Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 489,335 T→C *176* (TAA→TAG)  priC ← primosomal replication protein N''

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268489,3350TC99.3% 1012.3 / NA 303*176* (TAA→TAG) priCprimosomal replication protein N''
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (195/106);  minor base A (2/0);  total (197/106)
Fisher's exact test for biased strand distribution p-value = 5.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 303 total aligned reads displayed.

ATAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGG  >  NZ_CP025268/489199‑489471
                                                                                                                                        |                                                                                                                                        
aTAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAg                                                                                                                                       <  1:2022865/140‑1 (MQ=255)
     cACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGt                                                                                                                                  >  2:1796281/1‑140 (MQ=255)
            tccAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCTTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTACCGGGCTAAAcgc                                                                                                                           >  2:3145793/3‑140 (MQ=255)
            tACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAAcgc                                                                                                                           >  1:3776788/1‑140 (MQ=255)
             aCAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAAcgcg                                                                                                                          <  1:1457249/140‑1 (MQ=255)
                  atCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAAc                                                                                                                     >  1:3377524/1‑140 (MQ=255)
                  atCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATCTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAAc                                                                                                                     >  2:1165131/1‑140 (MQ=255)
                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCTTCCAGCAGCACAATCGAATTGACAGCCAGTTTTCAATCATCAGGGGAATTGTCCAGCGACATATTCTCTCCATTCATAGCTGGTGACACGCACTAACCTGt                                                                                                                 >  2:2545814/1‑140 (MQ=255)
                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGt                                                                                                                 >  1:3345823/1‑140 (MQ=255)
                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGt                                                                                                                 >  1:4838841/1‑140 (MQ=255)
                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGt                                                                                                                 >  1:283829/1‑140 (MQ=255)
                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGt                                                                                                                 >  1:2620451/1‑140 (MQ=255)
                      aGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTAAAGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGt                                                                                                                 >  1:4232472/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGGTTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  2:1546360/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCGGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACGTGttt                                                                                                               >  2:4485546/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCTCTAACCTGttt                                                                                                               >  2:2677053/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  1:1039710/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  1:2419220/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  2:660402/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  2:4712173/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  1:1675589/1‑140 (MQ=255)
                        cgcgcgcAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGttt                                                                                                               >  1:1153243/1‑140 (MQ=255)
                              cagcaCGGTACTGGCAGGGATCTGATTTTTCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGAtt                                                                                                         <  1:4611509/140‑1 (MQ=255)
                                   cGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTc                                                                                                    <  2:2251886/140‑1 (MQ=255)
                                      tACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACGTCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCAGTTTTCGATTTTTTCCAg                                                                                                 >  2:3642676/1‑140 (MQ=255)
                                        ctggtcgggATCTGAATTACCCCCAGAAGCTTAATCAAATCGACAGCCAGTTTGACCTCGTCACGGGCATTTTCCAGTGACATATACTCTCCAATGCTAGCGGGTTAAACGCGCTAACCTGTCTTCGATTTTTTCCAgcg                                                                                               <  2:1814419/134‑1 (MQ=255)
                                        cTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAgcg                                                                                               <  1:3054237/140‑1 (MQ=255)
                                           gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCCGTTTGCCATCTTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCAt                                                                                            >  2:566827/1‑140 (MQ=255)
                                           gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCAt                                                                                            >  1:1538684/1‑140 (MQ=255)
                                           gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCAt                                                                                            >  1:1349039/1‑140 (MQ=255)
                                           gCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCAt                                                                                            >  1:4158774/1‑140 (MQ=255)
