BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	474,717	C→T	P97S (CCA→TCA)	ybaY →	YbaY family lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	474,717	0	C	T	99.3%	1037.7 / NA	290	P97S (CCA→TCA)	ybaY	YbaY family lipoprotein
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (156/131); minor base G (1/1); total (158/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 292 total aligned reads displayed.

GTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCAGGG  >  NZ_CP025268/474583‑474853

gTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTTATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGtcacca                                                                                                                                     <  1:3892907/140‑1 (MQ=255)

 tACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCAt                                                                                                                                    >  1:4909545/1‑140 (MQ=255)

  aCCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCAtt                                                                                                                                   <  2:711842/140‑1 (MQ=255)

    cGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCa                                                                                                                                 >  1:3189996/1‑140 (MQ=255)

      tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGCCACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGc                                                                                                                               >  1:3168031/1‑140 (MQ=255)

      tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGc                                                                                                                               >  1:1021020/1‑140 (MQ=255)

      tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGc                                                                                                                               >  2:2377253/1‑140 (MQ=255)

      tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGc                                                                                                                               >  1:2681171/1‑140 (MQ=255)

      tCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGGCGTTAGCCGATGCACCGGCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTACACAGTCACCATTCAGc                                                                                                                               >  2:1093174/1‑140 (MQ=255)

       cTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCt                                                                                                                              <  2:1895298/140‑1 (MQ=255)

        tGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCtt                                                                                                                             >  1:4742361/1‑140 (MQ=255)

               gTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTg                                                                                                                      <  2:366135/140‑1 (MQ=255)

               gTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGATTTGTTCTg                                                                                                                      <  2:1245392/140‑1 (MQ=255)

                tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGt                                                                                                                     >  1:1138915/1‑140 (MQ=255)

                tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGt                                                                                                                     >  1:5065480/1‑140 (MQ=255)

                tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGt                                                                                                                     >  1:3554052/1‑140 (MQ=255)

                tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGt                                                                                                                     >  1:166660/1‑140 (MQ=255)

                tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGt                                                                                                                     >  1:1503785/1‑140 (MQ=255)

                tCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGt                                                                                                                     >  1:346527/1‑140 (MQ=255)

                tCAGAAAATTGCACTGCCGCCTGATGCTGTGCTGACCGTGAAACTTTCTGACGCGTCGTTAGACGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATCCAGATTTGTTCTGt                                                                                                                     >  2:2820802/1‑140 (MQ=255)

                gcAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGCCCGTGACACTTTCTGACGCGTCGGTAGCCGATGCACCGTCAAAAGTGCGGGCGCAGATAGCGGTTCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTgg                                                                                                                     >  2:446146/2‑139 (MQ=255)

                           cACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCg                                                                                                          >  1:60486/1‑140 (MQ=255)

                           cACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCg                                                                                                          >  2:5066728/1‑140 (MQ=255)

                                          ctgtgctgACCGTGGCACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATACAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCg                                                                                           >  2:2456046/1‑140 (MQ=255)

                                            gtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGaa                                                                                         <  1:2185488/140‑1 (MQ=255)

                                            gtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGaa                                                                                         <  2:166660/140‑1 (MQ=255)

                                            gtgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTAAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGaa                                                                                         <  1:1460487/140‑1 (MQ=255)

                                            gtgctgACCGTGACACCTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGaa                                                                                         <  1:223966/140‑1 (MQ=255)

                                             tgttgACCGTGAGACTTTCTGGCGCGTCGGTTGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGGCACCAGTCAGCTTTGTTCTGTCATTTAACTCGGCAGATGTTGAGcctgac                                                                                        >  2:1107778/4‑136 (MQ=255)

                                             tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAc                                                                                        >  1:3249414/1‑140 (MQ=255)

                                             tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAc                                                                                        >  2:1773786/1‑140 (MQ=255)

                                             tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAc                                                                                        >  1:1427859/1‑140 (MQ=255)

                                             tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAc                                                                                        >  2:781864/1‑140 (MQ=255)

                                             tgctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGGTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAc                                                                                        >  1:271944/1‑140 (MQ=255)

