  1:2935530/1‑140 (MQ=255)

                                                                                 aGGCGCGTCACTATACGCAACGTCTGCTGGAATGGCAGGAGGCCCATCTTGGATAGCCACACGCTGCTTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGt                                                      <  2:2158151/140‑1 (MQ=255)

                                                                                  ggCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTa                                                     >  1:1246448/1‑140 (MQ=255)

                                                                                       gTCACTATCACCAACGTCTGCTCGCATGGCAGGAGGCCCATCATGGATACCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTgg                                                <  2:1280207/140‑1 (MQ=255)

                                                                                        tCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGc                                               >  2:3556546/1‑140 (MQ=255)

                                                                                              taAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTGTGGGATCGGTACTTGGCTACCTg                                         >  2:4846289/1‑140 (MQ=255)

                                                                                                   aaCGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGc                                    >  1:1119600/1‑140 (MQ=255)

                                                                                                    aCGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGcc                                   >  2:531404/1‑140 (MQ=255)

                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATGATGGATAGCCATACGCTGATCCAGGCGCTGATTTATCTCGGTTCGTCAGCGCTGACGGTACCCATTGCGGTACGTCTAGGTCTGGGATCGATACTTGGCTAGCAGATCGCCg                                  >  2:3730767/1‑140 (MQ=255)

                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCGATTGCGGTACGTCTCGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg                                  >  2:4690116/1‑140 (MQ=255)

                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg                                  >  1:3514695/1‑140 (MQ=255)

                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg                                  <  2:4203868/140‑1 (MQ=255)

                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg                                  >  2:5078288/1‑140 (MQ=255)

                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg                                  >  1:286924/1‑140 (MQ=255)

                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg                                  >  1:4456650/1‑140 (MQ=255)

                                                                                                     cGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTGGGCTCCCTGATCGCCg                                  >  2:490195/1‑140 (MQ=255)

                                                                                                     cGTCTGCTGGAAGGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCg                                  >  2:5024632/1‑140 (MQ=255)

                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCgg                                 >  2:2240676/1‑140 (MQ=255)

                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCgg                                 >  2:4936330/1‑140 (MQ=255)

                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCgg                                 >  2:792268/1‑140 (MQ=255)

                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCgg                                 >  1:1883496/1‑140 (MQ=255)

                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCgg                                 >  1:1857425/1‑140 (MQ=255)

                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCgg                                 >  1:452249/1‑140 (MQ=255)

                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCgg                                 >  1:517973/1‑140 (MQ=255)

                                                                                                      gTCTGCTGGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCCTGGTCTGGGATCGGTACTTGGCTACCTGAGCGCCgg                                 >  2:5084466/1‑140 (MQ=255)

                                                                                                         tgctgGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTg                              <  1:957110/140‑1 (MQ=255)

                                                                                                         tgatggcatggcaGGAGGCCCATCATGGCTAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTg                              <  2:2677541/137‑1 (MQ=255)

                                                                                                          gctgGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGc                             >  1:2980616/1‑140 (MQ=255)

                                                                                                          gctgGAATGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGc                             >  1:2431149/1‑140 (MQ=255)

                                                                                                                aTGGCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGAGCGCCGGCTGCattatt                       >  1:3702984/1‑140 (MQ=255)

                                                                                                                   gCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTggg                    >  2:4754531/1‑139 (MQ=255)

                                                                                                                   gCAGGAGGCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTggg                    >  1:4494105/1‑139 (MQ=255)

                                                                                                                       gaggCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGt                >  1:581173/1‑140 (MQ=255)

                                                                                                                        aggCCCATCATGGATAGCCATACGCGGATTCAGGCGCTGATTTATCTCGGGTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTg               <  2:2772001/140‑1 (MQ=255)

                                                                                                                         ggCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTgg              >  1:1490262/1‑140 (MQ=255)

                                                                                                                         cgCCCATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGGGGTACGTATTGGTCTGGAATCGAGACTTGTCTACCTGAGTGCAGGCTGCCTCATTGGCCCGtct              >  2:2319232/2‑138 (MQ=255)

                                                                                                                           cccATCATGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTgggg            <  1:903472/140‑1 (MQ=255)

                                                                                                                             catcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTTGGTTCGGCAGCGCTTATTGTACCCATTGCGGTACGTCTTGGTCTGTGATCGGTACATGGCTACCTAATCGCCGGCTGCAATATTTGCTCGTggggac          >  2:178486/1‑138 (MQ=255)

                                                                                                                               tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTg        <  1:2056311/140‑1 (MQ=255)

                                                                                                                               tcatGGATAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTg        <  2:706560/140‑1 (MQ=255)

                                                                                                                                catGGCTAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGc       <  2:820423/140‑1 (MQ=255)

                                                                                                                                     aTAGCCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTg  >  1:4093902/1‑140 (MQ=255)

CAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAACCCTCGAAGGGCAGGCGCGTCACTATAAGCAACGTCTGCTGGAATGGCAGGAGGCCCATCATGGATAACCATACGCTGATTCAGGCGCTGATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTG  >  NZ_CP025268/47641‑47913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`