                                              gggATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggc                                                                                         >  2:1221275/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCGGCGCAATCAAATCGACAGCCCGGTTGACGTCGGCAGGGGCCTTTTGCAGTGACATATTCTCTCCATTGCTAGCGGGTTGAACGCGATAACCTGTTTTCGATTTTTTCCAGCGCATggcgc                                                                                       >  2:2642249/1‑139 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAGTCAAATCGACAGCAAGTTTGACATCAACAGGGGCATTTTCCTGCGACATTAGCTCTCCAGTAAGATCGGATTACACGCGCTAACCGGTTTTCATTTTCTTCCAgcgcttcgggg                                                                                       >  2:1670523/1‑133 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:812788/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:1457029/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:103037/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:4119378/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:2951685/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:4041129/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:3879899/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:3332979/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:3452456/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:3853366/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:29983/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  1:4508077/1‑140 (MQ=255)
                                                gATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGAATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATggcgg                                                                                       >  2:3048715/1‑140 (MQ=255)
                                                   cTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAg                                                                                    <  2:4910310/140‑1 (MQ=255)
                                                   cTGATTTTCCTACAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAg                                                                                    <  1:2212268/140‑1 (MQ=255)
                                                     gATTTTCCTCCATCAGCACAATCAAATCGACAGCCAGTTTGACATCGTGAGGGGCATTTTCCAGTTACATATGCTGTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTGCAGCGCATGGCGGCAGCg                                                                                  >  2:196325/1‑140 (MQ=255)
                                                     gATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCg                                                                                  >  1:1907930/1‑140 (MQ=255)
                                                           cctccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAg                                                                            >  1:630939/1‑140 (MQ=255)
                                                           cctccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAg                                                                            >  1:256240/1‑140 (MQ=255)
                                                           cctccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAg                                                                            >  1:3593548/1‑140 (MQ=255)
                                                              ccAGCAGCATAATCAAACCTACAGCCAGTGTGACATTTTCAGGGGCATTTTCCAGTGATATATTCTCTCCCTCGCTAGCGGGTCAGGAGCGCTAACCTGTTTGTGAGTGTTTCCAGCCCATGGCGGCAGCGTGCCAGgcg                                                                         <  2:2517424/140‑1 (MQ=255)
                                                              ccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         <  2:485045/140‑1 (MQ=255)
                                                              ccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         <  2:4612004/140‑1 (MQ=255)
                                                              ccAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         <  2:2916979/140‑1 (MQ=255)
                                                              ccAGCAGCACAATCAAAGCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGCTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcg                                                                         <  1:864380/140‑1 (MQ=255)
                                                                ggcactacaATCAGATCCCCAGTCCGCTTCACATCGTGTGGGTCATTTACCAGTGACATATTCTCTCCATTGCTAGCGTGTTACACGCGCCGCCCTGTTTGCTATTATTTCCAGCGCACGTCGGCAGCGTGCCAGgcgcg                                                                       <  2:1508193/134‑1 (MQ=255)
                                                                agcagcACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgcg                                                                       <  1:2937535/140‑1 (MQ=255)
                                                                agcagcACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGgcgcg                                                                       >  2:4342016/1‑140 (MQ=255)
                                                                     cacaATCAAATGGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTTCATTGCTAGCGGGTTAAACGCCGTAACCTGTTTTCGATTTTTTCCAGCCCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                                  <  2:712268/140‑1 (MQ=255)
                                                                     cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                                  <  1:4477349/140‑1 (MQ=255)
                                                                     cacaATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCa                                                                  <  2:5014232/140‑1 (MQ=255)
                                                                          tCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGc                                                             >  1:2311817/1‑140 (MQ=255)
                                                                          tCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGc                                                             <  1:4977195/140‑1 (MQ=255)
                                                                          tCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGc                                                             <  1:1104778/140‑1 (MQ=255)
                                                                             aaTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCCGTGCCATATTCTCTCCCTTGCTAGCGGGTTAAACGCGCTAACCTGTTCTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCtgc                                                          <  2:2343853/140‑3 (MQ=255)
                                                                             aaTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTc                                                          <  2:965596/140‑1 (MQ=255)
                                                                             aaTCGACAGCCAGATTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATCGCTAGCGGGTTAAACGCGCTAACCTGTTCTCGATTCTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTc                                                          <  2:2847065/140‑1 (MQ=255)
                                                                              aTCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTcc                                                         >  1:956932/1‑140 (MQ=255)