                                               ctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgc                                                                                      <  2:483066/140‑1 (MQ=255)

                                               ctgACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGAGCAGAAAGCGGTGCGTACTGACGGTAAACAGTCGACATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgc                                                                                      <  2:627385/140‑1 (MQ=255)

                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                    <  2:1774265/140‑1 (MQ=255)

                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                    <  2:3189996/140‑1 (MQ=255)

                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                    <  1:965010/140‑1 (MQ=255)

                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                    >  2:3834302/1‑140 (MQ=255)

                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                    <  1:940420/140‑1 (MQ=255)

                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                    <  1:4676536/140‑1 (MQ=255)

                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAATGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCATATGTTCAGCCGAAcgcgc                                                                                    >  2:4111781/1‑140 (MQ=255)

                                                 gACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCCTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgc                                                                                    <  2:4246661/140‑1 (MQ=255)

                                                 gACCGCGGCACTGTCTGACGCGTCGGTAGCCGATGCACCGTCAAAAGTGCTGGGGCAGTAAGCGGTGCGTACTGAAGGTAACCAGTCACGATTCAGCTTTGTTCTGTCATTTATCCCGGCAGATGTTCAGCCGAAcgcgc                                                                                    <  2:3079989/140‑1 (MQ=255)

                                                  aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgcg                                                                                   >  1:2029357/1‑140 (MQ=255)

                                                  aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgcg                                                                                   >  2:3991254/1‑140 (MQ=255)

                                                  aCCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAAcgcgcg                                                                                   >  1:985831/1‑140 (MQ=255)

                                                   ccGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGt                                                                                  <  2:933705/140‑1 (MQ=255)

                                                       gACACTTTCTGACGCGTCGTTGGCCGATGCACCGTCAAAAGGTCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTGCTGTCATTTAACCGGGCAGATGGTCAGACGGACGCGCGTAtac                                                                              >  2:3524351/1‑138 (MQ=255)

                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTc                                                                              >  1:2498354/1‑140 (MQ=255)

                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTc                                                                              >  1:1541646/1‑140 (MQ=255)

                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTc                                                                              >  1:7742/1‑140 (MQ=255)

                                                       gACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGCGCGTACTGAAGGCAAACAGTCACCATTCTGCTTTGTTCTGTGAATTAACCCAGCAGATGCTCAGCCGGACGCGCGTGTTc                                                                              >  2:2269688/1‑140 (MQ=255)

                                                       gACACTTTCTGACGCGGCATTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAAGCCGGCAGATGTTCAGCCGAACGCGCGTATTc                                                                              >  1:3612058/1‑140 (MQ=255)

                                                            tttCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCGTGTAACCCGGCAGATGTTCAGCCGAACGCGCGTAGTCTGTTg                                                                         >  2:3369808/1‑140 (MQ=255)

                                                                tGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTg                                                                     >  2:2475512/1‑140 (MQ=255)

                                                                 gACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTACCCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgc                                                                    <  1:2913539/140‑1 (MQ=255)

                                                                 gACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgc                                                                    <  2:3315091/140‑1 (MQ=255)

                                                                 gACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCAGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTgc                                                                    <  1:1835894/140‑1 (MQ=255)

                                                                      gtcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGa                                                               <  1:4016130/140‑1 (MQ=255)

                                                                       tcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGAt                                                              >  1:741266/1‑140 (MQ=255)

                                                                       tcgtTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGAt                                                              >  2:2157564/1‑140 (MQ=255)

                                                                       tagtacgCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCCGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGAGGCGAt                                                              <  2:1434820/134‑1 (MQ=255)

                                                                                  tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATg                                                   >  2:3455051/1‑140 (MQ=255)

                                                                                  tGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATg                                                   >  1:850319/1‑140 (MQ=255)

                                                                                    cACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCCTTCAGCTTTGTTCTGTCATTTAACCCGGCAGGTGTTCAGCCGAACGCGCGGAGGCTGTTGCGTGCGGCGATTACCGTGCATgcc                                                 >  2:2978725/1‑138 (MQ=255)

                                                                                     aCCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACa                                                <  1:2650673/140‑1 (MQ=255)

                                                                                     aCCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACa                                                <  2:2049972/140‑1 (MQ=255)

                                                                                     aCCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACa                                                <  1:4951519/140‑1 (MQ=255)

                                                                                        gTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACCGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAAc                                             <  2:1178412/140‑1 (MQ=255)

                                                                                          cAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTg                                           <  1:3531093/140‑1 (MQ=255)

                                                                                           aaaaGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTgg                                          >  2:2768733/1‑140 (MQ=255)

                                                                                            aaaGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGt                                         >  1:2425581/1‑140 (MQ=255)

                                                                                            aaaGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGt                                         <  2:1965591/140‑1 (MQ=255)

                                                                                                tGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTACCCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGCTTACCGTGAATGACAAACTGGTAttt                                     <  2:157212/140‑1 (MQ=255)

                                                                                                tGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTAttt                                     <  2:732227/140‑1 (MQ=255)

                                                                                                ggCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCTCCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTAttt                                     <  2:4402635/139‑1 (MQ=255)

                                                                                                  cTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTGACTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTAt                                   <  1:4221666/140‑1 (MQ=255)

                                                                                                   tGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATc                                  >  1:2014887/1‑140 (MQ=255)

                                                                                                        cAGAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGa                             <  2:4624237/140‑1 (MQ=255)

                                                                                                          gAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGCCAAACTGGTATTTATCACCGATa                           >  2:1276523/1‑140 (MQ=255)

                                                                                                          gAAAGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATa                           >  1:4790660/1‑140 (MQ=255)

                                                                                                           aaaGCGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATAc                          <  1:897102/140‑1 (MQ=255)

                                                                                                               cGGTGCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGtt                      <  2:4901746/140‑1 (MQ=255)

                                                                                                                 gTGCGTACTGAAGGTAAACAGTCACCCTTCGGCTTTGTTCTGTCATTTAACGCGGCAGATGTTCAGCCGAACGCGTGTATTCTGTTGAGTCCGACGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCa                    >  1:3054879/1‑140 (MQ=255)

                                                                                                                   gCGTACTGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGc                  <  2:1427859/140‑1 (MQ=255)

                                                                                                                         tGAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGa            <  1:4580565/140‑1 (MQ=255)

                                                                                                                          gAAGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGAt           <  1:1012156/140‑1 (MQ=255)

                                                                                                                            aGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCa         >  1:1269161/1‑140 (MQ=255)

                                                                                                                            aGGTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCa         >  1:2580823/1‑140 (MQ=255)

                                                                                                                              gTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCTGCCGGTGATCAAc       >  1:4476376/1‑140 (MQ=255)

                                                                                                                              gTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAAc       <  2:4179724/140‑1 (MQ=255)

                                                                                                                              gTAAACAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAAc       <  1:3723344/140‑1 (MQ=255)

                                                                                                                                aaaCAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCa     >  1:3657239/1‑140 (MQ=255)

                                                                                                                                aaaCAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCa     >  1:2865073/1‑140 (MQ=255)

                                                                                                                                aaaCAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAAGGACAAACTGGGATTTATCACCGATACCGTTCAGCCGGGTATCAACCa     >  2:3898861/1‑140 (MQ=255)

                                                                                                                                 aaCAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCTGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCAg    >  1:4409942/1‑140 (MQ=255)

                                                                                                                                 aaCAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCAg    >  1:3810087/1‑140 (MQ=255)

                                                                                                                                 aaCAGTCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCAg    >  2:3251658/1‑140 (MQ=255)

GTACCGTCTGGATCCGTCAGAAAGTCGCACTGCCGCCTGATGCTGTGCTGACCGTGACACTTTCTGACGCGTCGTTAGCCGATGCACCGTCAAAAGTGCTGGCGCAGAAAGCGGTGCGTACTGAAGGTAAACAGCCACCATTCAGCTTTGTTCTGTCATTTAACCCGGCAGATGTTCAGCCGAACGCGCGTATTCTGTTGAGTGCGGCGATTACCGTGAATGACAAACTGGTATTTATCACCGATACCGTTCAGCCGGTGATCAACCAGGG  >  NZ_CP025268/474583‑474853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`