                                                                                    acacAGTTTGACATCGTCCGCGGCATTTTCCAGTGACATATTCCCTCCCTTGCTAGCTGGTTAAACGCGCTAACCTGTTTTCGCTTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCa                                                   <  2:3283212/137‑1 (MQ=255)
                                                                                       cAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGA‑TTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGAt                                               <  2:3359578/140‑1 (MQ=255)
                                                                                         gTTTGACATCGTCAGGGGCACTTTCCAGTGGCATATTCTCTCCATTTCTAGCGTGTTAAACGCGCTGACCTGCTTGCTATTCTCCCCAGCCCTTGTCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATg                                              <  2:2019297/140‑1 (MQ=255)
                                                                                          tttGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATTGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGc                                             <  2:4433081/140‑1 (MQ=255)
                                                                                          tttGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTCCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGc                                             <  1:4034297/140‑1 (MQ=255)
                                                                                          ttcggcATCGGCAGGGGCACTTGCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAACCGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGGGCTTCATAGGCTTCCACTTCACGATGc                                             <  2:4155822/135‑1 (MQ=255)
                                                                                             gACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAATGCGCTAACGTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGc                                          <  2:3726953/140‑1 (MQ=255)
                                                                                                   gTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTCTCCAGCGCATGGCGGCAGCGTGCCCGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGc                                    <  1:2797336/140‑1 (MQ=255)
                                                                                                    tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCt                                   <  2:1106031/140‑1 (MQ=255)
                                                                                                    tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCt                                   >  1:4407091/1‑140 (MQ=255)
                                                                                                    tCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCt                                   >  1:3131596/1‑140 (MQ=255)
                                                                                                     cAGGGGCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGATAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTg                                  <  1:1595764/140‑1 (MQ=255)
                                                                                                        gggCATTTTCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTc                               <  2:609501/140‑1 (MQ=255)
                                                                                                             ttttCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACga                          >  1:2355395/1‑140 (MQ=255)
                                                                                                             ttttCCAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACga                          <  1:1027362/140‑1 (MQ=255)
                                                                                                              tttACAGTGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACgag                         >  2:2967573/1‑140 (MQ=255)
                                                                                                                     tGACATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTc                  >  1:1384968/1‑140 (MQ=255)
                                                                                                                       aCATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCAc                >  2:1524942/1‑140 (MQ=255)
                                                                                                                       aCATATTCTCTCCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCAc                <  1:1221275/140‑1 (MQ=255)
                                                                                                                        cATATTCTCCTCCTGGCTAGCGGGTTAAACGCGCTACCCCGTTTTCGCTTTTTTCCACCGCACGGCGGCAGCGTGCCAGGCGCGCTTCCTAGGCTTCCACTTCCAGAGGCAGCGTTTGCTGTTCCACGCGATCGGTCAcc               <  2:2171863/140‑1 (MQ=255)
                                                                                                                             tctcgcCATTGCTAGCGTGTTAAACGCGCTAACCTCTTTTCCATTTTTTCCAGCGCCTGCCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACCATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCgcgc          <  2:384737/140‑1 (MQ=255)
                                                                                                                               tatcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCTCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCTGTCACCCGCGCCa        >  2:933911/3‑140 (MQ=255)
                                                                                                                                 tcCATTGCTAGCGGGTTCAACGCGCTCACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCCCCCGCGCCAGa      >  2:3931293/1‑140 (MQ=255)
                                                                                                                                 tcCATTGCTAGCGGGTTAAACGCGCTAGTGTGTTTTCGGTTTTATCCAGGGCATGGTGGCGTCGTGCCTGGCTCGCTTCATAGGCTTTCGGTGCACGATGCAGCGTTTGCTGTTCTATGAGATCGGGCACGTGCGCCAgt      >  2:1229248/1‑139 (MQ=255)
                                                                                                                                 tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAgc      >  1:2447740/1‑139 (MQ=255)
                                                                                                                                 tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa      >  2:1856424/1‑140 (MQ=255)
                                                                                                                                 tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa      >  1:1316249/1‑140 (MQ=255)
                                                                                                                                 tcCATTGCTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGa      >  1:730612/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
ATAATCACGCTTTACAATATCCAGCGCGCGCAGCACGGTACTGGCAGGGATCTGATTTTCCTCCAGCAGCACAATCAAATCGACAGCCAGTTTGACATCGTCAGGGGCATTTTCCAGTGACATATTCTCTCCATTGTTAGCGGGTTAAACGCGCTAACCTGTTTTCGATTTTTTCCAGCGCATGGCGGCAGCGTGCCAGGCGCGCTTCATAGGCTTCCACTTCACGATGCAGCGTTTGCTGTTCCACGAGATCGGTCACCCGCGCCAGACGGG  >  NZ_CP025268/489199‑489471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